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بررسی اثر غلظت‌های متفاوت سدیم نیتروپروساید در تخفیف صدمات اکسایشی ناشی از تنش آبی حاصل از پلی‌اتیلن‌گلایکول در گیاه دارویی پونه‌سا بی‌کرک در شرایط درون شیشه‌ای

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشجوی کارشناس ارشد علوم باغبانی-گیاهان دارویی، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

2 دانشیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

3 دانشجوی کارشناسی مهندسی کشاورزی، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

امروزه کاربرد سدیم نیتروپروساید به‌عنوان آنتی‌اکسیدان و تنظیم‌کننده رشد گیاهی به‌منظور کاهش اثرات منفی ناشی از تنش‌های مختلف مطرح می‌باشد. جهت بررسی این موضوع آزمایشی به‌صورت فاکتوریل در قالب طرح کاملأ تصادفی در 5 تکرار در شرایط درون شیشه‌ای در آزمایشگاه دانشکده کشاورزی دانشگاه فردوسی مشهد انجام شد. فاکتور اول تنش آبی حاصل از پلی‌اتیلن‌گلایکول (PEG-6000) در سه سطح (صفر، 5/0 و 1 درصد) و فاکتور دوم غلظت‌های مختلف سدیم نیتروپروساید (صفر، 25، 50 و 100 میکرومولار) در محیط پایه MS بود. جهت تهیه ریز نمونه، بذور پونه‌سا بی‌کرک پس از ضدعفونی در محیط پایه MS کشت شدند. ریز نمونه‌ها پس از رشد تحت تأثیر پلی‌اتیلن‌گلایکول و غلظت‌های مختلف سدیم نیتروپروساید موجود در محیط پایه MS قرار گرفتند. بعد از دو هفته میزان رشد، فعالیت آنتی‌اکسیدانی، فنل کل و نشت یونی در نمونه‌ها مورد ارزیابی قرار گرفت. تنش آبی، صفات مربوط به رشد ریز نمونه‌ها شامل؛ ارتفاع گیاهچه، فاصله میانگره، وزن‌ تر گیاهچه، تعداد گره و تعداد شاخه در هر گیاهچه را کاهش داد. کاربرد غلظت 25 میکرومولار سدیم نیتروپروساید تأثیر بسزایی در بهبود این صفات داشت، به‌طوری‌که بیشترین مقادیر صفات فوق مربوط به تیمار عدم تنش آبی و کاربرد این غلظت سدیم نیتروپروساید بود. هم‌چنین بیشترین فعالیت آنتی‌اکسیدانی (15/85 درصد)، میزان فنل‌کل (15/5 میلی‌گرم بر گرم وزن تر) و نشت یونی (92/74 درصد) در تنش آبی حاصل از یک درصد پلی‌اتیلن‌گلایکول و عدم کاربرد تخفیف‌دهنده سدیم نیتروپروساید حاصل شد. با کاربرد سدیم نیتروپروساید این صفات بیوشیمیایی کاهش یافتند. نتایج این تحقیق نشان داد کاربرد سدیم نیتروپروساید با غلظت مناسب، می‌تواند در کاهش اثرات سوء تنش آبی در گیاه پونه‌سا بی‌کرک مفید باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Different Concentrations of Sodium Nitroprusside in Alleviating Oxidative Damages Caused by Water Stress of Polyethylene Glycol in Medicinal Plant of Catmint Hairless under In Vitro Condition

نویسندگان [English]

  • R. Narimani 1
  • M. Moghaddam 2
  • D. Shokouhi 3

1 M.Sc. Student of Horticulture Science-Medicinal Plant, Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

2 Associate Professor, Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

3 B.Sc. Student of Agriculture Engineering, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

چکیده [English]

Abstract
Background and Objectives
Nowadays, the use of sodium nitroprusside as an antioxidant and plant growth regulators to decrease the negative effects of various stresses has been an in portant consideration.
Materials and Methods
This investigation was conducted as a factorial experiment based on a completely randomized design with five replications at the laboratory of Faculty of Agriculture, Ferdowsi University of Mashhad. The first factor was water stress in three levels (0, 0.5, 1 %) by polyethylene glycol (PEG), and the second factor was use of different concentrations of sodium nitroprusside (SNP) (0, 25, 50, 100 µM), in MS medium. In order to provide the explants, seeds of catmint hairless were disinfected and cultivated in MS medium. When the explants were grown, they were affected by polyethylene glycol and different concentrations of sodium nitroprusside in MS medium. After two weeks, growth characteristics, antioxidant activity, total phenol content and electrical conductivity were measured in explants.
Results
Water stress reduced the traits related to explants growth such as explants height, distance between internodes, fresh weight of explants, number of nodes and number of branches in each explant. Concentration of 25 µM of sodium nitroprusside had a great impact on improving these traits. The maximum amount of the measured traits was observed in water-stress-conditions using 25 µM of sodium nitroprusside and in without-water-stress conditions. Also, antioxidant activity (85.15%), total phenol content (5.15 mg/g FW) and electrical conductivity (74.92%) were in maximum amount in water-stress conditions by 1% polyethylene glycol application and non-use of sodium nitroprusside that decreased with the use of sodium nitroprusside.
Discussion
The results showed that using NO as an antioxidant compound reduces the effects of tension generally and the low concentration of nitric oxide (Concentration of 25 µM of SNP) seems to be the best concentration for catmint hairless seedlings under severe drought stress conditions which can reduces the adverse effects of stress and increases the probability of tolerance of seedlings in drought stress conditions by effecting on antioxidant activity of the plant.

کلیدواژه‌ها [English]

  • antioxidant activity
  • Free Radical
  • Growth Characteristics
  • Nitric oxide
  • Oxidative Stress
مراجع
References
Bhatt, R.M. and Srinivasa Rao, N.K. (2005). Influence of pod load on response of okra to water stress. Indian Journal of Plant Physiology, 10(1): 54-59.
Corchete, P. and Guerra, H. (1986). Effect of NaCl and polyethylene glycol on solute content and glycosidase activities during germination of lentil seeds. Plant, Cell & Environment, 9(7): 589-593.
Dami, I. and Hughes, H. (1995). Leaf anatomy and water loss of in vitro PEG-treated ‘Valiant’grape. Plant Cell, Tissue and Organ Culture, 42(2): 179-184.
Del Rio, L.A., Corpas, F.J., and Barroso, J.B. (2004). Nitric oxide and nitric oxide synthase activity in plants. Phytochemistry, 65(7): 783-792.
Duan, X., Su, X., You, Y., Qu, H., Li, Y., and Jiang, Y. (2007). Effect of nitric oxide on pericarp browning of harvested longan fruit in relation to phenolic metabolism. Food Chemistry, 104(2): 571-576.
Estill, K., Delaney, R.H., Smith, W.K., and Ditterline, R.L. (1991). Water relations and productivity of alfalfa leaf chlorophyll variants. Crop Science. 31(5): 1229-1233.
Fraga, B.M., Hernández, M.G., Mestres, T., and Arteaga, J. (1998). Abietane diterpenes from Nepeta teydea. Phytochemistry, 47(2): 251-254.
Fu, J. and Huang, B. (2001). Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environmental and Experimental Botany, 45(2): 105-114.
Gabler, J. (2002). Drought stress and nitrogen effects on Coriandrum sativum L. Journal of Herbs, Spices & Medicinal Plants, 44: 12-28.
Georgieva, M., Djilianov, D., Konstantinova, T., and Parvanova, D. (2004). Screening of Bulgarian raspberry cultivars and elites for osmotic tolerance in vitro. Biotechnology & Biotechnological Equipment, 18(2): 95-98.
Han, X., Yang, H., Duan, K., Zhang, X., Zhao, H., You, S., and Jiang, Q. (2009). Sodium nitroprusside promotes multiplication and regeneration of Malus hupehensis in vitro plantlets. Plant Cell, Tissue and Organ Culture, 96(1): 29-34.
Hasani, A. (2005). Effect of water stress induced from polyethylene glycol (PEG) on germination factors of basil (Ocimum basillicum). Iranian Journal of Medicinal and Aromatic Plants, 21(4): 535- 543.
Hayat, S., Hasan, S.A., Mori, M., Fariduddin, Q., and Ahmad, A., (2010). Nitric oxide: chemistry, biosynthesis, and physiological role. In: Nitric Oxide in Plant Physiology. pp: 1-15.
Hung, KT. and Kao CH. (2003). Nitric oxide counteracts the senescence of rice leaves induced by abscisic acid. Plant Physiology, 160(8): 871-879.
Jamzad, Z., Grayer, R.J., Kite, G.C., Simmonds, M.S., Ingrouille, M., and Jalili, A. (2003). Leaf surface flavonoids in Iranian species of Nepeta (Lamiaceae) and some related genera. Biochemical Systematics and Ecology, 31(6): 587-600.
Katerji, N., Van Hoorn, J.W., Hamdy, A., Mastrorilli, M., and Karzel, E.M. (1997). Osmotic adjustment of sugar beets in response to soil salinity and its influence on stomatal conductance, growth and yield. Agricultural Water Management, 34(1): 57-69.
Khazai, H. (2002). Effect of drought stress on yield and physiological characteristics of resistance and sensitive varieties of wheat and introducing the best indicator of drought stress resistance. Crop Physiology Ph.D Thesis. Faculty of Agriculture, Ferdowsi University of Mashhad. [in Farsi].
Liu, X., Wang, L., Liu, L., Guo, Y., and Ren, H., 2013. Alleviating effect of exogenous nitric oxide in cucumber seedling against chilling stress. African Journal of Biotechnology. 10(21): 4380-4386.
Lutts, S., Kinet, J.M., and Bouharmont, J. (1995). Changes in plant response to NaCl during development of rice (Oryza sativa L.) varieties differing in salinity resistance. Journal of Experimental Botany, 46(12): 1843-1852.
Maity, S., Chatterjee, S., Variyar, P.S., Sharma, A., Adhikari, S., and Mazumder, S. (2013). Evaluation of antioxidant activity and characterization of phenolic constituents of Phyllanthus amarus root. Journal of Agricultural and Food Chemistry, 61(14): 3443-3450.
Molassiotis, A.N., Sotiropoulos, T., Tanou, G., Kofidis, G., Diamantidis, G., and Therios, E. (2006). Antioxidant and anatomical responses in shoot culture of the apple rootstock MM 106 treated with NaCl, KCl, mannitol or sorbitol. Biologia Plantarum, 50(1): 61-68.
Moon, J.H. and Terao, J. (1998). Antioxidant activity of caffeic acid and dihydrocaffeic acid in lard and human low-density lipoprotein. Journal of Agricultural and Food Chemistry, 46(12): 5062-5065.
Mozaffarian, V.A. (2006). Dictionary of Iranian Plant Names: Latin-English-Persian. 4th Ed. Farhang Moaser. Tehran. pp: 360. [In Farsi]
Muchuweti, M., Kativu, E., Mupure, C.H., Chidewe, C., Ndhlala, A.R., and Benhura, M.A.N. (2007). Phenolic composition and antioxidant properties of some spices. American Journal of Food Technology, 2(5): 414-420.
Neill, S., Barros, R., Bright, J., Desikan, R., Hancock, J., Harrison, J., Morris, P., Ribeiro, D., and Wilson, I. (2008). Nitric oxide, stomatal closure, and abiotic stress. Journal of Experimental Botany, 59(2):165-176.
Neill, S.J., Desikan, R., and Hancock, J.T. (2003). Nitric oxide signaling in plants. New Phytologist, 159(1): 11-35.
Shi, Q., Ding, F., Wang, X., and Wei, M., (2007). Exogenous nitric oxide protects cucumber roots against oxidative stress induced by salt stress. Plant Physiology and Biochemistry, 45: 542-550.
Singleton, V.L. and Rossi, J.A., (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3): 144-158.
Sofo, A., Tuzio, A.C., Dichio, B., and Xiloyannis, C. (2005). Influence of water deficit and rewatering on the components of the ascorbate-glutathione cycle in four interspecific Prunus hybrids. Plant Science, 169(2): 403-412.
Takeda, Y., Ooiso, Y., Masuda, T., Honda, G., Otsuka, H., Sezik, E., and Yesilada, E. (1998). Iridoid and eugenol glycosides from Nepeta cadmea. Phytochemistry, 49(3): 787-791.
Tan, B.C., Chin, C.F., and Alderson, P. (2013). Effects of sodium nitroprusside on shoot multiplication and regeneration of Vanilla planifolia Andrews. In Vitro Cellular & Developmental Biology-Plant, 49(5): 626-630.
Tan, J., Zhao, H., Hong, J., Han, Y., Li, H., and Zhao, W. (2008). Effects of exogenous nitric oxide on photosynthesis, antioxidant capacity and proline accumulation in wheat seedlings subjected to osmotic stress. World Journal of Agricultural Science, 4(3): 307-313.
Taskina, A., Javan, M., Sonboli, A., and Semnanian, S. (2012). Antinociceptive and anti-inflammatory activities of the essential oil of Nepeta crispa Willd. in experimental rat models. Natural Product Research, 26(16): 1529-1534.
Wang, J.W., and Wu, J.Y. (2005). Nitric oxide is involved in methyl jasmonate-induced defense responses and secondary metabolism activities of Taxus cells. Plant and Cell Physiology, 46(6): 923-930.
Wu, C.H., Tewari, R.K., Hahn, E.J., and Paek, K.Y. (2007). Nitric oxide elicitation induces the accumulation of secondary metabolites and antioxidant defense in adventitious roots of Echinacea purpurea. Journal of Plant Biology, 50(6): 636-643.
Zhao, J., Davis, L.C., and Verpoorte, R. (2005). Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnology Advances, 23(4): 283-333.
 
تولیدات گیاهی
دوره 40، شماره 3
آذر 1396
صفحه 77-88
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هم رسانی
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