Plant Productions - Articles List

Research Paper - Agroecology

Study of Soybean (Glycine max (L) Merrill) and Chia (Salvia hispanica L.) Competition in the Different Intercropping Ratios Based on Replacement Method

Rahmat Abbasi; Meisam Namdari

Volume 45, Issue 1 , May 2022, Pages 1-14

https://doi.org/10.22055/ppd.2022.37720.1986

Abstract

IntroductionToday, efficient agricultural systems such as intercropping are essential due to the optimal use of resources. Meanwhile, using the Legume plant in intercropping can increase ... Read More IntroductionToday, efficient agricultural systems such as intercropping are essential due to the optimal use of resources. Meanwhile, using the Legume plant in intercropping can increase the efficiency of biological nitrogen fixation and be a sustainable approach to meet the crop's nitrogen needs. Since the planting pattern and component crop greatly influence this biological process, selecting complementary species in intercropping can play an essential role in increasing flexibility and sustainability in farming systems. Materials and MethodsIn order to study the effects of intercropping culture on the agronomic traits of Chia and Soybean, a field experiment was conducted in a randomized complete block design with four replications at the research farm of Sari Agricultural Science and Natural Resources University in 2019. The planting ratios were 0:100, 25:75, 50:50, 75:25, and 100:0 (Soybean: Chia respectively) using replacement method. Results and DiscussionResults showed that intercropping significantly affected shoot height, No. branch, grain yield, components yield, and the N. derived from the atmosphere (Ndfa). In the Chia, reducing the number of planting rows in different planting ratios, shoot height, and 1000-seed weight increased in contrast to the number of inflorescences per plant. Different planting ratios had no significant effect on the number of branches per plant of Chia. The soybean crop had the highest no. of branches, the number of pods per plant, and the 1000 seed weight in the 25:75 planting ratio. The highest shoot height was related to the monoculture soybean (100:0), and with decreasing the number of soybean planting rows in intercropping, the shoot height decreased. Also, the Ndfa in intercropping culture was higher than in monoculture during different growth stages. Its amount increased with a decreasing share of soybean planting in intercropping. Biological nitrogen fixation in soybean intercropping cultivation with Chia increased 75 days after planting and then decreased. Besides, the pure stand of Soybean (100: 0) and Chia (0: 100) had the highest and lowest grain yield with an average of 4629.57 and 823.14 kg. ha-1, respectively. Considering the total yield of Chia and Soybean in intercropping cultivation and the compensatory ability to increase the yield of Soybean against the decrease in the yield of Chia, the effect of competition between the two crops is positive complementation. lculation of land equivalent ratio (LER) revealed that the planting ratio of 25:75 had the highest efficiency by 22 percent. ConclusionChia in intercropping can improve the yield components of Soybean while improving the efficiency of biological nitrogen fixation. Increasing the efficiency of intercropping cultivation in the planting ratio of 25:75 due to the increase of 75.37% is the share of the complementarity effect on grain yield, creating balance and facilitating inter-species competition.

Research Paper - Horticulture

Correlation between Yield and Qualitative Traits of Sugar Beet Cultivars with Rhizomania Disease Indices under Field Infection Conditions

Masoud Ahmadi; Hassan Hamidi; Jamshid Soltani Idliki; Javad Rezaei; Mozhdeh Kakueinejad

Volume 45, Issue 1 , May 2022, Pages 15-28

https://doi.org/10.22055/ppd.2020.32377.1873

Abstract

IntroductionRhizomania viral disease is one of the limiting factors of sugar beet cultivation and significantly reduces its yield. The most important way to deal with this disease is ... Read More IntroductionRhizomania viral disease is one of the limiting factors of sugar beet cultivation and significantly reduces its yield. The most important way to deal with this disease is to use resistant cultivars. Materials and MethodsIn order to evaluate the correlation between yield and qualitative traits of sugar beet cultivars with rhizomania disease indices, an experiment was conducted in a randomized complete block design with 12 different sugar beet cultivars in four replications in test site with natural infection with rhizomania in Torbat-e-Jam area at two years (2017 and 2018). The traits studied in this study included root yield, sugar content, sugar yield, white sugar content, white sugar yield, sodium, potassium, trace nitrogen, alkalinity, sugar extraction coefficient, leaf growth score, root growth score, disease severity and ELISA test index (OD). Analysis of variance was performed using SAS v.9.1 statistical software. Mean comparisons of traits were performed using LSD test at the probability level of 5 and 1%. Results and DiscussionAnalysis of variance showed significant differences among genotypes for all studied traits except for sodium. Cluster analysis of yield, crop quality as well as rhizomania severity of infection divided the sugar beet cultivars into three groups (resistant, semi-resistant and sensitive). Correlation between traits in a rhizomania infected field and different levels of resistance to rhizomania was different. In all cultivars with different levels of resistance, there was a significant correlation between pure sugar yield with leaf growth score (r=0.88 **), root growth score (r=0.85**), disease severity (r= -0.84 **) and ELISA index (r= -0.67 **) was observed. Therefore, these traits are suitable indicators for evaluation of cultivars for suseptibility or resistance to rhizomania disease. In addition, correlation between ELISA index with disease severity (r=0.83**), leaf growth score (r= -0.61 **) and root growth score (r= -0.67 **) was significant. ConclusionAccording to the obtained results, because the ELISA test and determining the virus concentration is expensive, and on the other hand, this index had a positive and very significant correlation with the severity of the disease infection, so the disease severity index can be used to evaluate cultivars for resistance to rhizomania disease.

Research Paper - Horticulture

Effect of Methanol Spraying on some Morphophysiological Characteristics, Yield and Yield Components of Sunflower (Helianthus annuus L.) under Drought Stress Conditions

Hamed Javadi; Seyyed Gholam Reza Moosavi; Mohammad Javad Seghatoleslami; Ali Reza Ebrahimi; Mohammad Kozegar

Volume 45, Issue 1 , May 2022, Pages 29-40

https://doi.org/10.22055/ppd.2020.33127.1888

Abstract

IntroductionSunflower (Helianthus annuus L.) is one of the five important oil plants of Iran. Water deficit stress is one of the most important limiting factors in sunflower cultivation. ... Read More IntroductionSunflower (Helianthus annuus L.) is one of the five important oil plants of Iran. Water deficit stress is one of the most important limiting factors in sunflower cultivation. Methanol is used to deal with environmental stresses, especially dehydration. Preventing or reducing light respiration, delaying leaf senescence, increasing photosynthetic activation period and leaf area duration index and increasing CO2 fixation are among the roles of methanol application in plants. Therefore, this study was conducted to investigate the effect of methanol spraying on some morphophysiological characteristics, yield and yield components of sunflower under drought stress conditions. Materials and MethodsThis field experiment was conducted as a split plot based on randomized complete block design with three replications in Birjand, Iran, in 2017. The main plots included two irrigation levels (irrigation after 80 and 160 mm evaporation form class A pan). On the other hand, the sub- plots included five levels of methanol spraying; control (spraying with water), 7, 14 and 21% v/v. Statistical analysis was performed using MSTATC software. Duncan's multiple range test at the 5% level of probability was used to compare the means. Results and DiscussionResults showed that deficit irrigation reduced stem diameter, number of grains per head and kernel/husk weight ratio. Methanol spraying also increased the number of grains per head. Also, methanol spraying improved most of the studied traits under two irrigation treatments. So that under drought stress conditions, foliar application with 28% volumetric methanol increased plant height (9.9%), stem diameter (5%) and leaf number per plant (14.7%). Compared with control, foliar application with 14% and 21% volumetric methanol increased SPAD by 8% and 34.7%, respectively and also, increased 1000-grain weight (12%), grain yield (27.9%) and biological yield (23.4%). According to the results, under optimum irrigation conditions, maximum sunflower grain yield was obtained with 28% volumetric methanol spraying, but under drought stress conditions, spraying with 21% volume methanol was the best one. ConclusionThe results showed that foliar application of methanol under optimum and also deficit irrigation conditions did not affect the percentage and yield of sunflower oil seed, but increased grain yield. Maximum grain yield under optimum irrigation was obtained with 28% volumetric spraying, which was 27% more than control. Also, the results showed that under arid and semi-arid conditions of Birjand, deficit irrigation with using methanol (21% volumetric) produces acceptable grain yield.

Research Paper - Horticulture

The Effect of Volck Mineral Oil Spraying on Flowering and Biochemical Characteristics of Two Olive Cultivars under Ahvaz Condition

Sara Davoodi; Shohreh Zivdar; Esmaeil Khaleghi

Volume 45, Issue 1 , May 2022, Pages 41-52

https://doi.org/10.22055/ppd.2021.34536.1933

Abstract

IntroductionOlive (Olea europaea L.) is an evergreen tree and one of the most important products of the horticultural section in Iran due to high nutritional value of its oil and fruits. ... Read More IntroductionOlive (Olea europaea L.) is an evergreen tree and one of the most important products of the horticultural section in Iran due to high nutritional value of its oil and fruits. Adaptation of olive cultivars to the climatic conditions of different regions are not the same. The results of studies showed that special cultivars are recommended for each region. In tropical and subtropical climates with mild winter such as Ahvaz, the chilling requirement of olive trees is not completed. This condition has led to poor and insufficient flowering in some olive cultivars. Mineral oils, including volck oil are one of the most beneficial treatments to complete chilling requirement and bud break in fruit trees. The aim of this study was to investigate the response of two olive cultivars (Manzanilla and khaziri) to the application of volck mineral oil and studying their flowering and biochemical characteristics in Ahvaz condition. Materials and MethodsIn this experiment, the effect of volck mineral oil spraying [control (water spray), 1, 3, 5 %] on two olive cultivars were investigated. The experimental design was split plot arranged in complete block design with four replications. Measured traits included flowering characteristics such as the number of inflorescences in the branch, flowers in inflorescence, complete flowers, flowering days after treatments, percentage of flower fall, fresh and dry weight of flower and inflorescence. Biochemical properties such as bud carbohydrates and ABA, leaf proline and chlorophyll were measured. Results and DiscussionThe results showed that foliar application of 3 and 5 % volck oil caused flowering (31.8 and 28.7 days after treatment, respectively) in Manzanilla cultivar. Bud carbohydrate content increased significantly after tree spraying with volck oil in all of three concentrations. The highest content of bud carbohydrates (78.68 mg/g) was obtained in khaziri when treated with 3% volck oil. The use of volck oil was effective in increasing the ABA concentration in Manzanilla cultivar and its effect was as 3 and 5% volck oil (83.1 and 105.4 µg/g, respectively). The amount of proline in both cultivars increased until 60 days after applying three concentrations of volck oil. The levels of chlorophyll a, chlorophyll b and total chlorophyll increased until 30 days after treatment and then were decreased. Volck oil (3%) can be used for flowering of Manzanilla cultivar in climatic conditions of Ahvaz. Based on the obtained results, it seems that the effect of volck oil on flowering and biochemical traits depends on the type and concentration of mineral oil and type of the cultivar. In the present study, flowering occurred when Manzanilla cultivar treated with mineral volck oil (3 and 5%) and the flowering traits were improved at 3% oil more than other treatments. ConclusionAccording to the obtained results, olive cultivars show different reactions to the use of Volck oil, and Volck oil with a concentration of 3% can be used for the flowering of Manzanilla cultivar in the weather conditions of Ahvaz.

Research Paper - Horticulture

Optimization of in vitro Cultural Conditions of Haworthia (Haworthia Cooperi Baker)

Mahdiyeh Kharrazi; Maryam Moradian; Nasim Safari; Azadeh Khadem; Ahmad Sharifi

Volume 45, Issue 1 , May 2022, Pages 53-66

https://doi.org/10.22055/ppd.2021.36744.1971

Abstract

IntroductionHaworthia cooperi is a type of ornamental succulent that is propagated by separating the offsets and leaf cuttings. Due to the small number of produced offsets by the mother ... Read More IntroductionHaworthia cooperi is a type of ornamental succulent that is propagated by separating the offsets and leaf cuttings. Due to the small number of produced offsets by the mother plant, the rate of propagation in this way is low, so micropropagation can be considered as an effective and efficient way to propagate this ornamental plant. Materials and MethodsFor this purpose, in the present study, the growth response of Haworthia cooperi plants to in vitro culture conditions in 1398 in the research laboratory of the Department of Ornamental Plants Biotechnology was investigated. This research was conducted in the form of three separate experiments based on a completely randomized design with three replications. In the first experiment, the effects of cytokinin type (BA and KIN) and concentration of cytokinin (0, 1, 2 and 3 mg/l) on leaf explant regeneration were evaluated. In the second experiment, the effects of culture medium type (MS and B5) and concentration of culture medium (0.5 and 1) on plantlet propagation were investigated. In the third experiment, plantlet rooting rate was evaluated under the influence of auxin type (NAA, IBA and IAA) and concentration (0, 0.5, 1 and 1.5 mg/l) of auxin. Results and DiscussionThe results showed that application of BA and KIN hormones increased the number of regenerated plantlets. The highest number of plantlets (5.50) was recorded in the MS medium containing 3 mg/l BA with 0.5 mg/l NAA. Plantlets cultured in the hormone-free culture medium had no regenerated symptoms. The number of plantlets increased with increasing the concentration of both hormones, but BA hormone in the culture medium had a greater effect on this factor. Lack of cytokinin hormone application in culture medium led to root production; however, the use of BA and KIN hormones in the culture medium reduced the number of produced roots by plantlets. The highest number of roots was recorded in hormone-free culture medium and the lowest number was recorded in the high concentration of BA hormone. The reduction in the number of roots in the culture medium containing BA was greater than the culture medium containing KIN. The of the second experiment revealed that use of MS culture medium showed better performance compared to B5 culture medium. Thus, the highest dry weight of plantlet and roots was obtained at full concentration of MS culture medium. The results of rooting stage showed that the presence of NAA hormone in the culture medium increased rooting in the plant. However, the produced roots in this culture medium were so thick that these roots are not suitable for plant growth in the acclimation stage. Therefore, considering the number and length of produced roots, the optimal culture medium for plantlet rooting is ½ MS culture medium containing 1 mg/l IAA hormone. Determining the best type of culture medium and optimizing it for regeneration, proliferation and rooting is one of the important factors in success in vitro culture conditions. Based on the results of the current study, application of MS medium containing 3 mg/l BA with 0.5 mg/l NAA was so effective for regeneration phase of this ornamental plant. Also, the efficiency of MS culture medium in the process of propagation of this plant is most likely related to the suitability of the components of this culture medium. In general, due to the presence of plant growth regulators such as NAA, IBA and IAA in the culture medium, rooting is induced in plantlets. In the present study, the use of IAA hormone at a concentration of 1 mg/l was a suitable hormonal combination for rooting in this ornamental plant. ConclusionBased on the results of the present study, the application of MS culture medium containing 3 mg/l BA with 0.5 mg/l NAA is recommended for regeneration and proliferation of tissue culture plantlets of Haworthia cooperi. It is also recommended to use ½ MS culture medium containing 1 mg/l of IAA hormone for rooting of tissue culture plantlets.

Research Paper - Horticulture

Effect of Biochar and Hydrogel on Morphophysiological and Biochemical Characteristics of Common Sage (Salvia officinalis L.) under Drought Stress

Shayesteh Ghias; Mostafa Shirmardi; Heidar Meftahizadeh; Maryam Dehestani Ardakani

Volume 45, Issue 1 , May 2022, Pages 67-80

https://doi.org/10.22055/ppd.2021.36030.1962

Abstract

IntroductionDrought and low organic carbon content are two main problems for agriculture in arid and semiarid regions. Drought stress is responsible for at least 40% of crop losses ... Read More IntroductionDrought and low organic carbon content are two main problems for agriculture in arid and semiarid regions. Drought stress is responsible for at least 40% of crop losses in the world. The use of biochar (BC) and hydrogels can decrease adverse effects of drought on the plant. The objective of this study was to evaluate the effect of BC and hydrogel application on morphophysiological and biochemical parameters of Salvia officinalis L. under drought stress (DS). Materials and MethodsA factorial experiment with a randomized complete block design with three replicates was conducted in the research greenhouse of the Meybod industrial town in 1398/2018 to investigate the BC and hydrogel effects on the growth, physiological and biochemical characteristics of Salvia officinalis L. under drought stress condition. The BC and hydrogel was applied by mixing dry soil at rates of 0, 10, and 20 ton BC.ha-1, 100, and 200 kg hydrogel.ha-1 with three DS levels of D1 (irrigation in 80% field capacity (FC) as no-stress conditions), D2 (irrigation in 60% FC as mild-stress conditions) and D3 (irrigation in 40% FC as severe-stress conditions). The growth responses were examined included morpho-physiological (i.e., chlorophyll content, leaf area, relative water content (RWC), ion leakage (IL), and phytochemical (i.e., antioxidant activity (AA) and total phenolic content (TPC)) parameters. Results and DiscussionResults showed that drought stress significantly decreased growth parameters and RWC while increased IL, AA and TPC. Application of 200 kg hydrogel.ha-1 and 20 ton BC.ha-1 significantly increased chlorophyll index, root and shoot dry weight compared to the control under severe stress condition. At mild stress, all treatments significantly reduced the root:shoot ratio compared to the control. However, in severe stress, only application of 10 and 20 ton BC.ha-1 significantly reduced this parameter compared to the control. In no stress and sever stress, use of 200 kg hydrogel.ha-1 increased RWC in plant in comparison with control (15.9 and 5.1% respectively). Results indicated that 200 kg hydrogel.ha-1 and 20 ton BC.ha-1 treatments significantly decreased IL and TPC in mild and sever stress conditions in comparison with control. All treatments significantly decreased AA in severe stress and control has the highest amount of AA in this level of drought. It seems that use of BC and hydrogel in soil increased soil water holding capacity and improve soil structure. In addition, BC contains various elements that can improve plant nutrition under drought stress. The amount of BC and hydrogel application is very important on the effectiveness of these amendments on the plant characteristics under stress conditions. ConclusionThe application of hydrogel and biochar could improve the growth indices, leaf relative water content and reduce the total phenol concentration and antioxidant capacity in the plant under drought stress. It seems that these amendments reduce the negative effect of drought stress on the plant by increasing the soil water holding capacity, improving the structure and increasing the availability of nutrients for the plant.

Research Paper - Horticulture

The Effect of Two Mycorrhiza Fungi Species on Biochemical Characteristic and Aerial Dry Biomass of Coriander (Coriandrum sativum L.) Under Cadmium Stress

Fariba Mohammadifard; Mohammad Moghaddam

Volume 45, Issue 1 , May 2022, Pages 81-94

https://doi.org/10.22055/ppd.2020.30809.1817

Abstract

IntroductionNowadays, soil pollution to various heavy metals is one of the most important environmental issues in human societies, which has adverse effects on soil and soil flora and ... Read More IntroductionNowadays, soil pollution to various heavy metals is one of the most important environmental issues in human societies, which has adverse effects on soil and soil flora and groundwater contamination. Among the heavy metals, cadmium with a half-life of about 20 years, its high mobility in the soil and absorption by the plant, has a significant toxicity that its presence in the food chain seriously threatens human health. A wide range of studies have shown that inoculation with mycorrhizal fungi increases the resistance of many plants against heavy metals. Materials and MethodsIn order to investigate the effect of mycorrhizal fungi on biochemical properties of coriander under heavy metal stress, a pot experiment was conducted in the greenhouses at Ferdowsi University of Mashhad. A factorial experiment based on completely randomized design with 2 factors and 3 replications was conducted. The first factor was heavy metal in 4 levels of 0, 20, 40, 80 mg/kg nitrate cadmium soil and the second factor was mycorrhiza fungi in 3 levels without fungi and Glomus mosseae and Glomus intraradicese application. The soil mixture included soil, leafy soil and sand in a ratio of 1: 1: 1. All traits were measured at flowering stage. The studied traits include aerial parts dry biomass, chlorophyll, b, a, electrolyte leakage, soluble carbohydrate, antioxidant activity, total phenol, total flavonoids and proline. Data analysis using Minitab 17 software was done. Results and DiscussionThe results were showed that by increasing the concentration of cadmium, the amount of electrolyte leakage, soluble carbohydrate, antioxidant activity, total phenol, total flavonoids and proline increased. However, with increasing cadmium concentration, the amount of dry biomass of aerial parts, chlorophyll, a, b and carotenoids decreased. So, with increasing cadmium concentration, the amount of aerial parts dry biomass of coriander decreased by 40% compared with the control. Based on this study, using of mycorrhizal fungi under cadmium pollution reduced the stress influence, so that the highest amount of aerial parts dry biomass was found in plants treated with mycorrhizal and without cadmium application. The lowest amount dry biomass of aerial parts was found in without mycorrhizal with highest nitrate cadmium concentration (80 mg/kg soil). The highest amount of proline, soluble carbohydrate, phenol, antioxidant activity, and total flavonoids was observed in plants which treated with the highest nitrate cadmium concentration (80 mg/kg soil) and Glomus intraradicese application. ConclusionSo that inoculated plants with mycorrhizal fungi had drier biomass of aerial parts than plants without mycorrhiza. According to the results of this study, in the conditions of environmental stresses such as cadmium stress, the use of mycorrhizal fungi reduces its destructive effects, which can be used as a management solution in heavy metal contaminated areas. In general, the results of this study showed that application of Glomus mosseae fungus in cadmium stress has a better effect on the traits and its application is recommended in this condition.

Research Paper - Agronomy

Effect of Waterlogging Stress on Yield and Yield Components and Photosynthetic Characteristics of Two Mung Bean Cultivars in Ahvaz Conditions

Seyed Bashir Fazeli; Afrasyab Rahnama; Payman Hassibi

Volume 45, Issue 1 , May 2022, Pages 95-108

https://doi.org/10.22055/ppd.2020.30538.1805

Abstract

IntroductionMung bean (Vigna radiata L.) is one of the most important legumes in the world. This nitrogen-stabilizing plant has rapid growth and a short growth period, leading to improved ... Read More IntroductionMung bean (Vigna radiata L.) is one of the most important legumes in the world. This nitrogen-stabilizing plant has rapid growth and a short growth period, leading to improved soil fertility. Mung bean is widely cultivated in tropical and subtropical regions of Asia due to its high adaptation range. Plants are obligate aerobic organisms and thus depend on O2 to grow and compete successfully with neighbouring plants in natural environments. Waterlogging stress is one of the most important factors limiting the planting of mung bean under Ahvaz conditions. Materials and MethodsIn order to study the effects of waterlogging stress on some agronomic and physiological characteristics of two Mung bean cultivars, a pot experiment was conducted as split factorial in a completely randomized block design with three replications in the field. The main plots were different growth stages consisted of vegetative growth stage, the beginning of flowering, grain filling stage, and three growth stages. The combination of stress levels (control and waterlogging stress) and two Mung bean cultivars (Hindi and Omrani) were as subplots. Results and DiscussionWaterlogging was caused to decrease significantly dry matter and grain yield in both cultivars at each growth stages, In Hindi cultivar, the highest grain yield reduction was observed at flowering stage and the least difference was observed in waterlogging stress at all three growth stages. In Omrani cultivar, the highest grain yield reduction was observed in waterlogging stress at all three growth stages, and the least grain yield decrease at flowering stage. Overall, the reductions in dry weight and grain yield were higher in Omrani than Hindi cultivar at three growth stages. Therefore, in stress-prone areas such as Ahvaz where summer plants such as Mung bean are exposed to waterlogging stress at different growth stages, Hindi cultivar is suggested for cultivation. Waterlogging stress did not show a significant effect on grain weight and grain number per pod, but significantly reduced the number of pods per plant. The number of pods per plant was significantly more important in determining yield and had a positive and significant correlation (r = 0.846 **) with grain yield. The reason of difference between two cultivars was the better physiological compatibility of Hindi cultivar under stress conditions, including higher levels of photosynthesis and photosynthetic pigments contents. When plants were treated only with waterlogging stress in one growth stage, the Omrani cultivar could compensate some damages due to its indeterminate growth and phenological flexibility, as the period of pod formation was longer in this cultivar. SPAD value had the highest correlation with grain yield (r=0.707**) compared to other photosynthesis-related traits and could be used as a marker trait for selection of tolerant cultivars because of its ease, speed, cheapness and the possibility to measure this trait in the field. ConclusionThe results indicated that mung bean yield decrease in response to waterlogging stress, depending on growth stages, and experimental duration. The reductions in grain yield was impacted by the growth stage subjected to waterlogging stress. Moreover, the Hindi cultivar seems to be more tolerant to waterlogging in Khuzestan region

Research Paper - Agronomy

Microtuberization Efficiency of Three Potato (Solanum tuberosum L.) Cultivars under Osmotic Stress in vitro

Banafsheh Jamshidi; Marzieh Ghanbari Jahromi; Amir Mousavi

Volume 45, Issue 1 , May 2022, Pages 109-122

https://doi.org/10.22055/ppd.2020.30032.1782

Abstract

IntroductionPotato (Solanum tuberosum L.) is one of the most important crops worldwide. It is vegetatively propagated using pieces or whole potato tubers, but a virus infection causes ... Read More IntroductionPotato (Solanum tuberosum L.) is one of the most important crops worldwide. It is vegetatively propagated using pieces or whole potato tubers, but a virus infection causes crop reduction to almost a half or even one third, which varies from place to place and from season to another. Micropropagation is the practice of rapidly multiplying stock plant material to produce many progeny plants, using modern plant tissue culture methods. Microtuberization in potato needs the right interaction between several factors such as cyto, sucrose and osmotic stress. The purpose of present study was to investigate microtuberization efficiency of three potato cultivars under osmotic stress and in vitro conditions. Materials and MethodsThis experiment was carried out based on completely randomized design (CRD) in three replications. Potato seeds of three cultivars (Agria, Savalan and HPS-II/67) were cultured on Murashige and Skoog (MS) medium. After proliferation, plantlets were transferred to the media containing nine treatments with polyethylene glycol hydrogel (PEG) at four levels (0.003, 0.006, 0.009, 0.012 M), sorbitol at four levels (0.1, 0.2, 0.3, and 0.4 M). For this purpose, potato quality, microtuberization percentage, proline concentration, chlorophyll content, and Catalase (CAT) and superoxide dismutase (SOD) activities were measured. Results and DiscussionThe results showed that plantlets grown under non-stressed conditions (control) possessed higher rates of leaf number, new shoot length and proliferation index compared to the under stressed conditions. Agria cultivar presented better growth characteristics under stressed conditions compared to other experimental cultivars. The highest potato destruction was observed at 0.012 M PEG in Savalan cultivar followed by 0.4 M sorbitol in all cultivars. Microtuberization percentage increased in all three cultivars by stress conditions, in which it increased with progressing in stress levels. The greatest percentage of microtuberization was obtained from Agria cultivar at 0.003 M PEG. Compared to the control, proline concentration, and also CAT and SOD activities increased under stress conditions. Moreover, total chlorophyll content decreased under the stress conditions in comparison to the control. Generally, based on the results, maximum rates of microtuberization and viability were observed in Agria followed by HPS-II/67, and the highest microtuber weight was obtained in HPS-II/67 cultivar. When plants are faced to stress conditions like osmotic stress, free radicals such as reactive oxygen species (ROS) increases. On the other hand, plants use different strategies to scavenge the generated ROS. The reduction of chlorophyll content and increases of antioxidant enzyme activities are due to increase of ROS under osmotic stress conditions. ConclusionAt slight stress conditions, potato plants utilize the strategy to generate more tuber. Therefore, the lower concentrations of PEG can be introduced as a stimulator of microtuber.

Research Paper - Weed Sciences

Cogongrass (Imperata cylindrica L. Beauv) Management in Sugarcane Fields of Subtropical Iran

Farkhondeh Eskandari; Batool Keramat; Yousef Filizadeh; Amir Aynehband

Volume 45, Issue 1 , May 2022, Pages 123-132

https://doi.org/10.22055/ppd.2020.33905.1919

Abstract

IntroductionAmong all weeds, cogongrass (Imperata cylindrica L. Beauv) has been identified as a problematic weed in the sugarcane (Saccharum officinarum L.) fields of Iran for over ... Read More IntroductionAmong all weeds, cogongrass (Imperata cylindrica L. Beauv) has been identified as a problematic weed in the sugarcane (Saccharum officinarum L.) fields of Iran for over 20 years since 1996. Failure to control of cogongrass regrowth during spring develops it rapidly and, in turn, reduces the sugarcane yield in subtropical Iran. In effect, the identification of weak points in cogongrass phenology and frequency is deemed to help decision makers adopt effective controlling strategies in optimum time. Storage of carbohydrate reserves by plants is, in turn, the best mechanism for optimizing the timing of management strategies when reserves are at the lowest levels in storage organs. Glyphosate has been also widely used in Iran to control of weeds in the sugarcane fields. Currently, despite double spraying, the spring control of cogongrass is not satisfactory in the sugarcane fields in subtropical Iran. Materials and MethodsTwo studies were carried out in Salman Farsi Agro-Industrial Company in about 40 km south-west of the center of Khuzestan Province, Ahvaz, Iran, in 2017. In the first experiment, monthly (February–November) total nonstructural carbohydrate allocation patterns of cogongrass were evaluated to determine carbohydrate low points in the seasonal phenological cycle. To that end, glyphosate (00, 0.92, 1.84, 2.76 and 3.68 L a.i ha-1) was applied in April and June, 2017, in a 10×2 factorial design under randomized complete block designs with four replications. Data were analyzed using standard ANOVA procedures with the subsequent use of Least Significant Difference test (Little and Hills 1978( to separate means. Results and DiscussionThe occurrence of carbohydrate low points (CLPs) (88.69±13.15 mg g-1) was considered from May to August, CLP was found to have two important points with the potential control of cogongrass reported. The first and second points were in May (579 GDD) and August (2683GDD), respectively. The suppression of cogongrass (58-100%) was achieved with the three highest rates (1.84, 2.76 and 3.68 L a.i ha-1) in both application times, although substantial regrowth occurred in all plots by 5 months after the first treatment. Double spraying with a 60-day interval did not result in the effective control of such a troublesome weed in the sugarcane fields. Applying glyphosate two times in the spring and mid-summer (the end of CLP, 2683 GDD) was determined effective for the long-term control (>6 months) of cogongrass. The results suggest that glyphosate 1.84 L a.i ha-1 should be performed on cogongrass in the spring for improved control. ConclusionWe concluded that frequent spring spraying may be excluded to control of cogongrass in the sugarcane fields and only spraying should be made on twice the growth seasons. The first use of glyphosate in its minimum optimum dose (1.84 L a.i ha-1) is made on early spring. The second is sprayed in its maximum optimum dose (3.68 L a.i ha-1) at the midsummer, once the single-sprayed cogongrass gets appropriate leaf surface for transferring the herbicide.

Research Paper - Weed Sciences

Application of Chlorophyll Fluorescence Parameter for Rapid Detection The Effect of Photosystem II Inhibiting Herbicides on Resistant and Susceptible Junglerice (Echinochloa colona (L.) Link.) Populations

Elham Elahifard; Ali Ghanbari; Mohammad Hassan Rashed Mohassel; Eskandar Zand; Amin Mirshamsi Kakhki

Volume 45, Issue 1 , May 2022, Pages 133-142

https://doi.org/10.22055/ppd.2020.30996.1820

Abstract

IntroductionChlorophyll fluorescence induction is a rapid and noninvasive technique for measuring photosynthetic electron transport in plants. Recognition of fluorescence curves such ... Read More IntroductionChlorophyll fluorescence induction is a rapid and noninvasive technique for measuring photosynthetic electron transport in plants. Recognition of fluorescence curves such as Kautsky curves is a useful tool for quantifying herbicide effects. In general, since one of the goals of measuring chlorophyll fluorescence is the early detection of the effects of herbicide before its apparent effects on weed. PSII inhibiting herbicides resistance results from a mutation in the chloroplast genome, reducing the rate of electron transport between QA and QB in PS II. Materials and MethodsThe seeds pre-germinated in Petri dishes at 28±2 0C in 16/8 hr (light/dark) photoperiod for 72 hr. The pre-germinated seeds were sown in pots (with 10 cm diameter) containing loam: sand 2:1 mixture (v/v). Pots were transferred to a greenhouse and grown at 30 0C and 20 0C day and night temperatures, respectively, with artificial light to provide a 16-h photoperiod. Pots were irrigated regularly to avoid any moisture stress. Ten days after planting (DAP), they were thinned to two seedlings per pot. Twenty days after weed emergence, seedlings of the pots were subjected to the post emergence application of ametryn. Different ranges of ametryn rates were used for the resistant (0, 10, 30, 100, 300, 1000, 3000, 10000 and 30000 g ai ha-1) and susceptible (0, 1, 10, 30, 100, 300, 1000 g ai ha-1) populations because of the difference in dose-response among populations. Nonionic surfactant 0.25% (v/v) was applied with the herbicide at the time of spraying. The sprayer was calibrated to deliver 220 L ha-1 at pressure of 2 atm. The aboveground biomass was harvested 28 days after treatment (DAT) and weighed. In order to analyze the data and draw the shapes, Gompertz function and SigmaPlot11 and R soft wares were used. PEA Plus software, also, was used to output the chlorophyll fluorescence data recorded during the experiment. Results and DiscussionThe results showed that Fvj was more sensitivity to the application of ametryn in the tested populations. So that four hours after the application of ametryn, Fvj, in some populations, especially susceptible population (24.47 g ai ha-1), decreased significantly. Regarding ED50 values based on fresh weight of populations, the highest amount of ED50 belonged to R4 (10289 g ai ha-1), R1 (6844.6 g ai ha-1), R3 (4770 g ai ha-1) and R2 (3041.0 g ai ha-1), respectively. Estimated ED50 values for Fvj susceptible and resistant populations were, in some cases, far lower than estimated ED50 values for fresh weight of populations at 28 days after treatment with herbicides. In general, the linear relationship between the above parameter and the fresh weight of the populations indicates a strong correlation between these parameters. There were differences among the resistant and susceptible populations in the application of herbicide in terms of the slope of the curve of fvj. So that the curve slope in R1 and R2 biotypes was faster than other populations. In susceptible population response, more correlation was observed between the above parameters. The purpose of this parameter was to show that chlorophyll fluorescence parameters, such as fresh weight assay or weed dry weight, can predict the effect of herbicides. Thus, the researchers are able to evaluate the herbicide's impact on the plants by spending less time and cost. ConclusionSince the main effect of photosystem II inhibiting herbicides is in the J stage (Fvj). Therefore, it is possible to use the Fvj parameter, which most likely shows the changes in electron transfer in the electron transfer chain in photosystem II. The most resistant populations (R1 and R4) based on the measurement of fresh weight, in the analysis based on the Fvj parameter, like other resistant populations, showed the significant difference with the susceptible population. Also, the sensitivity of the susceptible population response to herbicide application based on the Fvj parameter was much higher than that of fresh weight. So that it enables the researcher to quickly distinguish among the susceptible and resistant populations to the mentioned herbicides.

Research Paper - Modeling

Construction of Critical Nitrogen Dilution Curve Based on Stem Dry Matter in Rapeseed

SeyedAtaollah Siadat; Abolfazl Derakhshan

Volume 45, Issue 1 , May 2022, Pages 143-156

https://doi.org/10.22055/ppd.2020.31971.1854

Abstract

IntroductionThere are mathematical equations to distinguish nitrogen (N) status based on the concept of critical nitrogen (Nc), the lowest N concentration required to achieve maximum ... Read More IntroductionThere are mathematical equations to distinguish nitrogen (N) status based on the concept of critical nitrogen (Nc), the lowest N concentration required to achieve maximum growth. The Nc demonstrates the ideal N status in crops. Whenever crop N concentration is greater than the Nc, at that time N is in surplus, but if it is less, then N is inadequate. The contribution of the stem dry matter (SDM) toward total crop dry matter is significantly higher than that of other tissues, therefore, it is the most determinant factor for N dilution of the whole plant. The objectives of this research were to construct a Nc dilution curve based on SDM and to evaluate the plausibility of this curve for assessment of the N nutrition status of spring rapeseed. Materials and MethodsA field experiment was conducted with seven levels of N fertilizer application (0, 50, 100, 150, 200, 250 and 300 kg N ha-1) in which the amount of the SDM and stem N concentration (SNC) of two spring rapeseed cultivars (Hyola 401 and Dalgan) were measured during the growing season. The approach proposed by Justes et al. (1994) was used to construct the SDM basis Nc dilution curve. The N nutrition index (NNI) on each sampling date was calculated by dividing actual SNC by stem Nc concentration. The accumulated N deficit (Nand) in rapeseed stems for each sampling date was determined by subtracting the N accumulation in stems under the Nc condition from actual N accumulation in stems under different N fertilizer application rates. Results and DiscussionOverall, both SDM and SNC showed an increasing and decreasing trend with increasing crop age, respectively, regardless of fertilizer treatment. The Nc dilution curve of both cultivars during the growing season was described by the equation Nc = 5.71SDM-0.29. Both NNI and Nand criteria were able to diagnosis the N deficiency or excess in the spring rapeseed cultivars studied. At all of the crop growth stages, there was a significant positive correlation between the difference value of the N application rate (∆N) and the difference value of NNI (∆NNI). For both cultivars studied, however, the positive correlation between ΔN and differences in Nand among N fertilizer treatments (ΔNand) was not significant at early growth stages. In any case, for accurate management of N fertilizer during the growing season, it is sufficient to determine only one of the two NNI or Nand criteria per day from planting to silique formation, and then the N fertilizer required for maximum growth is calculated based on the equations presented to describe the relationships between ΔN and ΔNNI or between ΔN and ΔNand. The most important application of the Nc dilution curve is to determine the N requirement of crops during the growing season under variable field conditions. These curves can be used as ideal and economically efficient tools for accurate management of fertilizer in the field, which is very important from the aspect of environmental protection and human health. Both NNI and Nand were well able to distinguish the N status of spring rapeseed. ConclusionThe present Nc dilution curve based on SDM provides an insight into N nutrition status in spring rapeseed crop and can serve as a novel tool to improve N fertilization management in spring rapeseed.

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Volume & Issue: Volume 45, Issue 1, May 2022, Pages 1-156

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