نوع مقاله : علمی - پژوهشی

نویسندگان

1 تولیدات گیاهی ، مرکز آموزش عالی کشاورزی بردسیر- دانشگاه شهید باهنر کرمان

2 گروه تولیدات گیاهی، مرکز آموزش عالی کشاورزی بردسیر- دانشگاه شهید باهنر کرمان

چکیده

به این منظور، آزمایشی گلخانه‌ای به صورت فاکتوریل بر پایه طرح بلوک های کامل تصادفی و با چهار تکرار در گلخانه آموزشی پژوهشی دانشکده کشاورزی دانشگاه ولیعصر رفسنجان در سال زراعی 1397 اجرا شد. فاکتورهای آزمایش شامل چهار سطح مختلف تنش شوری خاک (صفر، 6، 12 و 18 دسی‌زیمنس بر متر) و کادمیوم (صفر، 1، 5 و 10 میلی‌گرم بر کیلوگرم خاک) بود. نتایج نشان داد صفات وزن خشک شاخه‌های جانبی، وزن خشک برگ و ساقه و سطح برگ، محتوای نسبی آب برگ، میزان کلروفیل a، کلروفیل b، کلروفیل کل، کاروتنوئید، غلظت آهن، روی، کلسیم و منیزیم برگ با افزایش شوری به 18 دسی زیمنس بر متر به ترتیب 8/56، 01/48، 50، 09/46، 21، 76/53، 91/56، 75/53، 21/51، 9/07،5/73، 8/51، 17/0 و 26/0 درصد کاهش یافتند. با افزایش غلظت کادمیوم از صفر به 10 میلی‌گرم بر کیلوگرم خاک وزن خشک شاخه‌های جانبی، برگ، ساقه، سطح برگ، غلظت روی و منیزیم به ترتیب 32/24، 89/31، 45/28، 24/41، 70/28 و 12/0 درصد کاهش یافتند اما غلظت کادمیوم برگ نسبت به تیمار شاهد 7/1 برابر و غلظت آهن برگ 3/37 درصد افزایش یافتند. میزان فعالیت آنزیم‌های سوپراکسید دسموتاز، پراکسیداز، کاتالاز تحت اثرمتقابل معنی دار تنش شوری و کادمیوم قرار گرفتند و فعالیت آنها در تیمار تنش همزمان شوری 18 دسی زیمنس بر متر و غلظت 10 میلی‌گرم بر کیلوگرم خاک کادمیوم به ترتیب 83/1، 4/1 و 7/4 برابر تیمار شاهد بود. ب

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Evaluation of growth and physiological reactions of Kochia plant to simultaneous stress of salinity and cadmium

نویسندگان [English]

  • mohadeseh shamsaddin saied 1
  • nasibe Pour ghasemian 2

1 Assistant Professor, Department of Plant Productions, Agricultural Faculty of Bardsir, Shahid Bahonar University of Kerman,kerman, Iran

2 Assistant Professor, Department of Plant Productions, Agricultural Faculty of Bardsir, Shahid Bahonar University of Kerman, Kerman, Iran

چکیده [English]

Evaluation of growth and physiological reactions of Kochia plant to simultaneous stress of salinity and cadmium

Background and Objectives: Nowadayes, due to the increasing use of phosphorus fertilizers in agricultural lands, the salinity of the soil along with contamination with heavy elements such as cadmium has increased sharply. Plants' response to a particular stress is different from when they experience multiple stresses simultaneously. The aim of this experiment was to investigate the simultaneous effect of salinity and cadmium stresses on morphological and physiological characteristics of Kochia.

Materials and Methods: For this purpose, a factorial experiment based on a completely randomized design with four replications was conducted in the greenhouse of Bardsir Agricultural Higher Education Center in Shahid Bahonar University of Kerman in 1397. Experimental factors included four different levels of salinity stress (0, 6, 12 and 18 dS / m) and cadmium concentration in soil (0, 1, 5 and 10 mg / kg soil). The studied traits included dry weight of branches, stems and leaves, RWC, concentration of chlorophyll a, b and a + b, enzymes activity of superoxide dismutase, peroxidase, catalase, the concentration of cadmium, iron, zinc, manganese, calcium and magnesium.

Background and Objectives

Nowadayes, due to the increasing use of phosphorus fertilizers in agricultural lands, the salinity of the soil along with contamination with heavy elements such as cadmium has increased sharply. Plants' response to a particular stress is different from when they experience multiple stresses simultaneously. The aim of this experiment was to investigate the simultaneous effect of salinity and cadmium stresses on morphological and physiological characteristics of Kochia.

Materials and Methods

For this purpose, a factorial greenhouse experiment based on a randomized complete block design with four replications was conducted in the educational-research greenhouse of the Faculty of Agriculture, Valiasr University of Rafsanjan in 1397. Experimental factors included four different levels of salinity stress (0, 6, 12 and 18 dS/m) and cadmium concentration in soil (0, 1, 5 and 10 mg/kg soil). The studied traits included dry weight of branches, stems and leaves, RWC, concentration of chlorophyll a, b and a + b, enzymes activity of superoxide dismutase, peroxidase, catalase, the concentration of cadmium, iron, zinc, manganese, calcium and magnesium.

Results

The results showed that due to increasing salinity to 18 dS/m the dry weight of branches, leaf and stem dry weight and leaf area, RWC, concentration of chlorophyll a, chlorophyll b, total chlorophyll and carotenoids decreased by 56.8, 48.01, 50, 46.09, 21, 53.76, 56.91, 53.75 and 51.21 percent, respectively. By increasing the cadmium concentration to 10 mg/L, the dry weight of lateral branches, leaves, stems and leaf area decreased by 24.32, 31.89, 28.45 and 41.24%, respectively, but the concentration of leaf cadmium compared to the control treatment1.7 times was increased. The activity of superoxide dismutase, peroxidase and catalase enzymes in treatment of 18 dS/m salinity alongside with 10 mg/L cadmium were 1.83, 1.4 and 4.7 times higher than the control treatment, respectively. Leaf iron, zinc, calcium and magnesium concentrations decreased by 73.07%, 51.51, 51.8%, 0.17 and 0.26% with increasing salinity stress to 18 dS/m, respectively, and with increasing cadmium concentration to 10 mg/L increased iron concentration by 37.32% and zinc and magnesium concentrations decreased by 28.70 and 0.12%, respectively.

Discussion

The highest dry matter of Koshia in salinity and cadmium stress treatments belonged to the control treatment and the lowest dry matter were obtained from 18 dS / m salinity and 10 mg / kg cadmium in soil.

Keywords: Dry weight, leaf area, antioxidant enzymes, chlorophyll, concentration of elements.



Discussion: Considering the acceptable growth of Koshia plant under salinity stress and the invisible effects of

کلیدواژه‌ها [English]

  • dry weight
  • Leaf area
  • Antioxidant enzymes
  • Chlorophyll
  • concentration of elements