Document Type : Research Paper



Background and Objectives
Environmental pollution is the result of industrial societies and industrialization of human society. Copper is an essential micronutrient for plants growth and development when the copper concentration in the water is high, as one of the most toxic heavy metals for living organisms. Some plants are known as copper accumulator. Watercress (Nasturtium officinale) is an aquatic plant species from Brassicaceae family. Greenhouse experiments have showed that watercress has high accumulation ability of some heavy metals.
Material and methods
This study aimed to examine the effects of copper on growth and physiological characteristics of Nasturtium officinale. Accordingly, effects of different levels of copper sulfate (0, 4, 8, 12, 16 µM)
in N. officinale in a complete randomized design with three replications were studied. Rooted cuttings of N. officinale were transferred to hydroponic culture, in 1-L polyethylene pots containing a modified half-strength Hoagland’s solution. Nutrient solutions were renewed weekly and plants were grown in a growth chamber (20/15 °C day/night; light intensity 200 µE m-2 s-1, 16 h day-1; relative humidity 75 %). After 14 days of pre-culture, plants were exposed to different levels of copper sulfate. The plants were harvested for analysis after having grown in the test solution for 2 weeks.
The results showed that in the 4 µM copper treatment, fresh and dry weight of shoots, shoot length, leaf area and RWC increased, but at higher concentrations (8, 12, 16 µM) these characteristics significantly decreased. The root and shoot copper concentrations consistently increased with increasing copper exposure. At the 16 µM of copper in the nutrient solution, the average of copper concentrations in root and shoot was 4210 and 558 µg/g d.w., respectively. According to the results, shoot copper concentration was lower than the root and very little of that was transferred into the shoot.
In total, according to the results, copper sulfate significantly decreases the shoots and roots of fresh and dry weight as well as root length, shoot length and leaf area, and increases the total chlorophyll, but no significant effect on the amount of Protein, Carbohydrate, Anthocyanin and Carotenoid. Generally, the threshold of this plant is to 4 µM copper.


Main Subjects

  1. Ali, A.A. and Alqurainy, F. 2006. Activities of antioxidants in plants underenvironmental In: Motohashi, N. The lutein-prevention and treatment for diseases. Transworld Research Network, India, pp: 187-256.
  2. Archambault, C.J. and Winterhalder, K. 1995. Metal tolerance in Agrostis scabra from the Sudbury Ontario area. Canadian Journal of Botany, 73: 766-775.
  3. Arnon, D.I. 1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in beta vulgaris. Plant Physiology, 24: 1-15.
  4. Baker, A.J.M. and Walker, P.L. 1990. Ecophysiology of metal uptake by tolerant plants. In: Shaw, A.J. Heavy metal tolerance in plants: evolutionary aspects. CRC Press, Boca Raton, Florida, pp: 155-177.
  5. Chaffai, R., Tekitek, A., and El-Ferjani, E. 2005. Comparative Effects of copper and cadmium on Growth and Lipd content in maize seedlings (Zea mays ). Pakistan Journal of Biological Sciences, 8 (4): 649-655.
  6. Chapin, M.F. and Kennedy, G.F. 1987. Carbohydrate analysis. Lloydia, 22: 111-115.
  7. Faust, M.B. and Christians, N.E. 2000. Copper reduces shoot growth and root development of Creeping Bentgrass. Crop Science, 40(2): 498-502.
  8. Ghasemi, R., Ghaderian, S.M., and Kramer, U. 2009. Interference of nickel with copper and iron homeostasis contributes to metal toxicity symptoms in the nickel hyperaccumulator plant Alyssum inflatum. New Phytologist, 184: 566-580.
  9. Ghorbanli, M. and Kiapour, A. 2012. Copper-induced changes on pigments and activity of non-enzimatic and enzymatic defence systems in Portulaca oleracea Iranian Journal of Medicinal and Aromatic Plants, 28(2): 235-247. [In Farsi]

10.    Hall, J.L. 2002. Cellular mechanisms for heavy metal detoxification and tolerance. Journal of Experimental Botany, 53(366): 1-11.

11.    Hosseinzadeh, P., Mohtadi, A., Movahedi Dehnavi, M., and Asmaneh, T. 2016. Effect of different zinc levels on some physiological characteristics of Plantago ovata under salt stress. Journal of Plant Process and Function, 5(15): 157-168. [In Farsi]

  1. Khaveri Nejad, R.A., Najafi, F., and Babri Bonab, R. 2010. Effects of different concentrations of copper sulfate (CuSO4) on certain physiological parameters of Bean (Phaseolus vulgaris). Quarterly Journal of biological Science, 4(11): 77-85. [In Farsi]
  2. Laspina, N.V., Groppa, M.D., and Benavides, M.P. 2005. Nitric oxide protects sunflower leaves against Cd-induced oxidative stress. Plant Science, 169(2): 323-330.
  3. Lichtenthaler, K. 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods in enzymology, 148: 350-382.
  4. Liu, D., Li, T.Q., Jin, X.F., Yang, X.E., Islam, E., and Mahmood. Q. 2008. Lead induced changes in the growth and antioxidant metabolism of the lead accumulating and non-accumulating ecotypes of Sedum Alfredii. Journal of Integrative Plant Biology, 50(2): 129-140.
  5. Luna, C.M., Gonzalez, C.A., and Trippi, V.S. 1994. Oxidative damage caused by an excess of copper in oat leaves. Plant and Cell Physiology, 35: 11-15.
  6. Marschner, H. 2012. Mineral nutrition of higher plants. Third edition. Academic Press, London, UK.
  7. Monni, S., Salemaa, M., and Millar, N. 2000. The tolerance of Empetrum nigrum to copper and nickel. Environmental Pollution, 109(2): 221-229.
  8. Ouzounidou, G., Ciamporova, M., Moustakas, , and Karataglis, S. 1995. Responses of maize (Zea mays L.) plants to copper stress I. Growth, mineral content and ultrastructure of roots. Environmental and Experimental Botany, 35: 167-176.
  9. Rascio, N. and Navari-Izzo, F. 2011. Heavy metal hyperaccumulating plants: How and why do they do it? And what makes them so interesting? Plant Science, 180: 169-181.
  10. Robinson, B., Duwing, C., Bolan, N., Kannathasan, M., and Saravanan, A. 2003. Uptake of arsenic by New Zealand watercress (Lepidium sativum ). Science Total Environment, 301: 67-73.
  11. Schat, H. and Ten Bookum, W.M. 1992. Genetic control of copper tolerance in Silene vulgaris. Heredity, 68: 219-229.
  12. Serida, K., Mohammad, B.A., Eun, J.H., and Kee, Y.P. 2008. Copper toxicity in Withania somnifera: Growth and antioxidant enzymes responses of in vitro grown plants. Environmental and Experimental Botany, 64: 279-285.
  13. Sheldon, A. and Menzies, N.W. 2005. The effect of copper toxicity on the growth and morphology of Rhodes grass (Chloris gayana) in solution culture. Plant and Soil, 278(1-2): 341-349.
  14. Shulan, Z., Qing L., Yanting, Q., and Lian, D. 2010. Responses of root growth and protective enzymes to copper stress in turf grass. Acta Biologica Cracoviensia Series Botanica, 52(2): 7-11.
  15. Sosse, B.A., Genet, P., Dunand, F.V., Toussaint, M.L., Epron, D., and Badot, P.M. 2004. Effect of copper on growth in cucumber plants (Cucumis sativus) and its relationships with carbohydrate accumulation and changes in ion contents. Plant
    Science, 166: 1213-1218.
  16. Wang, Y.S. and Yang, Z.M. 2005. Nitric oxide reduces Aluminum toxicity by preventing oxidative stress in the roots of Cassia tora Plant Cell Physiology, 46(12): 1915-1923.
  17. Wanger, G.J. 1979. Content and vacuole extra vacuole distribution of neutral sugars, free amino acids, and anthocyanins in protoplast. Plant Physiology, 64: 88-93.
  18. Yalcin, M.G., Battaloglu, R., and Ilhan, S. 2007. Heavy metal sources in Sultan Marsh and its neighborhood, Kayseri, Turkey. Environmental Geology, 53(2): 399-415.
  19. Yamasaki, S. and Dillenburg, L.C. 1999. Measurements of leaf relative water content in Araucaria angustifolia. Revista Brasilleira de Fisiologia Vegetal, 11(5): 69-75.
  20. Yruela, I. 2005. Copper in plants. Brazilian Journal of Plant Physiology, 17(1):