ارزیابی تنوع ژنتیکی برخی توده‌های هندوانه بومی ایران (Citrullus vulgaris) در شرایط آب و هوایی ارومیه

نوع مقاله: علمی - پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد تولیدات گیاهی، گروه تولیدات گیاهی، دانشکده کشاورزی، دانشگاه لرستان

2 استاد، گروه اصلاح و بیوتکنولوژی گیاهی، دانشکده کشاورزی، دانشگاه ارومیه

3 استادیار، گروه تولیدات گیاهی، دانشکده کشاورزی، دانشگاه لرستان

4 استادیار، موسسه تحقیقات اصلاح و تهیه نهال و بذر کرج

چکیده

به‌منظور مطالعه تنوع ژنتیکی هندوانه بومی ایران از نظر صفات مورفولوژیک، 16 توده به همراه دو رقم هندوانه اصلاح شده (کریمسون‌سویت و چارلستون‌گری) در قالب طرح بلوک‌های کاملاً تصادفی با سه تکرار در مرکز تحقیقات کشاورزی ارومیه در سال 1392 مورد ارزیابی قرار گرفتند. تعداد 15 صفت شامل طول و عرض برگ، طول ساقه‌ی اصلی گیاه، تعداد میوه در بوته، طول میوه، عرض میوه، وزن کل میوه، وزن گوشت میوه، وزن پوست میوه، وزن صد بذر، طول بذر، عرض بذر، pH، درصد مواد جامد محلول و عملکرد مورد ارزیابی قرار گرفت. تجزیه واریانس نشان داد بین توده‌ها از لحاظ کل صفات مورد بررسی به جزء صفات pH، طول ساقه‌ی اصلی گیاه و تعداد میوه
در بوته اختلاف آماری معنی‌داری وجود دارد. متوسط عملکرد از 24926 کیلوگرم در هکتار برای ژنوتیپ چارلستون‌گری تا 13444 کیلوگرم در هکتار برای توده
817 همدانمتغییر بود. میزان درصد مواد جامد محلول در بین توده‌ها از 9/27 درصد برای ژنوتیپ کریمسون‌سویت تا 5/1 درصد برای توده‌ی 806 خراسان، pH میوه از 63/5 در توده‌ی 817 همدان تا 4/78 در ژنوتیپ چارلستون‌گری متغیر بود. بیشترین همبستگی مثبت (0/968) بین دو صفت وزن گوشت و وزن میوه و بیشترین همبستگی منفی (0/815-) بین صفات قند میوه و طول بذر مشاهده شد. بیشترین توارث‌پذیری (0/96) مربوط به وزن صد بذر و کمترین توارث‌پذیری (0/03) مربوط به pH بود. در تجزیه‌ی خوشه‌ای به روش وارد، توده‌های مورد بررسی در سه گروه متفاوت قرار گرفتند. بیشترین فاصله بین توده‌های گروه اول و سوم مشاهده شد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Exploring Genetic Diversity of Some Iranian Watermelon (Citrullus vulgaris) Accessions in Urmia Climatic Conditions

نویسندگان [English]

  • A. Hajiali 1
  • R. Darvishzadeh 2
  • B. Zahedi 3
  • J. Abbaskohpayegani 4
1 M.Sc. Student of Plant Production, Department of Plant Production, Faculty of Agriculture, Lorestan University, KorramAbad, Iran
2 Professor, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran
3 Assistant Professor, Department of Plant Production, Faculty of Agriculture, Lorestan University, KorramAbad, Iran
4 Assistant Professor of Seed and Plant Improvement Institute, Karaj, Iran
چکیده [English]

Background and Objectives
Watermelon (Citrullus lanatus Thunb) belongs to the genus Cucurbita and the family Cucurbitaceae. The aim of the present study was to evaluate the level of genetic variation among Iranian watermelon accessions originating from different regions of the country by morphological traits.
Materials and Methods
Sixteen accessions together with two commercial watermelon cultivars were planted in completely randomized block design with three replications in Agricultural Research Center of Urmia in 2013.Fifteen traits including leaf length, leaf width, plant length, number of fruit per plant, fruit length, fruit width, fruit weight, fruit mass, fruit skin, 100 seed weight, seed length, seed width, pH, TSS and yield were assessed on the studied accessions. During fruit ripening, four fruits from each plot were randomly selected and the traits were measured according to InternationalBoard for PlantGenetic Resources Institute descriptor. The TSS was assessed by using refractometer and the pH by using pH meter.
Results
Analysis of variance showed that there is a meaningful difference between genotypes for all studied traits except for pH, stem length and number of fruits per plant. According to the results, fruit yield ranged from 24626 kg h-1 in Charleston Gray genotype to 13444 kg h-1 in Hamedan 817 accession. The percentage of TSS varied from 9.27% in Crimson sweet genotype to 5.1% in Khorasan 806 accession and fruit pH varied from 5.63 in Hamedan 817 accession to 4.78 in Charleston Gray genotype. Phenotypic coefficient of variation was higher than genotypic coefficient of variation for all studied traits, indicating the significant effects of environmental effects. The highest positive correlation (0.968) was observed between pulp weight and fruit weight and the highest negative one (-0.815) was observed between TSS and seed length. The highest heritability was seen for 100 seed weight and the lowest one for pH. Cluster analysis with Ward method, classified the studied accessions in to three different groups. The maximum distance was observed between genotypes of groups 1 and 3.
Discussions
The results revealed a rather high level of genetic polymorphism and wide genetic variation between accessions, which offer valuable information for conservation and management of genetic resources and utilizing them in watermelon breeding programs. The genetic distance between accessions is a valuable parameter to conserve and use a given germplasm in breeding activities. It was proved that crosses between unrelated and genetically distant parents will show more power hybrid than crosses between genotypes closely related.

کلیدواژه‌ها [English]

  • Correlation
  • General heritability
  • Genetic diversity
  • Morphological traits
  • watermelon
References
Abd Mishani, S. and Shah Nejate Bushehri, A. (1997). Advanced plant breeding. Tehran University Press, Tehran, Iran, 320 P. [In Farsi]
Anonymous. (2013). Statistics yearbook of agricultural crops and animal science. Jihad-e- Keshavarzi Ministry Pub. [In Farsi]
Badenez, M.L., Martinez-Calvo, J., and Lacer, G. (1998). Analysis of apricot germplasm from the European ecogeographical group. Euphytica 102: 93-99.
Brown, W.L. (1983). Genetic diversity and genetic vulnerability-an appraisal. Economic Botany 37(1): 4-12.
Farahani, A. and Arzani, A. (2007). Study of genetic diversity of cultivars and F1 hybrids of durum wheat using agronomic and morphological traits. Journal of Science and Technology of Agriculture and Natural Resources 10: 341-355. [In Farsi]
Farshadfar, E. (1998). Application of biometrical genetics in plant breeding. Razi University Press. 528 P. [In Farsi]
Farsi, M. and Bagheri, A.R. (2004). Essentialls of plant breeding. Jihad Mashhad University Press. 375 P. [In Farsi]
Food and Agriculture Organization (FAO). (2012). Statistic Database. http/www.fao.org/ ag/agl/agll/spush. htm (accessed January 2015).
Kalloo, G. 1988. Vegetable Breeding. CRC Press, Florida, USA. 239 P.
Kiani, M. and Jahanbin, G. (2006). Investigation on genetic variation of Iran watermelon accession. Iranian Journal of Field Crops Research, 4(2): 333-345. [In Farsi]
Magss-Kolling, G.L. (2003). Variability in namibian landraces of watermelon (Citrullus lanatus). Euphytica 132(3): 251-258.
Mohammadi, R., Dehghani, H., Karimzadeh, Q., Fenny, D., and Akrami, M. (2014). Study on relationships between yield and its components in Iranian cantaloupe genotypes. Iranian Journal of Horticultural Sciences, 45(1): 1-10 [In Farsi]
Naroueirad M.R., Alah Dou M., Ghasemi A., and Fanaei H.R. (2010). Investigation of genetic diversity and broad sense heritability in watermelon accessions of Sistan. Iranian Journal of Horticultural Sciences (Iranian Journal of Agricultural Sciences), 40(4): 95-103.
Nevo, E., Noy-Meir, I., Beiles, A., Krugman, T., and Agami, M. (1991). Natural selection of allozyme polymorphisms: Micro-geographical spatial and temporal ecological differentiation in wild emmer wheat. Israel Journal of Botany 40: 419-449.
Peyvast, Gh. (2009). Vegetable crop production. Danshpazir Press, 5th Edition, Tehran, Iran. 577 P. [In Farsi]
Robinson, R.W. and Decker-Walters, D.S. (1997). Cucurbits. CAB international. Wallingford, UK. 226 P.
Romao, R.L. and Roberto, L. (2000). Notheast Brazil: A secondry center of diversity for watermelon (Citrullus lanatus). Genetic Resources and Crop Evaluation, 47(2): 207-213.
Sheikh, S., Noh, J., Seong, M., Jung, G., Kim, J., Ju, H., and Huh, Y. (2013). Phenotypic markers for tetraploid watermelon (Citrullus lanatus (Thunb.) following parental exposure to colchicine in to generation. Horticulture, Environment, and Biotechnology 54(6): 524-530.
Shekari, F., Massiha, S., and Esmailpoor, B. (2006). The physiology of vegetable crops. Zanjan University Press, 394 P. [In Farsi]
Stansfield, W.D. (1991). Theory and problems in genetics. McGraw-Hill.
Szamosi, C., Solmaz, I., Sari, N. and Barsony, C.( 2009). Morphogical characterization of Hungarian and Turkish watermelon (Citrullus lanatus (Thunb.) Matsum. et Nakai) genetic resources. Genetic Resources and Crop Evaluation 56: 1091-1105.
Wehner, T.C. (2000). Watermelon crop information. Department of Horticulture Science, North Carolina State University.