Bioactivity of Methanolic Leaves and Stem Extracts of Adiantum capillus-veneris L. From Southeast of Marivan

نوع مقاله: انگلیسی

نویسندگان

1 Dept. of Phytochemistry, Faculty of Chemistry, University of Kashan, Kashan, Iran

2 میکروبیولوژی، گروه بیوتکنولوژی، دانشکده شیمی، دانشگاه کاشان، کاشان، ایران

10.22055/ppd.2020.29309.1757

چکیده

Background and Objectives: Adiantum capillus-veneris L. as a medicinal plant utilized to the treatment of various infectious diseases.
Materials and Methods: The antioxidant activity of the samples was determined using inhibition of free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). The extracts of Adiantum capillus-veneris L. investigated for antimicrobial activity by disc diffusion method. The extracts were tested for phytochemical. The brine shrimp lethality bioassay was applied for the cytotoxic agents.
Results: The extracts indicated the highest antioxidative activities in DPPH test (IC50 values= 56.23 ± 0.85 and 44.66 ± 1.22 μg/ml respectively, which was higher than of synthetic antioxidant butylated hydroxyl toluene (BHT) with IC50= 19 ± 1.03 μg/ml. The total phenolic contents of leaves and stem extracts, as Gallic acid equivalents, were 83.62 ± 1.87 and 147.39 ± 2.35 mg/g extract respectively. The amounts of total flavonoids compounds of leaves and stem extracts were also determined 58.50 ± 0.78 and 35.63 ± 0.39 mg respectively. Alkaloids, triterpenes, flavonoids, saponins, tannins were identified in all extracts. The leaves and stem extracts of A. capillus-veneris L. showed significant cytotoxic activity with LC50 values of 125.893 and 97.7237 (μg/ml), respectively. Vincristine sulfate (LC50 0.751 μg/ml) was used as reference standard. The water and methanolic extracts showed highest antimicrobial activity.
Conclusion: These studies suggest that the extracts of the A. capillus-veneris L. may be a promising source for novel anticancer agents. The extracts showed moderate to good antimicrobial activities.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Bioactivity of Methanolic Leaves and Stem Extracts of Adiantum capillus-veneris L. From Southeast of Marivan

نویسندگان [English]

  • MORTEZA Yazdani 1
  • fereshteh jookar kashi 2
1 Dept. of Phytochemistry, Faculty of Chemistry, University of Kashan, Kashan, Iran
2 Department of Biotechnology, Faculty of Chemistry, university of Kashan, Kashan,
چکیده [English]

Abstract:
Background and Objectives: Adiantum capillus-veneris Linn is a medicinal plant belong to Polypodiales order. Pare-siavashan is name of Adiantum capillus-veneris Linn in pharmaceutical textbooks of Iranian Traditional Medicine. A. capillus-veneris L as a medicinal plant utilized to treat various infectious diseases. In this study, the extract of A. capillus-veneris L evaluated for the first time from Kurdistan province.
Materials and Methods: The plant collected from southeast of Marivan in October 2017 from southeast of Marivan. The aim of this study was to investigate cytotoxicity, antimicrobial and antioxidant properties and screening of phytochemicals of methanol extract polar and nonpolar subfractions from the leaves and stem of A. capillus-veneris L. separately. The antioxidant activity, total phenolic content (TPC) and total flavonoid content (TFC) of the samples were determined using inhibition of free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH), Folin-Ciocalteu method, and aluminum chloride colorimetric method, respectively. Gentamicin, rifampin and nystatin were used as positive controls in antimicrobial method. Both 50% lethal concentrations (LC50) and 50% inhibitory concentration (LC50) of the samples were identified using regression analysis.
Results: The leaves and stem extracts indicated the highest antioxidative activities in DPPH test that IC50 values =56.23 ± 0.85 and 44.66 ± 1.22 μg/ml respectively, which was higher than the synthetic antioxidant butylated hydroxyl toluene (BHT) with IC50= 19 ± 1.03 μg/ml. The total phenolic contents of leaves and stem extracts, as Gallic acid equivalents, were 83.62 ± 1.87 and 147.39 ± 2.35 mg/g extract respectively. The amounts of total flavonoids compounds of leaves and stem extracts were also determined 58.50 ± 0.78 and 35.63 ± 0.39 mg respectively. Alkaloids, triterpenes, flavonoids, saponins, tannins were identified in all extracts and glycosides were detected only in water and hexane fraction extract of stem, also hexane extraction fraction of stem was devoid triterpenes and the stem and leaves which contain no tannins. The leaves water extract of A. capillus veneris L. showed maximum zone of inhibition against bactrial strains. The hexane extracts of leaves and stem of A. capillus-veneris L showed weakest antimicrobial activity. The brine shrimp lethality bioassay was applied for the isolation of anitumour and cytotoxic agents. The leaves and stem extracts of A. capillus-veneris L showed significant cytotoxic activity with LC50 values of 125.893 and 97.7237 (μg/ml), respectively. Vincristine sulfate (LC50 0.751 μg/ml) was used as reference standard of brine shrimp lethality bioassay.
Conclusion: This study suggested that the extracts of the A. capillus-veneris L. may be a promising source for novel anticancer agents. The extracts showed moderate to good antibacterial activities.

کلیدواژه‌ها [English]

  • Adiantum capillus-veneris L
  • antimicrobial activity
  • antioxidant activity
  • Phytochemical screening
  • Cytotoxicity