Document Type : Research Paper
Authors
1 M.Sc of Plant Breeding, Faculty of agriculture, Bu-Ali Sina University, Hamedan
2 Associate Professor of plant breeding, Faculty of agriculture, Bu-Ali Sina University, Hamedan, Iran
3 Associate Professor of Horticultural, Faculty of agriculture, Bu-Ali Sina University, Hamedan, Iran
4 Assistant Professor of plant breeding, Faculty of agriculture, Bu-Ali Sina University, Hamedan, Iran
Abstract
In this research, the effect of ovary co-culture on androgenesis efficiency in anther cultures of four cultivars of cucumber was studied. A factorial experiment based on Completely Randomized Design with three replications was used in this study. Cultivars of cucumber (Beta Alfa, Esfahani, Basmanj and Korki), type of culture medium (solid and liquid) and number of cucumber ovaries (0, 10, and 20) were considered as the experimental factors. Different cultivars of cucumber, two type of culture medium, different numbers of ovaries in culture medium, two-way interaction effects and triple interaction effect of cultivar × culture medium × ovary number showed significant differences (p<0/001) for the traits of callogenesis percentage (except for the interaction effect of culture medium × ovary number) and the mean number of embryo per anther. The Use of MS liquid medium without any ovary in Esfahani cultivar (90% callogenesis), solid medium containing 10 ovaries in Korki cultivar (86.66% callogenesis) and also solid medium containhing 20 ovaries in Basmanj cultivar (76.66% callogenesis) showed the highest callogenesis percentage. The Application of 10 ovaries in solid medium in Korki and Esfahani cultivars produced the highest embryogenic calli (60% and 56.66%, respectively). Furthermore, the highest mean number of embryo per anther obtained with 10 ovaries in solid medium in both cultivars of Esfahani and Korki (0.76 embryo per anther). Finally, use of solid MS medium containing 10 ovaries in Beta alfa, Esfahani and Korki cultivars and also 20 ovaries in Basmanj cultivar increased the embryo production in cucumber anther culture.
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