Plant Productions - Articles in Press

The Study on the Hexane and Ethanol Extracts Components in Difference Varieties of Narcissus (Narcissus tazetta L.)

Mohammad Younis Mahen; Mohammad Mahmoodi Sourestani; Hossein Motamedi; Seyyed Mansour Seyyednejad

Volume 45, Issue 3 , December 2022, , Pages 323-333

https://doi.org/10.22055/ppd.2022.40498.2027

Abstract

IntroductionNarcissus (Narcissus tazetta L.) belongs to the family of Amaryllidaceae is one of the most important ornamental and aromatic plants which its secondary metabolites are ... Read More IntroductionNarcissus (Narcissus tazetta L.) belongs to the family of Amaryllidaceae is one of the most important ornamental and aromatic plants which its secondary metabolites are being used in cosmetic, sanitary and perfume industries. Behbahan district belongs to Khuzestan province is one of the well-known narcissus production centers in Iran. Narcissus plants not only have ornamental value, but also have medicinal compounds such as alkaloids that have antiviral and anti-tumor properties. Numerous experiments have been identified the components of Narcissus essential oil and the most significant of aroma constituents have been reported as trans-β-Ocimene, Benzyl acetate, 1, 8-cineole, linalool and phenyl ethyl acetate. However, there is little information about Narcissus extract and the current study was performed to investigate the compounds of ethanol and hexan extracts in different varieties of narcissus. Materials and MethodsThis research was conducted in randomized complete design to evaluate components of hexane and ethanol extracts of five narcissus varieties (Shahla, Meskinack, Porpar, Panjegorbai and Golsefied) which are grown in Behbahan district. The fresh flowers of narcissus were extracted using tow solvents; hexane and ethanol. In the hexane extraction method, 10 g of the fresh flowers were weighed from each variety and poured into Erlenmeyer, and then 100 ml of hexane was added to each one and left at room temperature for 10 days. After extraction, the solvent was filtered and the extract was concentrated by vacuum rotary and absolute was obtained. Then 10 times of ethanol was added to the absolute of each variety and kept at -4 °C to precipitate the wax. After precipitation, the supernatant was separated and concentrated again by rotary and then the components were detected by GC/MS. In the ethanol extraction method, also 10 g of the fresh flowers were also weighed from each variety and poured into Erlenmeyer, and then 100 ml of ethanol was added to each one and left at room temperature for 10 days. After extraction, the solvent was filtered and the extract was concentrated by vacuum rotary and absolute was obtained. The rest of the steps are similar to the hexane method which is described above. Results and DiscussionAccording to the results, the maximum amount of hexane extract (1.3%) and the minimum amount (0.9%) were observed in variety of Shahla and in Porpar, respectively. According to the results obtained from the analysis of the extract by GC/MS, approximately 7 to 12 compounds have been identified in narcissus varieties. Benzyl acetate, Linalool, Benzyl alcohol, trans -β-Ocimene, Hydrocinnamyl alcohol, Phenyl ethyl alcohol, 1, 3 Dimethyl ethyl benzene were the most important components. In ethanol extract, the maximum amount of ethanol extract (6.2%) and the minimum amount (4.8%) were recorded in variety of Shahla and in Porpar, respectively. Interestingly, more compounds were identified in ethanol than hexane extract. The most important compounds were 2-butoxy ethanol, 1, 8-Cineol, Benzyl alcohol, trans-β-Ocimene, Linalool, Phenyl ethyl alcohol, Benzyl acetate, Benzene ethanol and Benzene which were recorded in all varieties of narcissus. ConclusionIn general, the results showed that there is a significant difference in terms of yield, number and percentage of chemical compounds of hexane and ethanol extracts of different varieties of narcissus. According to the results, the maximum amount of hexane and ethanol extract was obtained in Shahla while the minimum amount was observed in Porpar. Benzyl acetate, Linalool, Benzyl alcohol, trans-β-Ocimene, Phenyl ethyl alcohol, 1, 3 Dimethyl ethyl benzene are the most important components of both extracts. In order to isolate extract components and report exclusive compounds, further experiments are recommended. Keywords: Benzyl acetate, Extract, Gas chromatography, Linalool, Narcissus, Organic Solvents

Effect of the Vertical and Non-Vertical Spray of Flat Fan Nozzle Types on the Control Efficacy of Winter Wild Oat (Avena sterilis subsp. ludoviciana Durieu.) by Haloxyfop-R-methyl

Akbar Aliverdi; Samira Karami

Volume 44, Issue 3 , December 2021, , Pages 331-344

https://doi.org/10.22055/ppd.2020.32527.1875

Abstract

AbstractBackground and ObjectivesSince the leaves of a grass species are perpendicularly oriented to the ground, spraying an ACCase inhibitor with a conventional hydraulic nozzle can ... Read More AbstractBackground and ObjectivesSince the leaves of a grass species are perpendicularly oriented to the ground, spraying an ACCase inhibitor with a conventional hydraulic nozzle can not cause an adequate control. In such a situation, it is well established that a large endo-drift of the spray droplets can be occurred. Recently, a new spray technique, angled spray, has been introduced to maximize the deposition of spray droplets on a vertical target surface (i.e., grassy species). This technique is simple, inexpensive, and available to farmers. Therefore, it can be noteworthy to them. This reserech aimed to study the effect of many angled sprays by three flat fan nozzle types on the efficacy of haloxyfop-R-methyl against winter wild oat.Materials and MethodsThis study was conducted as dose-response bioassay at the research greenhouse of Bu-Ali Sina University in 2019. Haloxyfop-R-methyl at seven doses (0, 5.06, 10.12, 20.25, 40.5, 60.75, and 81 ml a.i. ha-1) were sprayed at 300 kPa spray pressure using three flat fan nozzle types having different atomization quality (standard (Fine: 144–235 µm), anti-drift (Medium: 236–340 µm), and air induction (Extremely Coarse: 503–665 µm)) in seven angled sprays (vertical spray and non-vertical sprays with 10, 20, 30, 40, 50, and 60° forward) on winter wild oat at the three-leaf stage. The haloxyfop-R-methyl dose required to give a 50% reduction in dry weight of winter wild oat (ED50) was detected. ResultsThe ED50 values were significantly affected by the nozzle type and the spray angle. With a vertical spray, the ED50 values of 32.51, 44.05, and 53.98 ml a.i. ha-1 was obtained from the flat fan nozzles of standard, anti-drift, and air induction, respectively. The best efficacy of haloxyfop-R-methyl was obtained using a 30° forward angled spray by standard flat fan nozzle (ED50 = 15.10 ml a.i. ha-1), a 40° forward angled spray by anti-drift flat fan nozzle (ED50 = 17.13 ml a.i. ha-1), and a 50° forward angled spray by air induction flat fan nozzle (ED50 = 17.61 ml a.i. ha-1). DiscussionFrom this experiment, the following points were obtained: 1) with a vertical spray, there is a negative correlation between the herbicide efficacy and the spray droplet size; 2) with a non-vertical spray, there is a neutral correlation between the herbicide efficacy and the spray droplet size; 3) when the droplets produced by a nozzle is larger, the more non-vertical spray is required to obtain maximoum herbicide efficacy.

The Alleviation of the Adverse Effects of Salinity Stress in the Tomato (Solanum lycopersicum L. cv. Mobin) by Application of Ascorbic Acid

Bahareh Hajivar; Mohammad reza Zare bavani

Volume 43, Issue 3 , November 2020, , Pages 349-362

https://doi.org/10.22055/ppd.2019.27610.1672

Abstract

Abstract Background and Objectives Salinity stress is one of the major environmental factor limiting plant growth and productivity. The adverse influence of salinity stress is ... Read More Abstract Background and Objectives Salinity stress is one of the major environmental factor limiting plant growth and productivity. The adverse influence of salinity stress is evaluated on all plant levels. Ascorbic acid is a small, water-soluble antioxidant molecule that acts as a primary substrate in the pathway for detoxification and neutralization of superoxide radicals and singlet oxygen. Ascorbic acid has been shown to play multiple roles in plant growth, such as in cell division, cell wall expansion, and other developmental processes. The objective of the present experiment was to investigate the effects of the exogenous application of ascorbic acid on the growth parameters and photosynthesis attributes of tomato plants. Materials and Methods The experiment was conducted as a factorial in a completely random design with three replications in greenhouse conditions. Salinity stress was applied by 0, 25, 50, 100 mM NaCl in modified Hoagland solution. Ascorbic acid treatments (0, 2.5 and 5 mM) were sprayed on the leaves every three days until the end of experiment. Leaf, stem, and root fresh and dry weight, total fresh and dry weight, yield per plant, net photosynthesis, Stomata conductance, transpiration, internal CO2 concentration, chlorophyll a, b and total chlorophyll, proline and total soluble sugar content were evaluated. Results In the present experiment, salinity stress sharply reduced the leaf, stem, root and total fresh and dry weight, yield per plant, net photosynthesis, stomata conductance, transpiration, internal CO2 concentration, chlorophyll a, b and total chlorophyll content whereas the exogenous treatment of AsA appreciably delayed the loss of all studied traits. Foliar application of ascorbic acid under both stress and non-stress conditions improved the growth parameters and photosynthesis attributes. Salinity stress also increased total soluble sugar content and proline accumulation in the leaf of the tomato plant. Application of ascorbic acid under both non-stress and salinity stress conditions decreased total soluble sugar content and proline accumulation and reduced the effects of salinity stress. Discussion This experiment showed that treating the tomato (Solanum lycopersicum L. cv. Mobin) with 5 mM ascorbic acid substantially influences several metabolic processes, leading to the increased growth. The protection of the plant against salinity stress by using of ascorbic acid was possibly to be caused indirectly as a result of increasing amount of chlorophyll which plays an essential role in photosynthesis process and then formation of carbohydrates. Thus, it might be concluded that exogenously applied ascorbic acid has low external osmotic potential and ion toxicity and it might be effective in amelioration of the adverse effects of salt stress.

Optimization of antibiotics during co-culture of Petunia hybrida leaf explants and Agrobacterium tumefaciens to increase gene transgenic efficiency

Azadeh Khadem; zahra sargazi moghaddam; Mahboobeh yazdi; Mahdiyeh Kharrazi; Ahmad Sharifi

Volume 46, Issue 3 , December 2023, , Pages 353-366

https://doi.org/10.22055/ppd.2024.43645.2099

Abstract

IntroductionGene transfer by the Agrobacterium tumefaciens is one of the well-known methods for the production of the transgenic plants. The success rate of this method depends on the ... Read More IntroductionGene transfer by the Agrobacterium tumefaciens is one of the well-known methods for the production of the transgenic plants. The success rate of this method depends on the growth of the bacteria and the regeneration of the plant explants. Among these variables, the antibiotics functioning as bacterial growth regulators have mainly inhibitory effects on the plant growth. Therefore, the introduction of a successful gene transfer method which is mediated by Agrobacterium requires the identification of an antibiotic with an inhibitory effect on the growth of the bacteria and also has a minimal effect on the regeneration of the explants and the growth of the transgenic plants. In this regard, the present research was conducted with the aim of optimizing the antibiotic used in co-culture in order to increase the transgenic yield of Petunia hybrida. For this purpose, a variety of antibiotics in different concentrations were tested.Materials and MethodsThe growth rate of A. tumefaciens in the different medium culture with two suitable pH for the bacterial growth (7) and plant growth (5.7) in the presence of 500 mg/L of the cefotaxime antibiotic as a common antibiotic for controlling bacterial growth was investigated. Sensitivity tests were run. The second experiment was conducted to investigate the effect of five different antibiotics in three concentration on the regeneration of the petunia leaf explants. Results and DiscussionThe results of the bacterial sensitivity test showed that the type and concentration of salt and the pH of the medium culture significantly affect the growth rate of the bacteria. So that the highest rate of bacterial growth was observed in LB and MH medium culture at pH=7, but on the contrary, no growth was observed in MS medium culture. In addition, two antibiotics of cefotaxime and ceftriaxone at 700 mg/L concentration had the highest inhibitory effect on the bacterial growth. The results of this experiment showed that different antibiotics have different effects on the regeneration of explants and the growth of the seedlings. According to the results, the highest volume of callus and also the highest percentage of regeneration were observed in the presence of ceftizoxime and coamoxiclav antibiotics in all three concentrations and cefepime at 300 mg/L. The highest number of the seedlings was also observed in the treatment of ceftizoxime at concentrations of 300 and 500 mg/L with an average of 7.70 seedlings. In relation to the seedling growth traits, the highest number and length of root per seedling were observed in ceftizoxime at 300 mg/l and cefotaxime at the same concentration, respectively. The highest dry weight belonged to the grown seedlings in the presence of co-amoxiclav antibiotic followed by the ceftizoxime (300 mg/L).Conclusion The overall results of these experiments show that the seedlings which are grown in the presence of ceftizoxime antibiotic had a good growth rate, while the use of this antibiotic was effective in inhibiting the growth of A. tumefaciens. Therefore, we recommend that the use of ceftizoxime antibiotic in the co-culture process will be effective in increasing the efficiency of the gene transfer. Further studies will solidify these results..

Improvement of Anthocyanin and Antioxidant Properties of Strawberry (cv. Amaros) by Calcium Lactate and Potassium Sorbate Application

Farah Hosseini; Mohamd Esmail Amiri; Farhang Razavi

Volume 42, Issue 4 , December 2019, , Pages 455-468

https://doi.org/10.22055/ppd.2019.25261.1580

Abstract

Abstract Background and Objectives Strawberry (Fragaria × ananassa Duch.) is one of the most popular soft fruits worldwide. It is a rich source of health promoting compounds ... Read More Abstract Background and Objectives Strawberry (Fragaria × ananassa Duch.) is one of the most popular soft fruits worldwide. It is a rich source of health promoting compounds such as essential macro/micro-nutrients, polyphenols, anthocyanin, vitamins and amino acids, while showing a high antioxidant capacity. Preharvest applications of calcium lactate and potassium sorbate have shown to be promising as a tool to improve the quality and extend the shelf life of various fruits. To our knowledge, however, little information is available regarding the effect of calcium lactate and potassium sorbate on strawberry fruits. Thus, the objective of the present study was to determine the effect of foliar application of calcium lactate and potassium sorbate on important quality characteristics and antioxidant properties of ‘Armas’ strawberries. Materials and Methods This research was performed at a commercial orchard on 2-year old strawberries in Marivan, Iran in 2015. The experiment was arranged as factorial based on randomized complete block design with two factors and four replications. The first factor included different levels of calcium lactate (0, 0.5 and 1%) and the second factor included different levels of potassium sorbate (0, 0.5 and 1%). Foliar spraying was performed in three stages: three weeks before commercial harvesting, at initial coloring, and one week before harvest. Fruit were harvested at commercial maturity and immediately transported to the laboratory at University of Zanjan and subjected to physicochemical analysis. Differences among means of data were analyzed by Duncan’s test at p ≤ 0.05 (n = 3). All statistical analyses were performed with SAS version 9.4. Results The results showed that the combination of calcium lactate and potassium sorbate (0.5 Percent concentration) treatment significantly (P £ 0.01) increased the total anthocyanin content of the whole fruit, reuslting into further attractive fruit color and market than the control. It also increased antioxidant properties up to a level of 57.07%. On the other hand, the application of this treatment (calcium lactate and potassium sorbate 0.5 Percent) significantly increased the total flavonoid content, total soluble solids, flavor index (1.1 Percent) and calcium lactate (1 Percent). Potassium sorbate increased total phenol, total acidity, and fruit acidity significantly compared to other treatments and control (P £ 0.05). Discussion In general, the results of the present study showed that a preharvest application of calcium lactate and potassium sorbate increased firmness, nutritional quality, and antioxidant potential of strawberry fruits. Thus, the use of these two materials is recommended to improve the qualitative and antioxidant properties of strawberry fruit.

The effect of different types of culture substrate on some morphological and physiological indices of African violet (Saintpaulia ionantha H. Wendl)

Masoud Ghasemi Ghahsareh; Foroozan Milani; Saeed Reezi; Masoud Fattahi

Volume 45, Issue 4 , February 2023, , Pages 475-489

https://doi.org/10.22055/ppd.2022.41507.2054

Abstract

Introduction The function of a growth substrate is to provide an ideal medium for plantlet emergence which allows for the optimal development of plants during the time they stay in ... Read More Introduction The function of a growth substrate is to provide an ideal medium for plantlet emergence which allows for the optimal development of plants during the time they stay in the container. A high-water storage capacity is necessary due to the limited volume available in growth substrate. There is a large variety of materials available for substrate elaboration, and their selection depends on the plant species to be propagated, season, propagation system, price, substrate availability and proper features. Compost seems to be an excellent alternative to peat. Also, the fresh material presents a high carbon: nitrogen ratio (C: N) and it may contain substances that are toxic to the plants, such as phenols, resins and tannins. Choosing the suitable planting bed for ornamental plants is one of the problems for most greenhouse managers. In this regard, in order to investigate the possibility of using liquorice residue compost as a by-product of the medicinal plant industry, in growth medium, the effect of peat moss, coir and liquorice root residue compost on the growth of African violet tested in an experiment. Materials and Methods The experiment was done with 7 treatments and 5 replications in a completely randomized design. Experimental treatments included: 60% (volumetric) peat moss, 60% coir, 60% liquorice root residue compost, 30% peat moss + 30% coir, 30% peat moss + 30% liquorice root residue compost, 30% coir + 30% liquorice root residue compost. Each treatment was mixed with 40% perlite. According to the results of a pre-test on the low water retention capacity of liquorice compost, a treatment was also performed using liquorice compost with superabsorbent polymer (Aquasorb made by SNF) at a rate of 5g/kg mixed medium. Results and Discussion Experiment results in African violet showed that in shoot fresh weight, leaf number, root fresh weight, leaf area and relative leaf water content of 30% peat + 30% coir treatment and shoot dry weight, flower number and chlorophyll a were the best in 30% peat moss + 30% liquorice root residue compost treatment. The highest amount of chlorophyll b and total chlorophyll were observed in 60% liquorice root residue compost + superabsorbent polymer, the highest carotenoid content in 60% peat treatment, and the highest root dry weight and proline content in 60% liquorice root residue compost treatment. In general, according to the obtained results, by using licorice root residue compost with peat, it is possible to achieve favorable growth results, and it is recommended to use it up to 50% of the required organic matter in the bed of African violets. Based on the results of this study, licorice root resigue compost with peat moss can achieve favorable growth results and it can be used up to 50% of the required organic matter in African violet bed. The effects of licorice compost on morphological and physiological parameters of plants may be due to its effects on increased water content and soil aeration. Because there is a strong relationship between RWC and plant biomass. That also suggests that plants having greater biomass can maintain higher water content in their leaves. Conclusion Presence of nutrient elements increased the overall fertility, and functionality of the tested media, leading to maintain better plant growth. RWC is a measure of plant water status, which reflects metabolic activity in plant tissues and is one of the most important indicators to identify differences in substrate conditions.

Bioactivity of Methanolic Leaves and Stem Extracts of Adiantum capillus-veneris L. From Southeast of Marivan

Morteza Yazdani; Fereshteh Jookar Kashi

Volume 43, Issue 4 , December 2021, , Pages 487-496

https://doi.org/10.22055/ppd.2020.29309.1757

Abstract

ABSTRACT Introduction:Adiantum capillus-veneris Linn is a medicinal plant belonging to Polypodiales order. Pare-siavashan is the name of Adiantum capillus-veneris Linn in pharmaceutical ... Read More ABSTRACT Introduction:Adiantum capillus-veneris Linn is a medicinal plant belonging to Polypodiales order. Pare-siavashan is the name of Adiantum capillus-veneris Linn in pharmaceutical textbooks of Iranian Traditional Medicine. A. capillus-veneris L. as a medicinal plant was utilized to treat various infectious diseases. In this study, the extract of A. capillus-veneris L. from Kurdistan province is investigated for the first time. Materials and Methods:The plant is collected from southeast of Marivan in October 2017. Theaim of the current study was to investigate cytotoxicity, antimicrobial, antioxidant properties and phytochemical screening of methanol extract and polar and nonpolar subfractions of the leaves and stem of A. capillus-veneris L. separately. The antioxidant activity, total phenolic content (TPC) and total flavonoid content (TFC) of the samples were determined using inhibition of free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH), Folin-Ciocalteu, and aluminum chloride colorimetric methods, respectively. Gentamicin, rifampin and nystatin were used as positive controls in an antimicrobial method. Both 50% lethal concentrations (LC50) and 50% inhibitory concentration (IC50) of the samples were identified using regression analysis. Results and Discussion:Theleaves and stem extractsindicated the highest antioxidative activities in DPPH test that IC50 values =56.23 ± 0.85 and 44.66 ± 1.22 μg/ml respectively, which was higher than the synthetic antioxidant butylated hydroxyl toluene (BHT: IC50= 19 ± 1.03 μg/ml). The total phenolic contents of leaves and stem extracts, as Gallic acid equivalents, were 83.62 ± 1.87 and 147.39 ± 2.35 mg/g, respectively. The amounts of total flavonoids compounds of leaves and stem extracts were also determined 58.50 ± 0.78 and 35.63 ± 0.39 mg, respectively. Alkaloids, triterpenes, flavonoids, saponins, tannins were also identified in all the extracts and glycosides were detected only in water and hexane fraction extract of the stem, also hexaneextraction fraction of stem was devoid of triterpenes and the stem and leaves which contain no tannins. The leaves water extract of A. capillus veneris L. showed maximum zone of inhibition against bactrial strains. The hexane extracts of leaves and stem of A. capillus-veneris L. showed the weakest antimicrobial activity. The brine shrimp lethality bioassay was applied for the isolation of anitumour and cytotoxic agents. The leaves and stem extracts of A. capillus-veneris L. showed significant cytotoxic activity with LC50 values of 125.893 and 97.7237 (μg/ml), respectively. Vincristine sulfate (LC50 0.751 μg/ml) was used as the reference standard of brine shrimp lethality bioassay. Conclusion: This study suggested that the extracts of the A. capillus-veneris L. may be a promising source for novel anticancer agents. The extracts showed moderate to acceptable antibacterial activities.

Yield Stability Analysis of Advanced Spring Wheat Genotypes under Non-Stress and Drought Stress Conditions

Davood daiyoulhagh; Varahram Rashidi; Saeed Aharizad; Farhad Farahvash; Bahram Mershekari

Volume 44, Issue 4 , December 2021, , Pages 489-502

https://doi.org/10.22055/ppd.2020.33143.1889

Abstract

AbstractIntroductionWheat (Triticum aestivum L.) is the first most important cereal crop in the world. Drought, among abiotic stresses, is known as the most complicated, devastating ... Read More AbstractIntroductionWheat (Triticum aestivum L.) is the first most important cereal crop in the world. Drought, among abiotic stresses, is known as the most complicated, devastating factor against wheat production. Although drought impedes wheat yield at all growth stages, its negative impacts are more critical during the flowering and grain-filling phases (terminal drought) and result in substantial yield losses. A prolonged and severe drought will result in a significant decrease in the production of crop and serious issues of food supply and food security. Given the growing population in developing countries such as Iran, and the economic and political importance of producing this cereal, a serious examination at its production and breeding is warranted. Materials and MethodsIn order to study yield stability analysis of advanced spring wheat genotypes under non-stress and drought stress conditions (stop irrigation in the stage of the emergence of 50% inflorescence of each experimental unit), 28 genotypes were evaluated in 2016-2017 and 2017-2018 cropping years in two separate experiments with a randomized complete block design with three replications in the research field of Islamic Azad University of Tabriz. Results and DiscussionCombined analysis of variance under two conditions in two crop years revealed a statistically significant difference between studied genotypes for all traits (P<0.01). Genotype × condition interaction was significant for seed yield, number of seeds in spike, plant height, harvest index in 5% probability level and 1000 seed weight and biomass in 1% probability level. The results of the present study showed that the studied genotypes had different reactions under different environmental conditions for these traits. Based on the results obtained from parametric method which includes environmental variance and environmental coefficient of variation genotypes Karim, Gabos, Zagros, Aftab, URBWYT-94-3, URBWYT-94-9, URBWYT-94-7 and URBWYT-94-2،; in Wricke's Ecovalence genotypes Morvarid, Arta, Gonbad, Roshan, ERWYT-94-8 and URBWYT-94-10; in average rating genotypes Darya, Aftab, ERWYT-94-7, URBWYT-94-10, URBWYT-94-2 and URBWYT-94-4; in standard deviation of rank genotypes Morvarid, Gonbad, Superhead, Arta, Roshan and URBWYT-94-10 known as stable genotypes. Based on non-parametric method of Ketata et al. genotypes Roshan, URBWYT-94-10، ERWYT-94-8 and ERWYT-94-7 known as the stable and high yields genotypes. Overall, the genotypes Karim, Aftab, Roshan, URBWYT-94-7، URBWYT-94-9، URBWYT-94-10 and URBWYT-94-2 introduced as the stable and high yields genotypes. The findings of the study revealed that the results of environmental variance statistics and coefficients of environmental variation are similar, indicating a common trait between them. In other words, the genotypes that were known as sustainable genotypes based on environmental variance stability statistics are exactly those genotypes that were identified as sustainable genotypes based on environmental stability coefficient statistics. ConclusionAs regards the genotypes that were known as sustainable genotypes based on environmental variance stability statistics are exactly those genotypes that were identified as sustainable genotypes based on environmental stability coefficient statistics Therefore, it can be argued that environmental variance stability statistics and environmental stability coefficient statistics are the best indicators in the identification of the stable genotypes in terms of yield.

Improving growth, yield and quality characteristics of greenhouse tomato by using high density fish pond water

Amin Baji; Edris Shabani; Naser Alemzadeh Ansari; Mohamad Albaji

Articles in Press, Accepted Manuscript, Available Online from 11 June 2023

https://doi.org/10.22055/ppd.2023.43551.2098

Abstract

AbstractIntroductionIran ranks 11th in the world in tomato production, with more than 3 million tons produced. Therefore, providing enough water to produce this amount of product will ... Read More AbstractIntroductionIran ranks 11th in the world in tomato production, with more than 3 million tons produced. Therefore, providing enough water to produce this amount of product will be one of the challenges of the near future in the agricultural sector due to the drought problem in Iran. Reuse of fish waste in agriculture is one of the important dimensions of water resources planning. Recycling drainage water from aquaculture farms is one of the necessities because it is rich in substances that can be useful in agriculture. World population and food security is one of the most difficult challenges that most of the countries are facing, because the world population is increasing rapidly and will reach 9.6 billion people by 2050. In this situation, the food supply should increase by 70 to 100%. Considering these challenges, it is necessary to conduct applied research to make maximum use of effluents and wastewaters in the production of greenhouse products.Materials and methodsThe treatments of this experiment included 3 types of nutrient solutions (Resh nutrient solution, fish pond water and modified fish pond water) and 3 promising greenhouse tomato lines (AZ4, AZ5 and V4). The experimental design was factorial arranged in complete block design with 3 replications. At the end of the experiment, the plants were removed from the pot and the roots, leaves and stems were separated from each other. Their fresh and dry weight and the number of leaves were recorded separately for each treatment. To check yield indicators, ripe tomato fruits were harvested during the growth period, counted and weighed with a scale with an accuracy of 0.01 g. The firmness of the fruit tissue was measured by a firmness meter, and the pH and EC of the fruit extract were measured by a pH meter and an EC meter, respectively. The measurement of chlorophyll a, b and total chlorophyll was done using a spectrophotometer in mature leaves developed according to the method suggested by Arnon (1967).Result and DiscussionThe results of this experiment showed that V4 and AZ4 cultivars had the highest stem and leaf weight compared to AZ5 cultivar. Also, the results showed that the Resh solution and modified pond water had the highest root dry weight (58 and 56 g, respectively) compared to the fish pond water treatment (47 g). The findings of this study showed that the use of Resh nutrient solution and modified pool water compared to the treatment of fish pool water increased the number of leaves of tomato plants by 23.88% and 18.69%, respectively. It seems that irrigation with modified pool water and Resh solution increases the growth and development of the plant due to the increase in the level of nutrients. The highest amount of total chlorophyll was observed in the Resh solution and modified pool water with concentrations of 2.3 and 2.2 mg/g, respectively. The results of Silva et al (2021) showed that in irrigation with fish waste, greater availability of magnesium in plant tissue increases the content of chlorophyll b in leaves and increases plant chloroplast pigments. The highest total yield was observed in cultivar V4 and in the treatments of Resh nutrient solution and modified pool water. The results showed that modified pool water had the highest EC of tomato extract (1.9 dS/m). Also, the results of the interaction effect of cultivar and nutrient solution showed that the highest fruit firmness was observed in Resh solution and fish pond water in V4 cultivar. Also, the lowest fruit firmness was observed fish pond water in AZ5 cultivar. It seems that, in the present study, the reduction of calcium absorption in fish pond water caused the reduction of firmness of tomato fruit (Kaab omeir et al., 2020).ConclusionIt seems that according to the above results, it is possible to use pool water with a high density of fish after modification and changes in the amounts of its nutrients in greenhouse tomato cultivation. Among the tested cultivars, the promising line V4 can be recommended for cultivation in the greenhouse for the use of modified fish pond water.

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Effect of Different Concentration of Colchicine on Some Morphological and Phytochemical Characteristics of Dracocephalum Kotschyi Boiss

A.A. Zahedi; B. Hosseini; M. Fattahi

Volume 40, Issue 4 , March 2018, , Pages 31-40

https://doi.org/10.22055/ppd.2017.17279.1322

Abstract

Background and Objectives Dracocephalum kotschyi Boiss. (Labiateae) is an endemic perennial herbaceous plant known in Iran as Zarrin-Giah. Recent pharmacological studies have confirmed ... Read More Background and Objectives Dracocephalum kotschyi Boiss. (Labiateae) is an endemic perennial herbaceous plant known in Iran as Zarrin-Giah. Recent pharmacological studies have confirmed some of the methoxylated flavonoids in plant’s parts having anti-cancer properties. Excessive harvesting of wild plants and limited distribution areas are the main reasons why D. kotschyi is now listed as an endangered plant. Polyploidy induction is an effective tool in medicinal plant breeding. Chromosome duplication and polyploidization may affect plant morphology and breeding systems, ultimately enabling the release of improved genotypes. Materials and Methods In this study, an efﬁcient procedure was established for successful induction of tetraploid D. kotschyi by treating diploid explants with colchicine in horticulture laboratory of Urmia University during 2014-2015 years. Seedlings apical meristem treatment was carried out in two growth phases including, the two and four-leaf plants through presoaking manner. Colchicine at a concentration of 0, 0.05, 0.1, 0.2 and 0.5 % (w/v) was applied in each of these stages. Cytological and morphological evidence conﬁrmed the results of ﬂow cytometry analysis. Results Cytological analyses showed the increase of chromosome numbers from 2n=2x=20 to 2n=4x=40. The results of this study demonstrated for the ﬁrst time that chromosome counting in D. kotschyi of a total of 165 surviving seedlings, 7.27% was found to be tetraploids, 13.3% was chimers while the remainder were diploids. Tetraploid plants demonstrated signiﬁcantly longer stomata and a higher stomatal index compared with diploid control plants. Negative correlation between stomata size, plants height, leaf number and lateral shoot number was obtained in treated plants. The total content of flavonoids increased from 1583.28 in diploids to 1890.07 (μg/g DW) in stable tetraploids. Discussion It seems that 0.5% of colchicine can be used as an effective treatment for polyploidy induction in D. kotschyi.

Evaluating the use of urban treated sewage and nitrogen on yield and forage production of sweet corn

M.J. Fereidooni; H. Farajee; H.R. Owliaie; A. Salehi

Volume 36, Issue 2 , September 2013, , Pages 35-47

Abstract

This study was conducted to evaluate the interaction of urban treated sewage and nitrogen fertilizer on yield and forage production of sweet corn in research farm of Yasouj, during ... Read More

Effect of scarification with sulfuric acid and culture medium type on seed germinatin of strawberry (Fragaria ananassa cv. Camaroasa and Paros) in in vitro conditions

R. i Yosef; M. Mousavi; N. Moalemi; M.H. Ghafarian Mogharab

Volume 36, Issue 3 , December 2013, , Pages 35-44

Abstract

In this study, we investigated strawberry seeds (Fragaria ananassa cv.Camarosa and Paros) germination where they scar using different concentrations of Sulfuric acid (1, 12, 24 and ... Read More

Determination of potassium critical level in date palm leaf and effect of potassium on yield and quality of date fruit (cv sayer)

H. Dialami1

Volume 37, Issue 4 , March 2014, , Pages 35-45

Abstract

In order to determine the potassium critical level in date palm leaf, fertilizer experiments with four treatments from 4 levels of potassium, including: 0, 250, 500 and 750g K from ... Read More

Effects of S, B, Zn, and their interaction on quantitative and qualitative yields of rapeseed as second crop in the paddy field

M. Majidian; T. Shoja; M. Rabiei

Volume 38, Issue 2 , September 2015, , Pages 35-50

https://doi.org/10.22055/ppd.2015.11252

Abstract

In order to the effects of elements zinc, boron and sulfur on quantitative and qualitative agronomic characteristics of rapeseed var. Hyola 401, an experiment was conducted in Rice ... Read More

Effects of Calcium Nitrate on Nitrate Reductase Activity, Amino Acids, Nitrate and Ion Accumulation of Pistacia Vera L., Badami Zarand, Under Sodium Chloride Stress

S. Nasseri Khollari; M. Heydari; S. Jafari; M.H. Daneshvar

Volume 38, Issue 4 , March 2016, , Pages 35-48

https://doi.org/10.22055/ppd.2016.11833

Abstract

Nitrogen is considered as an essential nutrient for plants and could be taken up in large amounts by plants. Nitrogen fertilizers are known to have beneficial effects when added to ... Read More

Variation in Root and Shoot Growth, Rhizobium Nodules of Fenugreek (Trigonella foenum gracum) Under Fertilizer Treatments and Intercropping with Isabgol (Plantago ovate)

S. Ghasemi Maham; S. Fallah2; M.R. Tadayyon

Volume 39, Issue 1 , June 2016, , Pages 35-46

https://doi.org/10.22055/ppd.2016.12040

Abstract

Background and Objectives The use of organic manures and legume species can reduce the need for fertilizers and sustain cropping systems. Organic manure plays a vital role in bringing ... Read More Background and Objectives The use of organic manures and legume species can reduce the need for fertilizers and sustain cropping systems. Organic manure plays a vital role in bringing stability and sustainability to agriculture and also avoiding over dependence on chemical fertilizers. Nowadays, consumer preference is more for organically grown produce because they are free from toxic residues and have concern for the environment. Animal manure is a good source for organic fertilizer as it helps to improve chemical, physical and biological properties of soil and it is used as a source of energy for the soil ecosystem. The aim of this study was investigating combination of animal manure and inorganic fertilizer on growth and yield performance of fenugreek (Trigonella foenum gracum). Materials and methods In order to evaluate fertilizer treatments and intercropping ratio of isabgol-fenugreek on rhizobium nodules, root and shoot growth, an experiment was conducted at Agricultural Research Farm, Shahrekord University in 2013. The experiment was laid out in a randomized complete block design (RCBD) with seven treatments. Four intercropping treatments consist of sole cropping of fenugreek (T), three ratios of intercropping of isabgol-fenugreek (1:1, 1:2, 2:1) as the first factor, and three fertilizer treatments (manure, manure: chemical (50:50), and chemical fertilizer) were investigated in factorial as randomized complete block design with three replications. In this experiment, parameters such as rate of weight and number of rhizobium nodule per plant, dry weight of root, dry weight of stem, dry weight of leaf and dry weight of pod, number of lateral branches, leaf number, leaf area index, plant height, special leaf area, leaf area ratio, leaf weight ratio and total weight of shoot were measured. Results The results showed that the maximum number and weight of rhizobium nodules, shoot dry weight, number of lateral branches were obtained in isabgol-fenugreek (2:1) with the application of combined fertilizer. The maximum of isabgol height and pod weight were achieved in combined fertilizer. Also, the lowest number and weight of rhizobium nodules, specific leaf area, leaf area ratio, and leaf weight ratio were produced in the chemical fertilizer. Means comparison results indicated that in TP (2:1) with application of combined fertilizer number (16.4), weight (0.28 g) of rhizobium nodules, shoot dry weight (6.82 g), number of lateral branches (4.93) and leaf area index (2.2) were obtained in isabgol-fenugreek (1:2) with application of combined fertilizer. discussion In general, combined use of fertilizers in interaction with intercropping isabgol-fenugreek can be effective in increasing the fenugreek performance and environment conservation by reducing of chemical fertilizers.

Evaluation the genetic diversity of 10 Milk thistle (Silybum marianum L.) ecotypes using morphological, phenological and phytochemical traits

R. Hamid; M.R. Siahpoosh; R. Mamaghani; A. Siahpoosh

Volume 37, Issue 1 , June 2014, , Pages 37-47

Abstract

Understanding milk thistle genetic diversity is essential for breeding programs of this plant. In order to investigate genetic diversity of some Iranian ecotypes, an experiment was ... Read More

Influence of three species of mycorrhizal fungi (Glomus SPP.) on physiological characters of wheat under the salinity conditions

S. Habibi; M. Meskarbashee; M. Farzaneh

Volume 37, Issue 3 , December 2014, , Pages 37-52

Abstract

Effect of Seed Deterioration on Physiological and Biochemical Traits of Oil Flax (Linum usitatissimum L. Norman var.) Seed

H. Balouchi; R. Ostadian Bidgoly

Volume 40, Issue 2 , September 2017, , Pages 37-52

https://doi.org/10.22055/ppd.2017.13116

Abstract

Background and Objectives Seed deterioration is a natural phenomenon that occurs in all seeds and leads to gradual decline of seed viability during storage. However, the rate of seeds ... Read More Background and Objectives Seed deterioration is a natural phenomenon that occurs in all seeds and leads to gradual decline of seed viability during storage. However, the rate of seeds age depends upon their physiological status, genetic constitution and storage conditions. The lipid peroxidation through the production of free radical plays an important role in the loss of seed viability during seed storage. Material and Methods In order to investigate the effect of deterioration on germination indices and enzymes activity of the flax oil seed, a factorial experiment was conducted based on a Completely Randomized Design with four replications in the seed laboratory of Yasouj University in 2015. The factors included 4 levels of temperature (15, 25, 35 and 45oC) and moisture content (5, 9, 13 and 17%). With relation intended seed moisture, Hampton and Teckrony (1995) was calculated. After determining the moisture content of the seeds in envelopes of aluminum foil was placed, then the amount of water the need added and to ensure that the packaging and moisture exchange with the outside world for 24 hours at 15 °C were identical to the seed moisture and then for 6 months in storage conditions at temperatures of 15, 25, 35 and 45oC were kept. Results Analysis of variance showed that the single effects including temperature and seed moisture content experimental treatments for traits including germination percentage, germination rate, seedling vigor index (weight), activity enzymes antioxidant catalase, superoxide dismutase and ascorbate peroxidase, soluble proteins, electrical conductivity and oil percentage were significant (P≤0.01). Also, the interaction temperature and seed moisture content of all treatments except for enzyme, activity of superoxide dismutase and ascorbate peroxidase were significant (P≤0.01) but were significant (P≤0.05) for the activity of superoxide dismutase and ascorbate peroxidase. The results showed that with increasing temperature and moisture content, all the physiological and biochemical traits, except for electrical conductivity, decreased. The decrease in germination was also associated with a decrease in the activity of antioxidant catalase, superoxide dismutase and ascorbate peroxidase and as a result the antioxidant system was not sufficient to protect seeds against free radical damage. With the increase in reactive oxygen species, lipids peroxidation increased, probably due to the destruction of cell membranes, increased electrical conductivity seed the negative correlation between the electrical conductivity and the activity of the enzymes antioxidant indicator of the issue. Discussions Thus, oil flax seed deterioration was closely related to decrease in the activities of free radical detoxifying enzymes and increased lipid peroxidation. In general, the best flax seed storage condition is at 15°C and 5 percent moisture content.

Effects of Drought Stress on Enzymatic and Non- Enzymatic Antioxidants in Flag Leaf and Spikes of Tolerant and Sensitive Wheat Genotypes

P. Sharifi; N. Mohammadkhani

Volume 41, Issue 3 , December 2018, , Pages 37-50

https://doi.org/10.22055/ppd.2018.20256.1420

Abstract

Abstract Background and Objectives Drought stress is one of the main limiting factors in plant production in semiarid regions; this is due to its oxidative stress. Materials and Methods ... Read More

Karyotypic Analysis of Garlic (Allium sativum) Accessions in the North of Iran

Seyyed Javad Mousavizadeh; Mostafa Khoshhal Sarmast; Kambiz Mashayekhi

Volume 44, Issue 1 , June 2021, , Pages 37-50

https://doi.org/10.22055/ppd.2019.27338.1661

Abstract

AbstractBackground and ObjectivesAllium sativum is one of the most important, mostly used economical vegetable plants in Iran. Chromosome analysis or karyotyping is a test that evaluates ... Read More AbstractBackground and ObjectivesAllium sativum is one of the most important, mostly used economical vegetable plants in Iran. Chromosome analysis or karyotyping is a test that evaluates the number and structure of chromosomes to study evolution. The present study was carried out to explore the cytological features, including chromosome number and karyotypic characteristics in a local cultivar of Allium sativum L., north of Iran (Gorgan, Sari, and Rasht accessions). Materials and MethodsThe root tip cells were treated first with 0.002 mol·L−1 8-hydroxyquinoline for 4 h at 4°C and then fixed for 24 h in fixative (1:3 glacial acetic acid/absolute alcohol) at 4°C. Finally, the root tips were softened with 1 M hydrochloric acid at room temperature for 8 min and stained for 10 min with acceto orcein 2% before being squashed between a glass slide and a coverslip. Root tip cells photographed at mitosis metaphase, which had well-separated chromosomes and a clear centromere. Long arm, short arm, the ratio of long arm to short arm, and chromosome index of mitotic chromosomes were calculated from metaphase plates. Figures summarized the idiogram for the three garlic accessions, based on the eight pairs of chromosomes’ short arm order length. ResultsBasic chromosome number was confirmed as x = 8 (2n = 2x = 16). All the studied accessions cytotypes were diploid. The largest chromosome length belonged to Gorgan accesion as amount 169.51 micrometers, and the smallest chromosome length was obtained in Rasht accesion as amount 136.57 micrometers. The long arm of the chromosome was recorded between 4.85 to 6 micrometers. The short arm of the chromosome was measured between 3.18 to 3.80 micrometers. The Metaphase karyotype analysis revealed that centromeres were mainly located at the middle (metacentric - m) or near the middle (submetacentric - sm) of chromosomes. The chromosomes are arranged in decreasing order for short arm length, and the karyotype formula was obtained 12m+4sm for all accessions. Chromosomes 13 to 16 were submetacentric for both Gorgan and Rasht accessions. Submetacenteric Chromosomes were observed on chromosomes number 11-12 and 15-16 for Sari accesion. Three of the studied accessions were evolutionary located in one level according to asymmetrical karyotype, the value of TF% and separating to 2B classes based on Stebbins categories. DiscussionRegarding asymmetric karyotype and according to interchromosomal asymmetry (A2) as well as difference between the range of relative length (DRL), the most were found in Rasht accession (A2=0.34; DRL=6.99). A2 and DRL are two aspects of karyotype asymmetry related to shifts of the centromere position from the median to terminal or subterminal. The karyological data provide a comprehensive cytogenetic resource useful in cytotaxonomic research and assessment of their role in the evolution of Garlic. This resource can help better understand the taxonomy, evolution, and speciation in the genus Allium and identify candidate species for breeding programs.

Determination of critical period of weed control in corn (Zea mays L.) in Ahvaz region

A. Gerami; S.A. Siadat; A.M. Bakhshandeh; Ghodratallah Fathi; K. Alami Saeid

Volume 36, Issue 1 , June 2013, , Pages 39-49

Abstract

In order to determine the critical period of weed control in grain corn (Zea mays L.), a field study was conducted at the Ramin University ofAhvaz Research Field in 2009. The experiment ... Read More

The Effects of Different Amounts of Natural Zeolite on Vegetative and Reproductive Characteristics of Narcissus tazetta L. cv. Shahla

F. Nazari; H. Farahmand; M. Ghasemi Ghehsareh3

Volume 37, Issue 2 , September 2014, , Pages 39-48

Abstract

There exist a lot of reports about the positive and significant effect of natural zeolite on vegetative and reproductive characteristics of plants such as cereals, vegetable transplants, ... Read More

Characterization of Pear Tree Varieties in West and Center of Kurdistan

A.K M

Volume 32, Issue 1 , September 2009, , Pages 39-51

Abstract

According to some observations, west of Iran is part of pear origin center. Topographical and geographical situations of Kurdistan province lead to plants variation. There are different ... Read More

Effect of Temperature Change on Stomatal Conductance and Chlorophyll Content of Wheat

H R; M N; F M; M M

Volume 34, Issue 2 , February 2012, , Pages 39-52

Abstract

Stomata have a sensitive role in plant physiology aspect investigation, specially under stress conditions. This experiment was carried out in Ahvaz Shahid Chamran University Research ... Read More Stomata have a sensitive role in plant physiology aspect investigation, specially under stress conditions. This experiment was carried out in Ahvaz Shahid Chamran University Research Field in 2005 and 2006 under randomized complete block design with 4 replications. The main plots included three planting date (6 November, 26 November and 16 December) and the subplot were three wheat cultivar (Fong, Chamran and Star). The results showed that the deterrence of the aweraye of flag leaf water potential under planting date treatment was significant (1% level), but flag leaf water potential under different cultivars and the interaction between planting date and cultivars was not significant. RWC (relative water content) was different during grain filling and in four sampling in difference planting date and in cultivars for many sampling observed significant difference but the difference was not significant in cultivars interaction and planting date. Stomata conductance in different grain filling stages in planting date showed significant difference. Also under cultivars, there was a significant difference (1% level) but their interaction was not significant. The most stomata resistance was in third planting date and mean comparison of this character in cultivars showed that near the end of grain filling. The highest and lowest stomata conductance was in Fong and Chamran cultivars, 0.93 and 0.67 cm/s respectively. Planting date interaction and cultivar showed that the first sampling exception, in other grain filling period SPAD number influenced by them and in fourth sampling (28 days after flowering) the first and third planting date allocated 41.34 SPAD number to themselves respectively. Grain filling period coincident with heat stress in near the end of season, induced that effective parameters in yield (ψp, RWC, gs and chlorophyll density) located in undesirable conditions. Changing the planting date (late planting date) which caused coincident grain filling to more heat stress and made the sever effective on grain weight compare to biomass as effective of planting date on grain (1% level) had significant different and maximum seed single dry weight was 45.16 and 36.39 mgr in the first and the third planting date respectively. Also, early Fong cultivar with better use from environmental condition got high yield compared to other two cultivars.

Volume 46 (2023)

Volume 45 (2022)

Volume 44 (2021)

Volume 43 (2020)

Volume 42 (2019)

Volume 41 (2018)

Volume 40 (2017)

Volume 39 (2016)

Volume 38 (2015)

Volume 37 (2014)

Volume 36 (2013)

Volume 35 (2012)

Volume 34 (2011)

Volume 33 (2010)

Volume 32 (2009)

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