Document Type : English Articles

Author

Assistant Professor, Department of Soil Sciences, College of Agriculture and Natural Resources, Lorestan University, Khorramabad, Iran

Abstract

ABSTRACT
Introduction:Supplying a sufficient amount of available iron (Fe) for plant growth in hydroponic nutrient solutions is a great challenge. There are various Fe fertilizers to eliminate iron deficiency in crops. The chelators commonly used to supply Fe in nutrient solutions have several disadvantages and may negatively affect plant growth. The objective of the current paper was to evaluate the effects of some various Fe- chelates, (Fe-arginine, Fe-glycine, Fe-histidine, Fe-lysine, Fe-glutamine and Fe-EDTA) on the physiological properties and nutraceutical potential of soybean (Glycine max (L.) Merr.) grown in nutrient solution.
Materials and Methods:The experiments had a randomized complete block design with three replications and the treatments were arranged in factorial combination. The plants were grown in a greenhouse of Faculty of Agriculture at Lorestan University in 2018. In this study, Fe- chelates were synthesized. Then, soybean seeds were sterilized and germinated in 11 μm filter paper. Seven-day seedlings were transplanted into perlite and watered regularly with distilled water for a week. Then, Two-pair leaflet seedlings were placed in continuously aerated solution under controlled conditions of the greenhouse. Six different iron treatments (at Fe level=50 µM) were applied including: Fe-EDTA, Fe-arginine, Fe-Glycine, Fe-histidine, Fe-glutamine, and Fe-lysine. The plant leaves were collected at 10-leaves stages. The seed collection was done in the maturity of harvest (R8). Afterwards, physiological parameters, antioxidant enzymes activity and seeds quantity and quality were measured.
Results and Discussion:Fe-amino acid application significantly (P£0.05) enhanced root and shoot dry matter yield, total chlorophyll content, 1000 seed weight, seed yield, seed protein content, seed oil content, oleic acid, and number of seeds in each pod in comparison with Fe-EDTA treatment. The maximum chlorophyll content, seed protein and seed oil were observed in the Fe-glycine treatment (increased by 66.25%, 103.48% and 85.11%, respectively compared to that of control). Concentrations of ferritin, iron, zinc, and nitrogen in soybean seeds were also higher in Fe-amino acid chelate treatments compared to Fe-EDTA. The effect of Fe-amino acid chelates on the Fe content in seeds was in the order Fe-glycine> Fe-glutamine> Fe-lysine> Fe-histidine>  Fe-arginine. Catalase activity (EC 1.11.1.6)
and ascorbate peroxidase (EC 1.11.1.11) significantly (P£0.05) increased in all Fe-amino acid chelates treatments compared to Fe-EDTA treatment.
Conclusion: According to the results, Fe-amino acid chelates could provide the required amount of iron to soybean and this resulted in seed quality enhancement. Therefore, Fe-amino acids can be used as more efficient fertilizers than Fe-EDTA in nutrient solution, and they can be used as an alternative to Fe-EDTA to supply Fe in nutrient solutions.

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References
 
Aebi, H. (1984). Catalase in vitroMethods in Enzymology, 105‌(12), 121-126. doi.org/10.1016/ S0076-6879 (84)05016-3.
Alhasawi, A. Z. Castonguay, N. D., Appanna, C., & Appanna, V. D. (2015). Glycine metabolism and anti-oxidative defense mechanisms in Pseudomonas fluorescens. Microbiological Research, 171‌(8), 26-31. doi: 10.1016/j.micres.2014.12.001.
Amin, A. A., Gharib, F. A. E., El-Awadia, M., & Rashad, E. S. M. (2011). Physiological response of onion plants to foliar application of putrescine and glutamine. Scientia Horticulturae, 129‌(3), 353-60. doi.org/10.1016/j.scienta.2011.03.052.
Aravind, P., & Prasad, M. N. V. (2005). Cadmium-induced toxicity reversal by zinc in Ceratophyllum demersum L. (a free floating aquatic macrophyte) together with exogenous supplements of amino and organic acids. Chemosphere, 61‌(11), 1720-1733. doi: org/10.1016/j. chemosphere.2005.03.088.
Arnon, D. I. (1949). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24(1), 1-15. doi: org/10.1104/ pp.24.1.1
Basgel, S., & Erdemoglu, S. (2006). Determination of mineral and trace elements in some medicinal
herbs and their infusions consumed in Turkey. Science of the Total Environment, 359‌(3), 82-89. doi: 10.1016/j.scitotenv.2005.04.016.
Boghdady, M. S., Selim, D. A. H., Nassar, R. M. A., & Salama, A. M. (2016). Influence of foliar spray with seaweed extract on growth, yield and its quality, profile of protein pattern and anatomical structure of chickpea plant (Cicer arietinum L.). Middle East Journal of Applied Sciences, 6‌(1), 207-221.
Bradford, M. (1976). A rapid and sensitive method
for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72‌(7), 248-254. doi: 10.1016/0003-2697(76)90527-3.
Briet, J. F., Duc, C., Ravet, K., & Gaymard, F. (2010). Ferritins and iron storage in plants. Biochimica Biophysica Acta (BBA) General Subjects, 1800‌(8), 806–814. doi: 10.1016/j.bbagen.2009.12.003.
Dehnavard, S., Souri, M. K., & Mardanlu, S. (2017). Tomato growth responses to foliar application of ammonium sulfate in hydroponic culture. Journal of Plant Nutrition, 40‌(3), 315-323. doi.org/10.1080/ 01904167.2016.1240191.
Eskandari, S., Khoshgoftarmanesh, A. H., & Sharifnabi, B. (2017). The effect of foliar-applied manganese
in mineral and complex forms with amino acids
on certain defense mechanisms of cucumber (Cucumis sativus L.) against powdery mildew. Journal of Plant Growth Regulation, 37‌(2), 481-490. doi:10.1007/s00344-017-947-x.
Fahimi, F., Souri M. K., & Yaghobi, F. (2016). Growth and development of greenhouse cucumber under foliar application of Biomin and Humifolin fertilizers in comparison to their soil application and NPK. Journal of Science and Technology of Greenhouse Culture, 7‌(1), 143-152. doi: 10.18869/acadpub.ejgcst.7.1.143.
Farno, L. A. (2005). Oil and fatty acid profiles of soybeans (Maturity Groups IV, V, and VI). Eastern Kentucky University, Kentucky.
Garcia, A. L., Madrid, R., Gimeno, V., Rodriguez-Ortega, W.M., Nicolas, N., & Garcia-Sanchez, F. (2011). The effects of amino acids fertilization incorporated to the nutrient solution on mineral composition and growth in tomato seedlings. Spanish Journal of Agricultural Research, 9‌(3), 852-61. doi: 10.5424/sjar/20110903-399-10.
Ghasemi, S., Khoshgoftarmanesh, A. H., & Afyuni, M. (2014). Iron (II)–Amino acid chelates alleviate salt-stress induced oxidative damages on tomato grown in nutrient solution culture. Scientia Horticulturae,
165‌(1), 91-98. doi: 10.1016/j.scienta.2013.10.037.
Ghasemi, S., Khoshgoftarmanesh, A. H., Hadadzadeh, H., & Jafari, M. (2012). Synthesis of iron-amino acid chelates and evaluation of their efficacy as iron source and growth stimulator for tomato in nutrient solution culture. Journal of Plant Growth Regulation, 31‌(8), 498-508. doi: 10.1007/s00344-012-9259-7.
Gill, S., & Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry,
48‌(12), 909-930. doi: 10.1016/j.plaphy.2010.08.016.
Haydon, M., & Cobbett, C. S. (2007). Transporters
of ligands for essential metal ions in plants. New Phytologist, 174‌(3), 499-506. doi: 10.1111/j.1469-8137.2007.02051.x.
Jalili Sheshbahre, M., Movahhedi Dehnavi, M., & Hashemi Jazi, S. M. (2013). Quantity and quality improving of soybean yield by zinc and iron foliar application under drought stress. Plant Productions36‌(2), 111-122. [In Farsi with English abstract]
Jashani, R., Fateh, E., & Aynehband, A. (2017). Effect of Thiobacillus and Nitrocara Biological Fertilizers and Foliar Application of Zinc and Iron on Some Qualitative Characteristic and Remobilization of Rapeseed (Brassica napus L.). Plant Productions, 40‌(1),1-14. doi: 10.22055/PPD.2017.12067. [In Farsi with English abstract]
Katyal, J. C., & Sharma, B. D. (1980). A new technique of plant analysis to resolve iron chlorosisPlant and Soil, 55‌(1), 105-119. doi: 10.1007/BF02149714.
Keutgen, A., & Pawelzik, E. (2008). Contribution of amino acids to strawberry fruit quality and their relevance as stress indicators under NaCl salinity. Food Chemistry, 111‌(3), 642-647. doi:10.1016/ j.foodchem.2008.04.032.
Kocira, S., Kocira, A., Kornas, R., Szmigielski, M., & Krajewska, M. (2017). Effects of seaweed extract on yield and protein content of two common bean (Phaseolus vulgaris L.) cultivars. Legume Research, 41‌(4), 589-593. doi:10.18805/LR-383.
Kocira, S., Szparaga, A., Kocira, A., Czerwinska, E., Wojtowicz, A., & Bronowicka-Mielniczuk,
U. (2018). Modeling biometric traits, yield and nutritional and antioxidant properties of seeds
of three soybean cultivars through the application of biostimulant containing seaweed and amino acids. Frontiers in Plant Science, 9‌(2), 388. doi:10.3389/fpls.2018.00388.
Liao, X., Lv, C., Zhang, X., Masuda, T., Li, M., & Zhao, G. (2012). A novel strategy of natural plant ferritin to protect DNA from oxidative damage during iron oxidation. Free Radical Biology and Medicine, 53(2), 375-382. doi: 10.1016/j.freeradbiomed.2012.05.003.
Mengel, K. (1994). Iron availability in plant tissues-iron chlorosis on calcareous soils. Plant and Soil, 165 (2), 275-83. doi.org/10.1007/BF00008070.
Mohammadipour, N., & Souri, M. K. (2019). Effects of different levels of glycine in the nutrient solution on the growth, nutrient composition and antioxidant activity of coriander (Coriandrum sativum L.). Acta Agrobotanica, 72(1), 1759. doi: 10.5586/aa.1759.
Monica, R. C., & Cremonini, R. (2009). Nanoparticles and higher plants. Caryologia, 62(2), 161-165. doi: org/10.1080/00087114.2004.10589681.
Murphy, J., & Riley, J. P. (1962). A Modified Single Solution Method for the Dermination of Phosphate in Natural Waters. Analytica Chimica Acta, 27(8), 31-36. doi: org/10.1016/S0003-2670(00)88444-5.
Nakano, Y., & Asada, K. (1981). Hydrogen peroxide
is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 22(5), 867-880.
Noroozlo, Y. A., Souri, M. K., & Delshad, M. (2019). Stimulation effects of foliar applied glycine and glutamine amino acids on lettuce growth. Open Agriculture, 4(1), 164-172. doi.org/10.1515/opag-2019-0016.
Qiao, Z., & Murray, F. (1998). The effects of NO2 on the uptake and assimilation of nitrate by soybean plants. Environmental and Experimental Botany, 39(1), 33-40. doi: 10.1016/S0098-8472(97)00023-3.
Rajabbeigi, E., Ghanati, F., Abdolmaleki, P., & Payez, A. (2013). Antioxidant capacity of parsley cells (Petroselinum crispum L.) in relation to iron-induced ferritin levels and static magnetic field. Electromagnetic Biology and Medicine, 32(4), 430-441. doi: 10.3109/ 15368378.2012.736441.
Saez-Plaza, P., Navas, M. J., Wybraniec, S., Michałowski, T., & Asuero, A. G. (2013). An overview of the kjeldahl method of nitrogen determination. Part II. Sample preparation, working scale, instrumental finish, and quality control. Critical Reviews in Analytical Chemistry, 43(4), 224-272. doi: 10.1080/ 10408347.2012.751786.
Sanchez, A. S., Juarez, M., Sanchez-Andreu, J., Jorda, J., & Bermudez, D. (2005). Use of humic substances and amino acids to enhance iron availability for tomato plants from applications of the chelate Fe EDDHA. Journal of Plant Nutrition, 28(11), 1877-
1886. doi.org/10.1080/01904160500306359.
Soares, L. H., Dourado-Neto, D., Fagan, E. B., Teixeira, W. F., dos, M. R., & Reichardt, K. (2016). Soybean seed treatment with micronutrients, hormones and amino acids on physiological characteristics of plants. African journal of agricultural research, 11(35), 3314-3319. doi: 10.5897/AJAR2016.11229.
Souri, M. K. (2016). Aminochelate fertilizers: The new approach to the old problem; a review. Open Agriculture, 1(open-issue), 118-23. doi: 10.1515/ opag-2016-0016.
Souri, M. K., & Yarahmadi, B. (2016). Effect of amino chelates foliar application on growth and development of marigold (Calendula officinalis) plants. Iranian Journal of Plant Production Technology, 15(2), 109-119. [In Farsi with English abstract]
Souri, M. K., Naiji M., & Aslani, M. (2018). Effect of Fe-glycine aminochelate on pod quality and iron concentrations of bean (Phaseolus vulgaris L.) under lime soil conditions. Communications in Soil Science and Plant Analysis, 49(2), 215-224. doi.org/10.1080/ 00103624.2017.1421655.
Souri, M. K., Yaghoubi Sooraki, F., & Moghadamyar, M. (2017). Growth and quality of cucumber, tomato, and green bean plants under foliar and soil applications of an amino chelate fertilizer. Horticulture, Environment and Biotechnology, 58(6), 530-536. doi.org/10.1007/s13580-017-0349-0.
Sparks, D. L. (1996). Methods of soil analysis. SSSA, ASA Publishing: Madison.
Vadas, T. M., Zhang, X., Curran, A. M., & Ahner, B. A. (2007). Fate of DTPA, EDTA and EDDS in hydroponic media and effects on plant mineral nutrition, Journal of Plant Nutrition, 30(8), 1229-1246. doi.org/10.1080/01904160701555119.
Vassil, A. D., Kapulnik, Y. I., & Salt, D. E. (1998). The role of EDTA in lead transport and accumulation by Indian mustard. Plant Physiology, 117(2), 447-453. doi: 10.1104/pp.117.2.447.
Wang, H., & Jin, J. (2007). Effects of zinc deficiency and drought on plant growth andmetabolism of reactive oxygen species in maize (Zea mays L.). Agricultural Sciences in China, 6(8), 988-995. doi: org/10.1016/S1671-2927(07) 60138-2.
Weiland, M., Mancuso, S., & Baluska, F. (2015). Signaling via glutamate and GLRs in Arabidopsis thaliana. Functional Plant Biology, 43(1), 1-25. doi: org/10.1071/FP15109
Zewail, R. M. Y. (2014). Effect of seaweed extract and amino acids on growth and productivity and some bioconstituents of common bean (Phaseolus vulgaris L.) plants. Journal of Plant Production, 5(8), 1441-1453. doi: 10.21608/jpp.2014.64669.