Document Type : English Articles

Authors

1 Associate Professor, Department of Agronomy and Plant Breeding, College of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Former Student of Plant Breeding, Department of Agronomy and Plant Breeding, College of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Research Associate Agricultural, Medical, and Industrial Research Institute, Atomic Energy Organization of Iran, Karaj, Iran

Abstract

ABSTRACT
Introduction:Sufficient genetic variation is an essential source for having a successful breeding program. Mutation is known to be a high throughput technique to induce genetic variation in plants. Irradiation is the most common method of inducing mutations in plants mutation breeding programs leading to the production of mutants with superior genotypes. Faba bean is a crop whose diversity needs to be improved for breeding programs in Iran. Unfortunately, few researches have been carried out on mutagenesis of faba bean.
Materials and Methods:In the present study, the dose response and effects of gamma irradiation have been investigated by exposing the seeds of Vicia fabacv. Saraziri to doses of 25, 35, 45, 55, 100, and 120 Gray (Gy) in the Lab at the college of Agriculture, Shahid Chamran University of Ahvaz in 2013 to 2015. The implementation of the research was under the guidance and in cooperation with the Atomic Energy Organization of Iran. The experiments were assigned as a completed block design with four replications in both laboratory and the field.
Results and Discussion:The results of the analysis of the variance indicated that there was no significant difference in germination trait of M0 generation in the seeds at various gamma irradiation doses whereas, germination rate, seedling length, and weight were significantly altered at varied doses (P≤0.05), and further responses of these traits decreased as the dosage increased. Moreover, increasing the irradiation doses caused a delay in flowering, pod setting, and pod ripening period in M2 generation. A linear regression between different characters and gamma doses was detected. Lethal dose, 50% (LD50) for fertility and seed setting, was detected as between 60-65 Gy based on reproductive traits. The principal component analysis revealed information that the first two components within the traits at the different doses were accounted for approximately 71% of the total variance. Using the biplot diagram of the first two components, 320 mutant plants appeared and the superior one within plants was identified as compared to control.
Conclusion: This research is reported the optimum dose of gamma irradiation of 50 to 55 Gy to exert mutation induction in Vicia Faba based on the LD50 of vegetative and reproductive phases. The findings of the current work acquired several promising mutants that might be used as beneficial sources to develop new faba bean cultivars.

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References
 
Ashraf, M., Cheema, A. A., Rashid, M., & Qamar, Z. (2003). Effect of gamma rays on M1 generation in basmati rice. Pakistan Journal of Botany, 35(5), 791-795.
Bhatia, C. R., Maluszynski, M., Nichterlein, K., & Vanzanten, L. (2001). Grain legume cultivars derived from induced mutation and mutation affecting nodulation. Plant Breeding and Genetics Section, 13, 2-30.
Bond, D. A., Lawes, D. A., Hawtin, G. C., Saxena, M. C., & Stephens, J. S. (1985). Faba Bean (Vicia faba L.). In R. J. Summerfield & E. H. Roberts (Eds.), Grain Legume Crops (pp. 199-265). London, Uk: William Collins Sons Co. Ltd.
Borzouei, A., Kafi, M., Khazaei, H., Naserian, B., and Majdabadi, A. (2010). Effects of gamma radiation on germination and physiological aspects of wheat (Triticum aestivum L.) seedlings. Pakistan Journal of Botany, 42(4), 2281-2290. [In Farsi with English abstract]
Cheema, A. A., & Atta, B. M. (2003). Radiosensitivity study in basmati rice. Pakistan Journal of Botany, 35(2), 197-207.
Chung, B.Y., Lee, Y. B., Beak, M. H., Kim, J. H., Wi, S. G., & Kim, S. J. (2006). Effects of low-dose gamma-irradiation on production of shikonin derivatives in callus cultures of lithospermum erythrorhizonsRadiation Physics and Chemistry, 75, 1018-1023. doi: 10.1016/j.radphyschem.2005.11.001
Dhanavel, D., Gnanamurthy, S., & Girija, M. (2012). Effect of gamma rays on induced chromosomal variation in cowpea (Vigna unguiculata L.) Walp. International Journal of Current Science, 12, 245-250.
Ellyfa, k., Ahmed, O. H., Saamin, S., & Majid, N. M. (2007). Gamma radiosensitivity study on long bean (Vigna sesquipedalis). American Journal of Applied Sciences, 4(12), 1090-1093. doi:10.3844/ ajassp.2007.1090.1093
Hallajian, M.,Muminjanov, H.,  Jamali, S. S., Vedadi, C., Naghavi, N., & Majdabadi, A. (2011). Sensitivity to gamma rays studies in two Iranian rice (Oryza sativa) genotypes. African journal of Agricultural Research, 23, 5208-5211.
Hameed, A., Shah, T. M., Atta, B. M., Haq, M. A., and Syed, H. (2008). Gamma irradiation effects on seed germination and growth, protein content, peroxides and protease activity, lipid peroxidation in Desi and Kabuli chickpea. Pakistan Journal of Botany, 40(3), 1033-1041.
Hamid, R., Siahpoosh, M. R., Mamaghani, R., & Siahpoosh, A. (2014). Evaluation the  genetic diversity of 10 Milk thistle (Silybum marianum L.) ecotypes using morphological, phenological and phytochemical traits. Plant Productions, 37(1), 37-47. [In Farsi with English abstract]
Jain, S. M. (2005). Major mutation-assisted plant breeding programs supported by FAO/IAEA. Plant Cell Tissue and Organ Culture, 82, 113-123. doi.org/10.1007/s11240-004-7095-6
Khan, K., Iqbal, M., Azim, A., Ahmad, B., Karim, F., & Sher, H. (2003). Effect of gamma irradiation on yield and yield components of barley (Hordeum vulgare L.). Pakistan Journal of Biological Sciences, 6(19), 1695-1697. doi: 10.3923/pjbs.2003.1695.1697
Maity, J. P., Mishra, D., Chakraborty, A., Saha, A., Santra, S. C., & Chanda, S. (2005). Modulation of some quantitative and qualitative characteristics in rice (Oryza sativa L.) and mung (Phaseolus mungo L.) by ionizing radiation. Radiation Physics and Chemistry, 74(5), 391–394. doi:10.1016/j.radphyschem.2004.08.005
Majeed, A., Rehman Khan, A.U., Ahmad, H., and Muhammad, Z. (2010). Gamma irradiation effects on some growth parameters of lepidium sativum L. Asian Research Publishing Network. 5(1), 1-4.
Melki, M., & Salami, D. (2008). Studies the effects of low dose of gamma rays on the behavior of Chick pea under various conditions. Pakistan Journals of Biological Science, 11(19), 2326-2330. doi: 10.3923/pjbs.2008.2326.2330
Melki, M., & Dahmani, T. H. (2009). Gamma irradiation effects on durum wheat (Triticum turgidum Desf.) under various conditions. Pakistan Journal of Biological Sciences, 12(23), 1531-1534. doi: 10.3923/pjbs.2009.1531.1534
Momeni, R., Babaeian Jelodar, N., & Bagheri, N. (2011). Effects of Gamma ray doses on  diversity of germination and agronomic traits of Rape (Brassica napus L.). Iranian Journal of Field Crops Research, 9(3), 331-339. [In Farsi with English abstract]
Omidi, M., Siahpoosh M. R., Mamghani, R., & Modarresi, M. (2015). The heat tolerance evaluating of Wheat cultivars using physiological characteristics and stress tolerance indices in Ahwaz climatic conditions. Journal of Plant Production, 38(1), 103-113. doi: 10.22055/PPD. 2015.11135 [In Farsi with English abstract]
Parsa, M., & Bagheri, A. (2006). Cereals. (Vol 1. ed.). Mashhad: Jahad daneshgahi Mashhad. [In Farsi with English abstract]
Parvan Kumar, D., Chaturvedi, A., Sreedhar, M. Aparna, M., Venu-babu, P., & Singhal, R.K. (2013). Impact of gamma radiation stress on plant height and pollen fertility in rice (oryza sativa L.). Asian Journal of Experimental Biological Sciences, 4(1), 129-133.
Rahimi, M. M., & Bahrani, A. (2011). Influence of gamma irradiation on some physiological characteristics and grain protein in Wheat (Triticum aestivum L.). World Applied Sciences Journal, 15(5), 654-659.
Saha, A., Santra, S. C., & Chanda, S. (2005). Modulation of some quantitative characteristics in rice (Orayza sativa) by ionizing radiation. Radiation Physics and Chemistry, 74, 391-394. doi: 10.1016/j.radphyschem.2004.08.005
Siddiqui, M. A., Khan, I. A., & Khatri, A. (2009). Induced quantitive variability by gamma  rays and ethylmethane sulphonate alone and in combination in rapeseed (Brassica napus  L.). Pakistan Journal ofBotany, 41(3), 1189-1195.
Singh, G., Sareen, P., & Saharan, R. (1997). Mutation studies in mung bean [vigna radiate (L.) wilczek]. Journal of Nuclear Agriculture and Biology, 26, 227-231.
Thanki, R. J., Patel, K. C., & Patel, R. D. (2007). Lipase activity of gamma-irradiated castro seeds germinated in dark. Earth Environmental Science, 10, 211-214. doi: 10.1002/jpln.19651100306
Wani, A., & Anis, M. (2008). Gamma ray and ems-induced bold-seeded high-yielding mutants in chickpea (Cicer arietinum). Turkish Journal of Biology, 32, 1-5.
Wi, S. G., Chung, B. Y., Kim, J. H., Baek, M. H.,
Yang, D. H., Lee, V., & Kim, J. S. (2005). Ultrastructural changes of cell organelles in Arabidopsis stem after gamma irradiation. Journal of Plant Biology, 48(2), 195-200. doi.org/10.1007/ BF03030408