Effect of Iron-Amino Acid Chelates on Antioxidant Capacity and Nutritional Value of Soybean

نوع مقاله: انگلیسی

نویسنده

هیات علمی گروه خاکشناسی، دانشکده کشاورزی، دانشگاه لرستان، لرستان، ایران

10.22055/ppd.2020.30728.1810

چکیده

There are various Fe fertilizers to eliminate iron deficiency in crops. There are reported some disadvantages for iron-chelate fertilizers as one of the commonly used source of iron supply to plants. The objective of this paper was to evaluate the effect of some various Fe- chelates, (Fe-arginine, Fe-glycine, Fe-histidine, Fe-lysine, Fe-glutamine and Fe-EDTA) on the physiological properties and nutraceutical potential of soybean (Glycine max (L.) Merr.) grown in nutrient solution. The experiments had a randomized complete block design with three replicates in greenhouse of Faculty of Agriculture at Lorestan University. Fe-amino acid application significantly enhanced root and shoot dry matter yield, total chlorophyll content, 1000 seed weight, seed protein content, seed oil content, oleic acid, and number of seeds in each pod in comparison with Fe-EDTA. The maximum chlorophyll content, seed protein and seed oil was observed in Fe-glycine treatment (increased by 66.25%, 103.48% and 85.11%, respectively compared to that of control). Concentrations of ferritin, iron, zinc, and nitrogen in soybean seeds were also higher in Fe-amino acid chelate treatments compared with Fe-EDTA. Catalase activity (EC 1.11.1.6) and ascorbate peroxidase (EC 1.11.1.11) significantly increased in all Fe-amino acid chelates treatments compared to Fe-EDTA. According to the results, Fe-amino acid chelates could provide the required amount of iron to soybean and this resulted in seed quality enhancement. Therefore, Fe-amino acids can be used as more efficient fertilizers than Fe-EDTA in nutrient solution.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of Iron-Amino Acid Chelates on Antioxidant Capacity and Nutritional Value of Soybean

نویسنده [English]

  • Mahboobeh Jalali
Department of Soil Sciences, College of Agriculture, Lorestan University, Lorestan, Iran
چکیده [English]

Background and Objectives
Supplying a sufficient amount of available iron (Fe) for plant growth in hydroponic nutrient solutions is a great challenge. There are various Fe fertilizers to eliminate iron deficiency in crops. The chelators commonly used to supply Fe in nutrient solutions have several disadvantages and may negatively affect plant growth. The objective of this paper was to evaluate the effect of some various Fe- chelates, (Fe-arginine, Fe-glycine, Fe-histidine, Fe-lysine, Fe-glutamine and Fe-EDTA) on the physiological properties and nutraceutical potential of soybean (Glycine max (L.) Merr.) grown in nutrient solution.

Materials and Methods
The experiments had a randomized complete block design with three replications and the treatments were arranges in factorial combination. The plants were grown in a greenhouse of Faculty of Agriculture at Lorestan University in 2018. In this study Fe- chelates were synthesized. Then, soybean seeds were sterilized and germinated in 11 μm filter paper. Seven-day seedlings were transplanted into perlite and watered regularly with distilled water for a week. Then, Two-pair leaflet seedlings were placed in continuously aerated solution under controlled conditions of greenhouse. Six different iron treatments (at Fe level=50 µM) were applied including: Fe-EDTA, Fe-arginine, Fe-Glycine, Fe-histidine, Fe-glutamine, and Fe-lysine. The plant leaves collected at 10-leaves stage. The seed collection was done in maturity of harvest (R8). Afterwards, physiological parameters, antioxidant enzymes activity and seeds quantity and quality were measured.
Results
Fe-amino acid application significantly (P 0.05) enhanced root and shoot dry matter yield, total chlorophyll content, 1000 seed weight, seed yield, seed protein content, seed oil content, oleic acid, and number of seeds in each pod in comparison with Fe-EDTA treatment. The maximum chlorophyll content, seed protein and seed oil was observed in Fe-glycine treatment (increased by 66.25%, 103.48% and 85.11%, respectively compared to that of control). Concentrations of ferritin, iron, zinc, and nitrogen in soybean seeds were also higher in Fe-amino acid chelate treatments compared with Fe-EDTA. The effect of Fe-amino acid chelates on Fe content in seeds was in the order Fe-glycine> Fe-glutamine> Fe-lysine> Fe-histidine> Fe-arginine. Catalase activity (EC 1.11.1.6) and ascorbate peroxidase (EC 1.11.1.11) significantly (P 0.05) increased in all Fe-amino acid chelates treatments compared to Fe-EDTA treatment.

Discussion
According to the results, Fe-amino acid chelates could provide the required amount of iron to soybean and this resulted in seed quality enhancement. Therefore, Fe-amino acids can be used as more efficient fertilizers than Fe-EDTA in nutrient solution and they can be used as an alternative for Fe-EDTA to supply Fe in nutrient solutions.

کلیدواژه‌ها [English]

  • Antioxidant capacity
  • Amino acids
  • Biometric traits
  • Chelating agents
  • Fe-EDTA