Document Type : Research Paper

Authors

1 Assistance Professor, Department of Horticulture, Faculty of Agriculture, Saveh Branch, Islamic Azad University, Saveh, Iran

2 M.Sc. Graduate of Medicinal Plants, Faculty of Agriculture, Saveh Branch, Islamic Azad University, Saveh, Iran

Abstract

Abstract
 
Background and Objectives
Satureja avromanica Maroofi is a medicinally perennial herb which is native to Iran, exclusively restricted to Uramanat mountains of Kurdistan province. It has various pharmaceutical and biological activities such as antibacterial, antifungal, antioxidant, anti-inflammatory, antiviral, anticancer and antiprotozoal properties. The experiment was conducted to study the biochemical characteristics of S. avromanica Maroofi extract by two extraction methods as solvent and solvent-ultrasonic.
 
Materials and Methods
The study was carried out as a factorial based on a completely randomized design. The investigated factors were the type of solvent (water, 75% water, 50% water, 75% methanol and methanol) and extraction method (solvent and solvent-ultrasonic). For this purpose, the plant samples were collected from Uraman Mountains of Kurdistan province in July of 2016. Samples were immediately transferred to Herbarium of Agricultural Research Center and Natural Resources of Kurdistan province. The samples were dried in shade for 15 days, and then crushed into pieces of 0.5 to 1 cm by a laboratory mill (Molyneux, Spain). The content of phenol and flavonoid as well as antioxidant activity was measured. Besides, the components of phenol and flavonoid for the best treatment were determined by HPLC apparatus.
 
Results
The results showed that the compounds extracted by water-ultrasonic were significantly more than those extracted by water. Inhibition percent in water-ultrasonic and water treatments were 16.62% and 11.92%, respectively. The content of phenol (45.36 mg/g, 40.29 mg/g) in water-ultrasonic was higher than that obtained in water treatment (40.29 mg/g). The flavonoid content (15.33 mg/g) in water-ultrasonic was more than that in water treatment (13.29 mg/g). Therefore, water-ultrasonic treatment was more efficient in extracting S. avromanica Maroofi compared to water treatment. On the other hand, the 75% water treatment had more compounds compared to other solvents. The chromatogram of essential ingredient revealed that
S. avromanica Maroofi had more Caffeic acid, Gallic acid, Chlorogenic acid, Quercetin,
p-Coumaric acid, Ferulic acid. Also, Rutin as the highest amount was obtained in Ferulic acid to be 9.12 mg/g.
 
Discussion
We found that water is the best solvent for biochemical extraction of S. avromanica Maroofi. We found a 12% increase of phenol and 14 % increase of flavonoid in water-ultrasonic relative to water treatment. Due to the wide structure of hydrogen bonds, water is a very polar solvent with a dielectric constant at room temperature and atmospheric pressure. Ultrasonic is a type of ultrasound that increases the polarity of the liquid phase. Ultrasonic waves cause mechanical fluctuations in the liquid medium. The mechanical effect of ultrasound improves the penetration of solvent into cellular materials and mass transfer. Hence, ultrasonic waves are more efficient for producing the biochemical compounds like phenol and flavonoid.

Keywords

Main Subjects

References
Askun, T., Tumen, G., Satıl, F. and Karaarslan, D. (2012). Active constituents of some Satureja L. species and their biological activities. African Journal of Microbiology Research, 6(22), 4623-4633.
Blois, M. S. (1985). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199-1200.
Chang, C., Yang, M., Wen, H. and Chern, J. (2002). Estimation of total flavonoid content inpropolis
by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10(3), 178-
182.
Do, Q. D., Angkawijaya, A. E., Tran-Nguyen, P. L., Huynh, L. H., Soetaredjo, F. E., Ismadji, S. and
Ju, Y. H. (2014). Effect of extraction solvent on total phenol content, total flavonoid content,
and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22(3), 296-302.
Ebrahimi, N., Ketabchi, S. and Rowshan, V. (2017). Ntibacterial effect and chemical composition of Satureja bachtiarica. Plant Protection Journal of Islamic Azad Unicerdity of Shiraz, 9(1), 85-106.
[In Farsi]
Ganzera, M., Zhao, J. and Khan, I. A. (2002). Hypericum perforatum-Chemical profiling and quantitative results of St. John's Wort products by an improved high‐performance liquid chromatography method. Journal of Pharmaceutical Sciences, 91(3), 623-630.
Gavara, R., Lima, J. C. and Rodriguez, L. (2016). Effect of solvent polarity on the spectroscopic properties of an alkynyl gold (I) gelator. The particular case of water. Photochemical & Photobiological Sciences, 15(5), 635-643.
Hossain, M. A. and Shah, M. D. (2015). A study on the total phenols content and antioxidant activity of essential oil and different solvent extracts of endemic plant Merremia borneensis. Arabian Journal of Chemistry, 8(1), 66-71.
Karami, Z., Emam Jomeh, Z., Mirzaie, H., Sadeghi A., Khamiri, M. and Aidani, A. (2011). Comparison of Ultrasonic and Soxhlet extraction methods in extraction of phenolic compounds from licorice root. Food Processing and Maintenance Journal, 3(2), 22-1. [In Farsi]
Ma, Y., Ye, X., Hao, Y., Xu, G., Xu, G. and Liu, D. (2008). Ultrasound-assisted extraction of hesperidin from Penggan (Citrus reticulata) peel. Ultrasonics Sonochemistry, 15(3), 227-232.
Maroofi, H. (2010). Two new plant species from Kurdistan province, West of Iran. The Iranian Journal of Botany, 16(1), 76-80. [In Farsi]
Menendez-Baceta, G., Aceituno-Mata, L., Reyes-Garcia, V., Tardio, J., Salpeteur, M. and Pardo-de-Santayana, M. (2015). The importance of cultural factors in the distribution of medicinal
plant knowledge: A case study in four Basque regions. Journal of Ethnopharmacology, 161(4), 116-127.
Metrouh-Amir, H., Duarte, C. M. and Maiza, F. (2015). Solvent effect on total phenolic contents, antioxidant, and antibacterial activities of Matricaria pubescens. Industrial Crops and Products, 67(2), 249-256.
Mirzaee, A., Jaberi, H. and Madani, A. (2011). Antioxidant activities, total phenols and total Flavonoids assay of Origanurm vulgare, Teucrium polium and Thymus daensis. Hormozgan Meficinal Journal, 90(4), 285-294
Mozdasta, Sh., Ebrahimzadeh, M. A. and Eslami, Sh. (2015). Effect of increasing the polarity of solvent on total phenol and flavonoid contents an antioxidant activity of myrtle (Myrtus communis L.). Journal of Mazandaran University of Medicinal Sciences, 25(126), 68-81. [In Farsi]
Mustapa, A. N., Martin, A., Gallego, J. R., Mato, R. B. and Cocero, M. J. (2015). Microwave-
assisted extraction of polyphenols from clinacanthus nutans Lindau medicinal plant: Energy perspective and kinetics modeling. Chemical Engineering and Processing: Process Intensification, 97(4), 66-74.
Nandes, F. A. N., Linhares, F. E. and Rodrigues, S. (2008). Ultrasound as pretreatment for drying of pineapple. Ultrasonics Sonochemistry, 15(6), 1049-1054.
Noshkam, A., Majnoonhoseini, N., Hadian, J., Jahansoz, M. R., Salehnia, A. and Hedaiatpour, S. (2015). The effect of irrigation regimes on qualitative and quantitative yield of Satureja khozestanica and S. Rechingeri in west of Lorestan. Plant Productions, 38(2), 77-88. [In Farsi]
Pourmorad, F., Hosseini Mehr, S. J. and Shahabimaj, D. N. (2006). Antioxida activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African Journal of Biotechnology, 5(11), 1142-1145.
Raza, A., Li, F., Xu, X. and Tang, J. (2017). Optimization of ultrasonic-assisted extraction of antioxidant polysaccharides from the stem of Trapa quadrispinosa using response surface methodology. International Journal of Biological Macromolecules, 94(1), 335-344.
Sadeghi, A., Ebrahimzadeh, A. M., Qurbani, M. and Qarakhabi, M. (2010). Comparison of different methods of extraction of phenolic and flavonoid compounds from Urtica dioica. Research of Iranian Medicinal Plants and Herbs, 26(3), 389-405. [In Farsi]
Sefidkon, F., Askari, F., Sadeghzadeh, L. and olia, P. (2009). Effect of essential oil of Satureja (S. mutica, S. edmondi, S. bachtiarica) on Salmonella paratyphi. Iranian Biology Journal, 22(2), 258-249.
Sherafati-chaleshati, R., Sherafati-chaleshati, F., Sherafati-Chaleshati, A. and Ashrafi, K. (2009). Antimicrobial effect and determination of phenolic and flavonoid compounds of Scrophularia striata ethanol extract. The Journal of Shahrekord University of Medical Sciences, 11(4), 37-32.
Siahmansour, Sh., Ismaili, A. and Nazarian Firouzabadi, F. (2018). Effect of different elicitor treatments on hairy root of medicinal plant poppies (Papaver somniferum L.). Plant Productions, 41(1), 31-42. [In Farsi]
Sultana, B., Anwar, F. and Ashraf, M. )2009(. Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules, 14(6), 2167-2180.
Wojdyło, A., Oszmianski, J. and Czemerys, R. (2007). Antioxidant activity and phenolic compounds in 32 selected herbs. Food chemistry, 105(3), 940-949.
Wu, J., Lin, L. and Chau, F. T. (2001). Ultrasound-assisted extraction of ginseng saponins from ginseng roots and cultured ginseng cells. Ultrasonics Sonochemistry, 8(4), 347-352.
 
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