Plant Productions

Rapid test for Detecting Haloxyfop-R methyl Ester Resistance in Winter Wild Oat (Avena ludoviciana durieu)

Saeid Hassanpour-bourkheili; Javid Gherekhloo; Behnam Kamkar; Seyed Sanaz Ramezanpour

Volume 45, Issue 2 , September 2022, , Pages 267-276

https://doi.org/10.22055/ppd.2021.33123.1887

Abstract

IntroductionDetection of resistance to an herbicide in a putatively resistant accession requires a series of experiments which are capable of illustrating the response of the accession ... Read More IntroductionDetection of resistance to an herbicide in a putatively resistant accession requires a series of experiments which are capable of illustrating the response of the accession to various herbicide doses. Whole plant bioassay in pots usually takes approx. 2 months to obtain the results, thus, rapid tests were developed to accelerate the process. Although determination of discriminating concentration as well as conduction of rapid test for some ACCase inhibitors has been performed by various researchers, no reports are available in this regard for haloxyfop-R methyl ester herbicide in winter wild oat. Thus, the following study was conducted with the objective of rapid detection of resistance to the mentioned herbicide in this weed using the rapid test. Materials and MethodsThe experiment was conducted using the seeds of 7 putatively resistant winter wild oat accessions and a susceptible biotype gathered from canola farms of Kalaleh Township located in Golestan province in 2015. Rapid test in petri dishes was conducted as a completely randomized design with three replications, with each petri dish as one replicate. To determine the discriminating concentration, various concentrations of haloxyfop-R methyl ester was applied on the susceptible accession and then, all putative accessions were screened using this concentration. The biotypes of the studied weed were exposed to various doses of the herbicide in the petri dish bioassay to determine the resistance factor. Also, a completely randomized design experiment with three replications was conducted for screening of putative accessions in the greenhouse. Accessions which maintained their survival and dry weight respectively 50 and 80 percent compared to the unsprayed control were selected. A whole plant dose-response bioassay was also done separately for each biotype. Results and DiscussionDiscriminating concentration of haloxyfop-R methyl ester for winter wild oat was obtained 0.106 mg ai. L-1. According to the results, 5 out of 7 accessions were detected as resistant and underwent the concentration- response assay in petri dishes. Resistant factors of the biotypes in the rapid test ranged from 2783.054 to 3421.414. However, no significant difference was observed among the biotypes. According to the results of the greenhouse, 5 accessions were detected as resistant with resistance factors of 14.19 to 18.54, with no significant difference among the biotypes. There was a positive and significant correlation between the results obtained from the rapid test with the greenhouse assay, which indicates the validity of the Petri dish assay. ConclusionThe results of the rapid test are in accordance with those of the whole plant assay in pots. Therefore, putative winter wild oat accessions collected from the region may be screened using the obtained concentration of haloxyfop-R methyl ester (0.106 mg ai. L-1) and resistant biotypes may be detected more rapidly compared to greenhouse assays. Due to the swift development of herbicide resistance issue, rapid detection of resistance is essential. Thus, using methods such as rapid test may be very feasible.

Effect of Permissible Inhibitors from Mixing of Several Medicinal Plants on Weed Seed Bank to a Depth of 20 cm Soil

vahid Chamani; Elnaz Farajzadeh-Memari-Tabrizi

Volume 44, Issue 1 , June 2021, , Pages 115-128

https://doi.org/10.22055/ppd.2020.28826.1748

Abstract

AbstractBackground and ObjectivesWeed seed bank in the soil is an essential source of weeds that enables them to be present in the fields. The seed bank density in a crop may reach ... Read More AbstractBackground and ObjectivesWeed seed bank in the soil is an essential source of weeds that enables them to be present in the fields. The seed bank density in a crop may reach a million seeds per square meter. Depending on the crop operation, crop rotation, and herbicide selection, the weed seed bank differs according to the location and time. Due to the economic benefits and improved crop ability, plant remains have become an attractive and acceptable way in agriculture. Allelopathic plant remnants can have a beneficial effect on weed control. The allelopathy of these plants plays a vital role in controlling the seed bank of weed soils. Therefore, this study was conducted to investigate the effect of mixing different amounts of residues of three medicinal herbs on the potential of the weed seed bank in the Malekan region. Materials and MethodsThis study was carried out at Malakan Azad University labs in 2018. Treatments included mixing different amounts of residues (0, 50, 100, and 150 g / m2), and three medicinal plants were cumin, seaweed, and marjoram. Samples had taken from a depth of 0 to 20 cm in an ocher system (7 cm radius) to measure the soil's seed bank. Weeds were isolated, and their species were identified. Laboratory studies were conducted to determine the germination strength of these seeds. This part of the experiment was carried out with three replications in a completely randomized design. ResultsIn total, 53915 weed seeds were detected per square meter. The highest seeds in the soil belonging to weed carnivorous and lambspeed were 33254 and 12457 seed per square meter of soil, respectively. The seeds of these two weeds included 95.4% of the seed bank of soil weeds. In this study, the remnants of seaweed and marigold caused a significant decrease in germination characteristics and the growth of seedlings of these weeds. Remains of cumin have little effect on the traits studied. Seed germination and seedlings growth of these weeds decreased by 44.7% and 84.9%, respectively. Discussion By increasing the amount of application of the remains, a more significant reduction in germination and weed growth was achieved. The remnants of the two herbs had a similar effect on germination and seedling growth. Overall, this study showed that the remnants of medicinal plants could reduce the diversity and abundance of seed banks of soil weeds.

The Effect of Gibberellic Acid on Vegetative Characteristics of Ber tree (Ziziphus spina-christi ) Seedlings

N M; M GH; M S

Volume 32, Issue 1 , September 2009, , Pages 81-88

Abstract

Application of gibberellic acid (GA3) increases the rate of vegetative growth of plants. This research was conducted to study the effect of gibberllic acid (0, 250, 500 and 750 mg l-1) ... Read More

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Keywords = Seedling

Rapid test for Detecting Haloxyfop-R methyl Ester Resistance in Winter Wild Oat (Avena ludoviciana durieu)

Abstract

Effect of Permissible Inhibitors from Mixing of Several Medicinal Plants on Weed Seed Bank to a Depth of 20 cm Soil

Abstract

The Effect of Gibberellic Acid on Vegetative Characteristics of Ber tree (Ziziphus spina-christi ) Seedlings

Abstract