نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد گیاهان دارویی، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

2 دانشیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

3 دانشجوی دکتری فیزیولوژی تولید و پس از برداشت گیاهان باغی، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

4 دانشجوی کارشناسی ارشد میوه‌کاری ، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

به‏منظور مطالعه اثر مقادیر مختلف خاک معدنی آزومیت بر کاهش خسارات ناشی از تنش شوری در گیاه دارویی جعفری مکزیکی (Tagets minuta L.)، آزمایشی به‏صورت فاکتوریل بر پایه طرح کاملا تصادفی با 3 تکرار در گلخانه تحقیقاتی دانشگاه فردوسی مشهد انجام شد. تیمارهای آزمایش شامل 3 سطح تنش شوری آب آبیاری (صفر، 30 و 60 میلی‏مولار کلرید سدیم) و 4 سطح آزومیت (صفر، 4، 8 و 12 گرم در کیلوگرم خاک) بود. در پایان آزمایش و در مرحله گلدهی صفات بیوماس خشک اندام هوایی، محتوای نسبی آب برگ (RWC)، نشت الکترولیت، رنگیزه‏های فتوسنتزی، فعالیت آنتی‏اکسیدانی، فنل کل، کربوهیدرات محلول و محتوای پرولین اندازه‏گیری شدند. بیشترین بیوماس خشک اندام هوایی (25/30 گرم در بوته)، RWC (2/74 درصد)، کلروفیل a (mg g-1FW 5/7)، b (mg g-1FW 26/9)، کارتنوئید (mg g-1FW 73/16)، کلروفیل کل (mg g-1FW 39/33) و میزان اسانس (46/0 درصد) در تیمار بدون شوری و کاربرد آزومیت به میزان 12 گرم در کیلوگرم خاک مشاهده شد. در بالاترین سطح شوری و عدم کاربرد آزومیت نشت الکترولیت (55/83%)، فعالیت آنتی‏اکسیدانتی (27/97%)، فنل کل (mg g-1FW 10/95)، کربوهیدارت محلول (mg g-1FW 4/114) و محتوی پرولین (µM/gDW 12/0) به بیشترین میزان خود رسیدند و کاربرد آزومیت سبب کاهش این صفات شد. نتایج این تحقیق نشان داد که بیشترین خسارت وارده به گیاه در بالاترین سطح شوری (60 میلی‏مولار کلرید سدیم) مشاهده شد و کاربرد آزومیت به‏ویژه در سطح 12گرم در کیلوگرم خاک بیشترین اثر بهبوددهندگی را بر بیوماس و خصوصیات بیوشیمیایی گیاه بر جای گذاشت.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Azomite Application on Reducing the Damage of Salinity Stress in Mexican Marigold (Tagetes minuta L.)

نویسندگان [English]

  • Farahnaz Azizi 1
  • Mohammad Moghaddam 2
  • Sara Farsaraei 3
  • Din Mohammad Moshfegh 4

1 M.Sc. Student of Medicinal Plant, Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

2 Associate Professor, Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

3 PhD. Student of Production Physiology and Postharvest of Horticultural Plants, Department of Horticultural Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

4 M.Sc. Student of Phomology, Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

چکیده [English]

Background and Objectives
Salinity stress is one of the main factors that limited crop production. Salt stress causes physiological effects by decreasing soil water potential. Azomite is an inorganic mineral that contains more than 60 forms of nutrients (macro and microelements). This material modifies and improves the soil, and the high ionic exchange capacity of this substance has resulted in some of it being used to reduce the effects of salinity stress. Mexican Parsley (Tagetes minuta) is a medicinal plant from Asteraceae family that used in the food and cosmetic industries. The purpose of this experiment was to investigate biochemical response and dry aerial biomass of Tagetes minuta under salinity stress and Azomite application.
Materials and methods
A pot experiment was conducted at the research greenhouse of Ferdowsi University of Mashhad as factorial based on completely randomized design with three levels of salinity (0, 30 and 60 mM NaCl in irrigation water) and four levels of Azomite (0, 4, 8 and 12 g/kg soil) in three replications in 2018. The studied traits were included biochemical characteristics (RWC, Electrolyte leakage, chlorophyll a, chlorophyll b, carotenoid, total chlorophyll, antioxidant activity, total phenol, soluble carbohydrates, and proline) and dry biomass. Statistical analysis was performed using Minitab 17 software. The mean comparison was done by Bonferroni test at the 5% probability level. The figure drew by Microsoft Excel software.
Results and Discussion
The results of this experiment showed that the interaction effects of salinity and Azomite had a significant effect at a probability level of 1% on all of the studied traits. The highest amount of dry biomass (30.25 g.plant), RWC (74.2 %), chlorophyll a (7.5 mg.g-1FW), chlorophyll b (9.26 mg.g-1 FW), carotenoid (16.73 mg.g-1FW), total chlorophyll (33.39 mg.g-1FW) and essential oil content (0.46 %) were observed in treatment without salinity and 12 g/kg soil of Azomite. The highest amount of Electrolyte leakage (83.55 %), antioxidant activity (9727 %), Total phenol (95.10 mg.g-1FW), soluble carbohydrates (114.4 mg.g-1FW) and proline (0.12 µM/gDW) was observed on 60mM salinity and without the use of Azomite. NaCl stress can reduce nutrient uptake by the root of the plant and cause to decrease the growth of plants (Dry aerial biomass), RWC, Chlorophyll a, b, carotenoid and total chlorophyll and increase antioxidant activity, total phenol, soluble carbohydrates, and proline. Azomite in salinity stress increase water holding and inhibited the plants from Na uptake. Therefore it prevents plants from Na toxicity. The result of this study showed that the highest salinity level (60 mM NaCl) and application of Azomite especially at 12 g/kg soil level had the highest effect on ameliorating biomass and biochemical characteristics of the plant.

کلیدواژه‌ها [English]

  • Antioxidant activity
  • Dry biomass
  • Photosynthetic pigments
  • Proline
Afshar, M., & Ladan Moghadam, A. R. (2013). Evaluation the effect of salicylic acid on some quantitative, qualitative and growth on salt stress in basil (Ocimum basilicum L.) plant. Cellular and Molecular Plant Biology Journal, 10(1), 35-43. [In Farsi]
Amiri, H., & Moazeni, L. (2016). Interaction of salinity and ascorbic acid with some biochemical features in Satureja khuzestanica. Magazine of Recent Findings in Biological Sciences, 3(1), 69-79. [In Farsi]
Arshad, M., Saqib, M., Akhtar, J., & Asghar, M. (2012). Effect of calcium on the salt tolerance of different of wheat (Triticum aestivum L.) genotypes. Pakistan Journal Agriculture Science, 49(4), 497-504.
Ashraf, M., Mukhtar, N., Rehman, S., & Rha, E.S. )2004(. Salt-induced changes in photosynthetic activity and growth in a potential medicinal plant Bishop’s weed (Ammi majus L.). Photosynthetica, 42(2), 543-550.
Bahari Saravi, S. H., Pirdashti, H., & Yaghoobian, Y. (2015). Response of chlorophyll fluorescence and physiological parameters of basil plant (Ocimum basilicum L.) to the function of growth enhancing bacteria (PGPR) under salinity stress. Journal of Process and Plant Function, 6(19), 89-104. [In Farsi]
Baniasadi, F., Safari, V. R., & Maghsoodimood, A. A. (2015). The Effect of putrescine and salinity on morphological, biochemical, and pigments of (Calendula officinalis L.). Journal of Science and Technology of Greenhouse Culture, 6(1), 125-134. [In Farsi]
Barrert-Lennard, E. G. (2003). The interaction between waterlogging and salinity in higher plants: Causes, consequences and implications. Plant and Soil, 253(1), 35-54.
Bates, L. S., Waldren, R. P., & Teare, I. D. (1973). Rapid determination of free proline for water stress studies. Plant and Soil, 39(1), 205-207.
Cooper-Driver, G. A., & Bhattacharya, M. (1998). Role of phenolics in plant evolution. Phytochemistry, 49, 1165-1174.
Demiral, M. A., Aydin, M., & Yorulmaz, A. (2005). Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars. Turkish Journal of Biology, 29(2), 117-123.
Duran, R. E., Coskun, Y., & Savaskan, C. (2011). The examination of Na-Ca effect on some qualitative and quantitative characters in durum wheat plants. African Journal of Biotechnology, 10(64), 14013-14023.
Harati, E., Kashefi, B., & Matinizadeh, M. (2016). Investigation of reducing detrimental effects of salt stress on morphological and physiological traits of (Thymus daenensis Celak.) through salicylic acid application. Plant Production Technology, 16(2), 111-125. [In Farsi]
Kalhor, M., Dehestani-Ardakani, M., Shirmardi, M., & Gholam-Nejad, J. (2019). Effect of different media cultures on physico-chemical characteristics of pot Marigold (Calendula officinalis L.) plants under salt stress. Plant Productions, 42(1), 89-102. [In Farsi]
Kazemzadeh Haghighi, A. (2010). Evaluation of salinity tolerance in relation to seed germination, in nine forage sorghum varieties sorghum bicolor (L.) mohench. Journal of Plant Science Researches, 19(5), 74-81.
Khosravinejad, F., Heydari, R., & Farboodnia, T. (2009). Effect of salinity on organic solutes contents in barley. Pakistan Journal of Biological Sciences, 12, 158-162.
Lotfollahi, L., Torabi, H., & Omidi, H. (2014). Salinity effect on proline, photosynthetic pigments and leaf relative water content of chamomile (Matricaria chamomilla L.) in hydroponic condition. Journal of Plant Production Research, 22(1), 89-104. [In Farsi]
Lutts, S., Kinet, J. M., & Bouharmont, J. (1995). Changes in plant response to NaCl during development of rice (Oryza sativa L.) varieties differing in salinity resistance. Journal of Experimental Botany, 46(12), 1843-1852.
Manchanda, G., & Garg, N. (2008). Salinity and its effects on the functional biology of legumes. Acta Physiologia Plantarum, 30(6), 595-618.
Moghaddam, M., & Talebi, M. (2016). The Effects of salinity and methyl jasmonate on morphological and biochemical characteristics and photosynthetic pigments content in two basil cultivars. Journal of Planting and Seed, 32(1), 81-98. [In Farsi]
Moon, J. H., & Terao, J. (1998). Antioxidant activity of caffeic acid and dihydrocaffeic acid in lard and human low-density lipoprotein. Journal of Agricultural and Food Chemistry, 46(12), 5062-5065.
Palmer, R., & Sharon, D. (2009). 'Digging Into Soil Health'. Retrieved from http/www.growerssecret.com Today's Dietitican. Accessed 5 October 2012.
Parida, A. K., & Das, A. B. (2005). Salt tolerance and salinity effects on plants: A review. Ecotoxicology and Environmental Safety, 60(3), 324-349.
Rao, M. S. S., & Mendham, N. J. (1991). Soil–plant–water relations of oilseed rape (Brassica napus and B. campestris). The Journal of Agricultural Science, 117(02), 197-205.
Rezaei-Chiyaneh, E., Jamali, M., Pirzad, A. R., & Tofig, S. (2015). Effect of mycorrhizal fungi on some morphophysiological characters and yield of summer savory (Satureja hortensis L.) in salt stress conditions. Process and Plant Function, 5(17), 15-29. [In Farsi]
Rostami, Gh., & Moghaddam, M. (2019). Effects of Azomite on growth and some physiological and biochemical characteristics of basil under salt stress conditions. Journal of Plant Process and Function, 8(29), 299-311. [In Farsi]
Ruiz, J. M., Belakbir, A., & Romero, L. (1997). Leaf-ma cronutrient content and yield in grafting melon plants. A model to evaluate the influence of rootstock genotype. Scientia Horticulturae, 71(3), 227-234.
Sadasivam, S., & Manickam, A. (1992). Biochemical methods for agricultural sciences. India: Wiley Eastern Limited.
SaeedAkram, M., Ashraf, M., & AishaAkram, N. (2009). Effectiveness of potassium sulphate in mitigating salt induced adverse effects on different physio-biochemical attributes in sunflower (Helianthus annuus L.). Flora-Morphology, Distribution, Functional Ecology of Plants, 204(6), 471-483.
Sánchez, F. J., Manzanares, M., de Andres, E.F., Tenorio, J. L., & Ayerbe, L. (1998). Turgor maintenance, osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stress. Field Crops Research, 59(3), 225-235.
Schutz, H., & Fangmier, E. (2001). Growth and yield responses of spring wheat (Triticum aestivum L. cv. Minaret) to elevated CO2 and water limitation, Environmental Pollutions, 114(2), 187-194.
Shaki, F., Ebrahimzadeh, H., & Niknam, V. (2018). The effect of interaction between salicylic acid and penconazole on physiological and biochemical responses of safflower (Carthamus tinctorius L.) under salinity. Journal of Plant Researchers (Iranian Journal of Biology), 31(2), 469-481. [In Farsi]
Singh, V., Singh, B., & Kaul, V. K. (2003). Domestication of wild marigold (Tagetes minuta) as a potential economic crop in western Himalaya and north Indian plants. Economic Botany, 57(4), 535-544.
Singliton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3), 144-158.
Siringam, K., Juntawong, N., Cha-um, S., & Kirdmanee, C. (2011). Salt stress induced ion accumulation, ion homeostasis, membrane injury and sugar contents in salt-sensitive rice (Oryza sativa L. spp. indica) roots under isosmotic conditions. African Journal of Biotechnology, 10(8), 1340-1346.
Tabatabaie, J., & Nazari, J. 2007. Influence of nutrient concentrations and NaCl salinity on the growth, phothsyntesis and essential oil content of peppermint and lemon verbena. Turkish Journal of Agriculture, 31(4), 245-253.
Wellburn, A. R. (1994). The spectral determination of chlorophylls and b as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Journal of Plant Physiology, 144(3), 307-313.
Yamada, M., Morishita, H., Urano, K., Shiozaki, N., Yamaguchi-Shinozaki, K., Shinozaki, K., & Yoshiba, Y. (2005). Effects of free proline accumulation in petunias under drought stress. Journal of Experimental Botany, 56(417), 1975-1981.
Yamada, M., Morishita, H., Urano, K., Shiozaki, N., Yamaguchi-Shinozaki, K., Shinozaki, K., & Yoshiba, Y. (2005). Effects of free proline accumulation in petunias under drought stress. Journal of Experimental Botany, 56(417), 1975-1981.
Yarrow, D. (2000). Mineral restoration & utah rock dust: ACRES Magazine. A Voice for Ecology-Agriculture, 30(4), 14-17.
Yasar, F., Kusvuran, S., & Ellialtıoglu, S. (2006). Determination of anti-oxidant activities in some melon (Cucumis melo L.) varieties and cultivars under salt stress. Journal of Horticultural Science and Biotechnology, 81(4), 627-630.
Zhang, X., Ervin, E. H., Evanylo, G. K., & Haering, K. C. (2009). Impact of biosolids on hormone metabolism in drought- stressed tall fescue Crop. Food and Agriculture Organization of the United Nation, 49(5), 1893-1901.
 
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