Plant Productions - Articles List

Investigating the effects of post-harvest application of nanocellulose/carvacrol composite coating on the physico-chemical characteristics of Physalis fruit with calyx

Leila Fereidoni; Abdollah Ehtesham Nia; Hassan mumivand; Mohamadrez Raji

Articles in Press, Accepted Manuscript, Available Online from 10 July 2023

https://doi.org/10.22055/ppd.2023.42926.2078

Abstract

IntroductionPhysalis (Physalis peruviana L.) belongs to the Solanaceae family and Its fruit is berry, round and orange in color. This fruit is climacteric and after reaching physiological ... Read More IntroductionPhysalis (Physalis peruviana L.) belongs to the Solanaceae family and Its fruit is berry, round and orange in color. This fruit is climacteric and after reaching physiological maturity, it can be harvested as a mature green. Since small fruits are consumed fresh and also because of their sensitivity to spoilage pathogens, physiological disorders. On the other hand, food safety is one of the important issues related to fresh fruits and vegetables. Most of the natural compounds are degradable and can be a safe compound for human health and the environment and an alternative to synthetic compounds. Research has shown that the incorporation of bioactive compounds such as essential oils, plant extracts and nanocomposite increases the active effect as a packaging for fruit and reduces the rate of fruit deterioration.Materials and MethodsThis research was done in the laboratory of Lorestan university (Khorramabad, 33° 26 ´N latitude and 48 °15´E longitude and at an altitude of 1148 meters above sea level). In this study, physalis fruits from Khorramabad research greenhouse, nanocellulose gel extracted from Bergan conifer pulp from Nano Novin Polymer Company, pure carvacrol by GC/MS analysis from Khorrman Pharmaceutical Company and the combination of nanocellulose with carvacrol, in The laboratory was prepared. The experiment was conducted as a factorial with two factors in the form of a complete random design with 4 replications. The first factor is the type of material in specific concentrations at nine levels (Control, Car 0.3%, Car 0.6%, CNF 0.5%, CNF 1.5%, Car 0.3+CNF 0.5%, Car 0.6+CNF 0.5%, Car 0.3+CNF 1.5%, Car 0.6+CNF 1.5% and the second factor of storage time at four levels (0, 45, 90 and 135 days). First, physalis fruits were washed with distilled water, and then the excess leaves and damaged fruits were separated. The fruits were placed in the desired suspension for 2-3 minutes. After applying the treatment, the fruits were placed in a refrigerator with a temperature of 4 degrees and a relative humidity of 90%.Results and DiscussionThe results showed that the treated fruits with nanocellulose and carvacrol showed less weight loss and had higher tissue firmness. Examining the biochemical characteristics also showed the positive effect of the treatments on the desired parameters, so that the most TA and vitamin C and the least amount of acidity were obtained in the nanocellulose and nanocellulose/carvacrol combination treatments. Also, the treatments used reduced the TSS and the fruit TSS/TA, and the combined treatments had the lowest values. By creating a layer on the surface of the fruit, nanocellulose coating prevents water evaporation and reduces metabolic and respiration processes. The amount of fruit softening is directly related to the amount of decomposition of pectin compounds through the enzyme activity of methylesterase and polygalactronase during ripening. The used coatings form a thin layer on the surface of the fruit, which can reduce gas exchange and the rate of respiration. Nanocellulose/carvacrol composite coatings lead to an increase in the activity of cytochrome oxidase by reducing internal oxygen and this enzyme increases the rate of ascorbic acid decomposition. ConclusionIn general, the results of the research showed that the investigated characteristics of physalis fruit were influenced by the treatments used. As treated samples, weight loss was less, acidity, soluble solids and taste index were lower, tissue firmness, titratable acids and vitamin C were higher. According to the general results, it can be stated that the application of nanocellulose/carvacrol composite treatments, especially the Car 0.6+CNF 1.5% treatment, showed more favorable results than the application of each one alone in most features.

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Postharvest sour cherry quality maintenance and improvement of antioxidant capacity by treatment with malic acid

mahla sanati; soheila mohammadrezakhani

Volume 46, Issue 2 , September 2023

https://doi.org/10.22055/ppd.2023.43467.2095

Abstract

IntroductionFruits are one of the most valuable garden products that play an important role in providing nutritional needs and human health. This group of agricultural products are ... Read More IntroductionFruits are one of the most valuable garden products that play an important role in providing nutritional needs and human health. This group of agricultural products are perishable due to high humidity, high respiration and transpiration, and most of them are destroyed in the post-harvest period. In this study, organic acids including malic acid with different concentrations were used to protect cell membranes, maintain quality and increase shelf life during the postharvest period in sour cherry fruits.Materials and Methods: Sour cherry fruits were treated with 0, 2 and 4% malic acid and stored at 1°C with 85% relative humidity for 16 days. Traits such as chilling injury, hydrogen peroxide, lipid peroxidation, ion leakage, antioxidant capacity and antioxidant enzymes including peroxidase, catalase, superoxide dismutase, ascorbate peroxidase were measured. Results and Discussion: The results showed that the application of malic acid with both concentrations and at any time intervals from storage led to a reduction in chilling injury, hydrogen peroxide, lipid peroxidation, ion leakage compared to control samples. On the other hand, in fruits treated with malic acid, antioxidant capacity and activity of antioxidant enzymes including peroxidase, catalase, superoxide dismutase, ascorbate peroxidase have increased. Conclusion: The general results show that malic acid with a concentration of 4% at each time interval has favorable effects on improving the antioxidant activity of the sour cherry fruits during the storage period.IntroductionFruits are one of the most valuable garden products that play an important role in providing nutritional needs and human health. This group of agricultural products are perishable due to high humidity, high respiration and transpiration, and most of them are destroyed in the post-harvest period. In this study, organic acids including malic acid with different concentrations were used to protect cell membranes, maintain quality and increase shelf life during the postharvest period in sour cherry fruits.Materials and Methods: Sour cherry fruits were treated with 0, 2 and 4% malic acid and stored at 1°C with 85% relative humidity for 16 days. Traits such as chilling injury, hydrogen peroxide, lipid peroxidation, ion leakage, antioxidant capacity and antioxidant enzymes including peroxidase, catalase, superoxide dismutase, ascorbate peroxidase were measured. Results and Discussion: The results showed that the application of malic acid with both concentrations and at any time intervals from storage led to a reduction in chilling injury, hydrogen peroxide, lipid peroxidation, ion leakage compared to control samples. On the other hand, in fruits treated with malic acid, antioxidant capacity and activity of antioxidant enzymes including peroxidase, catalase, superoxide dismutase, ascorbate peroxidase have increased. Conclusion: The general results show that malic acid with a concentration of 4% at each time interval has favorable effects on improving the antioxidant activity of the sour cherry fruits during the storage period.The general results show that malic acid with a concentration of 4% at each time interval has favorable effects on improving the antioxidant activity of the sour cherry fruits during the storage period.The general results show that malic acid with a concentration of 4% at each time interval has favorable effects on improving the antioxidant activity of the sour cherry fruits during the storage period.

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Evaluation of the Effects of Calcium Nitrate and Potassium Phosphite on the Storage Life and Some Quality Traits of Thomson Navel Orange

Amirali Mohammadi; Mehrdad Shahabian; Mahmoud Reza Ramezanpour; Shokrollah Hajivand

Volume 45, Issue 2 , September 2022, , Pages 181-192

https://doi.org/10.22055/ppd.2021.35965.1959

Abstract

IntroductionOranges belong to the Citrus (family Rutaceae) and are an important source of antioxidants, including phenolic compounds and vitamin C. These compounds have a protective ... Read More IntroductionOranges belong to the Citrus (family Rutaceae) and are an important source of antioxidants, including phenolic compounds and vitamin C. These compounds have a protective effect against various diseases such as cancer and cardiovascular. Since there is a possibility of decay and rot of Citrus in all stages of harvest and post-harvest, the use of mineral elements may have an important effect on increasing the quality of the fruit and its shelf life. This effect varies depending on the species and cultivar of Citrus. Mineral elements, especially potassium and calcium, affect fruit growth and Citrus fruit quality. Materials and MethodsTo investigate the effect of spraying, a research was conducted in Qaemshahr city in 2019, which in this study, manual foliar application of calcium nitrate (0, 2 and 4 g / l) and potassium phosphite (0, 1.5 and 3 g / l) was done in the late October in three stages with a week interval. Then, one week after the last treatment, the harvesting operation was performed with garden shears. After washing, the fruits were kept in normal temperature for 3 months. Various parameters such as morphology (percentage of weight loss, firmness, percentage of decay, etc.), biochemical (total soluble solids (TSS), titratable acidity (TA) and acidity of fruit juice (pH)), antioxidant (percentage of inhibition and vitamin C), enzyme (ascorbate peroxidase and superoxide dismutase) and marketability traits were evaluated. Results and DiscussionThe results showed that the application of higher concentrations of calcium nitrate and potassium phosphite improved the measured traits at harvest day. The use of these materials has also increased the shelf life and quality of the fruit during storage. Fruits treated with calcium nitrate and potassium phosphite had more antioxidant properties on the day of harvest. The highest amount of vitamin C and inhibition percentage were observed in the treatment of 4 g / l calcium nitrate and 3 g / l potassium phosphite at harvest day. Treated fruits were more firm and less rotten during storage than control ones. Also, the treated fruits had more soluble solids and titratable acidity. Also, during storage, the quality of the fruit and its marketability decreased, but fruits treated with calcium nitrate and potassium phosphite received higher scores from the evaluators. The use of calcium increases the strength of the membrane and reduces the activity of enzymes that relax the cell wall, which therefore increases the firmness of the fruit and reduces the weight loss and rot of fruits during storage. The use of potassium phosphite has also been effective in reducing caries. Calcium along with potassium play a role in photosynthesis and cause the accumulation of sugars. Vitamin C is associated with a decrease during storage, due to the use of ascorbic acid as an ascorbate peroxidase cofactor. Finally, it seems that the marketability of the fruit has increased due to the increase of quality traits such as sugars, due to the use of calcium nitrate and potassium phosphite. ConclusionThe storage of Thomson Novel oranges was affected by the treatments used, and the highest firmness, weight and quality characteristics of the fruit were obtained in the highest amount of calcium nitrate and potassium phosphite. Also, the rate of caries has decreased significantly with the use of these treatments.

Effect of Different Concentrations of Spermidine at Different Harvesting Times on the Quality of Apricot Fruits ”Shahroudi“ Cultivar

Jassim Taha; Jafar Hajilou

Volume 43, Issue 3 , November 2020, , Pages 409-418

https://doi.org/10.22055/ppd.2019.28168.1703

Abstract

Abstract Background and Objectives Apricot is one of the most important horticultural crops because of its attractive taste and nutritive value. Different cultivars of ... Read More Abstract Background and Objectives Apricot is one of the most important horticultural crops because of its attractive taste and nutritive value. Different cultivars of apricot contain highly variable content of ascorbic acid, cartenoids, and phenolic compound,which are good sourecs of antioxidant. Fuit quality is an important trait for concumers and producers. The purpose of this work was to estimate the effects of pre-harvest application of polyamines on quality characteristics of apricot fruits at the end of fruit ripening process. Materials and Methods The experiment was carried out as a factorial based on the randomized complete block design (three concentrations of spermidine at three harvesting time) with four replications. The selected branches of trees were sprayed as a pre-harvesting application method by three concentrations of spermidine (0, 2, and 3 mili molar) 7 days before commercial harvesting time. Fruits were harvested during harvesting times (3 days interval) and then some fruit characteristics such as total soluble solids (TSS), total ccidity (TA), vitamin C, firmness of fruit tissue, pH of fruit extract, total phenol, flavonoid, and total antioxidant capacity were measured in the laboratory of flower biology and fruit growth development at the department of horticulture, faculty of agiculture, university of Tabriz. Results The results showed that the effects of different harvesting times were significant regarding all traits. The best results were observed in 3 mM spermidine and this treatment improved all the measured characteristics of fruit. The highest value of total antioxidant capacity (3.01%), vitamin C (3.29%), and fruit firmness (3.27%) were found in 3 mM spermidin at three harvesting times. In some characteristics such as TSS and TA, there was no significant difference between 2 and 3 mM spermidine, but among treated and non-treated fruit a full significant difference was observed. We assume that the second harvesting time can be the best harvesting time regarding fruit quality parameters. During fruit ripening, the amount of TSS and TA increased, while firmness, total phenolic, and flavonoids compounds and total antioxidant capacity decreased gradually. Discussion Fruit physicochemical properties are very important for understanding the product behaviour during the harvesting, transporting, packaging, and storing of fruit crops. The results of this study showed that the pre-harvesting application of polyamines delayed the ripening and increased the quality of fruit during the different harvestings times.

Effect of Harmel Extract on Bacterial Population in Vase Solution and Vase Life of ‘Stanza’ and ‘Pink Elegance’ Cultivars of Gerbera Cut Flower

Meisam Mohammadi; Mitra Aelaei; Mehdi Saidi

Volume 43, Issue 2 , September 2020, , Pages 171-184

https://doi.org/10.22055/ppd.2019.27785.1680

Abstract

Abstract Background and ObjectivesGerbera is one of the important cut flowers in the floriculture industry, but it is sensitive to early wilting and neck bending which reduce its ... Read More Abstract Background and ObjectivesGerbera is one of the important cut flowers in the floriculture industry, but it is sensitive to early wilting and neck bending which reduce its postharvest vase life. In cut flowers, water deficits are created when transpiration is more than water absorption. Therefore, petal wilting and neck bending increase in these flowers. These conditions are mainly due to occlusion of the vascular bundle, which can occur in different ways, such as the activity of pathogenic bacteria and other microorganisms in vascular bundle. In recent years, due to the adverse effects of chemical antimicrobial compounds on human health and the environment, many studies have been conducted to find replacement materials to maintain the quality and vase life of cut flowers in postharvest. Therefore, in this study, the effect of different concentrations of harmel aqueous-alcoholic extract (Peganum harmala L.) on bacterial population and vas life of ‘Stanza’ and ‘Pink Elegance’ cultivars of Gerbera cut flower were studied. Materials and MethodsThe effect of concentrations 0, 0.25, 0.5, 0.75 and 1% of the aqueous-alcoholic extract of Harmel plant was studied in a factorial experiment based on completely randomized design with four replications. At harvest time, 3, 6 and 9 days after harvest (at 20 °C and 70% relative humidity), the effect of treatments on the bacterial population of the vase solution and end stem, vase life and some characteristics of ‘Stanza’and ‘Pink Elegance’ Gerbera cut flowers were studied. ResultsThe results showed that the inhibitory effect of harmal extracts on gram-positive bacteria was higher than gram-negative bacteria. Also,‘Stanza’cultivar had better quality and longer vase life than ‘Pink Elegance’ cultivar. By increasing the vase life of flowers, the quality of these flowers decreased. In the first place, however, concentrations of 0.25% and then 0.5% of the harmal extract performed better than other treatments in various aspects: first, they reduced the bacterial solution, stem end, and electrolyte leakagein stem and petals; second, they preserved quality (discolored of vase solution, discolored of petals and stem), relative fresh weight, stem diameter, totalsolublesolids, catalase activity and phenylalanine ammonia lyase activity; and finally, they increased vase life of gerbera flowers in both cultivars. Concentrations of 0.75% and 1% increased allelopathic substances in cut flower. While these treatments decreased the bacterial population in vase solution and stem end, they did not have a significant effect on the quality and vase life of gerbera cut flowers. DiscussionThe results of this study showed that the different concentrations of harmal extract significantly reduced the growth of microbial agents, including the bacterial population, but by increasing the concentration of the harmal extract, the allelopathic effects of this plant extract on gerbera cut flowers were harmful and did not affect their vase life and quality. Therefore, the concentration of 0.25% then 0.5% reduced the bacterial populations in vase solution and stem end of cut flowers and preserved the quality and vase life of ‘Stanza’ and ‘Pink Elegance’ cultivars. Therefore, the use of low concentrations of the harmel aqueous-alcoholic extract is recommended to reduce the microbial population of the vase solution and maintain the quality and vase life of the gerbera cut flowers.

Effect of Gamma-Amino-Butyric Acid (GABA) Foliar Application on Chilling and Postharvest Quality of Tomato (cv. Newton)

Lolav Zarei; Mahmoud Koushesh Saba; Yavar Vafaee

Volume 43, Issue 2 , September 2020, , Pages 199-212

https://doi.org/10.22055/ppd.2020.27796.1681

Abstract

Abstract Background and Objectives Tomatoes are considered the favorite and most widely consumed vegetables in the world since their fruit is rich in minerals, vitamins and antioxidant ... Read More Abstract Background and Objectives Tomatoes are considered the favorite and most widely consumed vegetables in the world since their fruit is rich in minerals, vitamins and antioxidant compounds. Tomato fruit is harvested after physiological maturity at different color stages based on consumption type. Mature unripe tomato fruit could be stored for long time and be ripened according to market demand, but this tropical product is susceptible to low temperature, which imposes serious constraints in postharvest period and supply chains. Several techniques or chemical treatments have been used to decrease low temperature deleterious effects. Gamma-mino butyric acid (GABA), as an efficient tool, could increase plant resistance to chilling injury (CI) during postharvest life. This study was carried out to evaluate the effect of pre-harvest GABA spray on postharvest quality changes and chilling of tomato fruit during cold storage. Materials and Methods In this study, the effect of pre-harvest application of GABA at three concentrations (0, 5 and 10 mM) on postharvest quality and chilling of tomato cv. Newton was evaluated. The spray was carried out 2 weeks before harvest. Fruits were harvested after physiological maturity when the fruit color changed from pink to red. During storage period at 4 °C and 90-95% relative humidity, some fruit quality attributes such as weight loss, firmness, chilling injury of tomato fruit, soluble solid content (SSC), titratable acidity (TA) and fruit bioactive constituent were analyzed at days 0, 5, 10, 15 and 20. Results Chilling in control fruit was 25% at final assessment, while in 10 mM GABA treatment it was only 8.33% at the same time. GABA treatments decreased the rate of weight loss and retained fruit firmness during storage. Also, vitamin C and titratable acidity in GABA treatments were higher than those of untreated fruit. Fruit lycopene content increased during fruit storage in both treated and untreated fruit, but the value was greater in GABA treatments than the control. SSC increased during fruit storage, but it was greater in control than the treatments. While superoxide dismutase enzyme activity decreased in both treated and untreated fruit, the rate of decline in control fruit was greater than GABA treatments. Discussion Pre-harvest foliar application of GABA ameliorated chilling injury induced by cold storage, maintained quality properties of tomato fruit, and increased lycopene content. GABA treatments probably lessened deteriorate effect of low temperature through keeping fruit cell integrity and higher bioactive constituent levels.

Effect of Salicylic Acid on the Quality Characteristics of Apple Fruit (Golden Delicious and Red Delicious) During Storage Period

Zahra Sedaghati; Fatemeh Nazoori; Seyed Hossein Mirdehghan

Volume 43, Issue 1 , June 2020, , Pages 39-52

https://doi.org/10.22055/ppd.2019.24821.1577

Abstract

Abstract Background and Objectives Apple has a long-term supply throughout the year because of its high storage capacity. Sometimes keeping this fruit in the cold storage room ... Read More Abstract Background and Objectives Apple has a long-term supply throughout the year because of its high storage capacity. Sometimes keeping this fruit in the cold storage room for a long time reduces the quality and marketability, thus making it unattractive for consumers. Recently, there has been an increasing interest in the use of natural compounds to maintain fruit quality and extend its shelf life. Salicylic acid (SA), a widely distributed compound in plants, belongs to a group of phenolic compounds. It could be considered an endogenous plant growth regulator involved in the regulation of physiological processes and disease resistance mechanisms. Materials and Methods To solve this problem, the effect of different concentrations of salicylic acid on the storage of golden and red delicious apple fruits was examined at harvest, 45, 90, 135 and 180 days after storage. Treatments included no washing, distilled water, and salicylic acid 1, 2, and 3 mM for half and 1 hours in cold storage room at 1 ± 2° C. At the end of each storage period, traits such as fruit firmness, vitamin C, TSS/TA, phenol, mealy fruit, flavor (panel test), chilling index and antioxidant capacity were evaluated in three storage periods (0, 90 and 180 days after storage). A factorial experiment based on a completely randomized design was conducted. In both cultivars, harvest index coincided with 170 days after full bloom. Results Results showed that chilling injury, mealy and fruit ripening reduced by salicylic acid treatment and flavor maintained. Antioxidant capacity and phenol content increased by salicylic acid treatment during the storage, but no effect on vitamin C and fruit firmness was observed. During storage, maximum of chilling injury and flavor was related to yellow apple fruit and the maximum of tissue and skin firmness, vitamin C and antioxidant activity belonged to red apple cultivar. The use of 2 mM salicylic acid for half an hour was more effective than other treatments in preserving the quality and storage of fruit of both cultivars. Discussion The results of this study showed that at the end of the storage period the amount of mealy fruit and flavor increased, while skin and tissue firmness decreased significantly. The effects of salicylic acid include a decrease in mealy fruit and chilling injury, as well as an increase in phenolic compounds and maintenance of the antioxidant activity, flavor and TSS/TA, which ultimately increased shelf life of apple fruits. High quality and storage capacity were related to red rather than yellow apple.

Effect of Silicon Nutrition on Strawberry cv. Camerosa Yield and Growth in Outdoor Hydroponic System

Kamran Ghasemi; Mehdi Ghajar Sepanlou; Mehdi hadadinejad

Volume 43, Issue 1 , June 2020, , Pages 93-106

https://doi.org/10.22055/ppd.2019.26611.1634

Abstract

Abstract Background and Objectives Strawberry is one of the important small fruits worldwide because of its high economical value. Mazandaran is ranked second among Iran’s ... Read More Abstract Background and Objectives Strawberry is one of the important small fruits worldwide because of its high economical value. Mazandaran is ranked second among Iran’s strawberry prodcing provinces with about 14581 tones of strawberry yearly. This research aimed to evaluate the effect of different silicon sources on the reproductive and vegetative growth of strawberry in an outdoor hydroponic system. Materials and Methods This experiment carried out in a factorial format with two factors consisting of growth media (double medium: 1 cocopeat + 1 perlite, and triple medium: 1 cocopeat + 1 perlite + 1 rice husk) and silicon spray (control, 50 mg/L Si from sodium silicate, 100 mg/L Si from sodium silicate, 50 mg/L Si from potassium silicate, 100 mg/L Si from potassium silicate). It was performed in Sari Agricultural Sciences and Natural Resources University at 2016. Different characteristics including silicon concentration in the leaf, aerial fresh and dry weight, leaf number, fresh and dry weight of crown, fresh and dry weight of root, yield, weight of single fruit, number of the produced fruits in each plant were evaluated. Results Potassium silicate 100 increased silicon concentration of strawberry leaf significantly. Leaf number of the plant and aerial dry weight in triple medium were significantly higher than the double medium. High rate of potassium silicate in the triple medium caused lower crown diameter in comparison with potassium silicate 50 in the same medium. However, in the double medium, the potassium silicate 50 was the best treatment for crown diameter. Both fresh and dry weight of strawberry roots were the highest in the double medium and potassium silicate 50 treatment. Single fruit weight was maximum in the double medium and potassium silicate 100, which was not significantly different from potassium silicate 100 in triple medium. Fruit dry matter in the double medium and potassium silicate 50 treatments was more than other treatments. Fruit size was affected by medium, so that fruit size in the double medium was more than the triple medium significantly. Discussion Sodium toxicity in sodium silicate treatment, low rate of silicon in potassium silicate 50 treatment, and lack of rice husk in the double medium could all negatively affect different physiological aspects of strawberry growth; or at least, they hindered achieving positive results. In summary, it can be concluded that the double medium and potassium silicate 100 can be recommended for higher yield and big strawberry fruit, but the triple medium and potassium silicate are useful treatments for vegetative growth specially for more crown diameter and root growth.

Evaluation of the Accumulation of Nitrate and Nitrite in Vegetables Grown in Different Regions of Ahvaz

Naser Alemzadeh Ansari; Dariush Sharif Manesh; Sadegh Mousavi fard

Volume 42, Issue 3 , December 2019, , Pages 333-344

https://doi.org/10.22055/ppd.2019.26457.1626

Abstract

Abstract Background and Objectives Khuzestan Province is one of the most important areas that provide vegetables throughout winter and spring in Iran. The vegetables are ... Read More Abstract Background and Objectives Khuzestan Province is one of the most important areas that provide vegetables throughout winter and spring in Iran. The vegetables are mainly picked up in the afternoon, so it cannot be supplied to the market. In this time of the day, the minimum amount of nitrate is accumulated in leafy vegetables, with a gradual increase throughout the night. On the other hand, its highest level is in the early hours in the morning. The purpose of this study is to evaluate nitrate and nitrite accumulation in vegetables produced in different areas of Ahwaz during winter and spring. Materials and Methods This experiment was conducted in a randomized complete block design arranged in a split-split-plot design with three replications in the Department of Horticulture, College of Agriculture, Shahid Chamran University of Ahvaz. In the treatments viz. first to third harvest dates (1): February 6, 2016, (2):March 4, 2016 and (3): April 7, 2016 respectively were the main factors and nine vegetable species (including coriander, parsley, mint, fenugreek, chard, spinach, leeks, dill and watercress) kept in sub plot, three vegetable production areas in Ahvaz were arranged in sub plot in sub plots and three fields were selected in each of these areas. Before and after cultivation, all planting, harvesting and harvesting operations were carried out on the same field in the selected fields. The concentration of nitrate was measured by the method introduced by Cataldo et al. (1975) and the nitrite concentation was measured via the method of Abu-Dayeh (2009). The data were analyzed using SAS software and the mean comparison was calculated using Duncan's multiple range tests at a probability level of 5%. Results Nitrate and nitrite contents of the produced vegetables were different depending on various regions, harvesting time and vegetable species. The highest nitrate content was observed in parsley fromthe first harvest in the southern region of Ahwaz (4.4 mg/kg-1 dry matter) and the lowest amount of nitrate was recorded in watercress from the third harvest in the northern region of Ahvaz (1.4 mg/kg-1 dry matter). Also, the highest nitrite content was observed at the first harvest in the westhern region of Ahwaz in mint and the lowest amount of nitrate was recorded in the second harvest in the northern region of Ahvaz in chard. In winter, the environmental conditions in Ahwaz are suitable for the growth and development of leafy vegetables (Boroujerdnia et al., 2007); however, soil conditions provide manganese uptake by plants. Moreover, high light intensity is another factor which can decrease nitrate in leafy vegetables. The number of cloudy days in Ahwaz is very low; therefore, the number of sunny days with high radiation intensity is high in winter. This phenomenon greatly reduces the content of nitrate in leafy vegetables. Discussion The amounts of nitrate and nitrite content in various species of vegetables produced in three regions of Ahvaz during the cold season were much lower than the standard figures. Therefore, we can conclude that the quality of vegetables produced in Ahvaz is very high in terms of nitrite and nitrate contents.

Evaluation of Some Phytochemical Characteristics of Berberis integerrima in Response to Nano-Zinc Foliar Application and Post-Harvest Drying Temperature

lamia vojodimehrabani; Rana Valizadeh Kamran; mohammad bagher hassanpouraghdam

Volume 42, Issue 3 , December 2019, , Pages 345-358

https://doi.org/10.22055/ppd.2019.22929.1503

Abstract

Abstract Background and Objectives Mineral nutrition and post-harvest handling are the major factors affecting secondary metabolites content and composition of plants. Berberis integerrima ... Read More Abstract Background and Objectives Mineral nutrition and post-harvest handling are the major factors affecting secondary metabolites content and composition of plants. Berberis integerrima is one of the most important medicinal plants listed in all pharmacopeias. The bark, fruits and roots of this plant are commonly being used by traditional and modern medicine disciplines. They are well documented for their anti-diabetic, anti-cancer, and antimicrobial activities with berberine as the main active constituent. Also, the shrub has ornamental uses in the landscape and the fruit is used as a food additive. In addition, anthocyanin of the fruit is useful as a natural coloring agent in food industries. There are few studies on the agronomic demands and post-harvest handling of this plant. The experiment was conducted to assay the effects of nano-zinc foliar application and postharvest plant drying temperature on some phytochemical traits of this highly valuable plant. Materials and Methods The treatments were applied to assay the phytochemicals content variations in roots, fruits and aerial parts of the plant (2 years old) by two separate experiments as factorial based on RBCD. In the first experiment, the effects of nano-zinc foliar spray on the yield, anthocyanin content, Zinc content and TSS at the different mentioned organs were studied. In the second experiment, the effects of diverse post drying temperatures (25, 50 and 750C) on total phenolics content, flanonoids content, hydrolysable tannin and berberin content in Berberis integerrima were assayed. Results Zn content, TSS content and anthocyanins were affected by the independent effects of foliar spray and plant part. Plant dry weight was impacted by the organ type. Phenolics, flavonoids and alkaloids contents were affected by the interactional effects of the foliar spray , the organ type, and the drying temperature. The highest figure for the contents of alkaloids (30 mg g-1DWt) and flavonoids (26.9 mg g-1DWt) were from the root × foliar spray by 6 mgL-1 Nano-Zinc at 250C drying regime. The maximum amount of total phenolics was attained by the aerial parts × foliar spray by 6 mgL-1 Nano-Zinc at 250C and fruits × 3 and 6 mgL-1 foliar spray at 250C. For hydrolysable tannins (1.9%), the root and the aerial parts × 250C showed the largest figure. Discussion The overall results revealed the positive effects of nano-zinc foliar application on the studied gross secondary metabolites content in Berberis integerrima. The post-harvest drying temperature influenced the metabolites content as well, and the optimized temperature in the experiments was 250C.

Relationship of Color Indices with Some Chemical Characteristic in Red and White Apple Cultivars

Mitra Jabari; Mohammad Mehdi ُSHARIFANI; Ahad Yamchi

Volume 42, Issue 3 , December 2019, , Pages 359-372

https://doi.org/10.22055/ppd.2019.23071.1506

Abstract

Abstract Background and Objectives Apple is one of the most important common fruit trees in temperate regions of the world with a lot of phenotypes and genotypes. Fruit surface ... Read More Abstract Background and Objectives Apple is one of the most important common fruit trees in temperate regions of the world with a lot of phenotypes and genotypes. Fruit surface color is one of the important factors in evaluating the qualities and appearance of fruits. This is the first component of acceptance by the customer, which indicates anomalies in agricultural products. Materials and Methods For this purpose, a study was conducted in 2016 in a completely randomized design with 12 varieties of native and imported apples in three replications. In this research, qualitative characteristics including anthocyanins for skin and flesh fruit, total sugar, flavor index, pH and EC and color indexes including brightness index (L), redness index (a), yellowness index (b), Hue (H), and Chroma (C) were measured for each apple cultivar. Results The results showed the highest indexes of a and C of skin. Moreover, the index of a related to the flesh of fruit in Mazand 2 had the highest levels of Hue skin, brightness and Hue flesh in Gala cultivar. The highest amount of brightness and yellowneass of fruit skin and flesh was observed in Golden delicious cultivar. While the highest amount of anthocyanin in skin was found in Red delicious cultivar. the highest amount of anthocyanin in flesh was found in Mazand 2. Furthermore, the highest level of flavor index was found in Shahrod 10 and the maximum amount of total sugar was detected in Red delicious cultivar. The utmost EC juice and the highest juice pH were observed in Gol Ghermez and Golab Kohanz cultivars, respectively. The highest positive correlation was found between anthocyanin of skin and anthocyanin of fruit pulp in the red flesh apple (0.99). Based on the results of this research, the red index and the fruit flavor index correlated with skin Hue, brightness of skin correlated with yellow color of skin, and fruit EC correlated with sugar content of the fruit. Discussion Finally, the highest correlation was found between anthocyanin in skin and the red flesh apple. In other apple cultivars, a significant correlation was found between index (a) and the yellowness of the apples (0.92). Further positive correlation was set between index C and anthocyanin content and flavor index. A positive correlation was observed between H and L indexes and the yellow color of the apple. Index b indicated a significant negative correlation with anthocyanin of skin and flesh of apple. The highest red content in red flesh apples indicated positive correlation with a and c indices, which has already been confirmed by the results of other researchers.

Effects of Aloe vera Gel and Storage Duration on Some Biochemical Indices in Minimally Processed Pomegranate Fresh Arils

Madineh Taheri; Mokhtar Heidari; Mehdi Zarei

Volume 42, Issue 4 , December 2019, , Pages 495-508

https://doi.org/10.22055/ppd.2019.25498.1591

Abstract

Abstract Background and Objectives The effect of different physical and chemical treatments on quality of pomegranate fruit at postharvest has been studied, but little information ... Read More Abstract Background and Objectives The effect of different physical and chemical treatments on quality of pomegranate fruit at postharvest has been studied, but little information is available in the case of pomegranate fresh aril postharvest as a ready-to-eat product. Coating is one of the treatments to preserve the postharvest quality of the pomegranate fresh aril. Since consumer health is impotant in the selection of postharvest treatment, the use of compounds extracted from plants as wax or coating agent is a safe treatment for fresh arils. Extracted gel from leaf tissue of Aloe vera L. is an herbal gel that can be used as coating agent. The objective of the present experiment was to evaluate the effects of Aloe vera gel on the postharvest quality of pomegranate fresh arils. Materials and Methods Pomegranate Fruits (known as ‘Malas Poost Zard’) were harvested from a commercial orchard located in Baghmalek, Khuzestan. Fresh arils were removed manually from fruits and treated with Aloe vera gel (0, 2.5, 5 and 10%). Fresh arils were stored at 7 oC, RH = 90% for 4, 8 and 12 days. Some biochemical characteristics including ascorbic acid, total soluble solids, titratable acidity, total phenolics, color intensity, anthocyanins and browning index were evaluated. Results Results showed that the interaction of Aloe vera gel concentration and storage duration had a significant effect on ascorbic acid, titratable acidity, total phenolics, color intensity, anthocyanins and browning index. All concentrations of Aloe vera gel decreased total soluble solids. The highest concentration of Aloe vera gel (10%) significantly increased anthocyanins/browning ratio. The quality of pomegranate fresh arils significantly decreased with the increase in the duration of storage. During storage condition of fresh arils, Aloe vera gel (2.5 – 5%) improved the color intensity of fresh arils after 4 days. Also, results showed that all concentrations of Aloe vera gel helped to preserve higher ascorbic acid after 8 and 12 days. Discussion Aloe vera gel improved the quality of pomegranate fresh arils. Aloe vera gel contains the different antioxidant and nutrients which may be responsible for the positive effects on quality of pomegarnate arils. Results showed that Aloe vera gel is a promising herbal product for coating pomegranate fresh arils. More research needs to be undertaken on the efficacy of Aloe vera gel on postharvest quality of pomegranate fresh arils and other minimally processed products.

The Study of Changes in Phytochemical Compounds of Date Fruit cv. Barhee During Development and Ripening

Solaleh Najafi Marghmaleki; Mohamadhasan Mortazavi; Hossein Motamedi

Volume 42, Issue 4 , December 2019, , Pages 563-574

https://doi.org/10.22055/ppd.2019.26170.1611

Abstract

Abstract Background and Objectives Studying phytochemical changes of fruits is very important for plant physiologists and pomologists. Date is an important horticultural fruit ... Read More Abstract Background and Objectives Studying phytochemical changes of fruits is very important for plant physiologists and pomologists. Date is an important horticultural fruit in Iran mainly produced in southern regions. Barhee is considered one of the most important date palm cultivars. The fruit of cv. Barhee can be harvested and consumed at different maturation stages including Khalal, Rutab and Tamar. In the present study, the biochemical alterations of date fruit cv. Barhee during development and ripening stages were studied. Materials and Methods The experiment was carried out in a randomized complete block design with three replications. Sampling was done at Date Palm Research Institute Collection Orchard located in Ahvaz. First, fruits were harvested in five different developmental stages including late Kimri, Khalal, mainly Khalal, mainly Rutab, fully Rutab and Tamar. The fruits were then transferred to Quality Analysis Lab of Department of Horticultural Science at Shahid Chamran University of Ahvaz. Selected uniform fruits were used for the analysis of different phytochemical compounds. Chlorophyll, carotenoid, starch, soluble sugars, phenolic compounds, flavonoids and antioxidant capacity were analysed using spectrophotometric methods. The type and concentration of sugars, organic acids, and phenolics were assessed using HPLC. Also, vitamin C content was determined by titration againt DCIP dye. Results The results showed that soluble sugars increased significantly during ripening stages, and glucose and fructose were the main detected sugars for Barhee cultivar. The amount of sucrose was negligible. The acidity of the fruit increased at initial growth stages and then decreased gradually till Rutab stage. At the end of ripening process and at Tamar stage, the amount of organic acid content increased to 2.45 mg/g FW. Among the detected organic acids, acetic acid was dominant in Tamar stage, while in the earlier stages malic acid was considered the main organic acid. Other major investigated compounds, including flavonoids, chlorophylls, carotenoids, starch and vitamin C decreased during the development. Different compounds including p-cinamic, gintistic, gallic, chlorogenic, vanilic, caffeic, syringic and p-cumaric acids were detected by HPLC evaluation of phenolic acids. Among them, gallic acid was the dominant, and the trend was incremental during the development of fruit. Discussion Comparing the current results with published data for other date cultivars shows that there are considerable differences in biochemical compounds among different cultivars. In this cultivar, the increasing trend of accumulation of soluble sugars, organic acids, phenolic compounds and antioxidant capacity during ripening stages from late Kimri to Rutab and Tamar stage makes the fruit an important source of essential nutrients.

Effect of UV-C radiation on alleviating chilling injuries of persimmon fruit cv. Kashan during cold storage

Zahra Sadat Asgareyan; Mohammad Sayyari; Mahmood Asnaashari

Volume 42, Issue 2 , June 2019, , Pages 181-194

https://doi.org/10.22055/ppd.2019.18805.1367

Abstract

Effect of UV-C radiation on alleviating chilling injuries of persimmon fruit cv. Kashan during cold storageAbstractBackground and objectivesWith increasing interest for developing the ... Read More Effect of UV-C radiation on alleviating chilling injuries of persimmon fruit cv. Kashan during cold storageAbstractBackground and objectivesWith increasing interest for developing the cultivation of persimmon, there is not suitable technology and enough information about postharvest behavior and storability of this fruit in many of countries such as Iran. Softening, chilling injury and the incidence of different diseases are limiting factors for postharvest life of persimmons fruits during cold storage. Ultraviolet-C (UV-C) light radiation has recently suggested to controlling decay and reducing chilling injury of some fruits. Therefore, this study was conducted to evaluate the effects of UV-C treatments with different doses on alleviating chilling injuries and maintaining qualitative attributes of persimmon fruits during storage.Materials and methodsThe persimmon fruits cv. `Kashan´ were harvested from a commercial orchard near Kashan city. UV-C treatments was carried out at 0 (as control), 5 minutes irradiation from 15 and 30 cm distance with 3.2 and 0.8 kJm-2, and 10 minute irradiation from 15 and 30 cm distance with 16.4 and 4.1 kJm-2. After 1, 2, 3 and 4 months, fruits were removed from storage and chilling injury indices, polyphenol oxidase (PPO) activity and some quality attributes were measured. ResultsThe effect of UV-C treatments on weight loss, chilling index, electrolyte leakage, lipid peroxidation and PPO activity was effective during storage. High percent of weight loss (6.84) and chilling symptom (40) was observed in irradiated fruits with 16.43 kJm-2 and low level of mentioned parameters was seen in 3.2 kJm-2 treatment. High level of electrolyte leakage (80.49) and lipid peroxidation (1.78 nM g-1 FW) were detected in treated fruits with 4.1 and 16.43 kJm-2 and low level of above parameters were observed in non-treated and treated fruits with 3.2 kJm-2. Application of UV-C with high dose increased PPO activity (9.7 u gr-1 FW) in comparison to other treatments during storage. DiscussionOur results suggest that application of UV-C irradiation in low level after harvest could increase postharvest life of `Kashan´ persimmon by controlling chilling injuries and preserving fruit quality. However, it is worth mentioning that chilling symptoms of persimmon was reduced by low doses of UV –C irradiation. In fact, UV-C treatment with high doses caused high percentage of injured fruits. In conclusion, the use of UV-C radiation in controlling chilling injuries and maintaining quality of persimmons fruits offers an interesting research area and considering the low risk of the treatments.

Efficacy of some calcium salts foliar spray on growth and biochemical parameters of jujube fruit (Ziziphus jujuba Mill.)

M. Ghesmati; F. Moradinezhad; M. Khayat

Volume 41, Issue 3 , December 2018, , Pages 25-36

https://doi.org/10.22055/ppd.2017.21173.1447

Abstract

Abstract Background and Objectives South Khorasan province is the most important area of jujube fruit production in Iran. Fresh jujube fruit destroys in a short time at ambient temperature. ... Read More Abstract Background and Objectives South Khorasan province is the most important area of jujube fruit production in Iran. Fresh jujube fruit destroys in a short time at ambient temperature. Normally about one week after harvesting, a considerable loss occurs in picked fruits. Plant nutrition, during growth and developmental stages influence the quality of many fruits. Calcium is one of the most important elements that can affect quantity, quality, storability and market of many horticultural crops. Although South Khorasan province is located in arid areas, it has lime soils with enough calcium for plant growth and development. However, due to low mobility of calcium from soil to plant, calcium deficiency is common in horticultural plants. Thus, pre-harvest spray with minerals like calcium salts can be useful for reducing postharvest losses during handling and storage. Therefore, the aim of this research was to evaluate the effect of different calcium salts spray on physicochemical properties and quality of fresh jujube fruit. Materials and Methods Twenty jujube trees, eight years old, were sprayed with calcium salts twice, mid of June and early in July 2016 in a commercial orchard in Birjand, South Khorasan province. Experimental treatments were distilled water (control), calcium chloride (0.5 and 1%) and calcium nitrate (0.5 and 1%). Sprays were done at two stages of fruit growth and development, cell division (3 mm fruit diameter), and 20 days later in full cell enlargement stage. Fresh fruit was harvested at a crisp mature (white-red) stage early in August. The experiment was conducted in a completely randomised block design with four replications, and each replicate included 500 g of fresh fruit. Data were analysed using SAS program (ver. 8) and comparison of means was made with LSD test at 1 and 5% probability. Results Physiochemical properties of harvested fruit including fresh fruit weight, dry matter, fruit length and diameter, firmness, total soluble solids, pH, carotenoids, total phenol, and calcium content were evaluated. The results showed that calcium nitrate application at 0.5% significantly increased fruit fresh weight, dry matter and fruit length and diameter compared to control. Also, fruits that were sprayed with calcium chloride at 0.5% concentration had higher firmness, carotenoids and total phenol. The highest total soluble solids and calcium content values were obtained in treated fruit with calcium chloride (1%). Although different calcium salts spray had no significant effect on juice pH. Discussion In general, it can be concluded that physical fruit parameters were affected by calcium nitrate, while calcium chloride spray had a greater impact on chemical and nutritional quality aspects of fruit. Although calcium chloride at 0.5% was more effective on chemical factors, however, it was not significantly different with calcium chloride 1% treatment. Among different treatments, calcium nitrate at 0.5% had the highest effect on physical properties of jujube fruit. Therefore, it can be concluded that spraying of jujube fruit trees during fruit growth and developmental stages with both calcium salts at 0.5% concentration have beneficial effects as they improve the most important physical and chemical parameters of fresh jujube fruit.

Comparison of the Effect of Mint Extract and Chlorpropham on Preventing Potato Sprouting in the Storage

F. Goudarzi; R. Kalvandi

Volume 41, Issue 3 , December 2018, , Pages 51-62

https://doi.org/10.22055/ppd.2018.21602.1464

Abstract

Abstract Background and Objectives Sprouting is one of the main reasons of wasting about one half of potatoes during the storage. The existing methods to control sprouting in long term ... Read More Abstract Background and Objectives Sprouting is one of the main reasons of wasting about one half of potatoes during the storage. The existing methods to control sprouting in long term storage (for example postharvest applications of chlorpropham) can do the job as well, but the incidence of quality problems and undesirable side effects in such potatoes, and more importantly, the growing interest in organic food products, have challenged the application of these methods. Materials and Methods The peppermint extracts used in the experiment were obtained from dried peppermint leaves in 48 hours, at room temperature using 1:1 distilled water-alcohol solution, and with the normal air pressure. The peppermint extracts were dried for 10 days and then transferred to a dark glass bottle by means of petroleum ether (as solvent) and were kept at 2°C until being used for later use. Herbal extracts of peppermint were used at concentrations of 0, 0.5, 1, 2 and 4 g/l, with regular intervals of 2, 4 or 6 weeks during the 5 months of potato (Agria cultivar) storage while sprouting was monitored. Direct contact between tubers and the peppermint extracts was not allowed. To provide the needed oxygen for the tubers respiration, the containers apertures were opened for 15-20 minutes every four weeks and were sealed again. A group of tubers was only treated by 37g chlorpropham (5% purity). To do this, the powder was poured into the container, completely sealed and slowly shaken for 10 minutes. The sealed container was kept at room temperature for 48 hours. All experiments were repeated 3 times. Data was analyzed by a completely randomized design (CRD) with a factorial experiment. Results According to the results, by increasing the concentration of mint from 0.5 to 2 g/l, sprouting potato was controlled more effectively and meaningfully. More concentration in the control of potato sprouting was not significant. Use of this extract at a concentration of 0.5 g/l stimulates the germination of tubers compared to the control. Extensive sprouting of tubers was delayed for up to 4 months compared to the control treatment too. Application of peppermint extract caused %92 and %96 decrease in potato germination and total weight sprouting respectively. Organoleptic evaluation of the boiled and fried potato samples, treated with the mint extract and chlorpropham, did not show significant differences. Discussion Use of 2 g/l mint extract will be able to decrease waste potato tubers in storage up to 6 percent. Replication of every 4 weeks extracts application is absolutely essential for the best result. Organoleptic evaluation of the boiled and fried potato samples, treated with the mint extract and chlorpropham, received more points compared to control.

Effects of Satureja hortensis L., Thymus vulgaris L., Citrus limon L. Essential Oils and 8-Hydroxyquinoline citrate in Dry and Wet Storage Conditions on Vase Life of Cut Lily (Longiflorum × Asiatic Hybrids cv. CebDazzle) Flowers

N. Farhodi; M.R. Salehi Salmi; F. Yari; E. Shahbazi

Volume 41, Issue 2 , September 2018, , Pages 41-54

https://doi.org/10.22055/ppd.2018.19898.1414

Abstract

Abstract Background and Objectives Recently, the use of cut flowers has increased, and some of these cut flowers (Lily, Rose etc.) are among the most used for decorative purposes. With ... Read More Abstract Background and Objectives Recently, the use of cut flowers has increased, and some of these cut flowers (Lily, Rose etc.) are among the most used for decorative purposes. With this boom in popularity has also come a rise in the need for commercial use, and very little academic literature exists on how to best utilize the preservative solutions (here 8-HQC and essential oils) to prolong cut flowers vase life keeping their full quality. The vase life of Lily is usually short due to vascular occlusion. The use of synthetic chemical fungicides to protect cut flowers raises environmental and human health concerns because of chemical residue routinely found on food meant for consumption. Thus, biocides showing insignificant toxicity to human and organisms are more desirable and less likely to cause negative environmental effects. Materials and Methods The chemical composition of essential oils extracted from the endemic Iranian plants Satureja hortensis L., Thymus vulgaris L. and Citrus limon L. was studied in order to evaluate their efficacy as a substitute for synthetic chemical that was used as preservative-solutions. The effects of essential oils (250 mg L-1) individually and 8-hydroxyquinoline citrate (8-HQC) with sucrose 3% were evaluated. Cut flowers were pulsed for 12 h in different preservative solutions, followed by storage in dry and wet conditions (in distilled water) at 8˚C for 15 days, and then all treated cut flowers were kept in room temperature to evaluate commercial vase life. At the end of commercial vase life, parameters such as relative fresh weight, amount of chlorophyll, phenol, anthocyanin, protein and carbohydrate, vase life and antioxidant enzyme activities were measured. In this experiment, flowering stems were arranged using a completely randomized design. Results Limonene was identified as major component of C. limon L. essential oil, whereas Carvacrol was the major component in S. hortensis L. and Th. vulgaris L. Results showed that applying all essential oil treatments or 8-HQC increased positive characteristics and vase life compared with control. However, among all these treatments, the flowers treated with Thyme essential oil plus 3% sucrose or 8-HQC were kept in wet storage condition showed best vase life. Based on our results, new antimicrobial agents such as limonene, thymol and carvacrol in combination with 3% sucrose had a positive effect on the vase-life and relative fresh weight. The senescence of cut flowers is associated with a series of highly regulated physiological and biochemical processes, including breakage of water balance, degradation of photosynthetic pigment, decrease in metabolic constituents, and loss of membrane integrity. In our experiment, Thyme essential and 8-HQC oil treatments were reduced degradation of leaf protein and chlorophyll content which was seen in the controls. Essential oils derived from plants such as T. vulgaris are particularly valuable because of their antibacterial and antioxidant properties. Discussion In conclusion, using various concentrations of essential oils and 8-HQC in preservative solutions showed promising prospects for the utilization of natural essential oils or plant extracts in extending lily vase-life. The proposed mechanism of action, e.g. inhibition of the growth of bacteria in the vase water or inside the xylem vessels of the flower stem needs further elucidation.

The Effect of Planting Methods and Calcium Nanoparticles Spray on Quality, Quantity and Vase Life of Gladiolus hybrida cv. Magma

M. Chehrazi; D. Pourghasemi; M. Khoshbakht

Volume 41, Issue 2 , September 2018, , Pages 55-66

https://doi.org/10.22055/ppd.2017.20176.1418

Abstract

Abstract Background and Objectives In order to introduce the best method of planting between Cretan and furrow and also spraying nanoparticles of calcium on the quality and quantity ... Read More Abstract Background and Objectives In order to introduce the best method of planting between Cretan and furrow and also spraying nanoparticles of calcium on the quality and quantity of Gladiolus flowers (Gladiolus hybrida cv. Magma), a study was conducted at the research farm. Nanoparticles release nutrients slowly and continuously because of having nanomembranes so that we can adjust the speed of releasing nutrients from the capsuls. Using this fact, the efficiency of nanoparticles would improve. Cretan method is the most common method of planting of Gladiolus in Iran. Materials and Methods Corms were planted in two ways; Cretan and furrow planting. The density of them was 24 per square meter (four rows with the distance of 15 cm). Nanoparticles of calcium at three levels (0, 1 mg/litre, and 2 mg/litre) were sprayed on the plants before the harvest once a week for three weeks. Considering the importance of planting methods and performance of nanoparticles of calcium, a factorial experiment was conducted based on randomized complete block design with three replications. Data were analyzed using SAS software. Results In this study after the harvesting, most of the features of flowers were measured including: the inflorescence length, the inflorescence weight, the weight index of cut flower (fresh weight of the cut flower/ the length of the cut flower), the shoot diameter, the shoot length, the diameter of the first and the second floret, the number of florets, the number of corm, the corm weight, a, b, and the total chlorophyll, the relative water content, and the vase life. Significant differences were observed at the 5% level for all of the traits except the weight index of the cut flower and the relative water content. The cut flower weight, the cut flower length, the inflorescence length, the florets diameter, the number of florets, the number of corm, and the vase life were increased in the furrow planting method. The importance of the nanoparticles of calcium treatments on vase life was significant. The Number of corms was also significant in the furrow planting method. Discussion The results of this study showed that the quality, the yield and the vase life in furrow planting were better than those in the Cretan. The foliar feeding calcium nanoparticles resulted in survival improvement in post-harvest flowers. In Khuzestan, which is capable of producing commercial Gladiolus flowers in autumn and winter, the furrow planting can be used to obtain flowers with higher quality and quantity instead of using the cretan planting.

Effect of postharvest treatment of gamma-amino butyric acid on some biochemical and antioxidant properties of sweet cherry cv. Tak Daneyeh Mashhad

H. Hassanpour; A. Bisti; S. Nojavan

Volume 41, Issue 2 , September 2018, , Pages 67-78

https://doi.org/10.22055/ppd.2018.20300.1422

Abstract

Abstract Background and Objectives Sweet cherry fruit due to high water content and respiration rate is rotten in post-harvest period and hence has a short storage life. So, reducing ... Read More Abstract Background and Objectives Sweet cherry fruit due to high water content and respiration rate is rotten in post-harvest period and hence has a short storage life. So, reducing of respiration rate and delaying of senescence process are required for increasing of storage life. Gamma amino butyric acid (GABA) is a non-protein amino acid with low content in the cell in normal state. No study has taken into account the effect of GABA on the storage life of sweet cherry fruit and also, increasing of shelf life by natural compounds seems essential to maintain the quality. Therefore, this study was conducted with the aim of improving the post-harvest quality of sweet cherry fruit, increasing biochemical and antioxidant properties and improving fruit appearance using GABA with the purpose of replacing it with synthetic chemicals. Materials and Methods Fruits of sweet cherry cv. Tak Daneyeh Mashhad were harvested at commercial maturity from a commercial orchard in Urmia (Iran) and transported to laboratory immediately. GABA was used in three concentrations (5, 10 and 20 mM). Titration method was used for determination of Titratable acid (TA) and pH was determined by pH meter. TSS was determined by refractometer. Total antioxidant capacity was determined by ferric ions reducing antioxidant power assay (FRAP). Total phenolic content was determined by Folin ciocalteu method and total flavonoid content was determined by the aluminum chloride colorimetric method. Results The results showed that TA content remains constant with treatment of GABA during storage. GABA treatment leads to an increase in total antioxidant activity, total phenol, total flavonoid and activity of catalase and guaiacol peroxidase enzymes in the treated fruits compared to control fruit after 30 days of storage. The pH value in treated fruits compared to untreated fruits was decreased during storage. Also, GABA at 10 mM level compared with other concentrations had the highest effect on total antioxidant capacity after 30 days of storage. Discussion The increasing of organic acids in treating fruits can be probably due to increasing of antioxidant capacity by GABA treatment, hence the damage to membrane was prevented and the organic acids were used less frequently. Treatment of fruits with GABA decreased the pH during storage, which may be due to its role in reducing of respiration rate in harvested fruits. The mechanism of the effect of GABA on increasing of total phenol and flavonoid content may be due to stimulating the production of Phenylalanine ammonia lyase (PAL) which can trigger phenylpropanoid pathway and hereby, syntheses of phenolic compounds such as flavonoids compounds were occurred. Catalase is one of the key enzymes to protect the cells and cause the conversion of hydrogen peroxide to water and oxygen. Therefore, probably the GABA treatment via the increasing the antioxidant system such as catalase leads to scavenge free radicals thereby protecting plants against oxidative stress.

The Growth and Physiological Characteristics of Three Commercial Rootstocks of Pistachio (Pistacia vera L.) Under Controlled Temperature Conditions

H. Hokmabadi; M. Rezaei; H. Hokmabadi

Volume 41, Issue 2 , September 2018, , Pages 79-90

https://doi.org/10.22055/ppd.2018.20314.1423

Abstract

Abstract Background and Objectives In recent decades, global warming causes climate changes which triggers physiological and nutrition disorders in plants. Iran is a main pistachio ... Read More Abstract Background and Objectives In recent decades, global warming causes climate changes which triggers physiological and nutrition disorders in plants. Iran is a main pistachio producer in the world. Pistachio cultivar is mainly grafted into seedling rootstocks are which obtained from especial cultivars and wild type pistachio. The changes in the vegetative growth and yield in pistachio orchards have been obviously observed in the years with so warm or so cold weather conditions. The purpose of this research was evaluating the effects of temperature on the growth of three pistachio rootstocks. Materials and Methods In order to study the effect of temperature on the growth and physiological characteristics of three commercial pistachio rootstocks, a factorial experiment on the basis of a completely randomized design (CRD) was carried out with three replications. The first factor was three rootstocks including: Pistacia vera cv. Badamie-zarand, P. vera cv. Ghazvini and P. vera var. Sarakhsi and the second factor was four temperature conditions including: 10, 15, 20 and 25 degrees Celsius. Pistachio seedlings after planting up to 15-leaf stage were kept in the greenhouse condition and then the pots were transferred to growth chambers which adjusted to defined temperature treatments. The plants were kept in temperature treatments for fifteen days. At the end of experiment, growth parameters including fresh and dry leaf weight, fresh and dry weight in shoot and root and leaf area and physiological parameters including stomatal conductance, relative water content of leaves and leaf chlorophyll index were measured. The statistical analysis was done using SAS 9.1 software and mean comparison was done by using the lowest significant difference test (LSD). Results The results showed that with increasing environmental temperature, an increase in the number of leaves, stem height, dry weight of leaf, stem and root, leaf area was observed in pistachio rootstocks. There were significant differences among rootstocks in most of the growth parameters. Badamie-Zarand was more vigorous than the other rootstocks in low temperature. The highest SPAD index was observed in high temperature environment (20, 25°C) beside lowest SPAD index level was observed at Low temperature environmental (10, 15°C). Minimum and maximum stomatal conductance was respectively obtained at 10 and 25 °C in Sarakhsi rootstocks which reveals the sensitivity of this rootstock to temperature fluctuations. In contrast, stomatal conductance variations in the Ghazvini rootstocks are lower than the other rootstocks when exposed to different environmental temperature. This may show the tolerance of this variety to changes of environmental temperature conditions. The leaf RWC in three pistachio rootstocks at different ambient temperatures shows a significant difference. At 15 and 20°C, Ghazvini rootstock has the highest RWC and Badamie- Zarand rootstock has the lowest levels of RWC. The highest relative water content was obtained at 10°C in Sarakhsi rootstock and at 25°C in Badamie-Zarand rootstock. Positive correlation between root dry weight and stomatal conductance reflects the rootstock compatibility with ecological conditions. Discussion Badami Zarand rootstock has more vegetative growth in low temperature environment and more adaptation to a wide range of temperature in comporison to two others rootstock and also can be recommended to nursery as a rootstock for planting.

The Effect of Salinity and Fruit Ripening Stage on Some Quantitative and Quantitative Characteristics of Tomato in Hydroponics

R. Azarmi; N. Chaparzadeh

Volume 41, Issue 2 , September 2018, , Pages 91-102

https://doi.org/10.22055/ppd.2018.20715.1432

Abstract

Abstract Background and Objectives Tomato is one of the most important vegetable in Iran, not only because of its economics importance, but also for the nutritional value of its fruit, ... Read More Abstract Background and Objectives Tomato is one of the most important vegetable in Iran, not only because of its economics importance, but also for the nutritional value of its fruit, mainly due to the fact that they are an excellent source of natural colors and antioxidant compounds. Water quality and fruit maturity stage has a significant effect on tomato fruit quality. In the present research, we studied the response of tomato grown at different salinity levels, as well as the changes that take place during different maturity stages, in order to improve the management and harvesting of tomato and obtain fruit of a higher nutritional value. Materials and Methods In this study, to evaluate the effect ofsalinity and fruit ripening stage on physical, biochemical properties and shelf life of tomato fruits in hydroponics system, an experiment was carried out as factorial based on randomized complete block design with three replications at Moghan College of Agriculture and Natural Resources, during 2015. Fruits from plants grown under three saline treatments (2, 4 and 8 dS m-1) were harvested at three ripening states (mature green, purple and red colors). The nutrient solution was prepared based on full strength of Hoagland's solution containing: 5.6 mM Ca (NO3)2, 4 mM KNO3, 1 mM KH2PO4. Characteristics such as fruit fresh and dry weight, total soluble solid, firmness, flavor index, titratable acidity, soluble protein, ascorbate peroxidase activity and shelf life were measured. Results The results showed that total soluble solids, juice electrical conductivity and dry matter percentage of fruits significantly increased with increasing salinity in the nutrient solution and progressed the ripening stage, but fruit water content decreased by 9%. The highest weight (147.7 g) and volume of fruits (153 cm3) were obtained in plants grown under 2 dS m-1 salinity and fruits harvested at the purple stage. Titratable acidity increased with increasing of salinity in the nutrient solution, but its content decreased at the red color stage. Firm fruit with greater shelf life was observed under 2 dS m-1 salinity and at the mature green stage. Soluble protein content in fruit was increased by increasing salinity levels in the nutrient solution, especially at the ripening stage. The application of 4 dS m-1 salinity at mature green stage increased ascorbate peroxidase activity up to 62.5% compared to 8 dS m-1 salinity and red color fruits. Discussion These results suggest that the improvement in fruit quality induced by the salinity is achieved by some biochemical changes and the reduction of fruit water content. Increasing of salinity in the nutrient solution can be useful for improving fruit quality, especially, when fruit is harvested at the red stage. However, with increasing salinity in the nutrient solution up to 4 dS m-1 and fruit harvest in the red color stage fruits with better quality and more suitable weight can be produced.

Identification of resistant Avena ludoviciana Dur Accessions to ACCase Inhibitor Herbicides in Gonbad-E Kavus Wheat Fields and Mapping Their Distribution

S. Tatari; J. Gherekhloo; A. Siahmarguee; H. Kazemi

Volume 41, Issue 2 , September 2018, , Pages 103-116

https://doi.org/10.22055/ppd.2017.21015.1439

Abstract

Abstract Background and Objectives Wheat is the most important crop in Iran and every year about 6.5 million ha of arable lands in the country is cultivated by this strategic crop. ... Read More Abstract Background and Objectives Wheat is the most important crop in Iran and every year about 6.5 million ha of arable lands in the country is cultivated by this strategic crop. Weeds cause 23 to 30% reduction in yield of wheat annually. Winter wild oat is the most serious grass weed of wheat fields of Iran. Chemical method is the common way to weed control in wheat fields. ACCase inhibitors such as clodinafop-propargyl, diclofop-methyl and Fenoxaprop-P ethyl + Mefen pair di ethyl are widely used for control of grasses in this regard. Continuous application of herbicides with the same mode of action has resulted in developing herbicide resistant biotypes of weeds such as Avena ludoviciana. The objectives of the current study were: 1) to screen the collected accession of A. ludoviciana suspected to resistance to FOP herbicides, 2) to determine the resistance factor of resistant biotypes and 3) mapping distribution of resistant biotypes. Materials and Methods In order to survey Avena ludoviciana resistance to clodinafop-propargy, diclofop-methyl and Fenoxaprop-P ethyl+ Mefen pair di ethyl herbicides in wheat fields of Gonbad-e Kavus, several experiments were conducted in 2014 at Gorgan University of Agricultural Sciences and Natural Resources. (115 populations). The seed bioassay in Petri dish and pot experiment was conducted to determine resistance to acetyl coenzyme Acaboxylase inhibitors (ACCase), respectively. The seed bioassay experiment consisted of screening the suspected populations with discriminating concentration and concentration-response bioassay for resistant populations. Results In 48, 27 and 21 populations of A. ludoviciana were resistant to clodinafop-propargyl, diclofop-methyl and Fenoxaprop-P ethyl + Mefen pair di ethyl herbicides. Resistance accessions indicated varying degrees of resistance to tested herbicides. Based on EC50, the lowest and highest resistance to Clodinafop-propargyl herbicide was shown in Accessions of 74 and 6 with resistance factors of 1.99 and 31.42, respectively. Also for Diclofop- methyl herbicide, EC50 values from 2.88 for accession of 103 (with 1.34 resistanc factor) to 16.02 for accession of 91 (with 7.45 resistance factor) were different. In addition, EC50 estimatedfor Fenoxaprop-P ethyl + Mefen pair di ethyl herbicide was different from 0.124 to 0.86 for 20 and 6 accessions, respectively. Checking the distribution map of resistance accessions showed these accessions had uniform distribution in this city. Discussion Based on these observations, the existence of cross-resistance to herbicides was proved in some of the collected accessions. The results of this study can be used to correct programs designed to manage weeds herbicide resistance and prevent of the development of the plants in other areas.

Effect of Gamma-Amino-Butyric Acid Foliar Application on Physiological Characters of Tomato (cv. Namib) under Salinity Stress

L. Zarei; M. Koushesh Saba; Y. Vafaee; T. Javadi

Volume 41, Issue 1 , June 2018, , Pages 15-28

https://doi.org/10.22055/ppd.2018.13547

Abstract

Abstract Background and Objectives Salinity and salt accumulation in the soil surface is one of the most important abiotic stresses that limit agricultural crop production in ... Read More Abstract Background and Objectives Salinity and salt accumulation in the soil surface is one of the most important abiotic stresses that limit agricultural crop production in the arid and semi-arid regions of Iran. Thus, finding an alternative production technique or materials to alleviate stress condition is a research priority. One way to decrease the harmful effects of salinity is the foliar application of some chemicals such as Gamma-amino butyric acid (GABA) to increase plant tolerance to salinity. GABA is often accumulated in plants in response to live and non-live stresses such as drought, salinity, oxygen deficit, heat shocks and contamination of pathogens. Materials and Methods The current study was carried out to monitor the GABA effects on tomato physiological changes under salinity stress. The plants were grown in hydroponic system and received 0 and 50 mM NaCl as control and salinity stress, respectively. GABA at three concentrations 0, 10 and 20 mM was applied as a foliar application in either control or salinity treatments. Physiological characteristics of relative water content (RWC), membrane stability index (MSI), proline, total soluble protein (TSP) and enzymes activity, chlorophyll a, chlorophyll b, total chlorophyll and total soluble carbohydrates (TSC) were measured. Results The results showed that salinity stress and GABA were effective on the physiological characteristics of tomato. GABA can increase the tolerance of plants to environmental stresses, including salinity stress. MSI, chlorophyll a, total chlorophyll, carotenoids in salinity treatments were lower than control while TSC, proline, peroxidase and SOD increased. RWC, MSI, proline, TSC of GABA treated plant were greater than untreated in either salinity or control conditions. Also, SOD and peroxidase activity elevated in GABA treated plant under salinity stress. Discussion GABA increased antioxidant capacity in the plant and thus might eliminate radicals and prevents the destruction of cell membrane tissue, including the chloroplast membrane. In general, the results showed that in salinity stress conditions, GABA application as a compatible osmolytes might improve the tomatoes physiological performance and alleviate salinity stress.

Effects of Harvest Time and Storage Duration on Some Physical and Biochemical Indices in Pomegranate (Punica granatum L.) Fruits

F. Paimard; M. Heidari

Volume 41, Issue 1 , June 2018, , Pages 57-68

https://doi.org/10.22055/ppd.2018.13550

Abstract

Abstract Background and Objectives Preharvest and harvest conditions can affect quality and biochemical composition of fruit during storage. Pomegranate (Punica granatum L.) is a non-climactric ... Read More Abstract Background and Objectives Preharvest and harvest conditions can affect quality and biochemical composition of fruit during storage. Pomegranate (Punica granatum L.) is a non-climactric fruit and there is no clear physiological index to determine the proper harvest time. Lack of standard maturity index results in prolonged period of picking pomegranate fruit within the season. The effects of different physical or chemical treatments on postharvest quality of pomegranate fruit have been investigated in many studies, but our knowledge on the pre-harvest and harvest aspects that influence pomegranate fruit postharvest physiology is scarce. The objective of the present experiment was to determine how qualitative indices of pomegranate fruit in ‘Bagmalek’ (Khuzestan, southwest of Iran) are affected by maturity stages at harvest and storage duration. Materials and Methods A factorial design including three harvesting times and three storage durations with three replications was used for statistical analysis. Fruits of ‘Malas Post Zard’ as an important pomegranate cultivar were harvested from a commercial orchard in ‘Bagmalek’ (150 Km Ahwaz, Khuzestan, southwest of Iran). Fruits were harvested at three harvest times at 14-day intervals (14 September, 29 September and 13 October 2011) and stored for 40 and 80 days at +7 oC. Some physical (weight, surface color, decay and shriveling) and biochemical characteristics (ascorbic acid, total soluble solids, titratable acidity, pH, anthocyanin index and browning index) were evaluated. The factors were evaluated at harvest time and after 40 and 80 days. Results Regardless of storage durations, pomegranate fruit showed significant variations in total soluble solids, titratable acidity, pH and anthocyanins. Interactions of the harvest time and storage duration (for 40 and 80 days) were significant on gravity, surface color changes, shriveling, ascorbic, acid, pH, titratable acidity and fruit juice pigments (anthocyanin index and browning index). Results showed that the period 29 September until 13 October 2011 and storage duration for 40 days are the best treatments to maintaining postharvest quality of ‘Malas Post Zard’ fruits in ‘Bagmalek’ (Khuzestan, Iran). Discussion Results showed that quantity and quality indices of the pomegranate could be improved through the selection of proper harvesting time. Harvest of pomegranate fruit at different maturity stages altered fruit appearance quality indices and biochemical characteristics under ‘Bagmalek’ geographic condition, and at early harvest time (14 September), it reduced fruit quality but did not make its consumption unacceptable. More research needs to assess the effect of different climatic parameters such as temperature and light intensity on the postharvest quality of pomegranate fruits in Khuzestan.

Effects of Calcium Chloride Sprays to the Leaves and Fruits on Postharvest Physiological Characteristics of Papaya (Carica papaya L. cv. Eksotika II) Fruits

A. Mirshekari; B. Madani

Volume 40, Issue 3 , December 2017, , Pages 89-100

https://doi.org/10.22055/ppd.2017.17673.1335

Abstract

Abstract Background and Objectives Papaya (Carica papaya L.) is a tropical fruit crop. The ‘Eksotika II’ is high yielding cultivar with pleasant aroma, but fruits soften ... Read More Abstract Background and Objectives Papaya (Carica papaya L.) is a tropical fruit crop. The ‘Eksotika II’ is high yielding cultivar with pleasant aroma, but fruits soften quickly and lose their quality. Low calcium content causes low fruit quality and storage life. Foliar application of calcium on the fruit is significant to make the cell membrane integrity and cell wall firm and improve fruit quality. The objectives of this study were to determine the effects of calcium chloride foliar application on postharvest quality of papaya fruits. Materials and methods In order to evaluate the effect of calcium chloride (0, 0.5, 1, 1.5 and 2) on leaves, fruits and leaves + fruits of papaya, a factorial experiment based on RCBD was conducted in 2012. Calcium chloride was sprayed starting 21 days after flower anthesis and continued every two weeks for six times. Fruits were stored at 12±1 ̊C and RH=85-90% after harvest. The calcium and magnesium, respiration rate and ethylene, firmness, titratable acidity (TA), soluble solids concentration (SSC) and quality were determined 21 days after storage. Results The highest calcium content in fruits was observed with fruits and fruits + leaves sprayed treatments, while the lowest was recorded on leaves sprayed. Magnesium in peel and pulp of fruits that sprayed on fruits or fruits + leaves was lowest, while the highest was observed in leaves sprayed. Decreased in respiration rate and ethylene production were observed in fruits sprayed on fruits and fruits + leaves. The SSC was lowest in fruits + leaves treatment. Inverse results were recorded for TA. Moreover, calcium content in the peel and pulp, TA and overall quality increased when calcium chloride concentration in fruits and fruits + leaves treatments increased, While there was no effect on calcium content when applied to the leaves, but it increased TA. Discussion Results of this study showed that calcium could not transfer from leaf to fruit and vice versa in papaya. Since calcium moves in the transpiration stream, little or no subsequent translocation occurs from leaf to fruit. Unchanged ethylene production and respiration rate when calcium was applied to the leaves might be related to the immobility of calcium. Calcium was considered as a binding agent between cell walls which result in higher fruit firmness. Calcium is hypothesized to delay ripening by reducing disintegration of tissues and maintaining membrane integrity. The positive correlation might be related to magnesium and malate in fruits and malate might not be used in the process of respiration and thus increase in TA. The effect of calcium in decreasing SSC in fruits is possibly due to the decreasing respiration rate and metabolism activity that postpones ripening process.

Volume 46 (2023)

Volume 45 (2022)

Volume 44 (2021)

Volume 43 (2020)

Volume 42 (2019)

Volume 41 (2018)

Volume 40 (2017)

Volume 39 (2016)

Volume 38 (2015)

Volume 37 (2014)

Volume 36 (2013)

Volume 35 (2012)

Volume 34 (2011)

Volume 33 (2010)

Volume 32 (2009)

Main Subjects = H: Post Harvest Physiology

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