Document Type : Research Paper

Authors

1 M.Sc. Graduate of Horticultural Science, Department of Horticultural Science, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Associate Professor, Department of Horticultural Science, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Professor, Department of Horticultural Science, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Abstract
Background and Objectives
Olive (Olea europaea) is compatible with Mediterranean and Mediterranean-like climates. Due to its high adaptability to different environmental conditions, this plant has adapted well to the varied climates in the world. It has extensively been cultivated in some regions of Iran to the extent that some cultivars do not have any identification. Therefore, a genotype/variety may be classified under several names and several varieties under a single name. Also, lack of garden information, re-naming of imported cultivars, and non-standard morphological traits for naming has led to fundamental problems in the pomology industry. Accordingly, it is necessary and inevitable to eliminate these ambiguities and accurately identify different olives’ genotypes in Iran. Hense, the first step in identifying the genotype is morphological identification. To this aim, the present research was conducted to investigate the genetic diversity and the relationship between the olive trees cultivated in some of the cities of Khuzestan through exploring morphological traits.
 
Materials and Methods
This study was conducted in Indica, Baghmalek, Izeh, and Lali in 2015-2016. Fifty genotypes were collected from the orchards of the Andika, Baghmalek, Izeh and, Lali. The morphological traits of fruit, stone, and leaf were measured according to the IOOC guidelines and the Seed and Plant Certification and Registration Institute for Research (2007) guidelines. The variance analysis of the traits was performed using SAS 9.1 software as a nested design. Cluster analysis was used for the determination of the degree of relationship between genotypes. The main components and biplot analysis were used to reduce the number of variables.
 
Results
The results showed that there was a wide range of morphological variations among genotypes. Factor and principal components analysis showed that 12 components could justify 87.7% of the variation among genotypes. The first component explained 13.42% of the total variance. In this factor, fruit length (0.63), fruit width (0.61), fruit weight (0.61), stone shape (-0.7), stone width (0.66), stone length (-0.44), and leaf length (0.5) had a high coefficient. Moreover, traits such as length and width of fruit, stone and leaf, stone and leaf shape, curvature of leaf, stone number grooves, fruit base in position A, stone weight, stone surface in position B,  stone symmetry in position A, fruit size of lenticels, stone distribution of grooves, stone symmetry in position B, were the most important traits in genotyping and grouping of the cities studied. Based on the results of cluster analysis, the genotypes were divided into six distinct groups.
 
Discussion
In general, it was found that most of the genotypes in Baghmalek city are located in a separate cluster. In contrast, several genotypes in Lali, Izeh, and Indica are situated in a single cluster, which can be due to atmospheric differences in precipitation rates in these cities. Izeh, Lali, and Indica have lower temperatures and more atmospheric precipitation than Bagmalek. This important difference can cause the genotypes of these cities to be different compared to Baghmalek city.

Keywords

References
Ahmad pour, S., & Arji, E. (2012).  Evaluation on Zard and Roghani Olive Cultivars Responses in Different Regions of Kermanshah. Plant Productions, 35(1), 103-115. [In Farsi]
Ajamgard, F., & ShafieiZargar, A. R. (2007). Collection and evaluation of olive (Olea europaea L.) germplasm of Khuzestan province. Iranian Journal of Horticultural Science And Technology, 7(4), 229-242. [In Farsi]
Al-Ruqaie, I., Al-Khalifah, N. S., & Shanavaskhan, A. E. (2016). Morphological cladistic analysis of eight popular Olive (Olea europaea L.) cultivars grown in saudi arabia using numerical taxonomic system for personal computer to detect phyletic relationship and their proximate fruit composition. Saudi Journal of Biological Sciences, 23(1), 115-121.
Angiolillo, A., Mencuccini, M., & Baldoni, L. (1999). Olive genetic diversity assessed using amplified fragment length polymorphisms. Theoretical and Applied Genetics, 98(3), 411-421.
Badenes, M. L., Martinez-Calvo, J., & Llacer, G. )2000). Analysis of a germplasm collection of loquat (Eriobotrya japonica Lindl.). Euphytica, 114(3), 187-194.
Bartolini, G., Prevost, G., Messeri, C., & Carignani, G. (1999). Olive cultivar names and synonyms and collections detected in a literature review. Acta Horticulturae, 474(1), 159-162.
Belaj, A., Leon, L., Satovic, Z., & De la Rose, R. (2011). Variability of wild olives (Olea europaea subsp. Europaea var. sylvestris) analyzed by agro morphological traits and SSR markers. Scientia Horticulturae, 129(4), 561-569.
Bencic, D., Lolic, T., & Sindrak, T. (2010). Morphological diversity of olive (Olea europaea L.) variety lastovka phenotype in the north-western part of the island of korcula. Seed Science, 26(1), 153-159.
Cantini, C., Cimato, A., & Sani, G. (1999). Morphological evaluation of olive germplasm present in Tuscany region. Euphytica, 109(3),173-181.
D’Imperio, M., Viscosi, V., Scarano, M. T., D’Andrea, M., Zullo, B. A., & Pilla, F. (2011). Integration between molecular and morphological markers for the exploitation of olive germplasm (Olea europaea). Scientia Horticulturae, 130(1), 229-240.
Fakhraei, M., Tabar, R., Sarsaiefi, M., Fattie, A., Abadozi, Gh., Hajhasani, M., Farhadi, A., Khakizad, Gh., Azizi, Z., Samadi., B., Kiani, M., Mirakhorlee, A., Foromadi, N., Mzaffari, J., & Rafezi, R. ( 2016). Genetic Diversity Mulberry Genotypes of Iran by Using Morphological. Plant Productions, 39(3), 39-50. [In Farsi]
Hosseini-Mazinani, S. M., & Samaee, M. (2004). Evaluation of olive germplasm in Iran on the basis of morphological traits: Assessment of Zard and Rowghani cultivars. Acta Horticulturae, 634(1), 145-151.
International Olive Oil Council. (2003). Trade standard applying to olive oil and olive pomace oil. Available at: http://www.internationaloliveoil.org [Accessed Dec. 5, 2003].
Macdonald, A. J, Walter, M., Trought, M., Frampton, C. M., & Burnip, G. (2000). Survey of olive leaf spot in New Zealand. New Zealand Plant Protection, 53(1), 126-132. [In Farsi]
Nezamivand Chegini, M., Samizadeh Lahiji, H., Ramezani Malakroodi, M., & Mohsenzadeh Golfazani, M. (2015). Assessment of genetic diversity among four olive cultivars using morphological markers. Journal of Applied Crop Breeding, 3(2), 201-213. [In Farsi]
Rotondi, A., Magli, M., Riccionlini, C., & Baldoni, L. (2003). Morphological and molecular analyses for the characterization of a group of Italian olive cultivars. Euphytica, 132(2), 129-137.
Seed and Plant Certification and Registration Institute. (2007) National guidelines for differentiation, uniformity and stability in olive. Olive Specialist Task Force. Tehran: Seed and Plant Registration and Certification Institute Press. http://www.spii.ir/en-US/DouranPortal/4950. [In Farsi]
Therios, I. (2009). Olives: Crop production science in horticulture. UK: CAB Publisher.
Torkzaban, B., Ataei, S., Saboora A., Azimi M., & Hosseini Mazinani, M. (2010). Study of variation of some unknown olive genotypes in collection of tarom research station in Iran, applying morphological markers. Iranian Journal of Biology, 23(4), 520-531. [In Farsi]
 
© 2021 Shahid Chamran University of Ahvaz, Ahvaz, Iran. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0 license) (http://creativecommons.org/licenses/by/4.0/).