Document Type : Research Paper
Authors
1 M.Sc. in Plant breeding, Faculty of Agriculture, Shahid bahonar University of Kerman, Kerman, Iran
2 Department of Biotechnology, Faculty of Agriculture, Shahid bahonar University, Kerman, Iran.
Abstract
Nowadays providing silver nano particles being done in various chemical ways, in which we face different major problems (e.g., instsbility of solution and the hi-tech equipments) is vitally needed. Thus, researchers have tried the green systems which have the least amount of bio dangers, and are easier to use. The silver nanoparticles were investigated by using green fruit extract of Melia azedarach. The effect of extract and silver nitrate concentration on amount of silver nanoparticles absorbance were evaluated as a factorial experiment based on a completely randomized design with three replications. X-ray diffraction was used to confirm the crystalline nature of the particles. Thesize and particles size distribution was assessed by Transmission electron microscope. Then antibacterial properties of synthesized nanoparticles were studied using the dick method. Nanoparticle synthesis relative monodisperse, hesagonal with an average size of 20 nm in Melia azedarach green fruit extract was reported. A according to the results, the interaction of silver nitrate and extract concentration was significant on amount of synthesis absorbance. The antibacterial activities of the synthesized nanoparticles were effective on Staphylococcusaureus and Pseudomonas aerauginos. Given that antibiotic resistance is a common problem to destroy the bacteria, nanomaterials as materials can be used for containment and eradication of these bacteria, but the resistance of clinical isolates in comparison with standard strains should be investigated.
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