1
M.Sc. Student in Plant Breeding, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
2
Departments of Biotechnology and Plant Breeding, Sari Agricultural Sciences and Natural Resources University, Genetics & Agricultural Biotechnology Institute of Tabarestan, Sari, Iran
Combination of classical breeding with molecular marker-assisted selection speeds up the development of new breeding lines. Lack of appropriaterestorervarieties, the low number ofsuitable linesand their narrow genetic base are fundamental problemsin hybridrice production. This study was performed with the aim of identification and selection ofplantswithrestorefertility genes in segregating populations of rice. Therefore study of nucleus genome of selected lines was conducted along with molecular studies for the identification of restorer lines in two convergent populations of rice Nemat ×Pajohesh// IR58110 ×Pajoheshand Nemat ×Pajohesh// IR60819 ×Pajoheshand two populationsofPajohesh × IR60819 and IR58110 ×Pajohesh. The experiments were followed for twoyears and the results showed that the linesR5, R7, R9, R28andR45had desirable performance in the pollen and spicklet fertilitytest. Molecular analysisperformed onselectedlines andgenotypes showed that the markers RM171, RM3148, RM1 and RM258 presented good banding patterns. Therefore, the lines R3, R5, R7, R9, R18, R25, R27, R28, R29 and R45 were identified as positive at least for two markers representing similar patterns torestorercheck (IR50). Thus,the results of pollen and spikes fertilitytests and molecular studies demonstrated that lines R5, R7, R9, R28andR45are potentialrestorers. Furtherstudies in futuregenerations of theselinescanbe used as desirable lines inhybridseed production.
Eidi Kohnaki, M., Kiani, G. & Nematzadeh, G. (2015). Morphological and Molecular Selection of Fertility Restorer Gene(s) in Segragating Populations of Rice. Plant Productions, 38(2), 89–98. https://doi.org/10.22055/ppd.2015.11322
MLA
Eidi Kohnaki, M., Kiani, G., & Nematzadeh, G. "Morphological and Molecular Selection of Fertility Restorer Gene(s) in Segragating Populations of Rice", Plant Productions, 38, 2, 2015, 89–98. doi: 10.22055/ppd.2015.11322
HARVARD
Eidi Kohnaki, M., Kiani, G., & Nematzadeh, G. (2015). 'Morphological and Molecular Selection of Fertility Restorer Gene(s) in Segragating Populations of Rice', Plant Productions, 38(2), pp. 89–98. doi: 10.22055/ppd.2015.11322
CHICAGO
Eidi Kohnaki, M., Kiani, G. & Nematzadeh, G., "Morphological and Molecular Selection of Fertility Restorer Gene(s) in Segragating Populations of Rice." Plant Productions, 38 2 (2015): 89–98, doi: 10.22055/ppd.2015.11322
VANCOUVER
Eidi Kohnaki M., Kiani G., Nematzadeh G. Morphological and Molecular Selection of Fertility Restorer Gene(s) in Segragating Populations of Rice. Plant Productions. 2015;38(2):89–98 (In Persian). doi: 10.22055/ppd.2015.11322
