مطالعه کاریوتایپی توده‌های سیر (Allium sativum) شمال ایران

نوع مقاله : علمی - پژوهشی

نویسندگان

1 استادیار، گروه علوم باغبانی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 دانشیار، گروه علوم باغبانی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

چکیده

چکیده
ویژگی‌های کاریوتایپی توده‌های محلی سیر شمال ایران (سیر سفید گرگان، صورتی ساری و ارغوانی رشت) در سال 1397 در دانشگاه علوم کشاورزی و منابع طبیعی گرگان مورد بررسی قرار گرفت. برای انجام آزمایش مریستم انتهایی نوک ریشه با 002/0 مول 8-هیدروکسی کینولین به‌مدت چهار ساعت در دمای چهار درجه سانتی‌گراد تیمار شد و با استفاده از اورسیین دو درصد رنگ‌آمیزی گردید. سلول‌ها در مرحله متافاز میتوز عکس‌برداری و شاخص‌های کروموزومی محاسبه شدند. تعداد کروموزوم پایه هشتx=8  (2n=2x=16)و دیپلوئید مورد تأیید قرار گرفت. بزرگترین طول کروموزوم (51/169 میکرومتر) متعلق به توده گرگان و کوچک‌ترین طول کروموزوم در توده رشت (57/136 میکرومتر) ثبت گردیدند. آنالیز کاریوتایپ متافاز نشان داد که سانترومرها به‌طور عمده در وسط (متاسنتریک) یا نزدیک وسط (ساب‌متاسنتریک) کروموزوم قرار دارند. کروموزوم‌ها در جهت کاهش طول بازوی کوتاه مرتب شدند و فرمول کاریوتایپ12m+4sm  برای تمام توده‌ها به‌دست‌آمد. کروموزوم‌های 13 تا 16 برای دو توده گرگان و رشت ساب­متاسنتریک بود. کروموزوم‌های ساب‌متاسنتریک روی کروموزوم‌های شماره 11-12 و 15-16 برای توده ساری مشاهده شد. با توجه شاخص‌های عدم تقارن از نظر تیپ کروموزوم، درصد شکل کلی و قرارگیری در کلاس  2Bطبقه‌بندی استبینز، سه توده مورد بررسی از نظر تکاملی در یک سطح قرار می‌گیرند. داده‌های کاریوتایپی، منبع جامع از اطلاعات سیتوژنتیکی فراهم می‌کند که در برنامه­های اصلاحی برای درک بهتر طبقه‌بندی، تکامل و شکل‌گیری در جنس آلیوم استفاده می­شود.
 

کلیدواژه‌ها


عنوان مقاله [English]

Karyotypic Analysis of Garlic (Allium sativum) Accessions in the North of Iran

نویسندگان [English]

  • Seyyed Javad Mousavizadeh 1
  • Mostafa Khoshhal Sarmast 1
  • Kambiz Mashayekhi 2
1 Assistant Professor, Department of Horticultural Science, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2 Associate Professor, Department of Horticultural Science, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
چکیده [English]

Abstract
Background and Objectives
Allium sativum is one of the most important, mostly used economical vegetable plants in Iran. Chromosome analysis or karyotyping is a test that evaluates the number and structure of chromosomes to study evolution. The present study was carried out to explore the cytological features, including chromosome number and karyotypic characteristics in a local cultivar of Allium sativum L., north of Iran (Gorgan, Sari, and Rasht accessions).
 
Materials and Methods
The root tip cells were treated first with 0.002 mol·L−1 8-hydroxyquinoline for 4 h at 4°C and then fixed for 24 h in fixative (1:3 glacial acetic acid/absolute alcohol) at 4°C. Finally, the root tips were softened with 1 M hydrochloric acid at room temperature for 8 min and stained for 10 min with acceto orcein 2% before being squashed between a glass slide and a coverslip. Root tip cells photographed at mitosis metaphase, which had well-separated chromosomes and a clear centromere. Long arm, short arm, the ratio of long arm to short arm, and chromosome index of mitotic chromosomes were calculated from metaphase plates. Figures summarized the idiogram for the three garlic accessions, based on the eight pairs of chromosomes’ short arm order length.
 
Results
Basic chromosome number was confirmed as x = 8 (2n = 2x = 16). All the studied accessions
cytotypes were diploid. The largest chromosome length belonged to Gorgan accesion as amount 169.51 micrometers, and the smallest chromosome length was obtained in Rasht accesion as amount 136.57 micrometers. The long arm of the chromosome was recorded between 4.85 to 6 micrometers. The short arm of the chromosome was measured between 3.18 to 3.80 micrometers. The Metaphase karyotype analysis revealed that centromeres were mainly located at the middle (metacentric - m) or near the middle (submetacentric - sm) of chromosomes. The chromosomes are arranged in decreasing order for short arm length, and the karyotype formula was obtained 12m+4sm for all accessions. Chromosomes 13 to 16 were submetacentric for both Gorgan and Rasht accessions. Submetacenteric Chromosomes were observed on chromosomes number 11-12 and 15-16 for Sari accesion. Three of the studied accessions were evolutionary located in one level according to asymmetrical karyotype, the value of TF% and separating to 2B classes based on Stebbins categories.
 
Discussion
Regarding asymmetric karyotype and according to interchromosomal asymmetry (A2) as well as difference between the range of relative length (DRL), the most were found in Rasht accession (A2=0.34; DRL=6.99). A2 and DRL are two aspects of karyotype asymmetry related to shifts of the centromere position from the median to terminal or subterminal. The karyological data provide a comprehensive cytogenetic resource useful in cytotaxonomic research and assessment of their role in the evolution of Garlic. This resource can help better understand the taxonomy, evolution, and speciation in the genus Allium and identify candidate species for breeding programs.
 

کلیدواژه‌ها [English]

  • Chromosome
  • Cytogenetic
  • Diploid
  • Metacentric
  • Mitosis
References
Chehrazi, M., Naderi, R., Shahnejat, A, A., Hasani, M, E., & Zarifi, A. (2012). Evaluation of karyotype and ploidy levels in some endemic and exotic daffodils (Narcissus sp.) genotypes. Plant Productions, 35(2), 13-27. [In Farsi]
Gheyrati, L., Naghavi, P., Zeinali, H., & Moradi, S. (2018). Karyotype diversity in 5 varieties of garlic using factor analysis. Journal of Crop Breeding, 9(24), 137-143. [In Farsi]
Hesamzadeh-Hejazi S. M., & Ziaeinasab, M. (2007). Cytogenetic study on some Hedysarum species avaible in
the natural resources gene bank of Iran. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 15(2), 85-94. [In Farsi]
Huziwara, Y. (1962). Karyotype analysis in some genera of compositeae. Further studies on the chromosome of Aster. American Journal of Botany, 49(2), 116-119.
Levan, A., Fredga, K., & Sanberge, A. A. (1964). Nomenclature for centromeric position on chromosomes. Hereditas, 52(2), 201-220.
Mousavizadeh, S.J., Hassandokht, M. R., & Kashi, A. (2017). Karyotype analysis in diploid and polyploid species of Iranian wild Asparagus. International Conference & X National Horticultural Science Congress of Iran (IrHC2017), Tehran, Iran.
Mousavizadeh, S.J., Hassandokht, M.R., Kashi, A., Gil, J., Cabrera, A., & Moreno, R.(2016). Physical mapping of 5S and 45S rDNA genes and ploidy levels of Iranian Asparagus species. Scientia Horticulturae, 211(14), 269-276.
Omidi, M., Alishah, A., & Sasanfar, B. (2014). Plant cytogenetic. Tehran: Tehran university press. [In Farsi]
Osman, S. A. M., Ata, A. M., & Gad, S. N. H. (2007). Morphological, germination, bolting and cytogenetical characteristics of fourteen promising garlic genotypes. 8th African Crop Science Conference Proceedings, El-Minia, Egypt, 2005-2012.
Paszko, B. (2006). A critical review and a new proposal of karyotype asymmetry indices. Plant Systematics and Evolution, 258(1-2), 39-48.
Ramesh, A. (2015). Karyotypic analysis in three species of Allium and some Varieties. International Research Journal of Biological Sciences, 4(9), 1-9.
Romero-Zarco, C. (1986). A new method for estimating karyotype asymmetry. Taxonomy, 35(3), 526-530.
Safari, H., Hesamzadeh-Hejazi, S. M., Jalilian, N., & Ziaeinasab, M. (2008). Study of karyotypic variation on six different populations in three sophora l. species. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 16(1), 27-36. [In Farsi]
Sheidai, M., Koobaz, P., & Zehzad, B. (2003). Meiotic studies of some Avena species and populations in Iran. Journal of Sciences, Islamic Republic of Iran, 14(2), 121-131.
Sheidai, M., Zogagi-far, Sh., Khanafshar, Sh., & Zehzad, B. (2002). Karyotypic study in some Iranian species and populations of Tulipa L. (Liliaceae). Caryologia, 55(1), 81-89.
Stebbins, G. L. (1971). Chromosomal evolution in higher plants. London: Edward Arnold LTD.
Swanson, C. P., Merz, T., & Young, W. J. (1981). Cytogenetics: The chromosome in division, inheritance, and evolution. Hoboken, New Jersey: Prentice-Hall.
Verma, S. C., & Mittal, R. K. (1978). Chromosome Variation in the Common Garlic, Allium sativum L. Cytologia, 43(2), 383-396.
Wajahatullah, M. K., & Vahidy, A. A. (1990). Karyotyping and localization of nucleolar organizer regions in Garlic, Allium sativum L. Journal of Cytologia, 55(3), 501-504.
Yaghoobi, E., & Malekzadeh, S. (2013). Genetic variation of Iranian ecotypes of Garlic (Allium sp.) using karyotype analysis. Modern Genetic, 4(35), 411-422. [In Farsi]
Yuzbasioglu, D., & Unal, F. (2004). Karyotyping, C- and NOR Banding of Allium sativum L. (Liliaceae) cultivated in Turkey. Pakistan Journal of Botany, 36(2), 343-349.
Zahedi, A., Hosseini, B., & Fattahi, M. (2018). Effect of different concentration of colchicine on some morphological and phytochemical characteristics of dracocephalum kotschyi boiss. Plant Productions, 40(4), 31-40. [In Farsi]