Plant Productions

Plant Productions

Investigation of Seed Storage Proteins Diversity in Some Durum Wheat Landraces and Cultivars

Document Type : Research Paper - Agronomy

Authors
Fatemeh Soorninia 1
Abdollah Najaphy 2
Danial Kahrizi 3
Ali Mostafaie 4
Ali Ashraf Mehrabi 5
1 Ph.D. Student, Department of Plant Production and Genetics, Faculty of Agricultural Sciences and Engineering, Razi University, Kermanshah, Iran
2 Associate Professor, Department of Plant Production and Genetics, Faculty of Agricultural Sciences and Engineering, Razi University, Kermanshah, Iran
3 Professor, Department of Plant Production and Genetics, Faculty of Agricultural Sciences and Engineering, Razi University, Kermanshah, Iran
4 Professor, School of Medicine, Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
5 Biotechnology Research Division, Associate Professor, Research Institute of Forests and Rangelands, Iran
10.22055/ppd.2022.39915.2012
Abstract
Introduction
Reducing the genetic diversity of crops as a result of the use of breeding processes leads to the loss of a large number of useful genes and thus reduces the diversity of genetic resources, which is one of the undeniable tools of breeding programs. Therefore, researches aimed at studying the genetic diversity of crops can be an effective aid in preserving genetic resources and identifying superior genotypes. Electrophoresis of seed storage proteins is a useful tool to study the characteristics of wheat genotypes in terms of storage proteins diversity.
 
Materials and Methods
In the present study, the diversity of seed storage proteins of 25 durum wheat genotypes obtained from different regions of Iran, including some landraces and commercial cultivars, was investigated using SDS-PAGE electrophoresis. Storage proteins of the studied genotypes were scored and analyzed after extraction and electrophoresis. Grain protein content and total gluten were measured by NIR and glutomatic gluten washer machines, respectively.
 
 
Results and Discussion
Electrophoresis identified 14 polymorphic bands. Molecular analysis of variance between landraces and commercial cultivars showed 100% intragroup diversity. Cluster analysis using Jaccard coefficients by UPGMA method divided the studied genotypes into 4 groups. Genotypes No. 9 (Land race of Mashhad), 18 (Land race of Ahar) and 24 (Landrace of Islamabad Gharb) were placed each in a separate group and the rest of the genotypes formed another group. The two-dimensional graph resulting from principal component analysis also confirmed these four groups. Based on Jaccard similarity matrix, the lowest similarity and highest genetic distance were determined between genotypes No. 24 (Land race of Islamabad Gharb) with genotypes No. 21 (Dehdasht cultivar), 22 (Land race of Khorramabad), 23 (Dena cultivar) and 25 (Land race of Khorramabad) with a similarity coefficient of 0.41 was obtained. On the other hand, comparing the mean of genotypes for qualitative traits of grain protein content and total gluten, the highest amount of these two traits for genotypes No. 3 (Landrace of Kermanshah), 4 (Landrace of unknown-Iran), 1 (Landrace of Khoram Abad) and 2 (Landrace of Khoramabad) and the lowest values of both traits were obtained for genotype No. 24 (Landrace of Islamabad-West). Genotype No. 18 (Landrace of Ahar) is also one of the genotypes with high amounts of gluten.
 
Conclusion
The electrophoretic pattern of seed storage proteins can determine genetic diversity based on the presence or absence of protein bands. Therefore it can be a useful tool to identify the genetic diversity of different plants. It is also possible for genotypes that have the greatest genetic distance and at the same time have good quantitative and qualitative traits to participate in breeding programs as parents. In the present study, according to the study of genotypes by analyzing the variance of qualitative traits of grain protein content and total gluten and comparing the mean and grouping them using the information obtained by electrophoresis, a cross can be found between genotypes genotypes No. 3 (Landrace of Kermanshah), 4 (Landrace of unknown-Iran), 1 (Landrace of Khoram Abad) and 2 (Landrace of Khoramabad) Due to their high content of gluten and protein with genotype No. 18 (Landrace of Ahar) due to its high content of gluten and acceptable genetic distance with the mentioned genotypes
Keywords
Cluster analysis
Electrophoresis
Genetic diversity
Principal component analysis
Subjects
Abebe, T. D., Mathew, B., & Léon, J. (2013). Barrier analysis detected genetic discontinuity among Ethiopian barley (Hordeum vulgare L.) landraces due to landscape and human mobility on gene flow. Genetic Resources and Crop Evolution, 60, 297-309.
Ahmadpoor, F., Asghari-Zakaria, R., Firoozi, B., Shahbazi, H., & Sofalian, O. (2015). Genetic diversity in ecotypes of Aegilops biuncialis for seed storage proteins. Modern Genetic Journal, 10(2), 239-250. [In Persian]
Alvarez, J. B., & Guzmán, C. (2019). Recovery of wheat heritage for traditional food: Genetic variation for high molecular weight glutenin subunits in neglected/underutilized wheat. Agronomy, 9(11), 755.
Bhandari, H., Bhanu, A. N., Srivastava, K., Singh, M., & Shreya, H. A. (2017). Assessment of genetic diversity in crop plants-an overview. Advances in Plants & Agriculture Research, 7(3), 279-286.
Canada, A. A. F. (2019). Canada: Outlook for principal field crops. 19 July 2019. Available online: http://www.agr.gc.ca/eng/industry-markets-and-trade/canadian-agri-food-sector-intelligence/crops/reports-and-statistics-data-for-canadian-principal-field-crops/.1378743094676 (accessed on 26 September 2019).
Chacón, E. A., Vázquez, F. J., Giraldo, P., Carrillo, J. M., Benavente, E., & Rodriguez-Quijano, M. (2020). Allelic variation for prolamins in Spanish durum wheat landraces and its relationship with quality traits. Agronomy, 10(1), 136.
Chňapek, M., Tomka, M., Gálová, Z., & Balážová, Ž. (2021). Utilization of storage proteins polymorphism for differentiation of wheat, rye and triticale genotypes. Journal of Microbiology, Biotechnology and Food Sciences, 2(1), 2087-2098.
Cornish, G., Bekes, F., Allen, H., & Martin, D. (2001). Flour proteins linked to quality traits in an Australian doubled haploid wheat population. Australian Journal of Agricultural Research, 52(12), 1339-1348.
El-Beltagi, H. S., Basit, A., Ullah, I. Z., Ullah, In., Mohamed, H. I., & Alqurashi, R. M. (2021). Assessment of biochemical diversity, estimation of mineral composition and nutritional status in different wheat (Triticum aestivum L.) genotypes. Fresenius Environmental Bulletin, 30(7), 9490-9507.
Fathalipoor, Z., Nabati Ahmadi, D., Rajabi Memari, H., Siahpoosh, A., &Sedighi Dehkordi, F. (2015). Determination of plant diversity using morphological characters and path analysis in dill germplasms. Plant Productions, 37(4), 57-67 .[In Persian]
Fathi, T., Souhani, M. M., Lahiji, H., & Mehrabi, A. A. (2014). Genetic diversity among accessions of Aegilops triunsialis L. from west of Iran using ISSR molecular markers. Iranian Journal of Field Crop Science, 45(1), 85- 93 .[In Persian]
José, M. R. J. F. V., & Carrillo, M. (2005). Genetic bases of grain quality. In Durum wheat breeding (pp. 381-408). Boca Raton, Florida: CRC Press.
Kakaei, M., & Kahrizi, D. (2011). Evaluation of seed storage protein patterns of ten wheat varieties using SDS-PAGE. Biharean Biologist, 5(2), 116-118.
Kakaei, M., Mordi, F., & Shararbar, H. (2016). Study of Endosperm Protein Banding Pattern Variation in some of Wheat (Triticum aestivum L.) Genotypes by SDS-PAGE Method. Agricultural Biotechnology, 7(2),77-83. [In Persian]
Khan, M. R., Anjum, F. M., Pasha, I., Shabbir, M. A., Hussain, S., & Nadeem, M. (2012). Application of enzyme-linked immunosorbent assay for the assessment of spring wheat quality. Food and Agricultural Immunology, 23(1), 1-15.
Marone, D., Russo, M. A., Mores, A., Ficco, D. B., Laidò, G., Mastrangelo, A. M., & Borrelli, G. M. (2021). Importance of landraces in cereal breeding for stress tolerance. Plants, 10(7), 1267.
Mehrabi, A., Mohammadi, S., Valian, S., & Khosroshahly, M. (2015). Genetic diversity of Aegilops triuncialis L. accessions of Iran revealed by microsatellite markers. Iranian Journal of Field Crop Scence, 46(2), 237-245. [In Persian]
Mohammadi, R., Abdolahi, A., Mohammadi, M., Elahi, K., & Yari, S. (2016). Evaluation of yield gap of durum wheat genotypes under research and farmers' fields conditions. Research Achievements for Field and Horticulture Crops, 5(2), 133-141. [In Persian]
Nadeem, M., Anjum, F.M., Khan, M.R., Sajjad, M., Hussain, S., & Arshad, M. S. (2016). Electrophoretic characteristics of gluten proteins as influenced by crop year and variety. International Journal of Food Properties, 19(4), 897-910.
Najafian, G., & Baghaie, N. (2011). Genetic variation in high molecular weight glutenin subunits in parental lines and cultivars of wheat used in breeding programs of cold and temperate agro-climatic zones of Iran. Seed and Plant Improvement Journal, 27(3), 305-322. [In Persian]
Nazco, R., Peña, R., Ammar, K., Villegas, D., Crossa, J., Moragues, M.,  & Royo, C. (2014). Variability in glutenin subunit composition of Mediterranean durum wheat germplasm and its relationship with gluten strength. The Journal of Agricultural Science, 152(3), 379-393.
Osmani, Z., & Siosemardeh, A. (2009). A study of genetic diversity in Sardari wheat ecotypes using AFLP markers and agronomic traits. Journal of Science and Technology of Agriculture and Natural Resources, 13(47), 301-320. [In Persian]
Rajabi Hashtjin, M., Fotukian, M. H., Aqaei, M., & Mohammadi, M. (2014). Evaluation of allelic variation and assessment of quality of seed storage proteins in durum wheats. Agricultural Biotechnology Journal, 5(4), 17-36. [In Persian]
Razavizadeh, R., & Ehsanpour, A. (2013). Application of seed storage protein marker for identification of seven soybean (Glycine max) cultivars. Journal of Cell & Tissue, 3(4), 319-326. [In Persian]
Seyedi, N., Jalali, S. G. A., Moghaddam, M., Tabari, M., &Mohammadi, S. A. (2011). Application of seed storage protein in intra-specific variation in three population of Pistacia atlantica Desf. Iranian Journal of Plant Biology, 2(6), 1-14. [In Persian]
Shayan, S., Moghaddam Vahed, M., Mohammadi, S. A., Ghassemi Golezani, K., Sadeghpour, F., & Youssefi, A. (2020). Genetic Diversity and Grouping of Winter Barley Genotypes for Root Characteristics and ISSR Markers. Plant Productions, 43(3), 323-336. [In Persian]
Sihmar, M., Sharma, J. K., Santal, A. R., & Singh, N. (2020). Seed storage protein phylogenetics of Indian wheat genotypes belong to Triticum aestivum, T. dicoccum and T. durum. Indian Journal of Biotechnology, 19, 17-27.
Sissons, M. (2008). Role of durum wheat composition on the quality of pasta and bread. Food, 2(2), 75-90.
Subira, J., Peña, R.J., Álvaro, F., Ammar, K., Ramdani, A., & Royo, C. (2014). Breeding progress in the pasta-making quality of durum wheat cultivars released in Italy and Spain during the 20th Century. Crop Pasture Sciences, 65(1), 16–26.
Vida, G., Szunics, L., Veisz, O., Bedo, Z., Láng, L., Arendas, T., Bónis, P., & Rakszegi, M. (2014). Effect of genotypic, meteorological and agronomic factors on the gluten index of winter durum wheat. Euphytica, 197(1), 61-71.
Weising, K., Nybom, H., Wolff, K., & Kahl, G. (2005). DNA fingerprinting in plants principles, methods, and applications second edition. Boca Raton, Florida: CRC Press.
Xynias, I. N., Mylonas, I., Korpetis, E. G., Ninou, E., Tsaballa, A., Avdikos, I. D., & Mavromatis, A.G. (2020). Durum wheat breeding in the Mediterranean region: Current status and future prospects. Agronomy, 10(3), 432.
Zhu, P., Saadati, H., & Khayatnezhad, M. (2021). Application of probability decision system and particle swarm optimization for improving soil moisture content. Water Supply, 21(8), 4145-4152.
Plant Productions
Volume 45, Issue 3
Autumn 2022
Pages 385-396