Plant Productions

Effect of Clone and Growth Regulators on Callus and Bulblet Formation Using Daughter Bulb Explants of Fritillary (Fritillaria imperialis L.)

Saadollah Alizadeh Ajirlo; mohammad kargar

Volume 39, Issue 2 , September 2016, , Pages 81-94

https://doi.org/10.22055/ppd.2016.12058

Abstract

Introduction: Micropropagation is the best approach to propagation of Fritillaria and prevention of its extinguishing. The effects of auxin and cytokinin combinations on callus and ... Read More Introduction: Micropropagation is the best approach to propagation of Fritillaria and prevention of its extinguishing. The effects of auxin and cytokinin combinations on callus and bulblet production using explants taken from daughter bulb scale of two fritillary clones collected from Arjan and Koohrang plains of Fars province were studied.Materials and methods: For this purpose, 3 types of growth regulator combinations containing three levels of NAA (0, 0.5 and 1 mg L-1) with one of the cytokines (2ip, Kinetin and TDZ) at three levels (0.5, 1.0 and 2 mg L-1) were applied. The base medium was MS containing 4.5 percent sucrose and 8 g/l agar. To evaluate the effects of these factors, experimental design of completely randomized factorial with 4 replications and 5 explants per repeat was used. Background and Objectives Micropropagation is the best approach to propagation of Fritillaria and prevention of its extinction. The effects of auxin and cytokinin combinations on callus and bulbletproduction using explants taken from daughterbulb scale of two fritillary clones collected from Arjan and Koohrang plains of Fars province were studied. Materials and methods For this purpose, 3 types of growth regulator combinations containing three levels of NAA (0, 0.5 and 1.0 mg L-1) with one of the cytokines (2ip, Kinetin and TDZ) at three levels (0.5, 1.0 and 2.0 mg L-1) were applied. The base medium was MS containing 4.5 percent sucrose and 8 g/l agar. To evaluate the effects of these factors, an experimental design of completely randomized factorial with 4 replications and 5 explants per repeat was used. Results Analysis of data revealed that the kinetin and 2ip had no significant effect on callus induction percentage while TDZ was significant on this parameter. The highest callus production was observed in medium containing 0.5 mg L-1 NAA combined with 0.5 mg L-1 TDZ. Using this treatment, 60 percent of explants produced the callus. The most number of direct and indirect regenerated bulblets were recorded in medium complemented with 1.0 mg/l NAA and 1.0 mg L-1 Kinetin. The mean of 5.5 bulbletper explants was obtained in this treatment. Discussion Considering the effectiveness and cost of NAA and environmental issues, its lower concentration is recommended for bulblet regeneration in this plant.

Effect of shade, organic matter and planting time on morphological and physiological characteristics of lisianthus (Eustoma grandiflorum) (Raf.) Shinn.

M. Rahnemoon far; N. Etemadi; A. Nikbakht; M. Gheisari; M.R. Sabzalian

Volume 37, Issue 3 , December 2014, , Pages 1-11

Abstract

In order to investigate the effect of shade, organic materials and planting time on characters of Lisianthus, a completely randomized design experiment was conducted in greenhouse of ... Read More

The Effects of Different Amounts of Natural Zeolite on Vegetative and Reproductive Characteristics of Narcissus tazetta L. cv. Shahla

F. Nazari; H. Farahmand; M. Ghasemi Ghehsareh3

Volume 37, Issue 2 , September 2014, , Pages 39-48

Abstract

There exist a lot of reports about the positive and significant effect of natural zeolite on vegetative and reproductive characteristics of plants such as cereals, vegetable transplants, ... Read More

The evaluation of some growth and physiological indicators and enzymatic activities in Conocarpus erectus L. under oil sewage and calcium oxide treatments

E. Rezazadeh Katehsari; R. Fotouhi Ghazvini; V. Jafarian; l. abdoli

Volume 37, Issue 2 , September 2014, , Pages 63-73

Abstract

The identification of plants that are able to grow in the presence of oil pollutants or be effective in reducing pollution is very important. The experiment was acnducted in order to ... Read More

The study of the effect of some natural preservative solutions on the vase life of Cycad’s leaves

M. Abshahi; H. Zarei; A. Ghasem-Nejhad; M. Aghdasi

Volume 37, Issue 2 , September 2014, , Pages 75-87

Abstract

In order to investigate vase life of cut foliages of cycad, the current research was carried out using various treatments of natural preservative solutions. Silver nitrate and sucrose ... Read More

Effects of regulated irrigation on some physiological characteristic of Frankenia leavise L.

S. Chegah; M. Chehrazi; M. Albaji

Volume 37, Issue 1 , June 2014, , Pages 71-80

Abstract

Cover plants application has several advantages such as beauty in landscape, inhibition of soil erosion and the best way of using water. Frankenia (Frankenia Leavise) is one of ... Read More

Effect of row planting design and planting distance on flowering and quality of gladiolus cut flower (Gladiolus hybrida cv. Oscar (

M.H. Daneshvar; M. Heidari

Volume 36, Issue 1 , June 2013, , Pages 129-140

Abstract

The increasing of planting density of bulbous flowers is one of the most important step for more production of ornamental plants. One of the possible methods for increasing density, ... Read More The increasing of planting density of bulbous flowers is one of the most important step for more production of ornamental plants. One of the possible methods for increasing density, is cultivation of bulb plants in multiple rows pattern. In this research, the effect of one, two, and three row planting design bed were investigated on flowering and quality of Gladiolus (Gladiolus hybrida cv.Oscar) cut flower in climatic condition of Mollasani in Khuzestan province, located in south west of Iran on 1388 through 1389.. The treatments were the cultivation of corms in on row of 30 × 20 cm (control) and two and three-bed row (20 cm on row, 30 cm between main row and/or pattern with distance of 15, 20 and 25 cm between subordinate row). The results showed that cultivation of corm in three-row bed pattern with distance of 25 cm between subordinate row and/or cultivation of two-bed and three-bed row in distance between subordinate rows of 15 and 20 cm causes significant decreases on weight of cut flower, stem diameter and height of spike. Height of cut flower was not significantly different only in treatment of two-bed row with distance of 25 cm between subordinate rows when compared to control. The number of cormels were significantly decreased In treatment of three-bed cultivation pattern with distance of 25 cm between subordinate rows, as compared to control. The number of floret, only, in cultivation pattern of two-bed and three-bed row with distance of 15 cm between rows had significantly decreased when compared to control treatment. These treatments had no significant effect on diameter of cut flower, height of spike, diameter, width and weight of corms. The results indicated that the cultivation of gladiolus in pattern of multiple rows is possible but the decrease of distance between rows, reduce the quality of cut flower. It strongly suggested that more researches require to accomplish on various distances on subordinate rows.

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Main Subjects = H: Ornamental Plants

Effect of Clone and Growth Regulators on Callus and Bulblet Formation Using Daughter Bulb Explants of Fritillary (Fritillaria imperialis L.)

Abstract

Effect of shade, organic matter and planting time on morphological and physiological characteristics of lisianthus (Eustoma grandiflorum) (Raf.) Shinn.

Abstract

The Effects of Different Amounts of Natural Zeolite on Vegetative and Reproductive Characteristics of Narcissus tazetta L. cv. Shahla

Abstract

The evaluation of some growth and physiological indicators and enzymatic activities in Conocarpus erectus L. under oil sewage and calcium oxide treatments

Abstract

The study of the effect of some natural preservative solutions on the vase life of Cycad’s leaves

Abstract

Effects of regulated irrigation on some physiological characteristic of Frankenia leavise L.

Abstract

Effect of row planting design and planting distance on flowering and quality of gladiolus cut flower (Gladiolus hybrida cv. Oscar (

Abstract