نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد فیزیولوژی گیاهان دارویی، ادویه‌ای و عطری، واحد خوی، دانشگاه آزاد اسلامی، خوی، ایران

2 استادیار، گروه کشاورزی، واحد خوی، دانشگاه آزاد اسلامی، خوی، ایران

چکیده

چکیده
خاکشیر گیاه دارویی ارزشمندی است که مصرف دانه‌های آن در صنایع داروسازی برای تولید موسیلاژ اهمیت زیادی دارد. متانول به‌عنوان یک محرک زیستی روی گیاهان سه کربنه مانند خاکشیر می‌تواند باعث افزایش تثبیت دی‌اکسیدکربن و کاهش تنفس نوری شود که در نتیجه ماده خشک بیشتری در این گیاهان تولید خواهد شد. این آزمایش به منظور بررسی تأثیر محلول‌پاشی متانول بر تغییرات عملکرد دانه و موسیلاژ گیاه خاکشیر در مزرعه‌ای طی سال‌های 1395-1396 در شهرستان خوی در قالب طرح بلوک‌های کامل تصادفی با 5 تیمار محلول‌پاشی و 3 تکرار اجرا شد. محلول‌پاشی در طول فصل رشد طی سه نوبت با فواصل زمانی 12 روز یک بار انجام شد. تیمارهای آزمایش شامل تیمار شاهد (محلول‌پاشی با آب‌مقطر بدون کاربرد متانول) و محلول‌پاشی با چهار غلظت مختلف متانول شامل 10، 20، 30 و 40 درصد حجمی بود. نتایج نشان داد محلول‌پاشی غلظت‌های مختلف متانول تأثیر معنی‌داری بر تعداد ساقه فرعی بوته، تعداد برگ بوته، وزن خشک بوته، تعداد غلاف در بوته، تعداد دانه در غلاف، عملکرد دانه، عملکرد موسیلاژ دانه، خاکستر دانه، شاخص تورم دانه (05/0p ≤ )، ارتفاع بوته و وزن هزار دانه (05/0p ≤ ) گیاه داشت. محلول‌پاشی متانول بهعنوان یک محرک زیستی فعال در تولید گیاهان دارویی موجب بهبود عملکرد دانه و موسیلاژ دانه خاکشیر شد. بهطورکلی بیشترین مقادیر برای خصوصیات مورد ارزیابی در القای تیمار محلول‌پاشی با غلظت 40 درصد حجمی متانول حاصل شد.
 
کلیدواژه‌ها: شاخص تورم دانه، محرک زیستی، وزن خشک

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of Methanol Spraying on Seed Yield and Mucilage of Flixweed (Descurainia sophia L.)

نویسندگان [English]

  • fatemeh Azimi 1
  • fatemeh nejatzadeh 2

1 M.Sc. Student of Physiology of Medicinal, Spice and Aroma Medicines, Faculty of Agriculture, Khoy Branch, Islamic Azad University, Khoy, Iran

2 Assistant Professor, Department of Horticulture, Faculty of Agriculture, Khoy Branch, Islamic Azad University, Khoy, Iran

چکیده [English]

Abstract
 
Background and Objectives
Descurainia sophia L. is one of the most valuable medicinal plants in the pharmaceutical industry because its seeds are used for the production of mucilages. Methanol spraying, as a biological stimulant on three carbonaceous plants, can increase carbon dioxide stability and reduce respiration, resulting in more dry matter in these plants.
 
Materials and Methods
This experiment was carried out to investigate the effect of methanol spraying on seed yield and mucilage variation of D. sophia based on randomized complete block design with five treatments and three replications in a field experiment in Khoy, Iran. The treatments consisted of the control treatment (distilled water without methanol application) with 4 concentrations of methanol spraying (containing 10, 20, 30 and 40%) carried out during the growing season three times at intervals of 12 days (36, 48, 60 days after planting). After the last spraying at the time of full maturity, the plants in each plot were taken individually. The measured morphophysiological characteristics included plant height, plant leaf number, plant stem number, plant dry weight, number of pods per plant, number of seeds per pod, 1000 seed weight and seed yield. Also, the measured phytochemical traits included seed ash content, mucilage percentage and yield, and inflation index.
 
Results
Results showed that foliar application of different concentrations of methanol had a significant effect on number of plant stems, number of plant leaves, plant dry weight, number of pods per plant, number of seeds per pod, grain yield, seed mucilage yield, seed ash, seed inflation index
(p ≤ 0.01), plant height and 1000 seed weight (p ≤ 0.05). Foliar application of different concentrations of methanol had a significant effect on all morphological and functional components. To be more precise, methanol spray had a significant effect on plant height, number of stems, plant leaf number, shoot dry weight, number of pods per plant, number of seeds per pod, 1000 seed weight and seed yield, but the effect of year on none of the measured traits was meaningful. Also, the interaction between the year and methanol spraying on the traits of plant leaf number, number of seeds per pod and 1000 seed weight had a significant effect, but the remaining traits were not affected considerably. Combined analysis of variance showed that the year effect had no noticeable effect on seed ash. While methanol concentration had a significant effect (p≤ 0.01) on the seed ash content, the interaction of methanol in different years of experiment was not significant on this characteristic.
 
Discussion
Regular methanol spraying, as an active bioavailable and a new method in the production of medicinal plants, improved seed yield and mucilage of Descurainia sophia seed. Generally, the highest values for the evaluated properties were obtained in the induction of 40% volumetric methanol solution.

کلیدواژه‌ها [English]

  • Biological stimulus
  • Dry weight
  • Seed inflation index
References
Aghaabbasi, K., Dehghan, E., Baghizadeh, A. and Dashti, H. (2013). Comparing the effect of ethanol extracts of Descurainia sophia (L.) Seed and althaea officinalis root on streptococcus pyogenes. Zahedan Journal of Research in Medical Sciences, 10(15), 29-33.
Barnes, J., Anderson, L. A. and Phillipson, J. D. (2007). Herbal medicines. London: Pharmaceutical Press.
Cherazi, M., Pourghasemi, D. and Khoshbakht, M. (2018). The effect of planting methods and calcium nanoparticles spray on quality, quantity and vase life of gladiolus hybrida cv. magma. Plant Productions, 41(2), 55-67. [In Farsi]
Dadkhah, A. S. and Kafi, M. (2012). Effect of salt stress on germination and seedling growth of four medicinal plants. Iranian Journal of Applied Research, 10(1), 25-32.
Downie, A., Miyazaki, A., Bohnert, H., John, P., Coleman, J., Parry, M. and Haslam, R. (2004). Expression Profiling of the response of Arabidopsis thaliana to methanol stimulation. Phytochemistry, 65(16), 2305-2316.
Galbally, E. and Kirstine, W. (2002). The production of methanol by flowering plants and the global cycle of methanol. Atmosphere Chemistry, 43(3), 195-229.
Ghafari, H. and Tadayon, M. R. (2018). Impact of jasmonic acid on radiation use efficiency and dry biomasses of sugar beet (Beta vulgaris L.) under water deficit conditions. Plant Productions, 41(4), 111-124. [In Farsi]
Gout, E., Aubert, S., Blingny, R., Rebeille, F., Nonomura, A. R., Benson, A. A. and Douce, R. (2000). Metabolism of methanol in plant cells: Carbon-13 (Nuclear magnetic resonance studies). Plant Physiology, 123(1), 287-296.
Kalidasu, G., Sarada, C. and Reddy, T. Y. (2008). Efficacy of biofertilizers on the performance of rainfed coriander (Coriandrum sativum) in vertisols. Journal of Spices and Aromatic Crops, 17(2), 98-102.
Khosravi, E., Mehrafarin, A. Naghdibadi, H. Hajiabadi, R. and Khosravi, M. (2012). The phytochemical response of (Melissa officinalis L.) to foliar application of hydro-alcoholic solutions (methanol and ethanol). Journal of Herbal Drugs,2(2), 121-28. [In Farsi]
Lawlor, D. (1987). Photosynthesis: Metabolism, control and physiology. Harlow: Longman Sc & Tech.
Lee, Y. J., Kim, N. S., Kim, H., Yi, J. M., Oh, S. M. and Bang, O. S. (2013). Cytotoxic and anti-inflammatory constituents from the seeds of Descurainia sophia. Archives of Pharmacal Research, 36(5), 536-541.
Mehrafarin, A., Naghdi-Badi, H. A., Ghadiri, A. Labafi, M. R., Zend, E. Nourmohammadi, A. H., Qavami, N. and Saif Sahandi, M. (2016). Journal of Medicinal Plants, 4(20), 86-100.
Paknejad, F., Bayat, V., Ardakani, M. R. and Vazan, S. (2012). Effect of methanol foliar application on seed yield and its quality of soybean (Glycine max L.) under water deficit conditions. Annals of Biological Research, 3(5), 2108-2117.
Pasalar, M., Bagheri lankarani, K., Mehrabani, D., Tolide-I, H. R. and Naseri, M. (2013). The effect of descureania sophia l. and prunus domestica l. in prevention of constipation among iranian hajj pilgrims, saudi arabia. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 4(2), 1195-1203.
Sabokrow Foomany, K., Safarzade Vishekaei, M. N. Daneshian, J. and Rangbar, M. (2012). Study of growth and yield indexes of tobacco in response to methanol sprayed. International Journal of Agriculture: Research and Review, 2(3), 149-154.
Safarzadeh Vishekaei, M. N., Mahalleh yosefi, M. and Normohammadi, G. (2011). Effect of spraying methanol on yield of common bean and snap bean. New Topics First National Conference on AgricultureTehran, Iran.
Sani, B. and Aliabadi Farahani, H. (2010). Evaluation of amino acids foliar application on essential oil variations in flixweld (Descurainia sophia L.) under drought stress conditions. USA: American Council for Medicinally Active Plants.
Welch, R. M., Allaway, W. H., House, W. A. and Kubota, J. (1991). Geographical distribution of trace element problems. In J. J. Mortvedt, F. R. Cox, L. M. Shuman and R. M. Welch (Eds.), Micronutrients in agriculture (pp. 31-57). Madison, Wisconsin, USA: Soil Science Society of America, Inc.
 
 © 2020 by the authors. Licensee SCU, Ahvaz, Iran. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-Non Commercial 4.0 International (CC BY-NC 4.0 license) (http://creativecommons.org/licenses/by-nc/4.0/)