Authors
1 M.Sc. West-Azarbayjan Branch of Academic Center for Education, Culture & Research (ACECR) Urmia, Iran
2 M.Sc. Department of Horticulture, Faculty of Agriculture, Urmia University, Urmia, Iran
3 Department of Horticulture, Faculty of Agriculture, Urmia University, Urmia, Iran
Abstract
Somatic embryogenesis is an alternative method for genetic improvement of commercially important cultivars and is an effective technique for plant propagation.In this research, effects of different factors were studied on embryogenic callus formation from different explants (petal, filament, receptacle, leaf and stem) in M.26 apple dwarf rootstock. MS basal medium supplemented with different concentrations of NAA and 2,4-D (0, 1, 2, 3 and 4 mg/l) was used for embryogenic callus induction. Auxin treatments showed significant effects on callus induction and percentage of induced explants in mediums containing NAA was higher than that of mediums containing 2,4-D. Petal explantson medium with 4 mg/l NAA had higher percentage of embryogenic callus. Higher percent of globular embryosinduction was observed in B3 treatment (MS+1 mg/l TDZ+1 mg/l ABA+0.5 mg/l GA7).
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Main Subjects
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