Document Type : Research Paper
Authors
Abstract
Introduction: Micropropagation is the best approach to propagation of Fritillaria and prevention of its extinguishing. The effects of auxin and cytokinin combinations on callus and bulblet production using explants taken from daughter bulb scale of two fritillary clones collected from Arjan and Koohrang plains of Fars province were studied.
Materials and methods: For this purpose, 3 types of growth regulator combinations containing three levels of NAA (0, 0.5 and 1 mg L-1) with one of the cytokines (2ip, Kinetin and TDZ) at three levels (0.5, 1.0 and 2 mg L-1) were applied. The base medium was MS containing 4.5 percent sucrose and 8 g/l agar. To evaluate the effects of these factors, experimental design of completely randomized factorial with 4 replications and 5 explants per repeat was used.
Background and Objectives
Micropropagation is the best approach to propagation of Fritillaria and prevention of its extinction. The effects of auxin and cytokinin combinations on callus and bulbletproduction using explants taken from daughterbulb scale of two fritillary clones collected from Arjan and Koohrang plains of Fars province were studied.
Materials and methods
For this purpose, 3 types of growth regulator combinations containing three levels of NAA (0, 0.5 and 1.0 mg L-1) with one of the cytokines (2ip, Kinetin and TDZ) at three levels (0.5, 1.0 and 2.0 mg L-1) were applied. The base medium was MS containing 4.5 percent sucrose and 8 g/l agar. To evaluate the effects of these factors, an experimental design of completely randomized factorial with 4 replications and 5 explants per repeat was used.
Results
Analysis of data revealed that the kinetin and 2ip had no significant effect on callus induction percentage while TDZ was significant on this parameter. The highest callus production was observed in medium containing 0.5 mg L-1 NAA combined with 0.5 mg L-1 TDZ. Using this treatment, 60 percent of explants produced the callus. The most number of direct and indirect regenerated bulblets were recorded in medium complemented with 1.0 mg/l NAA and 1.0 mg L-1 Kinetin. The mean of 5.5 bulbletper explants was obtained in this treatment.
Discussion
Considering the effectiveness and cost of NAA and environmental issues, its lower concentration is recommended for bulblet regeneration in this plant.
Keywords
Main Subjects
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