Micro Propagation of Penta Rootstock (Prunus domestica) in the Two Culture Media (MS and B5)

Balapoor, Ziba; Hosseini Moghaddam, Hossein; Zarei, Mehdi; Mollashahi, Mehdi

doi:10.22055/ppd.2019.24937.1570

Document Type : Research Paper

Authors

¹ 1- M.Sc. Student of Biotechnology, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran

² Assistant Professor, Department of Plant Production, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran

³ Dept. of plant production, Faculty of Agriculture and Natural resources, Gonbad kavous university, Gonbad Kavous, Iran

https://doi.org/10.22055/ppd.2019.24937.1570

Abstract

Abstract

Background and Objectives
Culture medium and growth regulators type can influence the growth traits and for determining the most effective medium and hormone compositions an extensive studies are needed; otherwise the culture medium may have a negative effect on the growth traits. In this research the effects of two culture media (B5 and MS) supplemented with two growth regulators (IBA and BAP) with different concentrations (0.5, 0.9, and 1.5 for IBA and 0.4, 0.8, and 1.2 mg/l for BAP) alone or in combination was evaluated. Studied traits were number of leaf per shoot, shoot length, number of regenerated shoots based on completely random plan with 5 replications.

Materials and Methods
Explants provided in the beginning of spring from mother plants growing in open environment. Shoot tips and axillary buds (0.3-0.4 cm long) used as the explants. After washing with tap water, explants were immersed in ethylic alcohol for few seconds (3-4 seconds) then immersed in mercuric chloride (0.005 W/V) for 5 minutes which supplemented with few drops of tween 20 and finally washed three times with sterile distilled water. Disinfected explants cultured on medium under air laminar flow bench. Growth chamber was adjusted to 16 hours light and 25 to 27ᵒC.

Results
The results of variance analysis demonstrated significant differences between media, hormone level and interaction between hormone concentrations for traits studied. The results showed the longest shoot (6.39 cm) and the highest shoot number (13.65) obtained in MS medium supplemented with 0.8 and 0.4 mg/l BAP respectively. In this experiment the highest root number (8.41) and root length (14.75 cm) was observed in B5 and MS medium containing 0.9 and 1.5 mg/l, IBA.

Discussion
Overall the most shoot proliferation of this rootstock occurred in MS medium. New shoots in MS medium showed normal and better growth comparing to shoots obtained in B5 medium, that can be one of the results of higher amount of nitrogen (ammonium nitrite) in MS medium. In B5 medium with all hormone treatments weak growth observed for this plant. In fact this medium was made for herbaceous plants and this is one of the main reasons why penta rootstock did not showed good growth symptoms on this medium.

Keywords

Main Subjects

H: Tissue Culture

References

References

Ahmad, T., Rahman, H., Ahmad M. S. and Laghari M. H. (2003). Effect of culture media and growth regulators on micropropagation of peach rootstock GF677. Pakistan Journal of Botany, 35(3), 331-338.

Ambrozic, B., Smole, J. and Sifter, A. (1992). Micropropagation of a plum ecotype (Prunus domestica L.) as rootstock for apricot. Acta Horticulturae, 300(11), 111-114.

Andreu, P. and Marin J. A. (2004). Micropropagation enhance in vitro establishment and multiplication of new cultivars from field grown plants of Adesoto101 (Prunus insititia) rootstock. Acta Horticulturae, 658(91), 605-609.

Bolandi, A. R., Hamidi H. and Rezagholy A. A. (2016). Effects of culture media and growth regulators on propagation of rootstock GF677 in tissue culture condition. Journal of Plant Researches, 29(1), 1-14. [In Farsi]

Channuntapipat, C., Sedgley M. and Collins G. (2003). Micropropagation of almond cultivars nonpariel and neplus ultra and the hybrid rootstocks titan nemagard. Scientia Horticulturae, 98(4), 473-484.

Daneshvar Hossini, A.R., Ganji Moghadam E. and Anahid S. (2010). Effects of media cultures and plant growth regulators in micropropagation of Gisela 6 rootstock. Annals of Biological Research, 1(2), 135-141. [In Farsi]

Dobranszki, J. and Teixeira da Silva, J.A. (2010) Micropropagation of apple a review. Biotechnology Advances, 28(4), 462-488.

Erbenova, M., Paprstein F. and Sedlak J. (2001). In vitro propagation of dwarfed rootstocks for sweet cherry. Acta Horticulturae, 560(95), 477-480.

Hartmann, H. T., Kester, D. E. and Davies F. T. (1990). Plant propagation, principles and practices. New Jersey, USA: Prentice-Hall International.

Isikalan, C., Akbas, F.A., Namli, S., Tikat, E. and Basaran, D. (2008). In vitro micropropagation of almond (Amygdalus communis L.cv. Nonpareil). African Journal of Biotechnology, 7(12), 1875-1880.

Karamad, Z., Ganji Moghaddam, E. and Bolandi A .R. (2014). The effect of culture media and growth regulators on propagation of Gisela 6 rootstock. Journal of crops improvement, 16(2), 339-351.
[In Farsi]

Kornova, K. and Popov, S. (2010). Effect of growth regulators for ex vitro rooting during adaptation of in vitropropagated plants to non-sterile condition. General and Applied Plant Physiology, 36 (1-2), 69-72.

Mahdaviyan, M., Bozari, N. and Abdollahi, H. (2011). Effects of culture media and growth regulators on proliferation and rooting of a vegetative mahlab rootstock (SL-64). Seed and Plant Production Journal, 26(1), 15-26. [In Farsi]

Massai, R. and Loreti F. (2004). Preliminary observations on nine peach rootstocks grown in a replant soil. Acta Horticulturae, 658(26), 185-192.

Mohamadzadeh Moghadam, N. and Hamidi H. (2017). Investigating the effects of medium,sterilization and hormonal treatment on micropropagation of some apple (Mallus domestica Borkh.) rootstocks. Plant Productions, 40(1), 41-54. [In Farsi]

Mohammadinejad, Sh., Gholami, M., and Asnaashari, M. (2014). The effect of culture media and cytokinin on the primary steps of walnut micro propagation, selected genotype 305. Plant Productions, 37(3), 83-92. [In Farsi]

Nazary Moghaddam, R. and Yadollahi A. (2012). Micropropagation of GF677 rootstock. Journal of Agricultural Science, 4(5), 131-138. [In Farsi]

Perez-Tornero, O., Egea, J., Vanoostende, A. and Burgos L. (2000). Assessment of factors affecting adventitious shoot regeneration from in vitro cultured leaves of apricot. Plant Science, 158(1-2), 61-70.

Pinochet, J., Fernandez C., Cunill, M., Torrents J., Felipe, A., Lopez, M. M., Lastra, B. and Penyalver, R. (2002). Response of new interspecific hybrids for peach to root-knot and lesion nematodes and crown gall. Acta Horticulturae, 592(99), 707-716.

Quoirin, M. and Lepoivre, P. (1977) Improved medium for in vitro culture of Prunus sp. Acta Horticulturae, 78(54), 437-442.

Reski, R. and Abel, W. O. (1985). Induction of budding on chloronemata and caulonemata of the moss, Physcomitrella patens, using isopentenyladenine. Planta, 165(3), 354-358.

Rogalski, M., Moraes, L. K., Felisbino, C., Crestani, L., Guerra, M. P. and Silva, A. L. (2003). Acclimatization of micropropagated Prunus sp. rootstock. Revista Brasileira de Fruticultura, 25(2), 279-281.

Ruzic, D., Saric, M., Cerovic, R. and Culafic, L. (2000). Relationship between the concentration of macro elements, uptake and multiplication of cherry rootstock Gisela 5 in vitro. Plant Cell Tissue and Organ Culture, 63(1), 9-14.

Ruzic, D., Saric, M., Cerovic, R. and Culafic, L. (2003). Contents of macroelements and growth of sweet cherry rootstocks in vitro. Biologia. Plantarum, 47, 463-465.

Tatari Vernosefaderani, M., Mosavi, C. A. and Bozari, N. (2012). Micropropagation of some clonal rootstocks of stone fruits. Seed and Plant Improvement Journal, 28(1), 53-66. [In Farsi]

© 2019 by the authors. Licensee SCU, Ahvaz, Iran. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-Non Commercial 4.0 International (CC BY-NC 4.0 license) (http://creativecommons.org/licenses/by-nc/4.0/)

Micro Propagation of Penta Rootstock (Prunus domestica) in the Two Culture Media (MS and B5)

References

References

Volume 42, Issue 4December 2019Pages 441-454

Volume 42, Issue 4
December 2019
Pages 441-454