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Increasing the Expression of Genes Chavicol O-Methyl Teransferase and Cinnamate 4-Hydroxilase under Methyl Jasmonate Treatment in Medicinal Plant Basil (Ocimum basilicum L.)

Document Type : Research Paper

Authors
Leyla Hassani 1
Babak Abdollahi Mandoulakani 2
Reza Darvishzadeh 3
Abbas Hassani 4
1 MSc student
2 Associate professor Department of Plant Breeding and Biotechnology, College of Agriculture, Urmia University,
3 professor Department of Plant Breeding and Biotechnology, College of Agriculture, Urmia University
4 Associate Professor, Department of Horticulture, Urmia University
10.22055/ppd.2016.11961
Abstract
Background and Objectives
Basil is a rich source of phenylpropanoids and enzymes Chavicol o-methyl transferase and cinnamate 4-hydroxylase are involved in the biosynthesis of these compounds.
Material and Methods
A completely randomized design with three replications was conducted in greenhouse to assess the effect of methyl jasmonate on the expression of the genes encoding CVOMT and C4H enzymes. Methyl jasmonate with concentrations of 0, 0.1 and 0.5 mM was sprayed on healthy plant leaves in flowering stage. Plant leaves were sampled at 0, 24, 48 and 72 hours after spraying. Relative expression of the genes was determined by real time PCR. Methyl jasmonate concentrations and sampling times were considered as main and sub-plots, respectively, and analysis of variance was performed in a split plot in time design.
Results
Based on the results of variance analysis and mean comparison tests, the highest expression of the CVOMT gene was achieved in a concentration of 0.5 mM methyl jasmonate at 48 hours after spraying (p≤0.01). The expression of the C4H gene was significantly (p≤0.05) affected by the methyl jasmonate concentrations and sampling times. The maximum expression of this gene was obtained in 0.1 mM concentration of methyl jasmonate. Also, the expression of C4H gene was reached its peak 48 hours after spraying.
Discussions
Methyl jasmonate with concentrations of 0.1 and 0.5 mM methyl jasmonate lead to significant increase in the expression of the genes encoding CVOMT and C4H enzymes which could probably increase the production of phenylpropanoids such as chavicol and methyl chavicol.
 
 
 
 
Keywords
Methyl jasmonate
Basil
Real time PCR
Chavicol o-methyl teransferase
Subjects
  1. Begum, F., Amin, M.N., and Azad, M. 2002. In vitro rapid clonal propagation of Ocimum basilicum Plant Tissue Culture, 12: 27-35.
  2. Bi, H.H., Zeng, R.S., and Su, L.M. 2007. An M and Luo SM. Rice allelopathy induced by methyl jasmonate and methyl salicylate. Journal of Chemical Ecology, 33(5): 1089-103.
  3. Bourgaud, F., Gravot, A., and Miles, S. 2001. Production of plant secondary metabolites: A historical perspective. Plant Science, 161(5): 839-851.
  4. Da Costa, A.S., de Fatima, M., Blank, A., Filho, J.L.S., de Santana, A.D.N.D.,
    Santos, D.A.P.B., and Arie, F.B. 2015. Chemical Diversity in Basil (Ocimum ) Germplasm. The Scientific World Journal, 1-9.
  5. Ellard Ivey, M. and Douglas, C.J. 1996. Role of jasmonates in the elicitor and wound inducible expression of defense genes in parsley and transgenic tobacco. Plant Physiology, 112: 183-192.
  6. Esmailzadeh behabadi, S. and Sharifi, M. Increasing the production of plant secondary metabolites using biotic elicitors. Journal of Cell and Tissue, 4(2): 119-128. [In Farsi]
  7. Frank, M.R., Deyneka, J.M., and Schuler, M.A. 1996. Cloning of wound induced cytochrome P450 monooxygenases expressed in pea. Plant Physiology, 110: 1035-1046.
  8. Gang, D.R., Wang, J. Nam, K.H., Simon, J.E., Lewinsohn, E., and Putivisky, E. 2001. An investigation of the storage and biosynthasis of phenylpropenes in sweet basil. Plant Physiology, 125: 539-555.
  9. Grayer, R.J., Vieira, R.F., Price, A.M., Kite, G.C., Simon, J.E., and Paton, A.J. 2004. Characterization of cultivars within species of ocimum by exudate flavonoid profiles. Biochemical Systematics and Ecology, 32(10): 901-913.
  10. Hahlbrock, K., Scheel, D., Logemann, E., Nurnberger, T., Parniske, M., Reinold, S., Sacks, W.R., and Schmelzer, E. 1995. Oligopeptide elicitor-mediated defense gene activation in cultured parsley cells. Proceedings of the National Academy of Sciences of the United States of America, 92: 4150-4157.
  11. Ishihara, , Ohtsu, Y., and Iwamura, H. 1999. Induction of biosynthetic enzymes for avenanthramides in elicitor-treated oat leaves. Planta, 208: 512-518.
  12. Kuchaki, A., Nasiri Mahallati, M., Hassanzadeye avval, F., and Mansuri, H. 2013. Vegetables biodiversity assessment in Iran agroecologys. Iranian journal of Applied Ecology, 2(4): 1-11. [In Farsi]
  13. Lewinsohn, E., ZivRaz, I., Dudai, N., Tadmor, Y., Lastochkin, E., Larkov, O., Chaimovitsh, D., Ravid, U., Putievsky, E., Pichersky, E., and Shoham, Y. 2000. Biosynthesis of estragole and methyleugenol in sweet basil (Ocimum basilicum ) developmental and chemotypic association of allylphenol O-methyltransferase activities. Plant Science, 160: 27-35.
  14. Liu, S., Hu, Y., Wang, X., Han, L., Song, S., Cheng, H., and Lin, Z. 2009. Isolation and characterization of a gene encoding cinnamate 4-hydroxylase from Parthenocissus henryana. Molecular Biology Reports, 36:1605-1610.
  15. Marotti, M., Dellacecca, V., Piccaglia, R., and Glovanelli, E. 1997. Agronomic and chemical evaluation of three varieties of Foeniculum vulgare Acta Horticulturae, 331: 63-69.
  16. Memelink, J. 2009. Regulation of gene expression by jasmonate hormones. Phytochemistry, 70 (13-14): 1560-1570.
  17. Naderi, S., Fakheri, B., and Esmailzadeh bahabadi, S. 2014. Increasing of chavicol o-methyl transfrase gene expression and catalase and ascorbate peroxidase enzymes activity of Ocimum basilicum by chitosan. Journal of Crop Biotechnology, 3(6):1-9. [In Farsi]
  18. Ramos, R., Tovar, F., Junqueira1, R., Lino,, and Martins, G. 2001. Sugarcane expressed sequences tags (ESTs) encoding enzymes involved in lignin biosynthesis pathways. Genetics and Molecular Biology, 24: 235-241.
  19. Raouf Fard, F., Omidbaigi, R., Sharifi, M., Sefidkon, F., and Behmanesh, M. 2011. Effect of methyl jasmonate on essential oil content and composition of agastache foeniculum. Journal of Medicinal Plants Research, 6(45): 5701-5705.
  20. Rauf Fard, F. Sharifi, M., Omidbaigi, R., Sefidkon, F., Behmanesh, M., and Ahmadi, N. 2014. Effect of methyl jasmonate on metabolic enzymes and phenolics, in agastache foeniculum [pursh] kuntze. Iranian Journal of Medicinal and Aromatic Plants, 3: 361-369.
  21. Salzman, R.A., Brady, J.A., Finlayson, S.A., Buchanan, C.D., Sun, F., Klein, P.E., Klein, R. R., Pratt, L. H., Cordonnier-Pratt, M.M., and Mullet, J.E. 2005. Transcriptional profiling of sorghum induced by methyl jasmonate, salicylic acid, and aminocyclopropane carboxylic acid reveals cooperative regulation and novel gene responses. Plant Physiology, 138: 352-368.
  22. Tahsili, J., Sharifi, M., Behmanesh, M., and Ziaei, M. 2011. Gene expression of eugenol o-methyl transferase and components of essential oils in (Ocimum basilicum) at different stages of growth. Iranian Journal of Biology, 18: 23-25. [In Farsi]
  23. Taile, H.A.A., Salama, Z.A., and Radwan, 2010. Potential activity of basil plants as a source of antioxidants and anticancer agents as affected by organic and bio-organic fertilization. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38: 119-127.
  24. Turgut-kara, N. and Ari, S. 2011. Analysis of elicitor inducible cytochrome P450 induction in Astragalus chrysochlorus Plant Omics, 4(5): 264-269.
  25. Werker, E., Putievsky, E., Ravid, U. Dudai, N., and Katzir, I. 1993. Glandularh and essential oil in developing leaves of Ocimum basilicum (Lamiaceae). Annals of Botany, 71: 43-50.
  26. Yu, Z.X., Wang, L.J., Zhao, B., Shan, C.M., Zhang, Y.H., Chen, D.F., and Chen, X.Y. 2015. Progressive regulation of sesquiterpene biosynthesis in arabidopsis and patchouli (Pogostemon cablin) by the miR156-targeted SPL transcription factors. Molecular Plant, 8: 98-110.
  27. Zare Mehrjerdi, M., Bihamta, M.R., Omidi, M., Naghavi, M.R., and soltanloo, H. 2014. Study on Artemisia annua and Arabidopsis thaliana trichome genes in response to methyl jasmonate and salicylic acid elicitors. Modern Genetics Journal, 3: 329-332. [In Farsi]
  28. Zarei, H. Fakheri, B.A., Bahabadi, S.E., and Solouki, M. 2015. Increasing of chavicol o-methyl transferase gene expression (CVOMT) and methyl chavicol value of basil (Ocimum basilicum) by salicylic acid. Journal of Biodiversity and Environmental Science, 6(3): 46-53.
Plant Productions
Volume 39, Issue 3 - Serial Number 3
Autumn 2016
Pages 101-112