Document Type : Research Paper

Authors

1 Msc Student in Seed Science and Technology, Shahrekord University, Shahrekord

2 Assistant Professor, Department of Horticulture, Shahrekord University, Shahrekord

Abstract

Background and Objectives
Dragonhead or Moldavian dragonhead (Dracocephalum moldavica L.), which belong to Lamiaceae family is an annual medicinal herbaceous and aromatic plant that its essential oil is used widely in medicine, food, cosmetic and health industries. Due to the importance and role of medicinal plants in various industries, there is an increasing demand for herbal medicines. The indiscriminate use of chemical fertilizers has increased crop production, but has also harmed ecosystems. Some studies show that the use of some microbial species that are collectively designated as plant growth promoting rhizobacteria (PGPR) can improve yield and quality of medicinal plants. Given the importance of environmental health and food safety, this study aimed to investigate the effect of PGPR on morphological traits and essential oil content of Moldavian dragonhead.
Materials and methods
This experiment was conducted in randomized complete block design with eight treatments and three replications at research station of faculty of agriculture, Shahrekord university. No bacterial inoculation was used as control and seeds inoculated with Rhodococcus sp., Corynebacterium sp., Mycobacterium sp., Bacillus sp., Azotobacter sp., Pseudomonas putida, Pseudomonas fluorescens were considered as bacterial treatments.
Results
The results showed that all bacterial treatments had a significant effect on plant height, number of branches, number of flowering shoot, leaf dry weight, shoot dry weight, biological yield, crop growth rate (CGR), leaf area (LA), chlorophyll a, chlorophyll b, essential oil content and yield. Mycobacterium sp. treatment had the most effect on plant height, number of branches, leaf and shoot dry weight, number of flowering shoot, biomass and chlorophyll b. Bacillus sp. treatment had the most effect on leaf dry weight, number of flowering shoot, leaf area and essential oil content and yield. The highest crop growth rate and chlorophyll a were observed in Rhodococcus sp.treatment.
Discussion
In general, the Bacillus sp., Mycobacteriumsp., Rhodococcus sp., andPseudomonas putida treatments had the most influence on Moldavian dragonhead. Since the purpose of medicinal production is obtaining the highest content of essential oil and biomass yield, based on these results, the use of Bacillus sp., and Mycobacterium sp., bacterial inoculation treatments, as biopriming, can be recommended for increasing biomass and essential oil content in Moldavian dragonhead.
 

Keywords

Main Subjects

  1. Abbas-zadeh, P., Savaghebi, G.R., Asadi-Rahmani, H., Rejali, F., Farahbakhsh, M. Motesharezadeh, B., and Omidvari, M. 2012. The effect of fluorescent pseudomonas on increasing the solubility of zinc compounds and improve its absorption by bean (Phaseolus vulgaris). Iranian Journal of Soil Research, 26(2): 197-206. [In Farsi]
  2. Banchio, E., Bojino, P., Zygadlo, J., and Giordano, W. 2008. Plant growth promoting rhizobacteria improve growth and essential oil yield in Origanum majorana Biochemical Systematics and Ecology, 36:766-771.
  3. Burd, G.I., Dixon, D.G., and Glick, B.R. 1998. A plant growth-promoting bacterium that decreases nickel toxicity in seedlings. Journal of Applied and Environmental Microbiology, 64: 3663-366.
  4. El-Ghadbane, E.A.E., Shalan, M.N., and Abdel-Latife, T.A.T. 2006. Influence of biofertilizers on growth, volatile oil yield and constituents of fennel (Foeniculum vulgar Mill.). Egyptian Journal of Agricultural Research, 84(3): 977-992.
  5. Erturk, Y., Cakmakci, R., Duyar, O., and Turan, M. 2011. The effects of plant growth promoting rhizibacteria on vegetative growth and leaf nutrient contents of hazelnut seedling (Turkish hazelnut CV. Tombul and Sivir). International Journal of Soil Science, 6 (3): 188-198.
  6. Han, H., Supanjan, S., and Lee, K.D. 2006. Effect of co-inoculation with phosphate and potassium solubilizing bacteria on mineral uptake and growth of pepper and cucumber. Plant Soil Environ, 52(3): 130-136.
  7. Jahanshahi, Sh., Baghreizadeh, M., and Abotalebi, A. 2013. Effect of vermi compost, azotobacter and barvar II on some quantitative and qualitative traits of coriander (Coriandrum sativum) medicinal plant. Journal of Crop Production Research, 4(4): 391-400. [In Farsi]
  8. Jamshidi, A., Gholavand, A., Sefidkon, F., and Mohamadi Gholtape, A. 2012. The effect of nutrition systems on quantitative and qualitative characteristics of Fennel (Foeniculum vulgare Mill.) under water deficit stress. Iranian Journal of Medical and Aromatic Plants, 28(2): 309-323. [In Farsi]
  9. Kandeel, A.M., Naglaa, S.A.T., and Sadek, A. 2002. Effect of biofertilizers on the growth, volatile oil yield and chemical composition of Ocimum basilicum Annals of Agricultural Science, 47: 351- 371.
  10. Kaymak, H.C., Yarali, F., Guvence, I., and Donmeze, M.F. 2008. The effect of inoculation with plant growth promoting rhizobacteria (PGPR) on root formation of mint (Mentha piperita) cuttings. African Journal of Biotchnology, 7(24): 4479-4483.
  11. Kazemalilou, S. and Rasouli-Sedghiani, M.H. 2012. Effect of soil cadmium pollution on some physiological parameters of Hyoscyamus plant (Hyoscyamus niger) in presence/absence of growth-promoting microorganisms. Journal of Water and Soil Science, 22(4): 17-30. [In Farsi]
  12. Leithy, S., El-Meseiry, T.A., and Abdallah, E.F. 2006. Effect of biofertilizers, cell stabilizer and irrigation regime on rosemary oil yield and quality. Journal of Applied Sciences Research, 2(10): 773-779.
  13. Mafakheri, S., Omidbaigi, R., Sefidkon, F., and Rejali, F. 2012. Effect of vermicompost, biophosphate and azotobacter on quantity and quality of essential oil of Dracocephalum moldavica L. Iranian Journal of Medicinal and Aromatic Plants, 27(4): 245-254. [In Farsi]
  14. Naderi, M.R. 2012. The effect of plant growth promoting rhizobacteria on phytoremediation of Lead by sunflower in a Pb-bearing soil for long term. M.Sc. Thesis in Agroecology, Shahrekord University, 119 pp. [In Farsi]
  15. Rajaei, S., Raeisi, F., and Alikhani, H.A. 2007. Effect of growth promoters potential of native strains of Azotobacter chroococcum on growth, yield and nutrient uptake in wheat. Scientific Journal of Agriculture, 30 (4): 33-47. [In Farsi]
  16. Rodriguez, H. and Fraga, R. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances, 17: 319-339.
  17. Saghafi, D., Alikhani, H.R., and Motesharezadeh, B. 2013. Effect of plant growth promoting rhizobia on improving the nutritional status of canola (Brassica napus) under salinity stress. Journal of Water and Soil Science, 23(4): 159-176. [In Farsi]
  18. Sarcheshmeh poor, M., savaghebi, G.R., Siadat, H., and Alikhani, H. A. 2014. The effect of plant growth rhizobacteria on improvement of growth and nutrition of pistachio seedlings under drought stress. Iranian Journal of Soil Research, 27(1): 107-119. [In Farsi]
  19. Sheikhi-Ghahfarokhi, F. 2014. Effect of seed biopriming by PGPR bacteria on germination indices, growth and yield of Calendula of ficinalis L. M.Sc. Thesis in Seed Science and Technology, Shahrekord University, 93 pp. [In Farsi]
  20. Taiz, L. and Zeiger, E. 2002. Plant Physiology. 3rd Sinaure Sunderland, 690 pp.
  21. Tsavkelova, E.A., Cherdyntseva, T.A., and Netrusoe, A.I. 2005. Auxin production by bacteria associated with orchod roots. Microbiology, 74: 233-273.
  22. Vital, W.M., Teixeira, N.T., Shigihara, R., and Dias, A.F.M. 2002. Organic manuring with pig biosolids with application of foliar biofertilizers in the cultivation of thyme (Thymus vulgaris). Ecossistema, 27: 69-70.
  23. Wellburn, A.R. 1994. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Journal of Plant Physiology, 144: 307-313.
  24. Yousefzadeh, S., Modarres-Sanavy, S.A.M., Sefidkon, F., Asgarzadeh, A., Ghalavand, A., Roshdi, M., and Safaralizadeh, A. 2013. Effect of biofertilizer, azocompost and nitrogen on morphologic traits and essential oil content of Dracocephalum moldavica L. in two regions of Iran. Iranian Journal of Medicinal and Aromatic Plants, 29 (2): 438-459. [In Farsi]
  25. Youssef, A.A., Edris, A.E., and Gomaa, A.M. 2004. A comparative Study between some plant growth regulators and certain growth hormones producing microorganisms on growth and essential oil composition of Salvia of ficinalis L. Plant Annals of Agricultural Science, 49: 299-311.