The dose response and mutation induction by Gamma ray in Vicia faba cv. Saraziri

نوع مقاله: انگلیسی

نویسندگان

1 دانشگاه شهید چمران اهواز

2 گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز

3 دانشگاه

4 سازمان انرژی اتمی

10.22055/ppd.2020.31265.1831

چکیده

Genetic variation is essential for success in breeding programs. Mutation induction is a high throughput technique to produce genetic variation in plants. Faba bean is a crop whose diversity needs to be improved for breeding programs in Iran. Unfortunately, few researches have been carried out on mutagenesis of faba bean. In the present study the effects of gamma irradiation on faba bean (var. Saraziri) have been investigated by exposing the seeds to doses of 25, 35, 45, 55, 100, and 120 Gray in the Faculty of Agriculture of Shahid Chamran University of Ahvaz by cooperation with Atomic Energy Organization of Iran during 2013-2015. The analysis of variance results for germination traits in M0 generation indicated that there was no significant difference in germination of M0 seeds at different gamma irradiation doses, but germination rate, seedling length and weight were significantly changed at different doses (P≤0.05) and decreased by increasing dosage. A linear regression between different characters and gamma doses was detected. LD50 for fertility and seed setting was detected as between 60-65 Gy based on reproductive traits. The effects of different doses of gamma rays on agronomic traits of M2 generation including number of pods per plant, number of seeds per pod, number of seeds per plant, and seed yield per plant were significant and decreased as the irradiation dose increased; this effect on the pod sterility percentage was also significant and increased by increasing dose of irradiation. Moreover, increasing irradiation doses caused a delay in flowering, podding,

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The dose response and mutation induction by Gamma ray in Vicia faba cv. Saraziri

نویسندگان [English]

  • M. Siahpoosh 1
  • Bahar Tahmasebi 2
  • Daryoosh Nabati Ahmadi 3
  • Masoud Rahimi 4
1 .
2 scu
3 University
4 شفخئهز خقلشدهظشفهخد
چکیده [English]

Genetic variation is essential for success in breeding programs. Mutation induction is a high throughput technique to produce genetic variation in plants. Faba bean is a crop whose diversity needs to be improved for breeding programs in Iran. Unfortunately, few researches have been carried out on mutagenesis of faba bean. Genetic variation is essential for success in breeding programs. Mutation induction is a high throughput technique to produce genetic variation in plants. Faba bean is a crop whose diversity needs to be improved for breeding programs in Iran. Unfortunately, few researches have been carried out on mutagenesis of faba bean. Genetic variation is essential for success in breeding programs. Mutation induction is a high throughput technique to produce genetic variation in plants. Faba bean is a crop whose diversity needs to be improved for breeding programs in Iran. Unfortunately, few researches have been carried out on mutagenesis of faba bean. In the present study the dose response and effects of gamma irradiation on faba bean (cv. Saraziri) have been investigated by exposing the seeds to doses of 25, 35, 45, 55, 100, and 120 Gray (Gy) in the Faculty of Agriculture of Shahid Chamran University of Ahvaz by cooperation with Atomic Energy Organization of Iran during 2013-2015. The experiments were conducted in the laboratory and field in the frame of complete block design with at least 4 replications. The analysis of variance results for germination traits in M0 generation indicated that there was no significant difference in germination of M0 seeds at different gamma irradiation doses, but germination rate, seedling length and weight were significantly changed at different doses (P≤0.05) and decreased by increasing dosage. Moreover, increasing irradiation doses caused a delay in flowering, pod setting, and pod ripening period in M2 generation. A linear regression between different characters and gamma doses was detected. LD50 for fertility and seed setting was detected as between 60-65 Gy based on reproductive traits. The principal component analysis of the traits at different doses revealed that the first two components accounted for approximately 71% of the total variance. Using the biplot diagram of the first two components, 320 mutant plants were separated, and the superior mutant plants as compared with control were identified. This research reported the optimum dose of gamma irradiation (50-55 Gy) for mutation induction in V. Faba based on the LD50 of vegetative and reproductive traits and introduced several promising mutants that may produce commercial broad bean cultivars.

کلیدواژه‌ها [English]

  • LD50
  • Morphology
  • Mutagenesis
  • Phenology