نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد باغبانی، گروه علوم باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه اردکان، یزد، ایران

2 دانشیار، گروه علوم باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه اردکان، یزد، ایران

3 استادیار، مرکز ملی تحقیقات شوری، سازمان تحقیقات، آموزش و ترویج کشاورزی، یزد، ایران

4 استادیار، گروه علوم باغبانی دانشکده کشاورزی و منابع طبیعی، دانشگاه اردکان، یزد، ایران

چکیده

چکیده
انار (Punica granatum L.) یک گونه گیاهی میوه‌ای تجاری مهم از خانواده Punicaceae، می‌باشد. شوری آب آبیاری یکی از مشکلات محدودکننده کشت‌‌و‌کار آن است. هدف از انجام این پژوهش، شناسایی و معرفی متحمل‌ترین رقم تجاری انار به شوری آب‌آبیاری بود. طی یک آزمایش گلدانی در یک دوره نه ماهه ارزیابی و مقایسه تحمل به شوری سه رقم انار تجاری در سال‌های 97-1396 در سایت مرکز ملی تحقیقات شوری صورت‌گرفت. آزمایش به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با دو فاکتور 1- شوری آب آبیاری در پنج سطح 1، 3، 5، 7 و 9 دسی‌زیمنس‌بر متر و 2- رقم انار در سه سطح ("شیشه‌کپ فردوس"، "ملس‌ساوه" و "ملس‌یزدی") و با چهار تکرار و در مجموع 60 گلدان انجام شد. نتایج نشان داد که رقم و سطح شوری اثر معنی‌داری بر صفات مورفولوژیک، فیزیولوژیک و غلظت عناصر غذایی داشتند. در تمامی ارقام مورد مطالعه با افزایش سطح شوری، شاخص‌های رشدی، محتوی نسبی‌آب‌برگ و میزان کلروفیل کاهش و درصد برگ‌های نکروزه و ریزش ‌یافته افزایش یافت. بیشترین ارتفاع، تعداد انشعابات نهایی، قطر شاخه و کلروفیل کل در شوری 3 دسی‌زیمنس‌برمتر و در رقم "ملس‌یزدی" مشاهده شد. همچنین با افزایش سطح شوری میزان سدیم، کلر و نسبت سدیم به پتاسیم به‌طور معنی‌داری افزایش یافت. در بالاترین سطح شوری (9 دسی‌زیمنس‌برمتر) بیشترین میزان شاخص‌های رشدی، وزن‌تر و خشک برگ، برگ‌سبز، محتوای‌نسبی آب، کلروفیل و پتاسیم و کمترین میزان برگ نکروزه، غلظت سدیم، کلر و نسبت سدیم به پتاسیم در رقم "ملس‌یزدی" حاصل شد. به‌طور کلی در میان ارقام مورد بررسی "ملس‌یزدی" و "ملس‌ساوه" به‌ترتیب متحمل‌ترین و کم تحمل‌ترین رقم به شوری بودند. 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Comparison of Salinity Tolerant of Three Cultivars of Commercial Pomegranate (Punica granatum L.)

نویسندگان [English]

  • Zahra Jamaati 1
  • Maryam Dehestani-Ardakani 2
  • Ali Momenpour 3
  • Mostafa Shirmardi 4

1 M.Sc. Student of Horticultural Science, Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, Yazd, Iran

2 Associate Professor, Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, Yazd, Iran

3 Assistant Professor, National Salinity Research Center, Agricultural Research, Education and Extension Organization (AREEO), Yazd, Iran

4 Assistant Professor, Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, Yazd, Iran

چکیده [English]

Abstract
Background and Objectives
Pomegranate (Punica granatum L.) is an economically important commercial fruit plant species belonging to the family of Punicaceae. Saline irrigation water is currently one of the most severe limiting factors in the cultivation area. In recent years, nearly 7% of the global cultivated lands are affected by salinity, and soil salinization aggravating has been a more significant threat to the healthy and sustainable development of agriculture worldwide. Therefore, the study aimed to determine the effects of salt stress on physiological and biochemical characteristics in pomegranate cuttings to better understand the salt resistance of pomegranate and offer a reference for pomegranate cultivation on saline lands.
 
Materials and Methods
A pot experiment was conducted for nine months to evaluate and compare three Iranian cultivar salinity tolerance of commercial pomegranate in 2017-2018. The experiment was arranged in factorial based on a completely randomized design with two factors included water salinity in 5 levels of 1, 3, 5, 7, and 9 dSm-1 and three commercial pomegranate genotypes (‘Shishehcap Ferdos,’ ‘Malas Saveh’ and ‘Malas Yazdi’) in 4 replications and generally with 60 pots. At the end of the experiment, the vegetative yield and fresh and dry weight of leaves, ion leakage, relative water content, chlorophyll a, b and total were also measured. In addition, leaves were analyzed for Na+, K+, Cl–, and Na/K ratio elements.
 
Results
Results showed that cultivar and salinity levels were affected by morphological and physiological characteristics, and concentration of nutritional elements. In all the three studied cultivars, an increase in the salinity levels led to a significant decrease in the growth characteristics, the relative water content of leaves, and chlorophyll. Moreover, the percentage of leaf necrosis, fall, and ion leakage increased considerably. Also, with increasing salinity levels, Na+, Cl- and Na+/Cl- significantly increased. In the highest level of salinity (9 dS.m-1), the maximum growth characteristics, fresh and dry weight of leaves, green leaves, the relative water content of leaves, chlorophyll, and potassium, and the lowest percentage of leaf necrosis, ion leakage, Na+, Cl- and Na+/Cl- were obtained in a cultivar of ‘Malas Yazdi.’
 
Discussion
Generally, among the investigated cultivars, ‘Malas Yazdi’ and ‘Malas Saveh’ were the highest and the lowest tolerants, respectively. Our study showed that the adverse effects on physiological and morphological indexes aggravated over stress time. We inferred that it was one of the mechanisms for pomegranate alleviating the detrimental effects of salt stress. This study would provide a theoretical basis for the cultivation and utilization of pomegranate plants on saline soil.
 

کلیدواژه‌ها [English]

  • Chlorine
  • Growth characteristics
  • Malas Yazdi
  • Sodium
References
Arnon, D. I. (1949). Copper enzymes in isolated chloroplast polyphenoloxidase in Beta vulgaris. Plant Physiology, 24(1), 1-15.
Ashraf, M., & Mcneilly, T. (2004). Salinity Tolerance in Brassica Oilseeds. Critical Reviews in Plant Sciences, 23(2), 157-174.
Ates, E., & Tekeli, A. S. (2007). Salinity tolerance of Persian clover (Trifolium resupinatum var. Majus Boiss.) lines at germination and seedling stage. World Journal of Agricultural Sciences, 3(1), 71-79.
Bejaoui, F., Salas, J. J., Nouairi, I., Smaoui, A., Abdelly, C., Martínez-Force, E., & Youssef, N.B. (2016). Changes in chloroplast lipid contents and chloroplast ultrastructure in Sulla carnosa and Sulla coronaria leaves under salt stress. Journal of Plant Physiology, 198(1), 32-38.
Betran, F.J., Beck, D., Banziger, M., & Edmeades, G.O. (2003). Secondary traits in parental inbreeds and hybrids under stress and nonstress environments in tropical maize. Field Crops Research, 83(1), 51-65.
Bhantana, P., & Lazarovitch, N. (2010). Evapotranspiration, crop coefficient and growth of two young pomegranate (Punica granatum L.) varieties under salt stress. Agricultural Water Management, 97(5), 715-722.
Cao, S. Y., & Hou, L. F. (2013). Chinese fruit trees -pomegranate. China Forestry Publishing House, Beijing. Zhengzhou, China: The forestrypress.
Chartzoulakis, K. (2005). Salinity and olive: growth, salt tolerance, photosynthesis and yield. Agric. Water Manage, 78(1-2), 108-121.
Emam, Y, Hosseini, E., Rafiei, N., & Pirasteh, H. (2013). Response of early growth and sodium and potassium ions concentrations in ten barley (Hordeum vulgare L.) cultivars in salinity tension conditions. Crop Physiology Journal, 5(19), 5-15. [In Farsi]
Emami, A. (1996). Methods of plant analysis. Journal of Agricultural Research, Education & Extension Organization, 1(982), 28-58. [In Farsi]
Fernández, J. (2014). Understanding olive adaptation to abiotic stresses as a tool to increase crop performance. Environmental and Experimental Botany, 103(1), 158-179.
Hasanpour, Z., Karimi, H. R., & Mirdehghan, S. H. (2015). Effects of salinity and water stress on echophysiological parameters and micronutrients concentration of pomegranate (Punica granatum L.). Journal of plant Nutrition, 38(5), 795-807.
Horneck, D., Ellsworth, J., Hopkins, B., Sullivan, D., & Stevens, R. (2007). Managing saltaffected soils for crop production. Pacific northwest extension publication, http://extension.oregonstate.
edu/catalog/pdf/pnw/pnw601-e.pdf.
Kalhor, M., Dehestani-Ardakani, M., Shirmardi, M., & Gholam nezhad, J. (2019). Effect of different media cultures on physico-chemical characteristics of pot marigold (Calendula officinalis L.) plants under salt stress. Plant Productions, 42(1), 89-102. [In Farsi]
Karimi, H., & Hasanpour. Z. (2014). Effects of salinity and water stress on growth and macro nutrients concentration of pomegranate (Punica granatumL.). Journal of Plant Nutrition, 37(12), 1937-1951.
Khayyat, M., Tehranifar, A., Davarynejad, G. H., & Sayyari-Zahan, M. H. (2014). Vegetative growth, compatible solute accumulation, ion partitioning and chlorophyll fluorescence of ‘Malas-e-Saveh’ and ‘Shishe-Kab’ pomegranates in response to salinity stress. Photosynthetica, 52(2), 301-312.
Khoshgoftarmanesh A. H., & Naeini, M. R. (2008). Salinity effect on concentration, uptake, and relative translocation of mineral nutrients in four olive cultivars. Journal of Plant Nutrition, 31(7), 1243-1256.
Khoshgoftarmanesh, A. H. (2006) Partitioning of chlorine, sodium, and potassium and shoot growth of three pomegranate cultivars under different levels of salinity. Journal of Plant Nutrition, 29(10), 1835-1843.
Liu, C., Ming, Y., Xianbin, H., & Zhaohe, Y. (2018). Effects of salt stress on growth and physiological characteristics of pomegranate (Punica granatum L.) cuttings. Pakistan Journal of Botany, 50(2), 457-464.
Marcinska, I., Czyczyło-Mysza, I., Skrzypek, E., Filek, M., Grzesiak, S., Grzesiak, M. T., Janowiak, F., Hura, T., Dziurka, M., Dziurka, K., Nowakowska, A., & Quarrie, S. A. (2013). Impact of osmotic stress on physiological and biochemical characteristics in drought-susceptible and drought-resistant wheat genotypes. Acta physiologiae plantarum, 35(2), 451-461.
Mastrogiannidou, E., Chatzissavvidis, C., Antonopoulou, C., Tsabardoukas, V., Giannakoula, A., & Therios, I. (2016). Response of pomegranate cv. wonderful plants tο salinity. Journal of Soil Sience and Plant Nutrition, 16(3), 621-636.
Melgar, J.C., Syvertsen, J. P., Martínez, V., & Garcia-Sanchez, F. (2008). Leaf gas exchange, water relations, nutrient content and growth in citrus and olive seedlings under salinity. Biology of Plant, 52(2), 385-390.
Mirfattahi, Z., Roozban, M., Karimi, S., Tavallali, V., & Aliniaeifard, S. (2018). Screening salt tolerance in pistachio seedlings by evaluating growth, oxidative damages and mineral composition. Plant Productions, 41(2), 13-28. [In Farsi]
Moller, I. M. (2001). Plant mitochondria and oxidative stress: electron transport, NADPH turnover, and metabolism of reactive oxygen species. Annual Review of Plant Physiology and Plant Molecular Biology, 52(1), 561-591.
Momenpour, A., & Imani, A. (2018). Evaluation of salinity tolerance in fourteen selected pistachio (Pistacia vera L.) cultivars. Advances in Horticultural Science, 32(2), 249-264.
Momenpour, A., Imani, A., & Rezaie, H. (2015). Evaluation of growth characteristics and nutrient concentration in four almond (Prunus dulcis) genotypes budded on GF677 rootstock under salinity stress. Iranian Journal of Horticultural Science, 46(3), 409-427. [In Farsi]
Momenpour, A., Imani, A., Bakhshi, D., & Akbarpour, E. (2018). Evaluation of salinity tolerance of
some selected almond genotypes budded on GF677 rootstock. International Journal of Fruit Science, 18(4), 410-435.
Mousavi, S.A, Tatari, M., Mehnatkesh, A., & Haghighati B. (2009). Vegetative growth response of young seedlings of five almond cultivars to water deficit. Seed and Plant Improvment Journal, 25(4), 551-567.
Munns, R. (2002). Comparative physiology of salt and water stress. Plant Cell Environment, 25(2), 239-250.
Naeini, M. R., Khoshgoftarmanesh, A. H., & Fallahi, E. (2006). Partitioning of chlorine, sodium, and potassium and shoot growth of three pomegranate cultivars under different levels of salinity. Journal of Plant Nutrition, 29(10), 1835-1843.
Naeini, M. R., Khoshgoftarmanesh, A. H., Lessani, H., & Fallahi, E. (2005). Effects of sodium chloride induced salinity on mineral nutrients and soluble sugars in three commercial cultivars of pomegranate. Journal of Plant Nutrition, 27(8), 1319-1326.
Netondo, G. W., Onyango, J. C., & Beck, E. (2004). Sorghum and salinity: I. Response of growth, water relations, and ion accumulation to NaCl salinity. Crop Science, 47(3), 797-805.
Okhovatian-Ardakani, A. R., Mehrabanian, M., Dehghani, F., & Akbarzadeh A. (2010). Salt tolerance evaluation and relative comparison in cuttings of different pomegranate cultivars. Plant Soil Environment, 56(4), 176-185.
Parvizi, H., Sepaskhah, A. R., & Ahmadi, S. H. (2016). Physiological and growth responses of pomegranate tree (Punica granatum L.) cv. Rabab) under partial root zonedrying and deficit irrigation regimes. Agricultural Water Management, 163(1), 146-158.
Sairam, R. K., & Srivastava, G. C. (2001). Water stress tolerance of wheat (Triticum aestivum L.) variations in hydrogen peroxide accumulation and antioxidant activity in tolerant and susceptible genotypes. Agronomy and Crop Science, 186(1), 63-70
Scalia, R., Oddo, E., Saiano, F., & Grisafi, F. (2009). Effect of salinity on Puccinellia distans (L.) Parl. treated with NaCl and foliarly applied glycine betaine. Plant Stress, 3(1), 49-54.
Schachtman, D., & Lio, W. (1999). Molecular pieces to the puzzle of the interaction between potassium and sodium uptake in plants. Trends Plant Science, 4(7), 281-287.
Shannon, M.C., & Grieve, C. M. (1999). Tolerance of vegetable crops to salinity. Scientia Horticulturae, 78(1-4), 5-8.
Sivstev, M. V., Ponamareva, S.V., & Kuzmetsova, E. A. (1973). Effect of salinization and herbicide on chlorophyllase activity in tomato leaves. Fiziol Rast, 20(1), 62-65.
Staples, R.C., & Toenniessen, G. H. (1984). Salinity tolerance in plants. New York: John Wiley & Sons.
Tavousi, M., Kaveh, F., Alizadeh, A., Babazadeh, H., & Tehranifar, A. (2015). Effects of drought and salinity on yield and water use efficiency in pomegranate tree. Journal of Material and Environmental Science, 6(7), 1975-1980.
Yamasaki, S., & Dillenburg, L. C. (1999). Measurements of leaf relative water content in Araucaria angustifolia. Revista Brasilian Fisiologia Vegetal, 11(2), 69-75.
Yuan, Z. H. (2016). Research progress of molecular biology on Punica granatum L. Deciduous Fruit Trees, 48(5), 1-8.
Zhu, J. K. (2003). Regulation of ion homeostasis under salt stress. Current Opinion in Plant Biology, 6(5), 441-445.