نوع مقاله : علمی - پژوهشی
نویسندگان
1 دانشجوی کارشناسی ارشد رشته علوم و تکنولوژی بذر، دانشگاه شهرکرد
2 استادیار، گروه مهندسی زراعت، دانشکده کشاورزی، دانشگاه شهرکرد
3 استادیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه شهرکرد
چکیده
به‌منظور بررسی تأثیر تیمار بیولوژیک بذر با باکتری‌های محرک رشد گیاه بر شاخص‌های مرفولوژیک و میزان اسانس گیاه دارویی بادرشبو، آزمایشی به صورت بلوک‌های کامل تصادفی با 8 تیمار و 3 تکرار در مزرعه تحقیقاتی دانشکده کشاورزی دانشگاه شهرکرد اجرا گردید. عدم تلقیح باکتریایی به‌عنوان تیمار شاهد و تلقیح جداگانه بذور با باکتری‌های محرک رشد شامل Rhodococcus sp.، Corynebacterium sp.، Mycobacterium sp.، Bacillus sp.،Azotobacter sp. ، Pseudomonas putida، Pseudomonas fluorescensبه‌عنوان تیمارهای باکتریایی در نظر گرفته شدند. نتایج این مطالعه نشان داد که تیمارهای تلقیح باکتریایی بر ارتفاع بوته، تعداد شاخه فرعی، تعداد سرشاخه گلدار، وزن خشک برگ، وزن خشک ساقه، عملکرد بیولوژیک، سرعت رشد گیاه، سطح برگ، کلروفیل a، کلروفیل b، درصد اسانس و عملکرد اسانس اثر معنی‌داری داشتند. تیمار تلقیح باکتریایی Mycobacterium sp. بیشترین تأثیر را بر ارتفاع بوته، تعداد شاخه‌های فرعی، وزن خشک برگ و ساقه، تعداد سرشاخه گلدار، عملکرد بیولوژیک وکلروفیل b داشت. تیمار تلقیح باکتریایی Bacillus sp. بیشترین تأثیر را بر وزن خشک برگ، تعداد سرشاخه گلدار، سطح برگ، درصد و عملکرد اسانس داشت. بیشترین سرعت رشد گیاه و هم‌چنین بالاترین میزان کلروفیل a در تیمار تلقیح باکتریایی Rhodococcus sp. مشاهده گردید. در مجموع تیمارهای Bacillus sp.، Mycobacterium sp.، Rhodococcus sp. و Pseudomonas putida بیشترین تأثیر را بر صفات مورد بررسی داشتند. از آنجا که هدف از تولید اغلب گیاهان دارویی استحصال بالاترین درصد اسانس و عملکرد بیولوژیک می‌باشد، با توجه به نتایج این پژوهش، در مجموع استفاده از تیمارهای تلقیح باکتریایی Bacillus sp. و Mycobacterium sp. به صورت بیوپرایمینگ جهت افزایش عملکرد کمی و کیفی گیاه دارویی بادرشبو توصیه می‌گردد.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
Effect of Plant Growth Promoting Rhizobacteria on Morphological Traits and Essential oil Content of Moldavian Dragonhead (Dracocephalum moldavica L.)
نویسندگان [English]
- Vafa Torfi 1
- A D 2
- Keramatollah Saeidi 3
1 Msc Student in Seed Science and Technology, Shahrekord University, Shahrekord
3 Assistant Professor, Department of Horticulture, Shahrekord University, Shahrekord
چکیده [English]
Background and Objectives
Dragonhead or Moldavian dragonhead (Dracocephalum moldavica L.), which belong to Lamiaceae family is an annual medicinal herbaceous and aromatic plant that its essential oil is used widely in medicine, food, cosmetic and health industries. Due to the importance and role of medicinal plants in various industries, there is an increasing demand for herbal medicines. The indiscriminate use of chemical fertilizers has increased crop production, but has also harmed ecosystems. Some studies show that the use of some microbial species that are collectively designated as plant growth promoting rhizobacteria (PGPR) can improve yield and quality of medicinal plants. Given the importance of environmental health and food safety, this study aimed to investigate the effect of PGPR on morphological traits and essential oil content of Moldavian dragonhead.
Materials and methods
This experiment was conducted in randomized complete block design with eight treatments and three replications at research station of faculty of agriculture, Shahrekord university. No bacterial inoculation was used as control and seeds inoculated with Rhodococcus sp., Corynebacterium sp., Mycobacterium sp., Bacillus sp., Azotobacter sp., Pseudomonas putida, Pseudomonas fluorescens were considered as bacterial treatments.
Results
The results showed that all bacterial treatments had a significant effect on plant height, number of branches, number of flowering shoot, leaf dry weight, shoot dry weight, biological yield, crop growth rate (CGR), leaf area (LA), chlorophyll a, chlorophyll b, essential oil content and yield. Mycobacterium sp. treatment had the most effect on plant height, number of branches, leaf and shoot dry weight, number of flowering shoot, biomass and chlorophyll b. Bacillus sp. treatment had the most effect on leaf dry weight, number of flowering shoot, leaf area and essential oil content and yield. The highest crop growth rate and chlorophyll a were observed in Rhodococcus sp.treatment.
Discussion
In general, the Bacillus sp., Mycobacteriumsp., Rhodococcus sp., andPseudomonas putida treatments had the most influence on Moldavian dragonhead. Since the purpose of medicinal production is obtaining the highest content of essential oil and biomass yield, based on these results, the use of Bacillus sp., and Mycobacterium sp., bacterial inoculation treatments, as biopriming, can be recommended for increasing biomass and essential oil content in Moldavian dragonhead.
کلیدواژه‌ها [English]
- Dracocephalum moldavica
- Biopriming
- Biological fertilizer
- Crop growth rate
- Chlorophyll
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