Plant Productions

Phytochemical changes of Denak (Oliveria decumbens) in response to salinity stress and application of salicylic acid hormone

Anahita Boveiri Dehsheikh; Mohammad Mahmoodi Sourestani; Naeimeh Enayatzamir; Mehdi Safdarian

Volume 46, Issue 2 , September 2023, , Pages 225-236

https://doi.org/10.22055/ppd.2023.43201.2090

Abstract

Introduction Salinity stress is one of the most important problems in the cultivation and production of agricultural products, including medicinal plants. The expansion of saline lands ... Read More Introduction Salinity stress is one of the most important problems in the cultivation and production of agricultural products, including medicinal plants. The expansion of saline lands following continuous droughts in recent years increases the importance of the tolerance level of medicinal plants determination, their type of reaction, and the necessity of finding a solution to reduce the damage of mentioned stress on plants and increasing the productivity of the above lands. Materials and Methods To study the effect of salinity stress and salicylic acid hormone on biochemical traits of Denak (Oliveria decumbens), a factorial pot experiment was conducted as randomized complete block design with three replications in the research farm of Shahid Chamran University of Ahvaz in 2020-2021. The experiment factors included irrigation water salinity in four levels (1.1 (tap water), 2.5, 5, 7.5 dS/m) and foliar application of salicylic acid hormone in four levels (0, 100, 200, 500 mg/L). Biochemical traits included total soluble carbohydrates, proline, malondialdehyde, sodium and potassium elements, the activity of superoxide dismutase, catalase, guaiacol peroxidase, ascorbate peroxidase, polyphenol oxidase and phenylalanine ammonia lyase enzymes were measured at flowering stage. Results and Discussion The interaction effect of salinity stress and foliar application of salicylic acid hormone significantly changed all the traits of Denak. The foliar application of 200 mg/L of this regulator in plants under 5 dS/m salinity stress increased the amount of total soluble carbohydrates, proline, the activity of superoxide desmutase, catalase, guaiacol peroxidase, ascorbate peroxidase, polyphenol oxidase enzymes compared to the control treatment. The highest level of activity of ascorbate peroxidase and phenylalanine ammonialyase enzymes was also obtained in the foliar spraying of plants under 5 dS/m salinity stress by the concentration of 500 mg/L of salicylic acid hormone, but no significant difference was observed between this concentration and the application of 200 mg/L of this hormone. The maximum concentration of potassium element and the minimum amount of malondialdehyde and sodium element of Denak leaf were also recorded in the treatment of plants under 2.5 dS/m salinity stress by the concentration of 200 mg/L of salicylic acid hormone. The salicylic acid regulator as a very important messenger in the plant's defense system through increasing the stimulation of antioxidant enzymes in Denak reduced the negative effects of free radicals, membrane destruction and the production of malondialdehyde compound in a 2.5 dS/m salinity stress. On the other hand, the application of this hormone reduced the damage caused by the secondary stress of dehydration by increasing the ability of the plant to produce osmotic pressure regulating compounds including soluble carbohydrates and proline. Conclusion Due to its ability to increase the production of osmolyte compounds including soluble carbohydrates and proline, activation of antioxidant enzymes and maintaining the stability of cell membranes, Denak is able to withstand salinity stress up to the level of 5 dS/m. The application of 200 mg/L of salicylic acid hormone is beneficial and recommended due to its positive effect on antioxidant capacity and increasing the production of osmotic protective compounds in the plant as a solution to increase the resistance of Denak against the salinity stress.

Effect of waterlogging stress at the three-leaf stage on the growth and some physiological characteristics of bread wheat (Triticum aestivum L.)

Seyed Bashir Fazeli; Moosa Meskarbashee; Afrasyab Rahnama Ghahfarokhi

Volume 46, Issue 2 , September 2023, , Pages 279-292

https://doi.org/10.22055/ppd.2023.42792.2072

Abstract

IntroductionWheat is one of the most important crop plants in the world. Waterlogging stress causes serious damage to the wheat crop wheat crop worldwide annually, and it is likely ... Read More IntroductionWheat is one of the most important crop plants in the world. Waterlogging stress causes serious damage to the wheat crop wheat crop worldwide annually, and it is likely that these damages are increasing with climate change. In many cases, waterlogging stress occurs for various reasons in the initial stages of the growth of this plant. There are many reports about the existence of differences in waterlogging stress tolerance in different cultivars and genotypes of wheat, but so far these differences have not been fully exploited. This research was designed and carried out to investigate the effect of waterlogging stress on the dry matter yield of shoot system, the concentration of photosynthetic pigments, the activity level of antioxidant enzymes, the concentration of malondialdehyde and carotenoid content. Materials and MethodsFloods will increase as a result of climate change and in some areas will have a negative impact on wheat production. Plants are obligate aerobic organisms and they need to absorb oxygen from their surroundings to complete their life cycle, growth, reproduction and competition. With 215 million hectares, wheat has the largest area of cultivated plants in the world, waterlogging stress usually damages winter wheat during Seedling establishment and tillering. a field experiment in the form of a split plot design, based on randomized complete blocks in 3 replications accomplished. Stress was applied at the three-leaf stage and at three levels, control (no waterlogging stress), mild stress (48 hours) and severe stress (120 hours) as the main factors. Cultivars and genotypes were also included as secondary factors. Plants were cultivated in pots and grown in open space (outdoor), the water level was maintained about five centimeters above the soil level during the stress period. Results and DiscussionMild and severe waterlogging stress resulted in a decrease of 14.06 and 38.37 percent of shoot dry matter, respectively, which was significant in all cultivars and genotypes. Different cultivars and genotypes showed different responses to waterlogging stress. to better understand the reasons for these differences, among 11 cultivars and 10 genotypes, Mehrgan and Sarang cultivars and ms 93-16 and ms 93-6 genotypes were selected to study some physiological traits. Waterlogging stress led to a decrease photosynthetic pigments, an increase in the malondialdehydeconcentration and an increase in the antioxidant enzymes activity, and in these cases, the differences between these cultivars and genotypes were obvious. There were also differences between severe stress and mild stress, for example, in all studied cultivars, only severe stress could lead to a significant increase in peroxidase enzyme activity, but in the case of catalase, mild stress tolerant cultivars also led to a significant increase. ConclusionIn general, cultivars that had a higher level of antioxidant enzyme activity when faced with waterlogging stress were able to pass through the stress period with less damage to the cell membrane structure (according to the concentration of malondialdehyde) and photosynthetic pigments and showed a lower percentage reduction in dry matter. Mehrgan cultivar had the highest dry matter yield compared to other cultivars and genotypes in all three stress levels, despite the severe decrease in shoot system dry matter yield.

Effect of Biological and Chemical Fertilizers and Salicylic Acid Spraying on Yield and Some Physiological Traits of Wheat Under Drought Stress

Fatemeh Chaharlang Badil; khosrow Azizi; Hamid Reza Eisvand; Ali heidar Nasrollahi; Ahmad Ismaili

Volume 45, Issue 3 , December 2022, , Pages 397-408

https://doi.org/10.22055/ppd.2022.40567.2026

Abstract

IntroductionWheat (Triticum aestivum L.) is one of the most important and strategic crops worldwide. Drought stress is one of the important and abiotic stresses that reduces the growth ... Read More IntroductionWheat (Triticum aestivum L.) is one of the most important and strategic crops worldwide. Drought stress is one of the important and abiotic stresses that reduces the growth and yield of plants.in arid and semi-arid regions. Today, the economic damage and destructive effects of the environment due to the excessive use of chemical fertilizers in agriculture are known worldwide, and it is obvious that a suitable alternative must be found for these fertilizers. Currently, biofertilizers are used as an alternative to chemical fertilizers based on the principles of sustainable agriculture and stress tolerance of plants. Salicylic acid is a natural phenolic compound that is involved in reducing the harmful effects of abiotic environmental stresses and regulating vital physiological processes in plants. Materials and MethodsThis study was carried out to assess the effect of fertilizer nutrition and foliar application of salicylic acid on wheat tolerance of Chamran 2 cultivar to drought stress experiment as a factorial split plot in the form of a randomized complete block design in the 2019-2020 crop year in the research farm of the Faculty of Agriculture of Lorestan University in three replications was performed The experimental site was located at a latitude 33°26′ N, a longitude 45°17′ E, and an altitude of 1210 m with a semi-arid climate, mean annual precipitation of 462.8 mm, mean annual evaporation of 1842.52 mm, and mean annual temperature of 16.6 °C .Main plot includes irrigation levels in two levels of without stress (100% of field capacity) and drought stress (50% of field capacity) and sub-plots including fertilizer in five levels including no use of biofertilizer + 50% chemical fertilizer, application of 100% chemical fertilizer, seed inoculation of nitroxin biofertilizer + 50% chemical fertilizer, seed inoculation of mycorrhizal + 50% chemical fertilizer, (simultaneous use of biofertilizers (nitroxin + mycorrhiza) + 50% chemical fertilizer, and foliar application treatment at two levels (foliar application with water and foliar application with a concentration of 1 mM salicylic acid). Results and DiscussionThe results showed that drought stress increased grain protein and malondialdehyde and decreased other evaluated traits. Fertilizer nutrition and salicylic acid foliar application treatment alone increased the evaluated traits and decreased malondialdehyde content compared to the no use of biofertilizer + 50% chemical fertilizer and no foliar application of salicylic acid. Combined treatment of fertilizer (nitroxin + mycorrhiza) + 50% chemical fertilizer and salicylic acid could reduce the effect of drought stress on the traits evaluated in this study on wheat of Chamran 2 cultivar. As a result can this treatment suggested to the farmer in order to reduce the consumption of chemical fertilizers in the cultivation of this wheat variety under drought stress conditions. ConclusionIn general, it can be concluded that the application of biofertilizers (nitroxin + mycorrhiza) + 50% chemical fertilizer and salicylic acid foliar application can be used as an effective and alternative fertilizer to reduce the use of chemical fertilizers and water shortage in Khorramabad for sustainable agriculture. As a result, this treatment can be recommended to the farmer to reduce the use of chemical fertilizers in the cultivation of this wheat cultivar under drought stress conditions.

Comparison of Physiological Indices in Response to Cold Stress in Wild Tomato (Solanum habrochaites) for Screening of Cold Tolerant Lines

Zahra Nemati; seyed hosein nemati; Amin Mirshamsi Kakhki; Jafar Nabati

Volume 44, Issue 3 , December 2021, , Pages 395-406

https://doi.org/10.22055/ppd.2020.31274.1833

Abstract

AbstractIntroductionMany plants grown at low temperature exhibit different adaptation strategies involving physiological, ecological and morphological processes. Solanum lycopersicum ... Read More AbstractIntroductionMany plants grown at low temperature exhibit different adaptation strategies involving physiological, ecological and morphological processes. Solanum lycopersicum L. is affected by cold stress at all stages of growth; however, wild tomato species such as S. habrochaites rapidly improve after exposure to the cold stress through various changes in physiological processes. In this study, the short-term stress method was used for rapid screening and cold tolerance selection of tomato lines instead of long-term stress at 4 °C. Materials and MethodsThis study was carried out as a factorial experiment based on completely randomized design with three replications at Ferdowsi University of Mashhad Iran in 2018. Experimental factors included two levels of cold stress (0 and 2 °C) and three tomato lines (LA1777, LA3969 and LA4024). In the study, electrolyte leakage, MDA, anthocyanin, phenol, 1,1-diphenyl-2-picrylhydrazyl DPPH, total soluble carbohydrate content, the maximum efficiency of photosystem II and the activity of the three antioxidant enzymes CAT, POD, and APX were measured. Results and DiscussionAccording to the results of this experiment, LA1777 line showed the lowest leaf wilting after exposure to low temperatures. There was a significant difference between these lines in terms of electrolyte leakage. Under cold stress conditions, lowest electrolyte leakage and malondialdehyde (MDA) were obtained from wild line LA1777 that compared to sensitive line LA4024. LA1777 and electrolyte leakage at LA4024 at 0 and 2˚C were 14 and 11% higher than LA1777, respectively. Inhibition of DPPH radical activity of tolerant line LA1777 were higher than sensitive line LA4024 at 0 and 2 °C (43 and 45% respectively). Anthocyanin content of LA1777 and LA3969 lines were 49% higher than LA4024 at 0 °C. Under cold stress, the activity of the three antioxidant enzymes CAT, POD, and APX significantly increased in wild line LA1777, indicating better hydrogen peroxide scavenging system. After cold stress, LA1777 and LA3969 had the highest maximum efficiency of photosystem II, respectively, in light-adapted conditions (F'v / F'm), which may be due to higher cell membrane stability. The results showed that instead of long-term cold stress at 4˚C, the short-term stress method can be used for rapid screening of tomato lines for cold tolerance selection. Assessment of physiological parameters such as chlorophyll fluorescence, electrolyte leakage, MDA level, DPPH radical scavenging, catalase, peroxidase and ascorbate peroxidase enzymes showed that these indices can be used to detect cold stress and to quantify the stress in tomato lines. ConclusionBased on our data, we conclude that this short-term stress method is a useful method for rapid assessment of tomato chilling tolerance. The physiological parameters used in this study showed adequate difference between tomato lines.

The Comparison of Some Effective Physiological Characters to Improve Cold Tolerance of Seeded and Seedless Grapevine Cultivars During the Dormancy and Growing Seasons

Mousa Rasouli; Parvaneh Rustaei; Arash Babaei

Volume 41, Issue 4 , March 2019, , Pages 125-139

https://doi.org/10.22055/ppd.2018.21713.1466

Abstract

Abstract Background and Objectives The best way to increase the tolerance of plants against stresses is to know the specific characteristics of the cultivars, which is needed ... Read More Abstract Background and Objectives The best way to increase the tolerance of plants against stresses is to know the specific characteristics of the cultivars, which is needed both for planting in different climatic conditions and for breeding programs of these cultivars. For this reason, the exact study of the physiological traits of native cultivars of each region in the various stages of growing of grape is necessary. Materials and Methods Sampling was done in five times. During the dormancy season (Winter 2013) three times samplings of stems with buds were done and biochemical parameters of proline, electrolyte leakage and soluble sugar were measured. The aim of this study was to investigate some of the effective parameters of cold tolerance in five cultivars of grapevine including “Bidaneh Sefid”, “Bidaneh Ghermez”, “Lal”, “Sahebi” and “Fakhri” in a randomized complete block design (RBCD) with three replications. In the growing season (spring 2013) two times samplings were collected from the leaves of cultivars and biochemical parameters such as proline, malondialdehyde (MDA), soluble sugars, photosynthesis pigments, total protein, activity of antioxidant peroxidase, polyphenol oxidase and ascorbate peroxidase enzymes were measured. Results Result showed that there were significant differences between the cultivars in both winter and spring and in all considered characteristics. The winter cultivar of 'Fakhri' with minimal electrolyte leakage in all three times of sampling (33.12 µSiemens/gr Fw in January, 23.17 µSiemens/gr Fw in February and12.89 µSiemens/gr Fw in March) was more prepared to tolerate environmental stresses than other cultivars. In the spring, in both sampling times of spring season, cultivar of “Bidaneh Ghermez” with the least amount of malondialdehyde (3.7 µmol/gr FW in late April and 4 µmol/gr FW in early May) and the highest proline (10.27 µmol/gr FW late April and 7.39 µmol/gr FW in early May), and protein (respectively 0.26 mgr/gr FW late April and 0.23 mgr/gr FW in early May) were more prepared to tolerate environmental stresses than other cultivars that were examined in this study. The cultivar of “Lal” with the highest level of malondialdehyde (9.52 µmol/gr FW in late April and 4.52 µmol/gr FW in early May) showed lower relative stress endurance compared with other studied cultivars. In the winter, cultivar of 'Fakhri' had higher stress forbearance than other cultivars. Also, in the spring, cultivar of “Bidaneh Ghermez” had more and “Lal” had the lowest stress endurance than other cultivars that were examined in this study. Discussion Considering all the traits studied, it can be stated that in winter tolerance of seeded cultivars is more suitable than seedless cultivars. Seedless cultivars are more sensitive to temperature variations and generate severe reactions.

The effect of 5-aminolevolinic acid on some physiological responses of coriander (Coriandrumsativum L.) under drought stress

F. Ghanbari; M. Sayyari; M. Saidi; A.A. Amirinejad

Volume 37, Issue 1 , June 2014, , Pages 93-105

Abstract

In this study, the effect of 5-Aminolevolinic acid (ALA), a key precursor in the biosynthesis of porphyrin and the new plant growth regulator, was studied on some physiological responses ... Read More

Effects of UV-Radiation on Some Physiological Parameters in Capsicum longum L.

S H; Z K; J KH

Volume 34, Issue 2 , March 2012, , Pages 27-40

Abstract

The objective of this work was to evaluate the effects on some physiological parameters of pepper plants (Capsicum longum L.) exposed to UV radiation in the greenhouse. Pepper plants ... Read More

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Keywords = Malondialdehyde

Phytochemical changes of Denak (Oliveria decumbens) in response to salinity stress and application of salicylic acid hormone

Abstract

Effect of waterlogging stress at the three-leaf stage on the growth and some physiological characteristics of bread wheat (Triticum aestivum L.)

Abstract

Effect of Biological and Chemical Fertilizers and Salicylic Acid Spraying on Yield and Some Physiological Traits of Wheat Under Drought Stress

Abstract

Comparison of Physiological Indices in Response to Cold Stress in Wild Tomato (Solanum habrochaites) for Screening of Cold Tolerant Lines

Abstract

The Comparison of Some Effective Physiological Characters to Improve Cold Tolerance of Seeded and Seedless Grapevine Cultivars During the Dormancy and Growing Seasons

Abstract

The effect of 5-aminolevolinic acid on some physiological responses of coriander (Coriandrumsativum L.) under drought stress

Abstract

Effects of UV-Radiation on Some Physiological Parameters in Capsicum longum L.

Abstract