Plant Productions

Effect of foliar application of amino acids on some physiological processes of oil rapeseed (Brassica Napus L.) under late-season drought stress conditions

Esmaeil Fayaz; ALI SOROOSHZADEH; Ali Heidarzadeh

Articles in Press, Accepted Manuscript, Available Online from 09 March 2024

https://doi.org/10.22055/ppd.2024.45873.2138

Abstract

Rapeseed (Brassica napus L.) is one of the most valuable oilseed plants cultivated in the world. Cultivation of this plant as the third source of oil production is expanding due to ... Read More Rapeseed (Brassica napus L.) is one of the most valuable oilseed plants cultivated in the world. Cultivation of this plant as the third source of oil production is expanding due to its nutritional and economic advantages. However, due to Iran's arid and semi-arid climate, the cultivation of this plant is affected by water deficit stress at the end of the season. Various methods are used to reduce the adverse effects of drought stress and increase plant tolerance. One of these methods is using biological stimulants such as amino acids. These compounds are able to stimulate plant growth and development under optimal conditions and stress. Amino acids have a direct and indirect role in physiological metabolism, exchange, and structural activities, such as increasing the concentration of photosynthetic pigments and the rate of photosynthesis and protein synthesis under stress conditions. Considering the importance of oil production in the country and the important role of rapeseed in oil production, as well as the problems of water scarcity in the reproductive growth stages of rapeseed, this research was conducted to investigate the effect of foliar spraying of amino acids on the physiological parameters of rapeseed in the condition of interruption of irrigation at the end of the season. Materials and methods This experiment was conducted to investigate the effect of foliar spraying of amino acids on the physiological characteristics and chlorophyll fluorescence parameters and photosynthetic pigments of oilseed rape under withholding irrigation at the end of the season (reproductive growth stage) as factorial design based on randomized complete blocks with three replications in the research farm of the Faculty of Agriculture of Tarbiat Modares University, during the 1401-02 growing season. The investigated factors were three levels of irrigation (full irrigation during the season, withholding irrigation from 50% flowering stage, withholding irrigation from pod forming) and five levels of foliar spraying (no foliar spraying, zero foliar spraying (distilled water), one, two and three grams of amino acids per liter) in stem elongation, flower-bud emergence, and flowering stages. The experimental plots included four planting rows and three meters in length. In the field, one week after the last foliar spraying, photosynthetic and transpiration rates and various fluorescence parameters were measured using a portable gas exchange and Mini-PAM device, respectively. Moreover, pigments (including chlorophyll a, b and carotenoid) in the fresh plant samples were determined in the laboratory. After ensuring the normality of the data, statistical analysis was performed using SAS software (version 4.9.) to compare the average data. The LSD test was used at the five percent level. Results All physiological and biochemical traits were affected by water deficit stress (withholding irrigation), which caused a significant decrease in characteristics, such as maximum photosystem efficiency, photosynthesis rate, stomatal conductance, transpiration rate and photosynthetic pigments. However, foliar application of amino acids reduced the adverse effect of drought stress. The results showed photosynthesis rate, stomatal conductance, transpiration rate, intercellular CO2 concentration and Intercellular CO2/ambient CO2, photosynthetic pigments (chlorophyll a, chlorophyll b and carotenoids) and chlorophyll fluorescence parameters were significantly affected by the interaction of drought stress and foliar spraying of amino acid. The highest photosynthesis rate (12.27 μmol CO2 m-2 s-1), stomatal conductance (0.153 mol H2O m-2 s-1), transpiration rate (1.33 mmol H2O m-2 s-1), chlorophyll b (832 0.0 mg.g-1FW) and carotenoid (0.812 m mg.g-1FW) were obtained by the foliar treatment of two grams of amino acids per liter, while the chlorophyll a (2.03 mg.g-1FW) and Fv/Fm (0.832) were obtained by spraying with one gram of amino acids per liter. Conclusion According to the results, the most positive effect of the foliar application of amino acids on the studied traits was two grams per liter of water. On the other hand, improving these traits directly affects seed yield. Therefore, considering the direct effect of these traits in improving seed yield, foliar spraying of two grams of amino acids per liter is recommended to improve the physiological and biochemical characteristics of rapeseed in all three irrigation regimes (full irrigation, interruption of irrigation from the flowering and budding stage).

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Effect of Methanol Spraying on some Morphophysiological Characteristics, Yield and Yield Components of Sunflower (Helianthus annuus L.) under Drought Stress Conditions

Hamed Javadi; Seyyed Gholam Reza Moosavi; Mohammad Javad Seghatoleslami; Ali Reza Ebrahimi; Mohammad Kozegar

Volume 45, Issue 1 , May 2022, , Pages 29-40

https://doi.org/10.22055/ppd.2020.33127.1888

Abstract

IntroductionSunflower (Helianthus annuus L.) is one of the five important oil plants of Iran. Water deficit stress is one of the most important limiting factors in sunflower cultivation. ... Read More IntroductionSunflower (Helianthus annuus L.) is one of the five important oil plants of Iran. Water deficit stress is one of the most important limiting factors in sunflower cultivation. Methanol is used to deal with environmental stresses, especially dehydration. Preventing or reducing light respiration, delaying leaf senescence, increasing photosynthetic activation period and leaf area duration index and increasing CO2 fixation are among the roles of methanol application in plants. Therefore, this study was conducted to investigate the effect of methanol spraying on some morphophysiological characteristics, yield and yield components of sunflower under drought stress conditions. Materials and MethodsThis field experiment was conducted as a split plot based on randomized complete block design with three replications in Birjand, Iran, in 2017. The main plots included two irrigation levels (irrigation after 80 and 160 mm evaporation form class A pan). On the other hand, the sub- plots included five levels of methanol spraying; control (spraying with water), 7, 14 and 21% v/v. Statistical analysis was performed using MSTATC software. Duncan's multiple range test at the 5% level of probability was used to compare the means. Results and DiscussionResults showed that deficit irrigation reduced stem diameter, number of grains per head and kernel/husk weight ratio. Methanol spraying also increased the number of grains per head. Also, methanol spraying improved most of the studied traits under two irrigation treatments. So that under drought stress conditions, foliar application with 28% volumetric methanol increased plant height (9.9%), stem diameter (5%) and leaf number per plant (14.7%). Compared with control, foliar application with 14% and 21% volumetric methanol increased SPAD by 8% and 34.7%, respectively and also, increased 1000-grain weight (12%), grain yield (27.9%) and biological yield (23.4%). According to the results, under optimum irrigation conditions, maximum sunflower grain yield was obtained with 28% volumetric methanol spraying, but under drought stress conditions, spraying with 21% volume methanol was the best one. ConclusionThe results showed that foliar application of methanol under optimum and also deficit irrigation conditions did not affect the percentage and yield of sunflower oil seed, but increased grain yield. Maximum grain yield under optimum irrigation was obtained with 28% volumetric spraying, which was 27% more than control. Also, the results showed that under arid and semi-arid conditions of Birjand, deficit irrigation with using methanol (21% volumetric) produces acceptable grain yield.

The Growth and Physiological Characteristics of Three Commercial Rootstocks of Pistachio (Pistacia vera L.) Under Controlled Temperature Conditions

H. Hokmabadi; M. Rezaei; H. Hokmabadi

Volume 41, Issue 2 , September 2018, , Pages 79-90

https://doi.org/10.22055/ppd.2018.20314.1423

Abstract

Abstract Background and Objectives In recent decades, global warming causes climate changes which triggers physiological and nutrition disorders in plants. Iran is a main pistachio ... Read More Abstract Background and Objectives In recent decades, global warming causes climate changes which triggers physiological and nutrition disorders in plants. Iran is a main pistachio producer in the world. Pistachio cultivar is mainly grafted into seedling rootstocks are which obtained from especial cultivars and wild type pistachio. The changes in the vegetative growth and yield in pistachio orchards have been obviously observed in the years with so warm or so cold weather conditions. The purpose of this research was evaluating the effects of temperature on the growth of three pistachio rootstocks. Materials and Methods In order to study the effect of temperature on the growth and physiological characteristics of three commercial pistachio rootstocks, a factorial experiment on the basis of a completely randomized design (CRD) was carried out with three replications. The first factor was three rootstocks including: Pistacia vera cv. Badamie-zarand, P. vera cv. Ghazvini and P. vera var. Sarakhsi and the second factor was four temperature conditions including: 10, 15, 20 and 25 degrees Celsius. Pistachio seedlings after planting up to 15-leaf stage were kept in the greenhouse condition and then the pots were transferred to growth chambers which adjusted to defined temperature treatments. The plants were kept in temperature treatments for fifteen days. At the end of experiment, growth parameters including fresh and dry leaf weight, fresh and dry weight in shoot and root and leaf area and physiological parameters including stomatal conductance, relative water content of leaves and leaf chlorophyll index were measured. The statistical analysis was done using SAS 9.1 software and mean comparison was done by using the lowest significant difference test (LSD). Results The results showed that with increasing environmental temperature, an increase in the number of leaves, stem height, dry weight of leaf, stem and root, leaf area was observed in pistachio rootstocks. There were significant differences among rootstocks in most of the growth parameters. Badamie-Zarand was more vigorous than the other rootstocks in low temperature. The highest SPAD index was observed in high temperature environment (20, 25°C) beside lowest SPAD index level was observed at Low temperature environmental (10, 15°C). Minimum and maximum stomatal conductance was respectively obtained at 10 and 25 °C in Sarakhsi rootstocks which reveals the sensitivity of this rootstock to temperature fluctuations. In contrast, stomatal conductance variations in the Ghazvini rootstocks are lower than the other rootstocks when exposed to different environmental temperature. This may show the tolerance of this variety to changes of environmental temperature conditions. The leaf RWC in three pistachio rootstocks at different ambient temperatures shows a significant difference. At 15 and 20°C, Ghazvini rootstock has the highest RWC and Badamie- Zarand rootstock has the lowest levels of RWC. The highest relative water content was obtained at 10°C in Sarakhsi rootstock and at 25°C in Badamie-Zarand rootstock. Positive correlation between root dry weight and stomatal conductance reflects the rootstock compatibility with ecological conditions. Discussion Badami Zarand rootstock has more vegetative growth in low temperature environment and more adaptation to a wide range of temperature in comporison to two others rootstock and also can be recommended to nursery as a rootstock for planting.

Comparison of Direct Seeding and Transplanting Systems in Maize (Zea Mays L.) Under The Usual and Early Planting Dates

Zh. Zaraei; H. Heidari; I. Nosratti; M. Khoramivafa

Volume 41, Issue 1 , June 2018, , Pages 97-108

https://doi.org/10.22055/ppd.2018.13553

Abstract

Abstract Background and Objectives The use of transplanting is a safe method for adequate crop establishment. Its benefits include increasing yield, reducing the growing season, improving ... Read More Abstract Background and Objectives The use of transplanting is a safe method for adequate crop establishment. Its benefits include increasing yield, reducing the growing season, improving weed control, increasing water use efficiency, improving seedling establishment and increasing crop growth rate. Transplanting increases crop yield compared with direct seeding, this compensates for more cost of transplanting. Transplanting also increases maize yield compared with direct seeding through increasing radiation use efficiency and harvest index. The experiment was aimed to assess direct seeding and transplanting system under the usual time and early planting date for achieving maximum maize seed yield and dry matter. Materials and Methods The experiment was conducted at Research Greenhouse and Field, Campus of Agriculture and Natural Resources, Razi University during two years (2015 and 2016). In the first year, factors included three planting methods (seeding, 4-week transplanting and 6-week transplanting) and two sowing dates (early sowing, common date sowing). In the second year, treatments included 6- week transplanting at early sowing (superior treatment in the first year) and seeding (control). Studied traits included plant height, leaf number, fresh weight, dry weight, stomatal conductance, SPAD, chlorophyll, leaf area, leaf relative water content and yield traits such as ear weight, ear length and diameter, seed number per ear row, row number per ear, 100-seed weight and seed yield. Data were analyzed by SAS software. Means were compared using LSD test. Results The first year results showed that early sowing increased plant height, SPAD index and specific leaf weight compared to usual sowing date. 6-week transplanting at early sowing had the highest dry matter production and leaf to stem ratio. The second year results showed that transplanting increased the leaf number, chlorophyll content, fresh and dry weight, leaf area, ear length, ear diameter, seed number per ear row, row number per ear, ear weight, seed yield, 100-seed weight and tassel weight compared to direct seeding. Discussion Yield increase in transplanting was probably due to high leaf area and plant height that plants greatly used environmental conditions such as light and water. So seed number per ear row, row number per ear and ear length were high. In conclusion, 6-week transplanting at early sowing (29 March) is suggested to achieve the highest seed yield in Kermanshah. Maize transplant ages except the ages studied in the experiment are recommended for the next experiments.

Heat tolerance evaluating of wheat cultivars using physiological characteristics and stress tolerance indices in Ahvaz climatic conditions

M. Omidi; M.R. Siahpoosh; R. Mamghani; M. Modarresi

Volume 38, Issue 1 , May 2015, , Pages 103-113

https://doi.org/10.22055/ppd.2015.11135

Abstract

In order to evaluate the heat tolerance of 11 wheat cultivars, an experiment was conducted during 2010-2011 under two conditions, normal (normal sowing) and heat stress (late sowing), ... Read More

Short-term responses of stomatal conductance for screening wheat cultivars (Triticum aestivum L.( differing in salt tolerance for osmotic stress tolerance

A. Rahnama; K. Poustini; R. Tavakkol Afshari

Volume 36, Issue 3 , December 2013, , Pages 93-105

Abstract

Salinity affects plant growth by the osmotic stress of salt around the roots as well as by toxicity caused by excessive accumulation of salt in leaves. An investigation was set up to ... Read More

Study of Photosynthesis and Respiration Changes in Different Sugar Beet (Beta vulgaris L.) Genotypes Under Salinity Stress

N A; P H; M M

Volume 35, Issue 1 , June 2012, , Pages 55-69

Abstract

This experiment was conducted to study the effect of salinity stress on growth characteristics, respiration, photosynthesis and gas exchange in three sugar beet genotypes (13030, 22393 ... Read More

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Keywords = stomatal conductance

Effect of foliar application of amino acids on some physiological processes of oil rapeseed (Brassica Napus L.) under late-season drought stress conditions

Abstract

Effect of Methanol Spraying on some Morphophysiological Characteristics, Yield and Yield Components of Sunflower (Helianthus annuus L.) under Drought Stress Conditions

Abstract

The Growth and Physiological Characteristics of Three Commercial Rootstocks of Pistachio (Pistacia vera L.) Under Controlled Temperature Conditions

Abstract

Comparison of Direct Seeding and Transplanting Systems in Maize (Zea Mays L.) Under The Usual and Early Planting Dates

Abstract

Heat tolerance evaluating of wheat cultivars using physiological characteristics and stress tolerance indices in Ahvaz climatic conditions

Abstract

Short-term responses of stomatal conductance for screening wheat cultivars (Triticum aestivum L.( differing in salt tolerance for osmotic stress tolerance

Abstract

Study of Photosynthesis and Respiration Changes in Different Sugar Beet (Beta vulgaris L.) Genotypes Under Salinity Stress

Abstract