Simple sequence repeat (SSR) markers are valuable tools for many purposes, such as mapping, fingerprinting, and plant breeding. However, they are only available in some economically ...
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Simple sequence repeat (SSR) markers are valuable tools for many purposes, such as mapping, fingerprinting, and plant breeding. However, they are only available in some economically important crops because of the high cost and labor intensity involved in their development. Comparative mapping reveals a high degree of colinearity between closely related species and genera, which allows the exchange of markers between them. Our objective was to examine the transportability of sorghum SSR markers to sugarcane (Saccharum spp) and maize (Zea mays). Out of 29 sorghum SSR markers, 20.7% and 6.9% were amplified in sugarcane and maize, respectively. SSR markers were informative, even though only six varieties of each species were examined. The number of fragments amplified by the polymorphic primers ranged from 4 to 9 with an average of 6.2 and 2-3 with an average of 2.5 in sugarcane and maize, respectively. Fragments amplified revealed size variation in target species, as compared to the sorghum markers. One marker, amplified among three related species, suggests that these allelic regions of the primer binding sites are conserved. These markers with transportability between species can be used for breeding and genetics studies.
In the present study, 37 microsatellite primerpairs were used for germplasm analysis and estimation of the genetic relationship and diversity between 21 spring and winter genotypes ...
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In the present study, 37 microsatellite primerpairs were used for germplasm analysis and estimation of the genetic relationship and diversity between 21 spring and winter genotypes of Persian breed wheat. Genetic diversity (DI) and polymorphism information content (PIC) were calculated with average 0.68 and 0.63, respectively. Genetic similarity was calculated with Nei & Li and Jaccard methods with average 0.201 and 0.325, respetcively. The highest levels of genetic similarity for genotypes were found in Ghods and Alvand genotypes by two methods and the lowest was in Bolani and Niknejhad with Nei & Li and Jaccard methods.The number of alleles per microsatellite marker varied from 2 to 10 (average 5.37) and a total of 199 alleles were detected. The result showed all of the genotpyes could be distinguished and clustered into 4 groups with Nei & li similarity and UPGMA clustering method and all cultivars segregated by growgth type, i.e spring and winter. The data suggest that microsatellite can be used to estimate high and repeatable Polymorphism genetic diversity.
