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Molecular characterization of recomMolecular Characterization of Recombinant Inbred Line Population of Rice With Retrotransposon (Ipbs) MarkerRetrotransposon iPBS

Document Type : Research Paper

Authors
S. Bakhtiari 1
D. Nabati Ahmadi 2
K. Sorkheh 3
M. Hosseni Chaleshtari 4
1 M.Sc. Student of Plant Breeding, Department of Agronomy & Plant Breeding, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Associate Professor, Department of Agronomy & Plant Breeding, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 Assistant Professor, Department of Agronomy & Plant Breeding, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4 Assistant Professor of Rice Research Center, Agricultural Research Education, Rasht, Iran
10.22055/ppd.2017.16723.1304
Abstract
Abstract
Background and Objectives
In genetic studies in addition to utilization of molecular markers based on genomic DNA, one can also use retrotranspostion or jumping genes as a marker to identify genetic characterization and diversity in the plant species. Since the retrotransposesons have transferable motive they tend to move along the genome. The movement is a key figure to induce alteration at the genomic structure. Thus, this reinforces the genome to boost the expression of some genes and/or silence the other genes.  Furthermore, retrotranspostions are able to affect the activity of genes transcription at the organelles and biotic and abiotic stresses.
Materials and Methods
In the present study, retrotranspostion iPBS marker was used to assess genetic characterization of 130 inbred lines of rice which were derived from hybridization of Neda and Hashmi rice cultivars. In this study, 4 primers were used which were able to produce 2990 bands and among them1449 were polymorphic.
Results
According to amplification of polymorphic primers analysis, R2020 primer showed totally 443 polymorphic bands and had 56.79% bands with the highest polymorphism among all the primers. None of primers showed any single polymorphic band.
Discussion
Cluster analysis classified inbred lines of rice into two groups. Parental lines along with 146, 148, 149, 151 and 154 lines were in one group and 67, 68 and 82 lines were fit into another group with close distance to the parental lines. In terms of the presence of retrotransposition genes these lines possess the most genetic similarity to the parental lines. The information which was revealed by the findings of the presented study indicated that iPBA markers can be assigned to trace the genetic mapping in order to assess the molecular genetics of the characteristics of the line within a  plant population in the breeding program.

Cluster analysis classified inbred lines of rice into two groups. Parental lines plus 146, 148, 149, 151 and 154 lines were in one group and 67, 68 and 82 lines were fit into another group which they had closed distance to the parental lines. In term of the presence of retrotransposition genes these lines possess the most genetic similarity to the parental lines
In genetic studies in addition to utilize molecular markers based on genomic DNA, one can also use retrotranspostion marker or jumping genes to identify genetic characterization and diversity in the plant species. In the present study retrotranspostion iPBS marker was used to assess genetic characterization of 130 inbred lines of rice which derived from hybridization of Neda and Hashmi rice cultivars. In this study 4 primers have used which were able to produce 2990 bands and among them1449 were polymorphisms. According to amplification of polymorphic primers analysis, R2020 primer showed totally 443 polymorphism bands and with having of 56.79% bands had the highest polymorphism bands among all the primers. None of primers have shown single polymorphic band. Cluster analysis classified inbred lines of rice into two groups. Parental lines plus 146, 148, 149, 151 and 154 lines were in one group and 67, 68 and 82 lines were fit into another group which they had closed distance to the parental lines. In term of the presence of retrotransposition genes these lines possess the most genetic similarity to the parental lines.
Keywords
Hashemi rice
iPBS
Neda rice
Retrotransposons
Subjects
References
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Plant Productions
Volume 40, Issue 4
Winter 2018
Pages 1-10