عنوان مقاله [English]
Background and Objectives
Ocimum basilicum L. (O. basilicum) or sweet basil belongs to the family Lamiaceae which has been used as a medicinal plant and its essential oils are used to flavor foods, in dental and oral products and in fragrances. One of the methods to increase yield and availability of minerals in the soil, with the decreased use of inorganic fertilizers, is to apply biological fertilization, which also affects the essential oil of aromatic plants. Hence, this study was carried out to describe detailed growth and chemical investigation of basil essential oil affected by chemical and biological fertilization.
Materials and Methods
This study was carried out as factorial based on completely randomized design with three replications. The first factor involved plant (O. basilicum var. Thyrsiflora and O. sanctum.L ) and the second factor was fertilizer types (0%, 50% and 100% of the plant phosphorus requirement, only bacteria, bacteria with 50% of plant phosphorus requirement, bacteria with 100% of plant phosphorus requirement). The pots filled with five kilograms of soil. Phosphorus fertilizer was added to the pots from the triple super phosphate source based on the experimental treatments. Overnight culture of Pseudomonas putida in nutrient broth was prepared and applied under each seed. At full bloom stage, the leaves and inflorescences of each plant were completely separated and their number counted. Extraction of leaf samples was performed using 70% methanol solvent to measure phenolic compounds and the flavon and flavonol content of the extract was read by measuring the absorbance at 425 nm. The amount of phosphorus content of plant leave was also determined.
Shoot dry weight, number and length of inflorescence were significantly (p