بررسی اثر غلظت‌های متفاوت سدیم نیتروپروساید در تخفیف صدمات اکسایشی ناشی از تنش آبی حاصل از پلی‌اتیلن‌گلایکول در گیاه دارویی پونه‌سا بی‌کرک در شرایط درون شیشه‌ای

نوع مقاله: علمی - پژوهشی

نویسندگان

1 دانشجوی کارشناس ارشد علوم باغبانی-گیاهان دارویی، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

2 دانشیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

3 دانشجوی کارشناسی مهندسی کشاورزی، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

امروزه کاربرد سدیم نیتروپروساید به‌عنوان آنتی‌اکسیدان و تنظیم‌کننده رشد گیاهی به‌منظور کاهش اثرات منفی ناشی از تنش‌های مختلف مطرح می‌باشد. جهت بررسی این موضوع آزمایشی به‌صورت فاکتوریل در قالب طرح کاملأ تصادفی در 5 تکرار در شرایط درون شیشه‌ای در آزمایشگاه دانشکده کشاورزی دانشگاه فردوسی مشهد انجام شد. فاکتور اول تنش آبی حاصل از پلی‌اتیلن‌گلایکول (PEG-6000) در سه سطح (صفر، 5/0 و 1 درصد) و فاکتور دوم غلظت‌های مختلف سدیم نیتروپروساید (صفر، 25، 50 و 100 میکرومولار) در محیط پایه MS بود. جهت تهیه ریز نمونه، بذور پونه‌سا بی‌کرک پس از ضدعفونی در محیط پایه MS کشت شدند. ریز نمونه‌ها پس از رشد تحت تأثیر پلی‌اتیلن‌گلایکول و غلظت‌های مختلف سدیم نیتروپروساید موجود در محیط پایه MS قرار گرفتند. بعد از دو هفته میزان رشد، فعالیت آنتی‌اکسیدانی، فنل کل و نشت یونی در نمونه‌ها مورد ارزیابی قرار گرفت. تنش آبی، صفات مربوط به رشد ریز نمونه‌ها شامل؛ ارتفاع گیاهچه، فاصله میانگره، وزن‌ تر گیاهچه، تعداد گره و تعداد شاخه در هر گیاهچه را کاهش داد. کاربرد غلظت 25 میکرومولار سدیم نیتروپروساید تأثیر بسزایی در بهبود این صفات داشت، به‌طوری‌که بیشترین مقادیر صفات فوق مربوط به تیمار عدم تنش آبی و کاربرد این غلظت سدیم نیتروپروساید بود. هم‌چنین بیشترین فعالیت آنتی‌اکسیدانی (15/85 درصد)، میزان فنل‌کل (15/5 میلی‌گرم بر گرم وزن تر) و نشت یونی (92/74 درصد) در تنش آبی حاصل از یک درصد پلی‌اتیلن‌گلایکول و عدم کاربرد تخفیف‌دهنده سدیم نیتروپروساید حاصل شد. با کاربرد سدیم نیتروپروساید این صفات بیوشیمیایی کاهش یافتند. نتایج این تحقیق نشان داد کاربرد سدیم نیتروپروساید با غلظت مناسب، می‌تواند در کاهش اثرات سوء تنش آبی در گیاه پونه‌سا بی‌کرک مفید باشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Effect of Different Concentrations of Sodium Nitroprusside in Alleviating Oxidative Damages Caused by Water Stress of Polyethylene Glycol in Medicinal Plant of Catmint Hairless under In Vitro Condition

نویسندگان [English]

  • R. Narimani 1
  • M. Moghaddam 2
  • D. Shokouhi 3
1 M.Sc. Student of Horticulture Science-Medicinal Plant, Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
2 Associate Professor, Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
3 B.Sc. Student of Agriculture Engineering, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده [English]

Abstract
Background and Objectives
Nowadays, the use of sodium nitroprusside as an antioxidant and plant growth regulators to decrease the negative effects of various stresses has been an in portant consideration.
Materials and Methods
This investigation was conducted as a factorial experiment based on a completely randomized design with five replications at the laboratory of Faculty of Agriculture, Ferdowsi University of Mashhad. The first factor was water stress in three levels (0, 0.5, 1 %) by polyethylene glycol (PEG), and the second factor was use of different concentrations of sodium nitroprusside (SNP) (0, 25, 50, 100 µM), in MS medium. In order to provide the explants, seeds of catmint hairless were disinfected and cultivated in MS medium. When the explants were grown, they were affected by polyethylene glycol and different concentrations of sodium nitroprusside in MS medium. After two weeks, growth characteristics, antioxidant activity, total phenol content and electrical conductivity were measured in explants.
Results
Water stress reduced the traits related to explants growth such as explants height, distance between internodes, fresh weight of explants, number of nodes and number of branches in each explant. Concentration of 25 µM of sodium nitroprusside had a great impact on improving these traits. The maximum amount of the measured traits was observed in water-stress-conditions using 25 µM of sodium nitroprusside and in without-water-stress conditions. Also, antioxidant activity (85.15%), total phenol content (5.15 mg/g FW) and electrical conductivity (74.92%) were in maximum amount in water-stress conditions by 1% polyethylene glycol application and non-use of sodium nitroprusside that decreased with the use of sodium nitroprusside.
Discussion
The results showed that using NO as an antioxidant compound reduces the effects of tension generally and the low concentration of nitric oxide (Concentration of 25 µM of SNP) seems to be the best concentration for catmint hairless seedlings under severe drought stress conditions which can reduces the adverse effects of stress and increases the probability of tolerance of seedlings in drought stress conditions by effecting on antioxidant activity of the plant.

کلیدواژه‌ها [English]

  • antioxidant activity
  • Free Radical
  • Growth Characteristics
  • Nitric oxide
  • Oxidative Stress
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