تولیدات گیاهی

تولیدات گیاهی

اثرات افزودن سیلیسیم به خاک شالیزار بر صفات مورفولوژیک و تجمع سیلیس در ساقۀ برنج

نوع مقاله : علمی پژوهشی - کشاورزی پایدار

نویسندگان
1 دانش‌آموختۀ کارشناسی ارشد، گروه علوم و مهندسی خاک، دانشکدۀ علوم کشاورزی، دانشگاه گیلان، رشت، ایران
2 استادیار، گروه علوم و مهندسی خاک، دانشکدۀ علوم کشاورزی، دانشگاه گیلان، رشت، ایران
چکیده
سیلیسیم محلول خاک  به صورت اسید مونوسیلیسیک توسط ریشۀ برنج جذب می‌شود. یک شالیزار با عملکرد 5 تن شلتوک در هکتار، حدود 300 کیلوگرم سیلیسیم از خاک جذب می‌کند. در مراحل پنجه‌زنی و ساقه‌دهی، حد بحرانی سیلیسیم در ساقۀ برنج، 5  درصد و حد مطلوب آن حدود 10 درصد وزن خشک است. کمبود سیلیسیم در ساقه باعث خوابیدگی بوته‌ها، افزایش هزینه برداشت و افزایش حساسیت به آفات و بیماری‌ها می‌شود. این پژوهش با هدف بررسی اثر افزودن سیلیس به خاک شالیزار بر ارتفاع، تعداد گره، قطر و میزان سیلیس در ساقه، در دانشکدۀ علوم کشاورزی دانشگاه گیلان در قالب طرح کاملاً تصادفی با 13 تیمار شامل سه سطح از سیلیس از چهار منبع مختلف شامل سیلیس استخراج شده از پوستۀ شلتوک (S1، S2، S3)، خاکستر پوستۀ شلتوک (A1، A2، A3)، پودر پوستۀ شلتوک (P1، P2، P3) و کود تجاری سیلیسیم‌دار (C1، C2، C3) در 39 گلدان حاوی خاک شالیزار اجرا شد. در ابتدای رشد (تیر ۹۸)، ارتفاع ساقه در تمام ۱۳ تیمار حدود ۱۵ سانتی‌متر و بدون تفاوت معنی‌دار بود. اما در آخرین اندازه‌گیری (شهریور ۹۸)، ارتفاع ساقه‌ها تفاوت معنی‌دار یافت و بیشترین ارتفاع (۸۳ سانتی‌متر) مربوط به تیمار S3 بود. همچنین، میانگین تعداد گره در ساقه در تیمار S3 از 3/66  به 7/33 و در تیمار C3، از 3/33 به 6/66 عدد افزایش یافت که تیمار S3 از نظر آماری با سایر تیمارها تفاوت معنی‌دار داشت. در پایان رشد رویشی، قطر ساقه در تیمارهای S3 و C3 به ترتیب 4/98 و 4/97 میلی‌متر بود که به‌طور معنی‌داری بیشتر از سایر تیمارها بود. همچنین این دو تیمار بیشترین مقدار سیلیسیم ساقه را داشتند. در تیرماه، مقدار سیلیسیم در ساقۀ تیمار شده با سیلیس استخراج شده از پوستۀ شلتوک (S3)، 7/06، تیمارشده با کود سیلیسیم دار تجاری (C3) 6/04 و در تیمار شاهد (B) 1/94 درصد به دست آمد. در مرداد ماه نیز مقدار سیلیسیم در 13 تیمار تفاوت معنی‎دار داشت. بیشترین مقدار سیلیس در ساقه در تیمار (S3) معادل 10/79، در تیمار C3،  8/03 و در شاهد (B)، 3/69 درصد بود. در شهریور ماه مقدار سیلیسیم در ساقه روند کاهشی داشت و بیشترین مقدار سیلیسیم ساقه مربوط  به تیمار  S3 و C3 به ترتیب  2/15 و 2/06 درصد بود که فقط با تیمارهای C1  وB  تفاوت معنیدار داشتند. در واقع مقدار سیلیس در ساقه در پایان رشد رویشی در تیمارهایی که سیلیس دریافت کرده بودند، اگرچه بسیار بیشتر از تیمار شاهد بود ولی با هم تفاوت معنیدار نداشتند. با افزایش سن و زیست‌توده گیاه، مقدار سیلیسیم ساقه کاهش یافت که به احتمال زیاد ناشی از انتقال سیلیسیم به پوسته شلتوک در آغاز رشد زایشی است. استفاده از خاکستر پوستۀ شلتوک نسبت به پودر آن، در بیشتر صفات عملکرد بهتری نشان داد. به طور کلی افزودن 0/24 گرم سیلیسیم استخراج شده از پوستۀ بیرونی شلتوک به 3 کیلوگرم خاک (معادل 240 کیلوگرم سیلیس در هکتار) بهترین تیمار بود و بیشترین اثر مثبت را بر رشد برنج داشت. 
کلیدواژه‌ها
موضوعات

عنوان مقاله English

Effects of silica addition to paddy soil on morphological traits and silica accumulation in rice stems

نویسندگان English

Sahar Hosseynpour 1
Reza Ebrahimi Gaskarei 2
1 M.Sc. Graduate, Department of Soil Science, Faculty of Agriculture, University of Guilan, Rasht, Iran
2 Assistant Professor, Department of Soil Science, Faculty of Agriculture University of Guilan, Rasht, Iran
چکیده English

Introduction
Silicon, primarily absorbed by rice roots in the form of H4SiO4, plays a pivotal role in rice cultivation.  It is estimated that approximately 300 kg of silicon is absorbed from the soil per hectare when producing 5 tons of paddy. The critical silicon content in rice during the tillering and ripening stages is 5% of dry weight in the stem and flag leaf. Sufficient silicon levels in the stem help reduce lodging, thus potentially lowering harvesting costs. 
Materials and Methods
 This research was carried out at the Faculty of Agriculture, University of Guilan, using a completely randomized design with 13 treatments and three replications.  The objective was to evaluate the effects of varying silica levels, derived from four different sources, on rice plant height, stem diameter, number of nodes, and silica concentration in the stem. Silica sources included paddy husk, husk ash, husk powder and a siliceous liquid fertilizer. The experiment was performed using 39 pots containing paddy soil. The efficacy of these silica sources was assessed based on changes in plant height, stem diameter, number of nodes, and silica accumulation in both stems and leaves.
 Results and Discussion
 In the initial measurement, no significant differences in stem height were observed among the 13 treatments, indicating that silicon likely requires a longer period to influence stem elongation in rice. However, with extended exposure to silica amendments, marked effects on morphological traits became evident. For example, in the S3 treatment (silica extracted from paddy husk), stem height increased from 15 cm to 8 cm over the growth period.  The average number of nodes in the S3 treatment increased from 3.66 to 7.33 and in the C3 treatment from 3.33 to 6.66, demonstrating a statistically significant difference between the S3 treatment and the others. At the conclusion of vegetative growth, the stem diameters in the control, S3, and C3 treatments were 2.63, 4.98 and 4.97 mm, respectively, with the S3 and C3 treatments significantly outperforming the control.
Silicon uptake and translocation to the stem were highest in the S3 and C3 treatments with statistically significant differences observed among treatments. In July, the silicon content in stems was 7.06% for S3, 6.04%  for C3, and only 1.94% for the control (B).  By August, significant differences in stem silicon concentration persisted across all treatments, peaking at 10.79% for S3, 8.03% for C3, and 3.69% for the control. In September, the silicon content in stems decreased, with the highest value of 2.1% in S3,  which remained statistically significant higher than in C1 and B. The next highest, C3 at 2.06 %, was also significantly higher than C1 and B. Except for C1, all other treatments showed significant differences only when compared to the control. It is noteworthy that the optimal silicon content optimal for rice stems is 8-10%;  levels below 5% indicate deficiency. Both S3 and C3 treatments were identified as optimal in terms of plant height, stem diameter, number of internodes and internode length.
 Conclusion
The results indicate that as rice plants age and increase in size, silicon concentrations in the stem and leaf decrease, with a more pronounced decline in the leaf. This likely reflects the translocation of silicon from these organs to the husk at the onset of reproductive growth. Among the various treatments, application of 0.24 grams of extracted silicon per 3 kg of soil yielded the greatest improvement in plant growth, followed by commercial siliceous fertilizer. Comparisons between paddy husk ash and rice husk powder treatments revealed that husk ash was more effective for most morphological traits evaluated. Overall, silica addition enhanced rice growth.  Therefore, in paddy soils with silicon concentrations less than 100 mg. kg-1, silicon fertilizer application is recommended. Utilizing silica extracted from rice husk milling by-products can fulfill the silicon requirements of rice fields, providing a sustainable and environmentally friendly source of the essential element of paddy soil fertility management.

کلیدواژه‌ها English

Agricultural residue
Rice husk
Silica fertilizer
Silicon extraction
Agarie, S., Agata, W., Uchida, H., Kubota, F., & Kaufman, P.B. (1996). Function of silica bodies in the epidermal system of rice (Oryza Sativa L.): Testing the window hypothesis. Journal of Experimental   Botany, 47(298(, 655-660. https://doi.org/10.1093/jxb/47.5.655
Agarie, S., Uchida, H., Agata, W.,  Kubota, F., & Kaufamn, B. (1993). Effect of silicon on growth, dry matter production and photosynthesis in rice (Oryza Sativa L.). Crop Production and Improvement Technology, 34, 225-234. https://cir.nii.ac.jp/crid/1571698599899761792
Angelova, D., Uzunov, I., Uzunova, S., Gigova,  A., & Minchev, L. (2011). Kinetics of oil and oil products adsorption by carbonized rice husks. Chemical Engineering Journal, 172(1), 306-311.   https://doi.org/10.1016/j.cej.2011.05.114
Cassman, K.G., Peng, S., Olk, D.D., Ladha, J.K, Reichharolt, W., Dobermann, A., & Singh, U. (1998). Opportunities for increased nitrogen use efficiency from improved resource management in irrigated rice systems. Field Crops Research, 56, 7-39. https://doi.org/10.1016/S0378-4290(97)00140-8
Chakraborty, S., Chowdhury, S., & Das Saha, P. (2011). Adsorption of crystal violet from aqueous solution onto NaOH-modified rice husk. Carbohydrate Polymers, 86(4), 1533-1541. https://doi.org/10.1016/j.carbpol.2011.06.058
Chapman, H.D. (1965). Cation exchange capacity. In: Methods of soil analysis. Part II. Black, C.A. (Ed). American Society of Agronomy, Madison, WI, USA. https://doi.org/10.2134/agronmonogr9.2.c6
Chen, H., Wang, F., Zhang, C., Shi, Y., Jin, G., & Yuan, S. (2010). Preparation of nano-silica materials: The concept from wheat straw. Journal of Non Crystalline Solids, 356(50-51), 2781-2785. https://doi.org/10.1016/j.jnoncrysol.2010.09.051
Chen, W., Yao, X., Cai, K., & Chen, J. (2011). Silicon alleviates drought stress of rice plants by improving  plant water status. Photosynthesis and Mineral Nutrient Research, 142, 67-76. https://doi.org/10.1007/s12011-010-8742-x
Chen, Y .(1990). Characteristics of silicon uptake and accumulation in rice. Journal of Guizhou Agriculture Science, 6, 37–40.
Datnoff, L.E., Deren, C.W., & Snyder, G.H. (1997). Silicon fertilization for disease management of rice in Florida. Crop Protection, 16, 525- 531. https://doi.org/10.1016/S0261-2194(97)00033-1
Dantoff, L.E., Synder, G.H., & Komdorfer, G.H. (2001). Silicon in agriculture. Elsevier, Amsterdam. pp. 285.
Davoudi, M.H., Davaatgar, N., Amiri Larijani, B., Moshiri, F., &  Tehrani, M.M. (2014). Guidelines for integrated soil fertility and plant nutrition management of Rice. Soil and Water Research Institute, 65 page [in Persian].
Day, P.R. (1955). Particle fraction and particle-size analysis. In: Black, C.A. (Ed), Method of soil analysis. Part I. Agronomy 9, Soil Science Society. America. Madison, WI. pp. 545-567.
Doberman, A. (2000). Rice: Nutrient disorders & nutrient management. International Rice Research Institute, Philippines. 203 page.
Dong, L., Yang, T., Ma, L., Li, R., Feng, Y., & Li, Y. (2024). Silicon fertilizer addition can improve rice yield and lodging traits under reduced nitrogen and increased density conditions. Agronomy, 14, 464. https://doi.org/10.3390/agronomy14030464
Ebrahimi Gaskarei, R. (2017). Feasibility and possibility of rice husk availability in rice mill factory in  north of Iran.  Research Report. [In Persian].
Epstein, E. (1991). The anomaly of silicon in plant biology. Proceedings of the National Academy of Sciences of USA, 30, 207.
Fallah, A. (2012). Silicon effect on lodging parameters of rice plants under hydroponic culture . International Journal of Agri Science , 2(7), 630-634.
Food and Agriculture Organization. (2011).
Ghorbani, F., Younesi, H., Mehraban, Z., Sabri, C.M., Ghoreyshi, A.A., & Mansoor, A. (2013). Preparation and characterization of highly pure silica from sedge as agricultural waste and its utilization in the synthesis of mesoporous silica. Journal of Taiwan Institute of Chemical Engineers, 44 (5), 821-828. https://doi.org/10.1016/j.jtice.2013.01.019
Hosseini, S.Z., Babayan Jalodar, N., & Khademi, R. (2009). Effect of silica fertilizer on reduction of damage to chilo suppressalis (Walker) in north rice. National Conference on Water, Soil, Plant and Mechanization of Agriculture. [In Persian].
Jahanshah, S., & Najafi, N. (2011). The role of silicon in the plant. Journal of Olive, 127, 8-17. [In Persian]
Jugal K. Malva ., K. C. Patel., Mohammad Sajid and V. P. Rahmani. (2015). Effect of silicon levels on growth, yield attributes and yield of rice in typic ustochrepts soils. Ecology, Environment and Conservation, 21(August Suppl.), AS205-AS208. https://www.researchgate.net/publication/296906109
Kalapathy, U., Proctor, A., & Shultz, J. (1999). A simple method for production of pure silica from rice hull ash. Bioresource Technology, 73, 257-262. https://doi.org/10.1016/S0960-8524(99)00127-3
Kalra, Y.P. (1998). Reference methods for plant analysis. Soil and plant analysis council, Inc, 300 pages. https://doi.org/10.1016/S0960-8524(99)00127-3
Khader, I., Hashibon, A., Albina, J.M., & Kailer, A. (2011). Wear and corrosion of silicon nitride rolling tools in copper rolling. Wear, 271(9–10), 2531-2541. https://doi.org/10.1016/S0960-8524(99)00127-3
Ma, J.F., & Takahashi, E. (2002). Soil, fertilizer and plant silicon research in Japan. Elsevier. 281p.
Ma, J.F., & Yamaji, N. (2006). Silicon uptake and accumulation in higher plants. Trends in Plant Science, 2(8), 392-397. 10.1016/j.tplants.2006.06.007
Mandlik, R., Thakral, V., Raturi, G., Shinde, S., Nikolić, M., Tripathi, D.K., Sonah, H., & Deshmuk., H.R. (2020). Significance of silicon uptake, transport, and deposition in plants. Journal of  Experimental Botany, 71(21), 6703-6718. https://doi.org/10.1093/jxb/eraa301
Mc Lean, E.O. (1982). Soil pH and lime requirement. P. 192-224. In, Methods of soil analysis (part II) Chemical and microbiological properties, Page et al. (Ed.). American Society of Agronomy, Inc., Soil Science Society of America, Inc. Publisher. Madison, Wisconsin, USA.
Mengel, K. (2001). Principles of plant nutrition. International Potash Institute, 655 pages.
Ministry of Agriculture Jihad. (2015). Agricultural products statistics.
Mobasser, H.R., Ghanbari-Malidareh, A., & Sedghi, A.H. (2008). Effect of silicon application to nitrogen rate and 435plitting on agronomical characteristics of rice (Oryza sativa L.). Silicon in Agriculture, 26-31 .
Motomura, H., Fujii, T., & Suzuki, M. (2000). Distribution of silicified cells in the leaf blades of pleioblastus chino (Franchet et Savatier) Makino (Bambusoideae). Annals of Botany, 85, 751-757. https://doi.org/10.1006/anbo.2000.1124
Nakata, Y., Ueno, M., Kihara, J., Ichii, M., Taketa, S., & Arase, S. (2008). Rice blast disease and susceptibility to pests in a silicon uptake-deficient mutant. Crop Protection, 27, 865-868.    https://doi.org/10.1016/j.cropro.2007.08.016
Park, J.Y., Gu, Y.M., & Chun, J.  (2023). Pilot-scale continuous biogenic silica extraction from rice husk by one-pot alkali hydrothermal treatment and ball milling. Chemical and Biological Technologies in Agriculture, 10, 102. https://doi.org/10.1186/s40538-023-00479-4
Park, J.Y., Gu, Y.M., Park, S.Y., Hwang, E.T., Sang, B.I., Chun, J., & Lee, J.H. (2021). Two-stage continuous process for extraction of silica from rice husk. Using attrition ball milling and alkaline leaching methods. Sustainability, 13, 7350.  https://doi.org/10.3390/su13137350
Ramezani, Z. (2014). Interaction of nitrogen and silicon on growth and nutrient concentrations in two rice cultivars. Master's Thesis. University of Guilan. [In Persian].
Shahdi, A. (2002). Rice research institute, Research Report. Rasht, Iran. [In Persian].
Walkley, A., & Black, I.A. (1934). An examination method for determination of organic matter role and proposed modification of the chromic acid titration method. Soil Science, 37, 29–3.
Yan, G., Huang, Q., Zhao, S., Xu, Y., He, Y., Nikolic, M., Nikolic, N., Liang, Y., & Zhu, Z. (2024). Silicon nanoparticles in sustainable agriculture: synthesis, absorption and plant stress alleviation. Frontiers  in Plant Science,15,1393458. https://doi.org/10.3389/fpls.2024.1393458