کاهش اثرات مضر تنش شوری در گوجه‌فرنگی (Solanum lycopersicum L. cv. Mobin) با استفاده از اسید آسکوربیک

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد سبزیکاری، گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران

2 استادیار، گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران

چکیده

چکیده
شوری به فرآیندهاو ساختارهایسلولی در گیاهانآسیبمی‌رساند و موجب کاهش رشد و عملکرد می‌گردد. در پژوهش حاضر کاربرد اسید آسکوربیک برون‌زا به‌عنوان یک آنتی‌اکسیدان قوی به‌منظور بهبود مقاومت به تنش شوری در گوجه‌فرنگی مورد ارزیابی قرار گرفت. آزمایش به‌صورت فاکتوریل در غالب طرح کاملاً تصادفی با سه تکرار در گلخانه پژوهشی گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان از ابتدای زمستان تا پایان بهارسال‌های 97-1396 به اجرا درآمد. پارامترهای رشدی، عملکرد، تبادلات گازی، رنگیزه‌های فتوسنتزی، قندهای محلول و پرولین گیاه گوجه‌فرنگی تحت سطوح مختلف تنش شوری ( صفر، 25، 50 و 100 میلی‌مولار کلرید سدیم) و محلول‌پاشی با غلظت‌های مختلف اسید آسکوربیک (صفر، 5/2 و 5 میلی‌مولار) ارزیابی شد. نتایج نشان داد که شوری موجب کاهش رشد، عملکرد، رنگیزه‌های فتوسنتزی و تبادلات گازی گردید. شوری همچنین باعث افزایش میزان پرولین و قندهای محلول در برگ شد. تیمار اسید آسکوربیک در هر دو شرایط تنش شوری و بدون تنش باعث افزایش شاخص‌های رشدی و عملکرد شد. در شرایط تنش، گیاهان تیمار‌شده با اسید آسکوربیک رنگیزه‌های فتوسنتزی بیشتر و نرخ فتوسنتزی بالاتری نسبت به دیگر تیمارها نشان دادند. اسید آسکوربیک سبب کاهش معنی‌دار قندهای محلول در برگ شد. همچنین افزایش میزان اسید آسکوربیک موجب کاهش بیشتر و معنی‌دار پرولین در هر سطح شوری نسبت به دیگر تیمارها گردید. درکل، اثرات نامطلوب شوری بر گوجه‌فرنگی به‌وسیله کاربرد خارجی اسید آسکوربیک کاهش می‌یابد که درنتیجه کاهش اثرات مخرب تنش شوری و افزایش تبادلات گازی و رنگیزه‌های فتوسنتزی است. با توجه به پتانسیل اسمزی کم محلول اسید آسکوربیک و غیر سمی‌بودن، می‌توان آن‌را به‌عنوان تیماری مؤثر برای افزایش تحمل به شوری در گیاهان گوجه‌فرنگی استفاده کرد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Alleviation of the Adverse Effects of Salinity Stress in the Tomato (Solanum lycopersicum L. cv. Mobin) by Application of Ascorbic Acid

نویسندگان [English]

  • Bahareh Hajivar 1
  • Mohammad reza Zare bavani 2
1 M.Sc. Graduate of Olericulture, Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
2 Assistant Professor, Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
چکیده [English]

Abstract
 
Background and Objectives
Salinity stress is one of the major environmental factor limiting plant growth and productivity. The adverse influence of salinity stress is evaluated on all plant levels. Ascorbic acid is a small, water-soluble antioxidant molecule that acts as a primary substrate in the pathway for detoxification and neutralization of superoxide radicals and singlet oxygen. Ascorbic acid has been shown to play multiple roles in plant growth, such as in cell division, cell wall expansion, and other developmental processes. The objective of the present experiment was to investigate the effects of the exogenous application of ascorbic acid on the growth parameters and photosynthesis attributes of tomato plants.
 
Materials and Methods
The experiment was conducted as a factorial in a completely random design with three replications in greenhouse conditions. Salinity stress was applied by 0, 25, 50, 100 mM NaCl in modified Hoagland solution. Ascorbic acid treatments (0, 2.5 and 5 mM) were sprayed on the leaves every three days until the end of experiment. Leaf, stem, and root fresh and dry weight, total fresh and dry weight, yield per plant, net photosynthesis, Stomata conductance, transpiration, internal CO2 concentration, chlorophyll a, b and total chlorophyll, proline and total soluble sugar content were evaluated.
 
Results
In the present experiment, salinity stress sharply reduced the leaf, stem, root and total fresh and dry weight, yield per plant, net photosynthesis, stomata conductance, transpiration, internal CO2 concentration, chlorophyll a, b and total chlorophyll content whereas the exogenous treatment of AsA appreciably delayed the loss of all studied traits. Foliar application of ascorbic acid under both stress and non-stress conditions improved the growth parameters and photosynthesis attributes. Salinity stress also increased total soluble sugar content and proline accumulation in the leaf of the tomato plant. Application of ascorbic acid under both non-stress and salinity stress conditions decreased total soluble sugar content and proline accumulation and reduced the effects of salinity stress.
 
Discussion
This experiment showed that treating the tomato (Solanum lycopersicum L. cv. Mobin) with 5 mM ascorbic acid substantially influences several metabolic processes, leading to the increased growth. The protection of the plant against salinity stress by using of ascorbic acid was possibly to be caused indirectly as a result of increasing amount of chlorophyll which plays an essential role in photosynthesis process and then formation of carbohydrates. Thus, it might be concluded that exogenously applied ascorbic acid has low external osmotic potential and ion toxicity and it might be effective in amelioration of the adverse effects of salt stress.
 
 

کلیدواژه‌ها [English]

  • Gas Exchanges
  • Growth parameters
  • Proline
  • Total Soluble Sugar
References
Ahmadi, M. and Souri, M. K. (2018). Growth and mineral elements of coriander (Corianderum sativum L.) plants under mild salinity with different salts. Acta Physiologia Plantarum, 40(11), 94-99.
Akram, N. A., Shafiq, F. and Ashraf, M. (2017). Ascorbic acid-A potential oxidant scavenger and its role in plant development and abiotic stress tolerance. Frontiers in Plant Science, 8(613), 1-17.
Alhasnawi, A. N., Kadhimi, A. A., Yusoff, W. M. W., Zain, C. R. C. M., Isahak, A. and Alhasnawi, A. N. (2015). Exogenous application of ascorbic acid ameliorates detrimental effects of salt stress in rice (MRQ74 and MR269) seedlings. Asian Journal of Crop Science, 7(3), 186-196.
Aly, A. A., Khafaga, A. F. and Omar, G. N. (2012). Improvement the adverse effect of salt stress in Egyptian clover (Trifolium alexandrinum L.) by AsA application through some biochemical and RT-PCR markers. Journal of Applied Phytotechnology in Environmental Sanitation, 1(2), 91-102.
Arafa, A. A., Khafagy, M. A. and El-Banna, M. F. (2009). The effect of glycinebetaine or ascorbic acid on grain germination and leaf structure of sorghum plants grown under salinity stress. Australian Journal of Crop Science, 3(5), 294-304.
Ashraf, M. and Harris, P. J. C. (2013). Photosynthesis under stressful environments, an overview. Photosynthetica, 51(1), 163-190.
Athar, H. R., Khan, A. and Ashraf, M. (2008). Exogenously applied ascorbic acid alleviates salt-induced oxidative stress in wheat. Environmental and Experimental Botany, 63(1-3), 224-231.
Athar, H. R., Khan, A. and Ashraf, M. (2009). Inducing salt tolerance in wheat by exogenously applied ascorbic acid through different modes. Journal of Plant Nutrition, 32(11), 1799-1817.
Azarmi, R. and Chaparzadeh, N. (2018). The effect of salinity and fruit ripening stage on some quantitative and quantitative characteristics of tomato in hydroponics. Plant Productions, 41(2), 91-102. [In Farsi]
Bandehagh, A., Toorchi, M., Mohammadi, A. and Kasemnia, H. (2008). Growth and osmotic adjustment of canola genotypes in response to salinity. Journal of Food Agriculture and Environment, 6(2), 201-208.
Bates, L. S., Waldren, R. P. and Tear, I. D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39(1), 205- 207.
Chandna, R., Azooz, M. M. and Ahmad, P. (2013). Recent advances of metabolomics to reveal plant response during salt stress. In P. Ahmad, M. M. Azooz, and M. N. V. Prasad (Eds.), Salt stress in plants: signalling, omics and adaptations (pp: 1-14), New York: Springer Science+Business Media.
Chaves, M. M., Flexas, J. and Pinheiro, C. (2009). Photosynthesis under drought and salt stress: Regulation mechanisms from whole plant to cell. Annals of Botany, 103(4), 551-560.
Chenarani, M., Safipour Afshar, A. and Saeed Nematpour, F. (2015). Physiological and biochemical responses of chickpea (Cicer arietinum L.) to ascorbic acid under salinity stress. Iranian Journal of Plan Physiology and Biochemistry, 1(1), 63-76. [In Farsi]
Cuartero, J. and Munoz, R. F. (1999). Tomato and salinity. Scientia Horticulture, 78(1-4), 83-125.
Daneshmand, F. (2013). The effect of ascorbate pre-treatment on tomato plant under drought stress: oxidative stress, osmolytes, phenolics and protein. Iranian Journal of Plant Biology, 5(18), 53-66. [In Farsi]
Dolatabadian, A., Modarres-Sanavy, S. and Ahmadian-Chashmi, N. (2008). The effects of foliar application of ascorbic acid (vitamin c) on antioxidant enzymes activities, lipid peroxidation and proline accumulation of canola (Brassica napus L.) under conditions of salt stress. Journal of Agronomy and Crop Science, 194(3), 206-213.
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28(3), 350- 356.
Ejaz, B., Sajid, Z. A. and Aftab, F. (2012). Effect of exogenous application of ascorbic acid on antioxidant enzyme activities, proline contents, and growth parameters of Saccharum spp. hybrid cv. HSF-240 under salt stress. Turkish Journal of Biology, 36(1), 630-640.
El-Tohamy, W. A., El-Abagy, H. M. and El-Greadly, N. H. M. (2008). Studies on the effect of putrescine, yeast and vitamin C on growth, yield and physiological responses of eggplant (Solanum melongena L.) under sandy soil conditions. Australian Journal of Basic and Applied Sciences, 2(2), 296-300.
Epstein, E. (1972). Mineral nutrition of plants: Principles and perspectives. New York: Wiley.
Farokhzad, A. and Asghari, M. (2016). Effect of foliar spray with ascorbic acid on some qualitative characteristics and improving color of apple fruit (Malus domestica). Plant Productions, 39(3), 113-125. [In Farsi].
Ghorbanli, M., Ahmadi, F., Monfared, A. and Bakhshi Khaniki, Gh. (2012). Effect of salt stress and its interaction with ascorbate on catalase, ascorbate peroxidase activity, proline and malondialdehyde in Cuminum cyminum L. four weeks after germination. Iranian Journal of Medicinal and Aromatic Plants, 28(1), 14-27. [In Farsi]
Hnilickova, H., Hnilicka, F., Martinkova, J. and Kraus, K. (2017). Effects of salt stress on water status, photosynthesisand chlorophyll fluorescence of rocket. Plant, Soil and Environment, 63(8), 362-367.
Kaur, H. and N. Gupta. (2018). Ameliorative Effect of Proline and Ascorbic Acid on Seed Germination and Vigour Parameters of Tomato (Solanum lycopersicum L.) Under Salt Stress. International Journal of Current Microbiology and Applied Sciences, 7(1), 3523-3532.
Khan, T. A., Mazid, M. and Mohammad, F. (2011). A review of ascorbic acid potentialities against oxidative stress induced in plants. Journal of Agrobiology, 28(2), 97-111.
Khedr, A. H. A., Abbas, M. A. and Wahid, A. A. (2003). Proline induces the expression of salt-stress-responsive proteins and may improve the adaptation of Pancratium maritimum L. to salt-stress. Journal of Experimental Botany, 54(392), 2553-2562.
Lichtenthaler, H. K. and Buschmann, C. (2001). Extraction of photosynthetic tissues: Chlorophylls and carotenoids. In Wrolstad, R. E., Acree, T. E., Decker, E. A., Penner, M. H., Reid, D. S. and Schwarts, S. J. (Eds.), Current protocols in food analytical chemistry (pp. F4.2.1–F4.2.6). New York: John Wiley and Sons.
Mimouni, H., Wasti, S., Manaa, A., Gharbi, E. and Chalh, A. (2016). Does salicylic acid (SA) improve tolerance to salt stress in plants? A study of SA effects on tomato plant growth, water dynamics, photosynthesis, and biochemical parameters. Omics A Journal of Integrative Biology, 20(3), 180-90.
Molinari, H. B. C., Marur, C. J. and Daros, E. (2007). Evaluation of the stress-inducible production of proline in transgenic sugarcane (Saccharum spp.), osmotic adjustment, chlorophyll fluorescence and oxidative stress. Physiologia Plantarum, 130(2), 218-229.
Munns, R. and Tester, M. (2008). Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59(1), 651-681.
Saeidi-Sar, S., Abbaspour, H., Afshari, H. and Yaghoobi, S. R. (2013). Effects of ascorbic acid and gibberellin GA3 on alleviation of salt stress in common bean (Phaseolus vulgaris L.) seedlings. Acta Physiologiae Plantarum, 35(1), 667-677.
Saibo, N. J. M., Lourenco, T. and Oliveira, M. M. (2009). Transcription factors and regulation of photosynthetic and related metabolism under environmental stresses. Annals of Botany, 103(4), 609-623
Shahbazi Zadeh, E., Movahhedi Dehnavi, M. and Balouchi, H. (2015). Effects of foliar application of salicylic and ascorbic acids on some physiological characteristics of soybean (cv. Williams) under salt stress. Journal of Plant Process and Function, 4(11), 13-22. [In Farsi]
Shalata, A. and Meumann, P. M. (2001). Exogenous ascorbic acid (vitamin C) increases resistance to salt stress and reduces lipid peroxidation. Journal of Experimental Botany, 52(364), 2207-2211.
Sinha, S., Bhatt, K., Pandey, K., Singh, S. and Saxena, R. (2003): Interactive metal accumulation and its toxic effects under repeated exposure in submerged plant Najas indica Cham. Bulletin of Environmental Contamination and Toxicology, 70(1), 696-704.
Smirnoff, N. (2011). Vitamin C: the metabolism and functions of ascorbic acid in plants. Advances in Botanical Research, 59(1), 107-177.
Souri, M. K. (2016). Aminochelate fertilizers: the new approach to the old problem; a review. Open Agriculture, 1(1), 118-123.
Souri, M. K. and Aslani, M. (2018). Beneficial effects of foliar application of organic chelate fertilizers on French bean production under field conditions in a calcareous soil. Advances in Horticultural Science, 32(2), 265-272.
Sudhir, P. and Murthy, S. D. S. (2004). Effects of salt stress on basic processes of photosynthesis. Photosynthetica, 42(4), 481-486.
Tanaka, A. and Makino, A. (2009). Photosynthetic research in plant science. Plant and Cell Physiology, 50(4), 681-683.
Tedone, L., Hancock, R. D., Alberino, S., Haupt, S. and Viola, R. (2004). Long-distance transport of L-ascorbic acid in potato. BMC Plant Biology, 4(16), 1-8.
Turan, M. A., Elkarim, A. H. A. and Taban, N. (2009). Effect of salt stress on growth, stomatal resistance, proline and chlorophyll concentrations on maize plant. African Journal of Agricultural Research, 4(9), 893-897.
Vinocur, B. and Altman A. (2005). Recent advances in engineering plant tolerance to aboitic stress: achievements and limitations. Current Opinion in Biotechnology, 16(2), 123-132.
Wang, R., Liu, Sh., Zhou, F., Ding, Ch. and Hua, Ch. (2014). Exogenous ascorbic acid and glutathione alleviate oxidative stress induced by salt stress in the chloroplasts of Oryza sativa L. Journal of Biosciences, 69(5-6), 226-236.
Younis, M. E., Hasaneen, M. N. A. and Kazamel, A. M. S. (2010). Exogenously applied ascorbic acid ameliorates detrimental effects of NaCl and mannitol stress in Vicia faba seedlings. Protoplasma, 239(1-4), 39-48.
Zhang, P., Senge, M. and Dai, Y. (2017). Effect of salinity stress at different growth stages on tomato growth, yield and water use efficiency. Reviews in Agricultural Science, 48(6), 624-634.
Zhang, S., Weng, J., Pan, J., Tu, T., Yao, S. and Xu, C. (2003). Study on the photogeneration of superoxide radicals in Photosystem II with EPR spin trapping techniques. Photosynthesis Research, 75(1), 41-48.
 
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