نوع مقاله : علمی - پژوهشی
نویسندگان
1 استادیار، گروه زراعت و اصلاح نباتات دانشکده علوم کشاورزی دانشگاه گیلان
2 دانشجوی دکتری زراعت دانشکده علوم کشاورزی دانشگاه گیلان
چکیده
به‌منظور بررسی تأثیر باکتری‌های محرک رشد و سطوح مختلف کود شیمیایی فسفر بر عملکرد و اجزاء عملکرد دانه ذرت هیبرید 540 تحت رژیم‌های مختلف رطوبتی، آزمایشی به صورت کرت‌های نواری در قالب طرح پایه بلوک‌های کامل تصادفی با سه تکرار در مرکز آموزش کشاورزی شهید ناصری کرج در دو سال زراعی 92-1391 و 93-1392 اجرا شد. با اجرای سیستم آبیاری تک شاخه 4 سطح آبیاری (بدون تنش، تنش ملایم، تنش متوسط، تنش شدید) اعمال شد. عامل فسفر نیز در 5 سطح شامل 100 درصد کود شیمیایی سوپرفسفات‌تریپل و بدون کود زیستی، 75 درصد کود شیمیایی همراه با کود زیستی،50 درصد کود شیمیایی همراه با کود زیستی، 25 درصد کود شیمیایی همراه با کود زیستی، کود زیستی و بدون کود شیمیایی بود. نتایج آزمایش حاکی از معنی‌دار بودن تیمار کودی در تمامی صفات مورد مطالعه در سال اول و دوم بود. در مقایسه اثر سطوح مختلف کود فسفر مشخص گردید که در شرایط بدون تنش، تیمار بدون تلقیح با باکتری و 100 درصد کود فسفر مورد نیاز، بالاترین میزان عملکرد دانه را نشان داد، در‌حالی‌که با افزایش میزان تنش، میزان عملکرد دانه برای تیمار تلقیح با باکتری و 75 درصد کود فسفر مورد نیاز به‌ترتیب در شرایط تنش کم، تنش متوسط و تنش شدید به میزان 2/1 درصد، 5/5 درصد و 35/8 درصد بیشتر از تیمار مذکور در شرایط مشابه بود. استفاده از باکتری‌های محرک رشد به همراه درصد مناسبی از کود شیمیایی فسفر ضمن افزایش کارایی کود شیمیایی و حفاظت از خاک، موجب افزایش میزان عملکرد دانه در شرایط تنش آبی می‌گردد.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
Effect of Plant Growth Promoting Rhizobacteria (PGPR) in Yield and Yield Components of Corn Hybrid (540) under Water Stress Condition
نویسندگان [English]
- Seyed Mohamad Reza Ehteshami 1
- mehdi Ramezani 2
1 Assistant Prof.,Dept. of Agronomy and Plant breeding, Faculty of Agricultural Science, Guilan University
2
چکیده [English]
Background and Objectives
Maize is the most important grain crop that has great economic importance and is grown worldwide. The largest areas under maize in Iran are in the regions with semidry and dry climates. Bio-fertilizers differ from chemical and organic fertilizers in the sense that they are cultures of special bacteria which do not directly supply any nutrients to crops. The phenomena of fixation and precipitation of P in soil caused a low efficiency of soluble P fertilizers such as superphosphate. The studies on the application of biofertilizers as replacements or supplements to mineral nutrients in the crop production show economic and ecological justification of their broad practical application and commercialisation of biofertilizers. Given the importance of water and fertilizer in agriculture, research leading to improvements in water efficiency and the use of chemical fertilizers is essential.
Material and Methods
In order to investigate the effect of growth promoting bacteria and different levels of phosphorus chemical fertilizer on grain yield and yield components of corn hybrid (540) under humidity different regimes, an experiment was conducted as Line source based on a randomized complete block design with three replications in Agricultural Education Center of Shahid Naseri in Karaj during 2012 and 2013. Line source was applied to four levels of irrigation (not stress, mild stress, moderate stress, severe stress). Phosphorus factor was in five levels including the application of 100% triple super phosphate fertilizer and without biofertilizer, 75% recommended chemical fertilizers with biofertilizer, 50% recommended chemical fertilizers with biofertilizer, 25% recommended chemical fertilizers with biofertilizer, biofertilizer and without chemical fertilizers, too.
Results
The results of the experiment showed the significant effect of fertilizer treatments on all traits both in the first and second years. In comparison, the effect of different levels of phosphorus fertilizers was investigated by the application of 100% phosphorus fertilizer and without inoculation it had the highest grain yield under non-stress conditions, while with increasing of stress intensity, grain yield in 75% recommended chemical fertilizers with biofertilizer treatment under mild stress, moderate stress and severe stress conditions was 2.1%, 5.5% and 35.8 % higher than the same treatment in similar circumstances, respectively.
Discussions
The result of the experiment showed that the use of plant growth promoting bacteria with a good percentage of the phosphorus chemical fertilizer, while increasing the efficiency of chemical fertilizers and soil conservation, improved grain yield under water stress conditions. The positive effect of bio-fertilizer may also be due to optimum soil pH which facilities maximum utilization of applied micronutrients to crops. Also, use of Biosuper biofertilizer had positive effects on all traits.
کلیدواژه‌ها [English]
- Grian yield
- Line source
- phosphorus
- Pseudomonas strains
- Anabi Milani, A. 2003. Effect of irrigation regimes on yield components and water use efficiency of wheat in saline soil. Soil and Water Science, 16(1): 121-135. [In Farsi]
- 2014. Agricultural Statical letteres, Ministry of Agriculture, Department of Economic and Planning, Center of Information and Communication Technology PP 121. [In Farsi]
- Ansary, M.H., Asadi Rahmani, H., Ardakani, M.R., Paknejad, F., Habibi, D., and Mafakheri, S. 2012. Effect of pseudomonas fluorescent on proline and phytohormonal status of Maize (Zea mays) under water deficit stress. Annals of Biological Research, 3(2): 1054-1062.
- Chapman, S.C., Crossa, K. Basford, E., and Kroonenberg, P.M. 1997. Genotype by environment effects and selection for drought tolerance in tropical maize: Three-mode pattern analysis. Euphytica, 95(1): 11-20.
- Demissie, SH., Muleta, D., and Bercha, G. 2013. Effect of phosphate solubilizing
bacteria on seed germination and seelding growth of Faba Bean (Vicia Faba L). International Journal of Agricultural Research 8(3): 123-136. - Eck, H.V. 1988. Effect of water defficition yield, yield components and water use efficiency of irrigated corn. Agronomy Journal, 78: 1035-1040.
- Edmeades, G.O., Bolanson, J., Banziger, M., and Ortega, A. 1998. Developing drought and low-nitrogen tolerant. Maize Symposium abstracts. Department Agriculture, University of Queensland, Brisbane 4072. Australia.
- Ghazian Tafrishi, SH., Ayenehband, A., Tavakoli, H., Khavari Khorasani, S., and Joleini., M. 2012. Investigating sweet corn (Zea mays L.) yield determining traits under normal irrigation and water deficit stress, using multivariate statical methods. Environmental Stresses in Crop Sciences, 5(1): 95-98. [In Farsi]
- Hanks, R.J., Keller, J., Rasmussen, V.P., and Wilson, G.D. 1976. Line Source sprinkler for continuous variable irrigation-crop production studies. Soil Society American Journal, 40: 426-429.
- Jalilian, A., Ghobadi, R., and Farnia, A. 2011. The investigation effects of fertilizer on the yield and nitrogen use efficiency of corn (SC704) various levels of drought stress and nitrogen. New Idea in Agriculture 5th, Symposium, Khorasgan, Esfahan, Iran. 17-18 Feb. 2011. Islamic Azad University, Khorasgan, Esfahan, Iran. [In Farsi]
- Johnson, D.E., Chaudhuri, U.N., and Kanemasu, E.T. 1983. Statistical analysis of line-source sprinkler irrigation experiments and other nonrandomized experiments using multivariate methods. Soil Science Society American Journal, 47: 309-312.
- Khan, A.A., Jilani, G., Akhtar, M.S., Naqvi, , and Rasheed, M. 2009. Phosphorus solubilizing bacteria: Occurrence, mechanisms and their role in crop production. Journal of Agricultural Biological Sciences, 1: 48-58.
- Lack H., naderi, A.S., Siadat, A., Ayenehband, A., Nour-Mohammadi, Gh., and Moosavi, S.H. 2008. The effects of different levels of irrigation, nitrogen and plant population on yield, yield components and dry matter remobilization of corn at climatical conditions of Khuzestan. Water and Soil Science, 11(42): 1-14. [In Farsi]
- Mahrokh, A. and Khajehpour, M.R. 2010. The effect of irrigation regimes on growth indices and quantitative and qualitative yields of sugar beet. Iranian Crop Sciences Journal, 41(2): 235-246. [In Farsi]
- Marulanda, A., Azcon, R., Ruız, J., Lozano, M., and Aroca, R. 2008. Differential effects of a bacillus megaterium strain on lactuca sativa plant growth depending on the origin of the arbuscular mycorrhizal fungus coinoculated: physiologic and biochemical traits. Journal of Plant Growth Regullar, 27: 10-18.
- Najafi Nejad, H. and Madahian, H. 2004. Effects of different irrigation regimes and plant density on yield and some agronomic characteristics of corn. Seed and Plant, 19(2): 155-172. [In Farsi]
- Peix, A., Rivas-Boyere, A.A., and Mateos, P.F. 2001. Growth promotion of chickpea
and barley by a phosphate solubilizing strain of mesorhizobium mediterraneum under growth chamber condition. Soil Biology and Biochemistry, 33(1): 103-110. - Postel, S. 1995. Last oasis, fasing water scarcity. Vahab zadeh, A. and A. Alizaeh. Jahad-e- Daneshgahi Mashhad, pp: 157. [In Farsi]
- Shoae Hosseini, S.M., Farsi, M., and Khavari Khorasani, S. 2009. Investigation of water deficit stress effects on yield and yield compomemts using path analysis some corn hybrids. Agricultural Science, 18(1): 71-85. [In Farsi]
- Subramanian, K.S., Charest, C., Dwyer, L.M., and Hamilton, R.I. 1997. Effect of mycorrhizaon leaf water potential, suger and P contents during and after recovery of maize. Canadian Journal of Botanic, 75: 1581-1591.
- Yasmin, H., Bano, A., and Samiullah, A. 2013. Screening of PGPR isolates from semi-arid region and their implication to alleviate drought stress. Pakistan Journal of Botany, 45: 51-58.
- Yazdani, M., Bahmanyar, M.A., Pirdashti, H., and Esmaili, M.A. 2009. Effect of phosphate sulubilization microorganisms and plant growth promoting rhizobactria on yield and yield components of corn. International Journal of Biology Life Science, 1: 2.
- Zarabi, M., Alahdadi, I., Akbari, Gh., Abbas., Iran Nejad, H., and Akbari, Gh.A. 2010. Reduction of drought stress effects on yield and yield components of grain corn (Zea mays) using mixed biofertilizers and phosphorus. Journal of Crops Improvement, 12(2): 37-50. [In Farsi]
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