Document Type : Research Paper - Post Harvest Physiology

Authors

1 MSc student of Horticulture, Department of Horticulture, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

2 Associate Professor, Department of Horticulture, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

3 Assistant Professor, Department of Horticulture, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

Abstract

Introduction
Food safety is one of the important issues related to fresh fruits and vegetables. Most natural compounds are degradable and can be a safe compound for human health and the environment and an alternative to synthetic compounds. Research has shown that the combination of bioactive substances such as essential oils, plant extracts and nanocomposites increases the active effect of the coating as fruit packaging and reduces the rate of fruit deterioration.
 
Materials and Methods
In this research, physalis fruits were obtained from Khorramabad research greenhouse. Nanocellulose and pure carvacrol (extracted from Satureja khuzistanica Jamza ( were prepared from Nanovin Nano Polymer Company and GC/MS analysis from Khorramabad Pharmaceutical Company, respectively. Also, the combination of nanocellulose with carvacrol was prepared in the laboratory. The experiment was conducted as a factorial experiment with two factors in a completely randomized design with 4 replications. The first factor of material type in specific concentrations at nine levels (control, Car 0.3%, Car 0.6%, CNF 0.5%, CNF 1.5%, Car 0.3 + CNF 0.5%, Car 0.3 + CNF 1.5%, Car 0.6 + CNF 0.5 %, Car 0.6+CNF 1.5% and the second storage time factor was investigated at four levels (0, 45, 90 and 135 days). First, the fruits of Physalis were washed with distilled water, and then the extra leaves and damaged fruits were removed. The fruits were placed in the prepared solution for 2-3 minutes and after applying the treatment, the fruits were placed in a refrigerator with a temperature of 4 °and 90% RH.
 Results and Discussion
The results showed that the fruits treated with nanocellulose and carvacrol had less weight loss and higher tissue firmness. Byochemiucal analysis also showed the positive effect of the treatments on parameters, so that the highest TA and vitamin C and the lowest amount of pH were obtained in the combined treatments of nanocellulose and nanocellulose/carvacrol. Also, the treatments used reduced the TSS and fruit TSS/TA ratio of the fruits, and the combined treatments had the lowest amount. The nanocellulose coating prevents water loss and reduces the metabolic and respiration processes. Also, nanocellulose/carvacrol composite coatings lead to an increase in the pH of cytochrome oxidase by reducing internal oxygen, and this enzyme increases the rate of ascorbic acid decomposition. By coating on the fruit during the post-harvest period, the aging process of the fruit is reduced the production and effect of ethylene, reducing respiration and preserving the cell wall, which ultimately leads to better preservation of TSS. Therefore, any reduction in metabolic activities causes a reduction in fruit ethylene biosynthesis and action.
 
Conclusion
In general, the results of the research showed that the investigated characteristics of physalis fruit were influenced by the treatments. As treated samples, weight loss was less, pH, soluble solids and taste index were lower, tissue firmness, titratable acids and vitamin C were higher. According to the general results, it can be stated that the application of nanocellulose/carvacrol composite treatments, especially the Car 0.6+CNF 1.5% treatment, showed more favorable results than the application of each one alone in most features.
.

Keywords

Main Subjects

Acevedo-Fani, A., Salvia-Trujillo, L., Alejandra Rojas Graü, M. & Martín-Belloso, O. (2015). Edible films from essential oil-loaded nanoemulsions: Pاysicochemical characterization and antimicrobial properties. Food Hydrocolloids, 47, 168-177.
Ahmadi Soleimanie, S. & Vafaee, Y. (2018). Storability and postharvest quality of five Iranian grape cultivars during cold storage. Plant Physiology Reports, 3(21), 1-12.
Alizadeh, A., Khoshkhui, M., Javidnia, K. & Firuzi, O.R. (2010). Essential oil composition of three medicinal plants from labiatae family. The First National Symposium on Agriculture and Sustainable Development Opportunities and Future Challenges. Conference of Shiraz Branch, Islamic Azad University. Shiraz, Iran.
Amorati, R., Foti, M.C. & Valgimigli, L. (2013). Antioxidant activity of essential oils. Journal of Agriculture Food Chemistry, 61, 10835–10847.
Cardenas-Barboza, L. C., Paredes-Cordoba, A. C., Serna-Cock, L., Guancha-Chalapud, M. and Torres-Leon, C. (2021). Quality of Physalis peruviana fruits coated with pectin and pectin reinforced with nanocellulose from P. peruviana calyces. Heliyon7(9), 441-455.
Cosme Silva, G. M., Silva, W. B., Medeiros, D. B., Salvador, A. R., Cordeiro, M. H. M., da Silva, N. M. and Mizobutsi, G. P. (2017). The chitosan affects severely the carbon metabolism in mango (Mangifera indica L. cv. Palmer) fruit during storage. Food Chemistry, 237, 372–378.
Diaz-Mula, H.M., Serrano, M. and Valero, D. (2012). Alginate Coatings Preserve Fruit Quality and Bioactive Compounds during Storage of Sweet Cherry Fruit. Food Bioprocess Technology, 5, 2990–2997.
Duan, J., Wu, R., Stric, B. C. & Zhao, Y. (2011). Effect of edible coatings of fresh blueberries (Duke and Elliott) under commercial storage conditions. Postharvest Biology and Technology, 59(1), 71–79.
Duduk, N., Markovic, T., Vasic, M., Duduk, B., Vico, I. and Obradovic, A. (2015). Antifungal Activity of Three Essential Oils against Colletotrichum acutatum, the Causal Agent of Strawberry Anthracnose. Journal of Essential Oil Bearing Plant, 18, 529–537.
Ehtesham Nia, A., Taghipour, S., & Siahmansour, S. (2021). Pre-harvest application of chitosan and postharvest Aloe vera gel coating enhances quality of table grape (Vitis vinifera L. cv. ‘Yaghouti’) during postharvest period. Food Chemistry, 347, 129012.
Eshghi, S., Hashemi, M., Mohammadi, A., Badii, F., Mohammadhoseini, Z. & Ahmadi, K. (2014). Effect of nanochitosan -based coating with and without copper loaded on pHysicochemical and bioactive components of fresh strawberry fruit (Fragaria x ananassa Duchesne) during storage. Food and Bioprocess Technology, 7 (8), 2397 -2409.
Fischer, G. (2000). In Productión, poscosecha y exportatión goldenberry (PHysalis peruviana L.). Unibiblos: Universidad Nacional de Colombia, p. 9-26.
Gao, P. S., Zhu, Z. Q. & Zhang, P. (2013). Effects of chitosan-glucose complex coating on postharvest quality and shelf life of table grapes. Carbohydrate Polymers, 95, 371–378.
Guerra, I. C. D., de Oliveira, P. D. L., de Souza Pontes, A. L., Lucio, A. S. S. C., Tavares, J. F., Barbosa-Filho, J. M. & de Souza, E. L. (2015). Coatings comprising chitosan and Mentha piperita L. or Mentha × villosa Huds essential oils to prevent common postharvest mold infections and maintain the quality of cherry tomato fruit. International Journal of Food Microbiology, 214, 168–178.
Hassan, B., Chatha, S. A. S., Hussain, A. I., Zia, K. M. & Akhtar, N. (2017). Recent advances on polysaccharides, lipids and protein based edible films and coatings: A review. International journal of biological macromolecules, 109, 1095-1107.
Heydar Nejad, R., Ghahremani, Z., barzegar, T. & Rabiei, V. (2017). The effect of harvest stage and storage duration on fruit quality of Physalis (Physalis angulate L.). Journal of Agricultural Crops Production, 20(2), 383-394.
Hocking, B., Tyerman, S.D., Burton, R.A. & Gilliham, M. (2016). Fruit Calcium: Transport and PHysiology. Front Plant Science. 7, 569.
Hong, K., Xie, J., Zhang, L., Sun, D., Gong, D., Hossain, M.S. & Iqbal, A. (2016) Effect of shrimp chitosan coating on postharvest quality of banana fruit. International Food Research Journal, 23(3),277–283.
Hussain, A., Rizwan, M., Ali, Q. & Ali, S. (2019). Seed priming with silicon nanoparticles improved the biomass and yield while reduced the oxidative stress and cadmium concentration in wheat grains. Environment Science Pollutant Research, 26, 7579–7588.
Morton, J. (2010). Cape Gooseberry: Physalis peruviana L.; Physalis edulis Sims. Fruits of warm climates. Julia F. Morton: Miami Fl., 430-434.
Najafi Marghmaleki, S., Mortazavi, S. M. H. & Motamedi, H. (2020). The Study of Changes in Phytochemical Compounds of Date Fruit cv. Barhee During Development and Ripening. Plant Productions, 42(4), 563-574. [In Persian]
Pek, Z. & Helyes, L. (2010). Color changes and antioxidant content of vine and postharvest ripened tomato fruits. Horticultural science, 45(3), 466 –468.
Pirozzi, A., Ferrari, G. & Donsì, F. (2021). The use of nanocellulose in edible coatings for the preservation of perishable fruits and vegetables. Coatings, 11(8), 990.
Pizato, S., Sebastian Vega-Herrera, S., Costa Chevalier, R., Arevalo Pinedo, R. & Renzo Cortez-Vega, W. (2022). Impact of Chitosan Coatings Enriched with Clove Essential Oil on Quality of Minimally Processed Strawberries. Food/Feed Science and Technology, 65, e22210278.
Rapisarda, P., Bianco, L., Pannuzzo, P. & Timpanaro, N. (2008). Effect of cold storage on vitamin C, pHenolic and antioxidant activity of five orange genotype (Citrus sineniss L. osbeck). Postharvest Biology and Technology, 49, 346-354.
Salvia-Trujillo, L., Rojas-Grau, M.A., Soliva-Fortuny, R. & Martin-Belloso, O. (2015). Use of antimicrobial nanoemulsions as edible coatings: Impact on safety and quality attributes of fresh-cut Fuji apples. Postharvest Biology and Technology, 105, 8–16.
Silva, E. L. P., Carvalho, T. C. & Ayub, R. A. (2020). Blackberry extend shelf life by nanocellulose and vegetable oil coating. Horticultural International Journal, 4(2), 54-60.
Sogvar, O.B., Koushesh Saba, M. & Emamifar, A. (2016). Aloe vera and ascorbic acid coatings maintain postharvest quality and reduce microbial load of strawberry fruit. Postharvest Biology and Technology. 114, 29–35.
Steelheart, C., Alegre, M.L., Bahima, J.V., Senn, M.E., Simontacchi, M., Bartoli, C.G. & Grozeff, G.E. (2019). Nitric oxide improves the effect of 1-methylcyclopropene extending the tomato (Lycopersicum esculentum L.) fruit postharvest life. Science Horticulture, 255, 193–201.
Trevino-Garza, M.Z., Garcia, S., Flores-Gonzalez, M.D.S. & Arevalo-Nino, K. (2015). Edible active coatings based on pectin, pullulan, and chitosan increase quality and shelf life of strawberries (Fragaria ananassa). Journal of Food Science, 80, 1823–1830.
Vojodi Mehrabani, L., Valizadeh Kamran, R. & Bagher Hassanpouraghdam, M. (2019). Evaluation of Some Phytochemical Characteristics of Berberis integerrima in Response to Nano-Zinc Foliar Application and Post-Harvest Drying Temperature. Plant Productions, 42(3), 345-358. [In Persian]
Xiao, J., Gu, C., Zhu, D., Huang, Y., Luo, Y. & Zhou, Q. (2021). Development and characterization of an edible chitosan/zein-cinnamaldehyde nano-cellulose composite film and its effects on mango quality during storage. LWT, 140, 325-337. 110809.
Yadav, G.D. & Kamble, S.B. (2009). Synthesis of carvacrol by Friedel–Crafts alkylation of ocresol with isopropanol using super acidic catalyst UDCaT-5. Journal of Chemical Technology and Biology, 84, 1499-1508.