Document Type : Research Paper - Ornamental Plants

Authors

1 M.Sc. Graduate of Horticulture, Department of Horticulture, Faculty of Agriculture, Khorramabad, Iran

2 Associate Professor, Lorestan University, Department of Horticulture, Faculty of Agriculture, Khorramabad, Iran

3 Assistant Professor, Lorestan University, Department of Horticulture, Faculty of Agriculture, Khorramabad, Iran

4 Assistant Professor, Saravan University, Department of Plant medicine, Faculty of Agriculture, Saravan, Iran

Abstract

Introduction
Salinity stress leads to ion toxicity and osmotic stress. Salinity stress through the osmotic mechanism, due to the reduction of the osmotic potential of the soil solution, causes disturbances in transpiration and photosynthesis. The mechanism of action of ionic toxicity is also related to ion absorption and changes in physiological processes caused by toxicity, deficiency or change in the balance of mineral elements. In the last decade, the tendency to use metal nanoparticle compounds in agriculture and horticultural sciences has become popular. Researchers believe that silver nanoparticles are absorbed faster by plants due to their small size and high solubility, therefore, by using these materials, optimal conditions for plant growth are created and stress conditions are prevented in the plant.
Materials and Methods
The experiment was conducted in the greenhouse of Horticultural Science Department, Faculty of Agriculture located in Khorramabad city, Lorestan province in 2021. The experiment was conducted as a factorial in the form of a completely randomized design with four replications. The first factor was included salinity treatment at four levels (0, 25, 50 and 100 mM NaCl, respectively equivalent to 0.067, 2.450, 5.440 and 9.520 dS m-1) and the second factor was foliar application of nanosilver at 4 levels (0, 10, 50 and 100 ppm). Salinity treatments were started gradually with the application of low concentration of salt after establishing the plants in the pot. Nano silver foliar spraying was applied weekly for 5 times until the flowering stage. Foliar spraying with silver nanoparticles was done in 3 stages of four leaves, full growth and flowering.
Results and Discussion
In this study, application of nanosilver increased the quantitative and qualitative characteristics of parsley, including the fresh weight of the flower (by 2.23%). Nanosilver in concentrations of 10 and 50 mg/liter increases the relative content of leaf water (7.70%), the content of total phenol (5.96%), the content of total flavonoids (34.91%) and the amount of potassium in leaves (4.055 %) under salinity stress conditions. Additionally, the lowest amount of sodium leaf (0.162 %), ion leakage (25.44 %) and malondialdehyde (21.56 %) was observed in this treatment. Researchers believe that silver nanoparticles are absorbed faster by plants due to their small size and high solubility, therefore, by using these materials, optimal conditions for plant growth are created and any stressful conditions are prevented in the plant. Silver nanoparticles with suitable size and remarkable chemical stability remain in constant shape and size in solutions.
Conclusion
Based on the results observed in this study, the treatment of silver nanoparticles in concentrations of 10 and 50 ppm in the conditions of salinity stress, relative water content of leaves, potassium content of leaves, fresh and dry weight of flowers and time of budding and flowering were increased, while ion leakage, malondialdehyde, proline, total phenol and flavonoid content, and sodium content of leaves were decreased. Therefore, it can be stated that the application of silver nanoparticles in the form of foliar spraying at levels of 10 and 50 ppm can reduce the negative effects caused by salinity stress and improve the ornamental characteristics of parsley in 25 and 50 mM of salinity stress.
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Allafchian, A.R., Jalali, A.H., Aghaei, F. & Farhang, H.R. (2018)). Green synthesis of silver nanoparticles using Glaucium corniculatum (L.) Curtis extracts andevaluation of its Antibacterial activity. IET Nanobiotechnol, 12 (5), 574-578.
Bates, L.S., Waldren, R.P. & Teare, I.D. (1973). Rapid determination of free proline for water stress studies. Plant Soil J, 39, 205-207.
Buege, J.A. & Aust, S.D. (1978). Microsomal lipid peroxidation. Methods Enzyme, 52, 302-310.
Bybordi, A. (2012). Study effect of salinity on some physiologic and morphologic properties of two grape cultivars. Life Science Journal, 9, 1092-1101.
Chrysargyris, A., Tzionis, A., Xylia, P., & Tzortzakis, N. (2018). Effects of Salinity on Tagetes Growth, Physiology, and Shelf Life of Edible Flowers Stored in Passive Modified Atmosphere Packaging or Treated with Ethanol. Crop and Product Physiology, a section of the journal Frontiers in Plant Science, 9,1765. doi: 10.3389/fpls.2018.01765.
Darvishzadeh, F., Nejatzadeh, F. & Iranbakhsh, A. (2015). The effect of silver nanoparticles on salinity tolerance of basil plants during the germination stages in laboratory conditions. New Journal of Cell-Molecular Biotechnology, 5 (20), 70-63. (In Persian)
Donaldson, K., Oberdörster, G., Maynard, A., Castranova, V., Fitzpatrick, J., Ausman, K., Carter, J., Karn, B., Kreyling, W. & Lai, D. (2005). Principles for characterizing potential human health effects from exposure to nanomaterials: elements of a screening strategy. Particle and Fibre Toxicology, 2 (8).
Funk, V.A., Chan, R. & Holland, A. (2007). Cymbonotus (Compositae: Arctotideae, Arctotidinae): an endemic Australian genus embedded in a southern African clade. Botanical Journal of the Linnean Society, 153(1), 1-8.
Ghasemi, V., Ehtesham Nia, A., Rezaei Nejad, A. & Mumivand, H. (2021). The effect of different levels of salinity stress and cultivar on biochemical and physiological characteristics and nutrient concentration of William Sweet (Dianthus barbatus). Journal of Plant Production, 30 (1), 1-19. DOI: 10.22069/JOPP.2021.19072.2815. (In Persian)
Hasanpouraghdam, M.B., Vojodi Mehraban, L. & Shamsi Khotab, T. (2021). The effect of foliar application of Zinc oxide on some growth charasteristics and elemental concentration of Rosemmary under NaCl salinity. The Plant Production (Scientific Journal of Agriculture), 44 (3), 421-432. (In Persian)
Hassanvand, A., Saadatmand, S., Lari Yazdi, H. & Iranbakhsh, A.R. (2021). The effect of biosynthesized silver nanoparticles on some physiological and biochemical parameters of viola tricolor (Viola tricolor L.). Journal of Plant Environmental Physiology, 62 (16), 109-122. (In Persian)
Isah, T. (2019). Stress and defense responses in plant secondary metabolites production. Biological Research, 52, 39.
Josine, T.L., Ji, J., Wang, G. & Guan, C.F. (2013). Advances in genetic engineering for plants abiotic stress control. African Journal of Biotechnology, 10 (28), 5402-5413.
Kalher, M., Dehestani, M., Shirmardi, M. & Gholam Nejad, J. (2018). The effect of different cultivation media on some physicochemical traits of marigold under salt stress. Plant products (scientific journal of agriculture), 42 (1), 1-12. (In Persian)
Kamali, M., Khosroyar, S. & Jalilvand, M. (2014). Evaluation of phenolic, flavonoids, anthocyanin contents and antioxidant capacities of different extracts of aerial parts of Dracocephalum kotschyi. JNKUMS, 6, 627 -34.
‎Karimi.jafari, A. & Hossein zadeh namil, M. (2020). The effect of salinity and nano silver on growth and biochemistry of saffron corms in soaking conditions. Applied Biology, 29 (1), 159-174. doi: 10.22051/jab.2016.2474
Kaviani, N. & Osfoori, M. (2018). Biological Preparation of Silver Nanoparticles Using Artemisia sieberi. Modar-es Journal of Biotechnology, 9 (1), 23-27.
Khalofah, A., Kilany, M. & Migdadi, H. (2021). Phytostimulatory influence of comamonas testosteroni and silver nanoparticles on Linum usitatissimum L. under salinity stress. Plants, 10 (4), 790.
Khan, M.A., Shirazi, M.U., Khan, M.A., Mujtaba, S.M., Islam, E., Mumtaz, S., Shereen, A., Sharma, V.K., Yngard, R.A. & Lin, Y. (2008). Silver nanoparticles: Green synthesis and their antimicrobial activities. advances in colloid and interface science, 26, 2027-2038.
Khosravian, Z., Ranjbar, M. & Ahadi, A.M. (2022). Investigating the effect of chitosan on gene expression, p5cs enzyme activity, and proline content in rapeseed (Brassica napus L.) under salt stress. Agricultural Biotechnology Journal, 14 (4), 181-200. DOI: 10.22103/jab.2022.18349.1348. (In Persian)
Langroudi, M.E., Hashemabadi, D., KalateJari, S. &Asadpour, L. (2020). Effects of silver nanoparticles, chemical treatments and herbal essential oils on the vase life of cut alstroemeria (Alstroemeria ‘Summer Sky’) flowers. The Journal of Horticultural Science and Biotechnology, 95(2), 175-182.
Lutts, S., Kinet, J.M. & Bouharmont, J. (1996). NaCl-induced senescence inleaves of rice (Oryza sativa L.) cultivars differing in salinitary resistance. Annul of Botany. 78, 3. 389-398.
Mosa, K., Ismail, A. & Ahmed Helmy, M. (2017). Introduction to Plant Stresses. https://doi.org/10.1007%2F978-3-319-59379-1_1.
Najafi, S. & Jamei, R. (2014). Effect of silver nanoparticles and Pb (NO3)2 on the yield and chemical composition of mung bean (Vigna radiata). Journal of Stress Physiology & Biochemistry, 10 (1), 316-325.
Nejatzadeh, F. (2021). Effect of silver nanoparticles on salt tolerance of Satureja hortensis L. during in vitro and in vivo germination tests. Heliyon, 7(2), e05981.
Pourbeyrami Hir, Y., Mehri, S., Chamani, E. & Maleki Lajayer, H. (2021). The effect of silver nanoparticles on morphological and physiological properties of Iris pseudacorus under in vitro conditions. Iranian journal of plant biology, 13 (50), 1-14. (In Persian)
Ritchie S.W. & Hanson A.D. (1990). Leaf water content and gas exchange parameters of two wheat genotypes differing in drought resistance. Crop Science Journal, 30, 105-11.
Romagnoli, C., Mares, D., Fasulo, M.P. & Bruni, A. (2005). Antifungal effects of α‐terthienyl from Tagetes patula on five dermatophytes. Phytotherapy Research, 8(6), 332-336.
Shahraki, H., Mahdi Nezhad, N., Fakheri, B. & Haddadi, F. (2021). The effect of synthesis nanosilver by plant extract on morphological and antioxidant properties of Artichoke under salinity stress. The Plant Production (Scientific Journal of Agriculture), 44 (1), 103-114. (In Persian)
Siahmansour, S., Ehtesham-Nia, A. & Rezaeinejad, A. (2020). Effect of salicylic acid foliar application on Morphophysiological and biochemical traits of Goldenberry (Physalis peruviana L.) under salinity stress condition. J. Plant Production Research. 27, 1. 165-178. (In Persian)
Singleton, V.L., Orthofer, R. & Lamuela-Raventos, R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 152–178. https://doi.org/10.1016/S0076-6879(99)99017-1.
Solgi, M. & Taghizadeh, M. (2017). The Effects of Silver Nitrate, Thymol, Green Silver Nanoparticles and Chitosan on Vase Life of Carnation Cut Flowers cv. White Liberty. The Plant Production (Scientific Journal of Agriculture), 40 (2), 1-12. (In Persian)
Summart, J., Thanonkeo, P., Panichajakul, S., Prathepha, P. & McManus, M.T. (2010). Effect of salt stress on growth, inorganic ion and proline accumulation in (Thai aromatic) rice, Khao Dawk Mali 105, callus culture. African journal of Biotechnology, 9, 145-152.
Wahing, I., Van, W., Houba, V.J.G. & Vander, J.J. (1989). Soil and plant analysis a series of syllabi. Plant analysis procedure. Wageningen agriculture university.
Yin, L., Wang, S., Li, J., Tanaka, K. & Oka, M. (2013). Application of silicon improves salt tolerance through ameliorating osmotic and ionic stresses in the seedling of Sorghum bicolor. Acta Physiology Plant, 35, 3099-3107.
Zhang, G., Wang, Y., Wu, K., Zhang, Q., Feng, Y., Miao, Y. & Yan, Z. (2021). Exogenous application of chitosan alleviates salinity stress in lettuce (Lactuca sativa L.). Horticulturae, 7, 342. doi.org/10.3390/horticulturae7100342.