Document Type : Research Paper

Author

Assistant Professor, Department of Horticultural Sciences Engineering, Faculty of Agriculture and Plant Resources, Imam Khomeini International University, Qazvin, Iran

Abstract

Abstract
Background and Objectives
Any improvement in agricultural system that results in higher production should reduce the negative environmental impact of agriculture and enhance the sustainability of the system. Seaweed extracts (SWE) are known to cause many beneficial effects on plants as they contain growth promoting hormones, trace elements, vitamins and amino acids. Humic acid plays a vital role in soil fertility and its application increases the plant growth and nutrient uptake. The present study was undertaken to investigate the effect of SWE, humic acid and chemical fertilizers on the morphological and biochemical characteristics of Trigonella foenum-graecum L.
Material and methods
The experiment was carried out in 2016 at the Agricultural Research Greenhouse at Imam Khomeini International University, Qazvin, Iran. The experiment was based on a completely randomized design with four treatments including: SWE, humic acid, chemical fertilizers and control and 7 replications. The pots were filled with 5kg of farm soil, which was evenly mixed with recommended level of chemical fertilizers (N; 0.08, P; 0.06 and K; 0.1 g/kg soil) for chemical fertilizer treatment. Seeds were sown at a depth of 1.5 cm in each pot. Seedlings were thinned to five plants per pot 10 days after emergence.  In 4 leaves, appearance stage, SWE and humic acid treatments were applied as a foliar spray every 14 days, for three times before harvesting. Growth parameters were measured in correct time. The biochemical constituent pigments such as chlorophylls and carotenoids and also total ascorbic acid in leaf were estimated in control and treated plants using standard methods. Dried seeds of Fenugreekwere subjected to hydro distillation for 3h using a Clevenger-type apparatus to produce oil and essential oil percentage was measured.
Results
In relation to the mean comparisons, the maximum shoot length and the highest number of pods per plant was obtained in plants receiving SWE and NPK. Maximum plant fresh and dry weights were observed in plants received SWE. All fertilizer treatments increased the number of seeds per pod but there were no significant differences between the treatments. The highest chlorophyll b, total chlorophyll contents, total carotenoids, ascorbic aicd and essential oil percent were obtained in plants that received SWE.
Discussion
Foliar application of seaweed extract fertilizer on fenugreek plants significantly increased all the examined growth parameters of the plants compared to the control. These results agree with previous studies where growth and plant vigor of dill, coriander and fenugreek plants were enhanced by seaweed extracted treatment. Seaweed extracts create equilibrium in growth as a result of the present auxins which will increase vitamins and hormones produced in the treated plants. Results showed that treatments had a significant effect on total chlorophyll, chlorophyll 'a' and 'b', total carotenoids and essential oil content of fenugreek. Seaweed extracts contain cytokinins as well which induce the physiological activities and increase the total chlorophyll, carotenoids and ascorbic acid in the plant.
 

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Main Subjects

References
Adams-Phillips, L., Barry, C., and Giovannoni, J. (2004). Signal transduction systems regulating fruit ripening. Trends Plant Sciences, 9: 331-338.
Akbarinia, A., Jahanfar, D., and Beygif arzad, M. (2007). Effect of nitrogen fertilizer and plant density on seed yield, essential oil and fixed oil content of Coriandrum sativum L. International Journal Medicinal Aromatic Plants, 22(34): 410-419.
Arthur, G.D., Stirk, W.A., and Staden, J. (2003). Effect of a seaweed concentrate on the growth and yield of three varieties of Capsicum annuum. South Africa Journal Botany, 69: 207-211.
Ashraf, M., Ali, Q., and Iqba, Z. (2006). Effect of nitrogen application rate on the content and composition of oil, essential oil and minerals in Nigella sativa L. seeds. Journal Science Food Agriculture, 8630(60): 871-876.
Astaraei, A.R. and Ivani, R. (2008). Effect of organic sources as foliar spray and root media on nutrition if cowpea plant. American-Eurasian Journal of Agriculture Environmental Science, 3(3): 352-356.
Biggs, R. H., Obreza, T. A., and Webb, R. G. (1989). Humate material: Their effect and use as soil amendment. Journal of Applied Phycology, 5: 115-120.
Blunden, G., Jenkins, T., and Liu, H. (1996). Enhanced leaf chlorophyll levels in plants treated with seaweed extract. Journal of Applied Phycology, 8: 535-543.
Crouch, I. and Staden, J. (1992). Effect of seaweed concentrates on the establishment and yield of greenhouse tomato plants. Journal of Applied Phycology, 4: 291-296.
Dere, S., Gunes, T., and Sivci, R. (1998). Spectrophotometric determination of chlorophylla, b and total carotenoid contents of some algae species using different solvents. Turkish Journal of Botany, 22: 13-17.
Dini, M. (2006). Scientific name of medicinal plants used in traditional medicine. Forest and Rangeland Research Institute Publication. Iran, pp: 299-300. [In Farsi]
El-Bassiony, A.M., Fawzy, Z.F., Abd El-Baky, M.M.H., and Asmaa, R.M. (2010). Response of snap bean plants to mineral fertilizers and humic acid application. Research Journal of Agriculture and Biological Sciences, 6(2): 169-175.
Eris, A., Sirritepe, H.O., and Sirritepe, N. (2008). The effect of seaweed (Ascophyllum nodosum) Extract on yield and quality criteria in peppers. Acta Horticulturae, 412: 733-737.
Ghorbani, S., Khazaei, H., Kafi, M., and Banayan aval, M. (2010). Effect of using humic in irrigation water on corn yield. Journal of Agricultural Ecology, 2(1): 111-118. [In Farsi]
Haghparast, M., Molki Farahani, S., Sinki, M., and Zarei, Gh. (2012). Reducing negative effects of water stress in peas with the use of humic acid and seaweed extract. Crop Production under Environmental Stresses, 4(1): 59-71.
Hassanpour aghdam, M.B., Tabatabaie, S.J., Nazemiyeh, H., and Aflatuni, A. (2008). N and nutrition levels affect growth and essential oil content of costmary (Tanacetum balsamita L.). Food Agriculture and Environment, 6(2):150-159.
Heidari, M. and  Khahlil, S. (2014).  Effect of humic acid and phosphorus fertilizer on seed and flower yield, photosynthetic pigments and mineral elements concentration in sour tea (Hisbiscus sabdariffa L.). Iranian Journal of Field Crop Science, 45(2): 191-199. [In Farsi]
Hellal, F.A., Mahfouz, S.A., and Hassan, F.A.S. (2011). Partial substitution of mineral nitrogen fertilizer by bio-fertilizer on Anethum graveolens plant. Agricultural Biology Journal of North America, 4: 652-660.
Kingman, A. R. and Moore, J. (1982). Isolation, purification and quantification of several growth regulating substance in Ascophyllum nodosum (Phaeophyceae). Botanica Marin, 25: 149-153.
Kirshmamoorthy, V. and Madalager, M.B. (2000). Effect of interaction of nitrogen and phosphorus on seed and essential oil of ajowan (Trachypermum ammi). Journal of Spices and Aromatic Crop, 9: 137-139.
Klein, B.P. and Perry, A.K. (1982). Ascorbic-acid and vitamin-A activity in selected vegetables from different geographical areas of the United States. Journal of Food Science, 47(3): 941-945.
Kocira, A., Kornas, R., and Kocira, S. (2013). Effect assessment of Kelpak sl on the Bean yield. Journal of Central European Agriculture, 14(2): 67-76.
Ludwig-Muller, J. (2000). Indole-3-butyric acid in plant growth and development. Plant Growth Regulation, 2-3: 219-230.
Mandegary, A., Pournamdari, M., Sharififar, F., Pournourmohammadi, Sh., Fardiar, R., and Shooli, S. (2012). Alkaloid and flavonoid rich fractions of fenugreek seeds (Trigonella foenum-graecum L.) With antinociceptive and anti-inflammatory effects. Journal Food and Chemical Toxicology, 50: 2503-2507.
Manisha, P. and Angoorbala, B. (2013). Effect of sewage on growth parameters and chlorophyll content of Trigonella foenumgraecum (Methi). International Research Journal of Environment Sciences, 2(9): 5-9.
Mirhashemi, S. M., Koocheki, A., Parsa, M., and Nassiri Mahallati, M. (2009). Evaluating the benefit of Ajowan and Fenugreek intercropping in different levels of manure and planting pattern. Journal of Iranian Field Crop Research, 7(1): 269-279. [In Farsi]
Naderi, S., Pizzeghello, D., Muscolo, A., and Vianello, A. (2002). Physiological effects of humic substances on higher plants. Soil Biology and Biochemistry, 34: 1527-1536.
Pise, N.M. and Sabale, A.B. (2010). Effect of seaweed concentrates on the growth and biochemical constituents of Trigonella foenum-graecum L. Journal of Phytology, 2(4): 50-56.
Poozeshi, R., Zabihi, H., Ramezani Moghadam, M., Rajabzadeh, M., and Mokhtari, A. (2011). The effect of foliar application of humic acid and acetic acid on yield, yield components and concentration of elements in grape varieties Peikani. Horticultural Sciences, 3(25): 351-360.
Sabzevari, S. and Khazaie, H.R. (2009). The effect of foliar application with humic acid on growth, yield and yield components of wheat (Triticum aestivum L.). Agroecology, 2(1): 53-63. [In Farsi]
Sabzevari, S., Khazaie, H.R., and Kafi, M. (2010). Study on the effects of humic acid on germination of four wheat cultivars (Triticun aestivum L.). Journal of Iranian Field Crop Research, 8(3): 473-480. [In Farsi]
Shahbazi, F., Seyyed nejad, F., Salimi, M., and Gilani, A. (2015). Effect of seaweed extracts on the growth and biochemical constituents of wheat. International Journal of Agriculture and Crop Sciences, 8(3): 283-287.
Sivasangari, R., Nagaraj, S., and Vijayanand, N. (2011). Influence of seaweed liquid extracts on growth, biochemical and yield characteristics of Cyamopsis tetragonolaba (L.) Taub. Journal of Phytology, 3(9); 37-41.
Stirk, W. and Staden, J. (1997). Isolation and identification of cytokinins in a new commercial seaweed product made from Fucus serratus L. Journal of Applied Phycology, 9: 327-330.
Taghadosi, M., Hasani, N., and Sinki, J. (2012). Irrigation stress and spraying with humic acid and seaweed extract in antioxidant enzymes and proline in sorghum. Crop Production under Environmental Stresses, 4(1):1-12.
Tahami, S.M.K. (2010). Evaluation of the effects of organic, biological and chemical fertilizers on yield, yield components and essential oil of basil (Ocimum basilicum L.). M.Sc. Thesis, Faculty Agriculture, Ferdowsi Universuty of Mashhad, Iran. [In Farsi]
Thambiraj, J, Lingakumar, K, and Paulsamy, S. (2012). Effect of seaweed liquid fertilizer (SLF) prepared from Sargassum wightii and Hypnea musciformis on the growth and biochemical constituents of the pulse, Cyamopsis tetragonoloba (L). Journal of Research in Agriculture, 1: 065-070.
Thirumaran, G., Anantharaman, P., and Kannan, L. (2007). Effect of seaweed liquid fertilizer in the radish (Raphanus sativus). Journal of Ecobiology, 20(1): 49-52.
Thirumaran, G., Arumugam, M., Arumugam, R., and Anantharaman, P. (2009). Effect of seaweed liquid fertilizer on growth and pigment concentration of Cyamopsis tetrogonolaba (L) Taub. American-Eurasian Journal of Agronomy, 2(2): 50-56.
Thirumaran, G., Pratap Karmakar, P., and Anantharaman, A. (2006). Efffect of seaweed extracts used as fertilizer for Abelmoschus esculentus. Journal of Ecobiology, 19(4): 373-376.
Verkleij, F.N. (1992). Seaweed extract in agriculture and horticulture. Review Biology Agriculture, 8: 309-334.
Whapham, C., Blunden, G., Jenkins, T., and Hankins, S. (1993). Significance of betaines in the increased chlorophyll content of plants treated with seaweed extract. Journal of Applied Phycology, 5: 231-234.
Wu, S.C., Caob, Z.H., Lib, Z.G., Cheunga, K.C., and Wong, M.H. (2005). Effects of biofertilizers containing N-fixer, P and K solubilizers and AM fungi on maize growth: A greenhouse trial. Geoderma, 125: 155-166.
Zhang, X. (1997). Influence of plant growth regulators on turfgrass growth, antioxidant status, and drought tolerance. Virginia Polytechnic Institute and State University, USA. P. 131.
Zodape, S.T., Gupta, A., Bhandari, S.C., Rawat, U.S., Chaudhary, D.R., and Eswaran, K. (2011). Foliar application of seaweed sap as biostimulant for enhancement of yieldand quality of tomato (Lycopersicon esculentum Mill.). NISCAIR-CSIR, India. pp: 215-219.