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A study on the effects of chemical and biological fertilizers on the essential oil content of sage (Salvia officinalis L.)

Document Type : Research Paper - Medicinal Aromatic Plants

Authors

1 Associate Professor, Research Institute of Forests and Rangelands, Agricultural Research Education and extension Organization (AREEO), Tehran, Iran

2 Expert, Research Institute of Forests and Rangelands, Agricultural Research Education and extension Organization (AREEO), Tehran, Iran

3 Assistant Professor, Research Institute of Forests and Rangelands, Agricultural Research Education and extension Organization (AREEO), Tehran, Iran

4 Assistant Professor, Research Division of Natural Resources, East Azerbaijan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and extension Organization (AREEO), Tabriz, Iran

10.22055/ppd.2024.47372.2188

Abstract

Introduction
The use of medicinal plants and their products has been expanded significantly in various countries. Sage, scientifically known as Salvia officinalis, is a valuable medicinal plant belonging to the mint family. Sage leaves contain various types of flavonoids and phenolic compounds and possess important medicinal properties such as antioxidant and anti-inflammatory activities. Additionally, sage leaves and flowering tops are used in food processing and perfumery. Some compounds present in sage essential oil, such as 1,8-cineole, borneol, camphor, and thujone, exhibit antimicrobial, antioxidant, memory-enhancing, anti-Alzheimer's, and anticancer properties. The application of biofertilizers not only plays a crucial role in increasing the yield of medicinal plants but also affects the quality of active compounds. One of the strategies in sustainable agricultural systems to enhance soil fertility is the minimal use of on-farm inputs, including biofertilizers, and their combined application with organic and chemical fertilizers. Furthermore, nutrient management is a vital factor in successful cultivation.
Materials and methods
A factorial experiment based on a randomized complete block design with three replications was conducted in the 2021-2022 growing season at the Research Farm of the Forest and Rangelands Research Institute of Iran to evaluate the effects of chemical and biological fertilizers on the percentage and composition of sage essential oil. Treatments included a control (no fertilizer), chemical fertilizers (150 kg/ha phosphorus from triple superphosphate and 300 kg/ha nitrogen from urea), and inoculation with mycorrhizal fungi (Funneliformis mosseae and Rhizophagus irregularis) and phosphate-solubilizing bacteria (Pseudomonas fluorescens strain 187).
Results and Discussion
Results showed that the application of chemical fertilizers significantly affected the yield and percentage of essential oil compounds including alpha-pinene, camphene, 1,8-cineole, alpha-thujone, and beta-thujone, as well as the total of hydrocarbon monoterpenes, oxygenated monoterpenes, and oxygenated sesquiterpenes. The yield of compounds such as alpha-pinene, 1,8-cineole, alpha-thujone, beta-thujone, and camphor was also significantly affected. The highest percentage of alpha-thujene (% 0.25) were obtained in the treatment with both urea and phosphate fertilizers. The highest percentage of linalool and camphor yield was obtained in the treatment with biofertilizers. The highest essential oil yield with averages of 97.57, 97.07, and 89.27 kg/ha belonged to the treatments N300P0 * Funneliformis mosseae + Rhizophagus irregularis+ Pseudomonas fluorescens strain 187, Pseudomonas fluorescens strain 187 * N0P150, and Funneliformis mosseae + Rhizophagus irregularis + Pseudomonas fluorescens strain 187* N0P150, respectively."
Conclusion
The results of this study indicated that sage, due to its abundant aerial biomass production, requires adequate fertilization to achieve high yields. However, the essential oil percentage of this plant was not affected by environmental factors such as fertilization and seemed to be more controlled by genetic factors. This finding can significantly contribute to the selection and introduction of cultivars with high essential oil percentages in the future. Furthermore, by combining chemical and biological fertilizers, it is possible to effectively influence the percentage and quantity of major essential oil compounds in sage and achieve the production of higher quality plants with a higher percentage of specific compounds.

Keywords

Main Subjects

References
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Plant Productions
Volume 47, Issue 3
November 2024
Pages 455-474
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