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بررسی محتوی فنل کل، فلاونوئید کل و ظرفیت آنتی‌اکسیدانی دو گونه وحشی تمشک Rubus persicus و R. caesius در مراحل مختلف رسیدگی میوه

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران

2 کارشناس ارشد علوم گیاهی، دانشکده زیست‌شناسی، پردیس علوم، دانشگاه تهران، تهران، ایران

3 استادیار، گروه علوم گیاهی، دانشکده زیست‌شناسی، پردیس علوم، دانشگاه تهران، تهران، ایران

چکیده

چکیده
آنتی‌اکسیدان‌هابا آزادسازی الکترون‌های منفرد و اتم‌های هیدروژن با تنش اکسیداتیو ناشی از رادیکال‌های آزاد مقابله می‌کنند. مطالعات مختلف نشان داده‌اند که ترکیبات فنلی و فلاونوئیدهای موجود در گیاهان می‌توانند خاصیت آنتی‌‌اکسیدانی داشته باشند. در این آزمایش، عملکرد آنتی‌اکسیدانی، محتوی فنل کل و فلاونوئید کل در مراحل مختلف رسیدگی میوه‌ شامل نارس، نیمه‌رسیده و رسیدگی کامل در دو گونه وحشی تمشک Rubus caesius و R. persicus درخردادماه سال 1392 در آزمایشگاه تحقیقاتی پارک علم و فناوری استان آذربایجان شرقی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی در سه تکرار مقایسه شدندارزیابی شدند. میزان فعالیت آنتی‌اکسیدانی اندازه‌گیری‌شده با روش‌های درصد پاک‌سازی رادیکال آزاد DPPH و توانایی احیای یون آهن فریک (FRAP) در مرحله رسیدگی کامل به‌طور معنی‌داری نسبت به مراحل نارس و نیمه‌رسیده در هر دو گونه وحشی تمشک بیشتر بود. فعالیت آنتی‌اکسیدانی اندازه‌گیری‌شده در مراحل مختلف رسیدگی میوه به ‌استثنای رسیدگی کامل در گونه
R. caesius بیشتر از R. persicusبود. به‌طور مشابه محتوی فنل کل در مراحل مختلف رسیدگی میوه تفاوت معنی‌داری نشان داد به‌طوری‌که مرحله رسیدگی کامل، محتوی فنل کل بالاتری نسبت به سایر مراحل نشان داد. محتوی فلاونوئید کل در مرحله رسیدگی میوه در گونه R. persicusبیشتر از مرحله نارس و نیمه‌رسیده بود ولی این ترکیب در گونه R. caesius در مراحل رسیدگی کامل و نیمه‌رسیده تفاوت معنی‌داری نشان نداد. نتایج این تحقیق نشان می‌دهد که مرحله رسیدگی کامل میوه محتوی فنلی و فلاونوئیدی بالاتری نسبت به مراحل نارس و نیمه‌رسیده دارد که با الگوی تفاوت فعالیت آنتی‌اکسیدانی در بین مراحل رسیدگی مختلف مطابقت دارد. به‌طور مشابه گونه
R. caesius با محتوی فنل کل و فلاونوئید کل بیشتر نسبت به گونه R. persicus، فعالیت آنتی‌اکسیدانی بیشتری نیز نشان می‌دهد.
 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Total Phenolic Content, Total Flavonoids and the Antioxidant Capacity in Two Wild Species of Raspberry, Rubus persicus and R. caesius, at Different Maturity Stages of Fruits

نویسندگان [English]

  • Hamed Dadkhah Aghdash 1
  • Mehdi Daemi Saeidabad 2
  • Mohsen Falahati Anbaran 3

1 Ph.D. Student of Plant Physiology, Department of Plant Sciences, Faculty of Biology science, University of Tarbiat Modares, Tehran, Iran

2 M.Sc. of Plant Sciences, School of Biology, College of Science, University of Tehran, Tehran, Iran

3 Assistant Professor, Department of Plant Sciences, School of Biology, College of Science, University of Tehran, Tehran, Iran

چکیده [English]

Abstract
 
Background and Objectives
Antioxidants can harness the oxidative stress caused by free radicals by releasing single electrons and hydrogen ions. Myriad plants including vegetables and fruits express enzymatic and non-enzymatic antioxidant properties which are used by humans to maintain health.. In this study we aim to compare the level of antioxidants and the amount of total phenolic and flavonoid compounds at three different stages of fruit ripening including immature, semi-ripen and fully matured fruits in two species of raspberry after a period of storage at freezer.
 
Materials and Methods
Fruits of Rubus persicus and R. caesius were collected at different maturity stages including immature, semi-ripen and fully matured stages from natural habitats. The extracts of fruits were stored at -20 °C for 6 months. The antioxidant activities of extracts were determined using DPPH free radical purification and ferric iron regeneration ability (FRAP) methods in June, 2013 in Research Laboratory of Science and Technology Park of East Azerbaijan province. The content of phenolic and flavonoid compounds was also quantified according to Folin–Ciocalteu’s phenol and aluminum chloride methods, respectively. The antioxidant activity and total phenolic and flavonoid contents were compared between species at three maturity stages in a completely randomized factorial design with three replicates.
 
Results
Both methods applied to examine the antioxidant activity revealed a high mean value in extracts from fully matured fruits compared with immature and semi-ripen fruits. The magnitude of antioxidant activity was significantly higher in R. caesius than R. persicus except that the amount measured by DPPH free radical purification level was similar between species at only fully matured stage. A significant difference was observed in both total phenolic and flavonoid contents between immature, semi-ripen and fully matured stages in both species (P < 0.05) except that the total flavonoid content was similar between semi-ripen and fully matured fruits in R. caesius.
 
Discussion
Results showed that the highest antioxidant capacity was found in the fully matured stage of fruits in both species and thus the fully matured fruits might be capable of counteracting and even neutralizing free radicals. The results also showed that different species had different level of antioxidant activity. The level of total phenolic and flavonoid content at fully matured fruits was also higher than immature fruits in both species. Our results suggest that the antioxidant potential of raspberry can have several medicinal applications and play an important role by reducing the oxidative stress.
 

کلیدواژه‌ها [English]

  • DPPH
  • FRAP
  • Free radical
  • Matured stage
  • Rubus caesius
  • Rubus persicus
مراجع
References
Bower, C. (2007). Postharvest handling, storage, and treatment of fresh market berries. In:Yanyun Zhao( Ed.), Berry fruit: Value-added products for health promotion. USA, Corvallis: Taylor &Francis Group, CRC Press.
Brand-Williams, W., Cuvelier, M. E. and Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology, 28(1), 25-30.
Cho, M. J., Howard, L. R., Prior, R. L. and Clark, J. R. (2004). Flavonoid glycosides and
antioxidant capacity of various blackberry, blueberry and red grape genotypes determined by high‐performance liquid chromatography /mass spectrometry. Journal of the Science of Food and Agriculture, 84(13), 1771-1782.
Dai, J., Gupte, A., Gates, L. and Mumper, R. (2009). A comprehensive study of anthocyanin-containing extracts from selected blackberry cultivars: Extraction methods, stability, anticancer properties and mechanisms. Food and Chemical Toxicology, 47(4), 837-847.
Deighton, N., Brennan, R., Finn, C. and Davies, H. V. (2000). Antioxidant properties of domesticated and wild Rubus species. Journal of the Science of Food and Agriculture, 80(9), 1307-1313.
Giovanelli, G. and Buratti, S. (2009). Comparison of polyphenolic composition and antioxidant activity of wild Italian blueberries and some cultivated varieties. Food Chemistry, 112(4), 903-908.
Golestani, A. and Rastegar, S. (2017). Effect of rosemary and artemisia essential oil on decay control and quality characters of mango fruit during storage. Plant Productions, 40(2), 53-62.
Haddadinejad, M., Ghasemi, K. and Mohammadi, A. (2017). Evaluation of temperature and container material effects on summer wild blackberry postharvest life. Plant Productions, 40(2), 99-112.
Hayouni, E. A., Abedrabba, M., Bouix, M. and Hamdi, M. (2007). The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts. Food Chemistry, 105(3), 1126-1134.
Hertog, M. G., Feskens, E. J., Kromhout, D., Hollman, P. and Katan, M. (1993). Dietary antioxidant flavonoids and risk of coronary heart disease: The zutphen elderly study. The Lancet, 342(8878), 1007-1011.
Jennings, D. L. (1988). Raspberries and blackberries: Their breeding, diseases and growth. United States Academic Press.
Joo, M., Lewandowski, N., Auras, R., Harte, J. and Almenar, E. (2011). Comparative shelf life study of blackberry fruit in bio-based and petroleum-based containers under retail storage conditions. Food Chemistry, 126(4), 1734-1740.
Kaijv, M., Sheng, L. and Chao, C. (2006). Antioxidation of flavonoids of green rhizome. Food Science, 27(2), 110-115.
Kim, B., Kim, J., Kim, H. and Heo, M. (1997). Biological screening of 100 plant extracts for cosmetic use (II): antioxidative activity and free radical scavenging activity. International Journal of Cosmetic Science, 19(6), 299-307.
Kim, H. S., Park, S. J., Hyun, S. H., Yang, S. O., Lee, J., Auh, J. H., Kim, J. H., Cho, S. M., Marriott, P. J. and Choi, H. K. (2011). Biochemical monitoring of black raspberry (Rubus coreanus Miquel) fruits according to maturation stage by 1H NMR using multiple solvent systems. Food Research International, 44(7), 1977-1987.
Kordali, S., Cakir, A., Mavi, A., Kilic, H. and Yildirim, A. (2005). Screening of chemical composition and antifungal and antioxidant activities of the essential oils from three Turkish Artemisia species. Journal of Agricultural and Food Chemistry, 53(5), 1408-1416.
Lahlou, M. (2004). Methods to study the phytochemistry and bioactivity of essential oils. Phytotherapy Research, 18(6), 435-448.
Lee, S. E., Hwang, H. J., Ha, J. S., Jeong, H. S. and Kim, J. H. (2003). Screening of medicinal plant extracts for antioxidant activity. Life Sciences, 73(2), 167-179.
Liu, R. H. (2003). Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. The American Journal of Clinical Nutrition, 78(3), 517S-520S.
Prior, R. L., Cao, G., Martin, A., Sofic, E., McEwen, J., O'Brien, C., Lischner, N., Ehlenfeldt, M., Kalt, W. and Krewer, G. (1998). Antioxidant capacity as influenced by total phenolic and anthocyanin content, maturity, and variety of Vaccinium species. Journal of Agricultural and Food Chemistry, 46(7), 2686-2693.
Rasheed, R., Ali, B. and Bashir, A. (1995). Effect of Teucrium stocksianum on paracetamol-induced hepatotoxicity in mice. General Pharmacology: The Vascular System, 26(2), 297-301.
Rezai Ghivi, A. (2013). Physiological and biochemical study of fruit of four Iranian raspberry species during ripening and post harvest. Ph.D. Thesis, Azad University Research Sciences Branch, Ahvaz. [In Farsi]
Ryu, J., Kwon, S. J., Jo, Y. D., Jin, C. H., Nam, B. M., Lee, S. Y., Jeong, S. W., Im, S. B., Oh, S. C. and Cho, L. (2016). Comparison of phytochemicals and antioxidant activity in blackberry (Rubus fruticosus L.) fruits of mutant lines at the different harvest time. Plant Breeding and Biotechnology, 4(2), 242-251.
Tulio Jr, A. Z., Reese, R. N., Wyzgoski, F. J., Rinaldi, P. L., Fu, R., Scheerens, J. C. and Miller, A. R. (2008). Cyanidin 3-rutinoside and cyanidin 3-xylosylrutinoside as primary phenolic antioxidants in black raspberry. Journal of Agricultural and Food Chemistry, 56(6), 1880-1888.
Willett, W. C. (1994). Micronutrients and cancer risk. The American Journal of Clinical Nutrition, 59(5), 1162-1165.
Zeidan, R. and Oran, S. (2014). Antioxidant activity of leaf and fruit extracts of Jordanian Rubus sanguineus Friv. (Rosaceae). Journal of Medicinal Plants Research, 8(39), 1179-1190.
 
 © 2019 by the authors. Licensee SCU, Ahvaz, Iran. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-Non Commercial 4.0 International (CC BY-NC 4.0 license) (http://creativecommons.org/licenses/by-nc/4.0/)
تولیدات گیاهی
دوره 42، شماره 3
آذر 1398
صفحه 295-306
فایل ها
هم رسانی
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