Document Type : Research Paper




Background and Objectives
Grapes are generally grown in different regions of Iran. Quality of table grapes is usually considered as a combination of appearance and flavor during shelf-life. Brassinosteroids are considered to be a class of plant polyhydroxysteroids and have been recognized as a new kind of phytohormones that play an essential role in plant development. Researchers studied the brassinosteroids levels in cucumber through a chemical genetics approach and found that BR levels were positively correlated with the tolerance to cold stresses. The objectives of this study were to evaluate the effects of BR on chilling d, lipid peroxidation content, and the induction of antioxidant enzymes, such as ascorbate peroxidase and superoxide dismutasein “Rish Baba” grape fruit during storage at 0-1 °C.
Materials and methods
Grape fruits were harvested and transported to the laboratory on the same day. The harvested fruits were washed, and dried in air. Grape fruits were treated with 0 (control), 0.75 and 1.5 milligram per liter brassinosteroid for 5 min and then stored at 0-1°C, 85-90 % relative humidity for 6 weeks. Characteristics such as chilling injury, ion leakage, lipid peroxidation, and activity of antioxidant enzymes were evaluated.
Results showed that brassinosteroid significantly reduced the chilling injury, ion leakage, lipid peroxidation and hydrogen peroxide, in treated fruits, compared to control. According to these results, pH and ascorbic acid of treated and controlled fruits increased during storage while titratable acidity of fruits decreased. As for brassinosteroid treatment, the pattern of mentioned changes reduced lower than control. So, the activity of antioxidant enzymes of fruits treated with brassinosteroid highly increased compared to control during storage. Fruits treated with 1.5 mgL-1 brassinosteroid showed the best effect with lowest chilling injury.
In the present study, the plant hormone BR was applied and the results indicated that BR significantly reduces CI of grape fruits during storage at 0-1 °C. Our finding was consistent with previous reports that exogenous application of BR is effective in protecting seedlings of rice, maize and cucumber against cold stress. CI occurrence is often accompanied by oxidative damage, which can be followed through lipid peroxidation content, since it is a final product of lipid peroxidation. In this study, there was a continuous increase in fresh tissue lipid peroxidation content in all fruits, but the application of BR significantly delayed the increase of lipid peroxidation. Moreover, the change in membrane permeability showed trends similar to lipid peroxidation content; that is, fresh tissue H2O2 content increased with storage duration, but BR markedly delayed the increase. BR has been considered to be involved in a network of interacting signal transduction pathways, which regulate defense responses to abiotic stress. When horticultural crops are exposed to severe abiotic stresses, including cold stress, large amounts of intracellular ROS are generated. The detoxification of ROS is dependent on antioxidant enzymes such as superoxide dismutase. The increase in these enzymes’ activity contributes to the adaptation of plants to cold stress and ameliorates oxidative damage such as lipid peroxidation and H2O2 content.


Main Subjects

  1. Abdollahi, R., Dowlati Baneh, H., and Masoomi, A. Effect of nitric oxide on Askari grape during storage. Journal of Small Fruits, 2: 1-14. [In Farsi]
  2. Aghdam, M.S., Asghari, M., Farmani, B., Mohayeji, M., and Moradbeygi, H. 2012. Impact of postharvest brassinosteroids treatment on PAL activity in tomato fruit in response to chilling stress. Scientia Horticulturae, 144: 116-120.
  3. Asada, K. 1999. The water-water cycle in chloroplast: Scavenging of active oxygen and dissipation of excess photons. Annual Review of Plant Physiology and Plant Molecular Biology, 50: 601-639.
  4. Burdurlu, H.S., Nuray, K., and Feryal, K. 2006. Degradation of vitamin C in citrus juice concentrates during storage. Journal of Food Engineering, 74: 211-216.
  5. Cioroi, M. 2007. Study on L-ascorbic acid contents from exotic fruits. Cercetari Agronomic in Moldova, 1: 23-27.
  6. Clous, S.D. and Sasse, M. 1998. Brassinosteroids: essential regulators of plant

growth and development. Annual Physiology Reviews, 49: 427-451.

  1. Considine, J.A. and Kriedemann, P.E. 1972. Fruit splitting in grapes: Determination of the critical turgor pressure. Australian Journal of Agricultural Research, 23: 17-24.
  2. Forney, C.F. and Peterson, S.J. 1990. Chilling induced potassium leakage of cultured Citrus cells. Physiology Plant, 78: 193-196.
  3. Giannopolitis, C.N. and Ries, S.K. 1977. Superoxide dismutase. I. Occurrence in higher plants. Plant Physiology, 59: 309-314.
  4. Heath, R.L. and Packer, L. 1969. Photoperoxidationin isolated chloroplast, kinetics and stoichiometry of fatty acid peroxidation. Archive of Biochemistry and Biophysiology, 125: 189-198.
  5. Kelebek, H., Selli, S., Canbas, A., and Cabaroglu, T. 2009. HPLC determination of organic acids, sugars, phenolic composition and antioxidant capacity of orange wine made from a Turkish cv. Kozan. Microchemical Journal, 91: 187-192.
  6. Kim, T.W. and Wang, Z.Y. 2010. Brassinosteroid signal transduction from receptor kinases to transcription factors. Annu Rev Plant Biol, 61: 681-704.
  7. Khripach, V. and Zhabinskii, V. 1998. Brassinosteroids: A new class of plant hormones acadamic press. United States of Amenca, 460
  8. Montesinos-Herrero, C. and Palou, L .2010. Combination of physical and low-toxicity chemical postharvest treatments for the management of citrus fruit: A review. Stewart Postharvest Review, 54: 72-79.
  9. Mosymons, G.M., Davies, C., Shavrukov, Y., Bodry, I., Reid, J.B., and Thomas, M. 2006. Graps on steroids. Brassinosteroids are involved in grape berry ripening. Plant physiology, 140: 150-158.
  10. Nakano, Y. and Asada, K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach choloroplast. Plant Cell Physiol, 22: 867-880.
  11. Nilprapruck, P., Authanithee, , and Keebjan, P. 2008. Efeect of exogenous methyljasmonate on chilling injury and quality of pineapple. Silpakorn University Science and Techology, 2: 33-42.
  12. Odriozola-Serrano, I., Hernndez-Jover, T., and Martn-Belloso, O. 2007. Comparative evaluation of UV-HPLC methods and reducing agents to determine vitamin C in fruits. Food Chem, 105: 1151-1158.

19.    Peng, J., Tang, X., and Feng, H. 2004. Effects of brassinolide on the physiological properties of litchi pericarp (Litchi chinensis L.). Science Horticulturae, 101: 407-416.

  1. Pignocchi, C.C. and Foyer, H. 2003. Apoplastic ascorbate metabolism and its role in the regulation of cell signaling. Current Opinion in Plant Biology, 6: 379-389.
  2. Pipattanawong, N., Fujishige, N., Yamane, K., and Ogata, R. 1996. Effect of brassinosteroidon vegetative and reproductive growth in two day-neutral strawberries. Horticultural Science, 65: 651-654.
  3. Sairam, R.K., Deshmukh, P.S., and Shukla, D.S. 1997. Tolerance to drought and temperature stress is relation to increased antioxidant enzyme activity in wheat. Journal of Agronomy Crop Science, 178: 171-177.
  4. Sha, S.F., Li, J.C., and Zhang, S.L. 2011. Change in the organic acid content and releted metabolic enzyme activities in developing xinping pear fruit. African Journal of Agricultural Research, 6: 3560-3566.
  5. Schirra, M. and D’hallewin, G. 1997. Storage performance of Fortune mandarins following hot water dips. Postharvest Biology and Technology, 10: 229-238.
  6. Schirra, M., Mulas, M., Fadda, A., Mignani, I., and Lurie, S.2005. Chemical and quality traits of ‘Olinda’ and ‘Campbell’ oranges after heat treatment at 44 or 46 C for fruit fly disinfestations. Lebenson. Wiss. Plant Technology, 38: 519-527.
  7. Shahbaz, M. and Ashraf, M. 2007. Influence of exogenous application of brassinosteroid on growth and mineral nutrients of wheat under saline conditions. Pak, 39(2): 513-522.
  8. Velikova, V., Yordanov, I., and Edreva, A. 2000. Oxidaive stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Science, 151: 59-66.
  9. Wang, C.Y. 1990. Alleviation of chilling injury of horticultural crops. In: C.Y. Wang (Ed), Chilling injury of horticultural crops. CRC Press, Boca Raton, FL., pp: 281-320.
  10. Zokaee-Khosroshahi, M.R. and Esna-Ashari, M. 2007. Postharvest putresciene treatments extend the storage-life of apricot (Prunus armeniaca) etokhm sefid, fruit. Journal of Horticultural Science and Biotechnology, 82: 986-990.
  11. Zokae-khosroshahi, M.R., Esna-Ashariand, M., and Ershadi, A. 2007. Effect of exogenous puterscine on post-harvest life of strawberry (Fragari ananassa Duch) fruit, cultivare selva. Scientia Horticulturae, 114: 27-32.
  12. Zhang, A., Zhang, J., Ye, N., Cao, J., Tan, M., Zhang, J., and Jiang, M. 2010. ZmMPK5 is required for the NADPH oxidase-mediated self-propagation of apoplectic H2O2 in brassinosteroid-induced antioxidant defence in leaves of maize. Journal of Experimental Botany, 61: 4399-4411.
  13. Zhu, Z., Zhang, Z., Qin, G., and Tian, S.P. 2010. Effects of brassinosteroids on post-
    harvest disease and senescence of jujube fruit in storage. Postharvest Biology and Technology, 56: 50-55.