Plant Productions

The Impact of Pseudomonas putida and Chemical Phosphorus Fertilizer on Some Vegetative, Reproductive and Biochemical Characteristics of Two Species of Basil (O. sanctum L and O. basilicum var. thyrsiflora)

Naeimeh Enayatizamir; Mohammad Mahmoodi Sourestani; Mojtaba Momeni Monfared

Volume 44, Issue 2 , August 2021, , Pages 235-246

https://doi.org/10.22055/ppd.2020.31636.1844

Abstract

AbstractIntroductionOcimum basilicum L. (O. basilicum) or sweet basil belongs to the family Lamiaceae which has been used as a medicinal plant and its essential oils are used to flavor ... Read More AbstractIntroductionOcimum basilicum L. (O. basilicum) or sweet basil belongs to the family Lamiaceae which has been used as a medicinal plant and its essential oils are used to flavor foods, in dental and oral products and in fragrances. One of the methods to increase yield and availability of minerals in the soil, with the decreased use of inorganic fertilizers, is to apply biological fertilization, which also affects the essential oil of aromatic plants. Hence, this study was carried out to describe detailed growth and chemical investigation of basil essential oil affected by chemical and biological fertilization. Materials and MethodsThis study was carried out as factorial based on completely randomized design with three replications. The first factor involved plant (O. basilicum var. Thyrsiflora and O. sanctum.L ) and the second factor was fertilizer types (0%, 50% and 100% of the plant phosphorus requirement, only bacteria, bacteria with 50% of plant phosphorus requirement, bacteria with 100% of plant phosphorus requirement). The pots filled with five kilograms of soil. Phosphorus fertilizer was added to the pots from the triple super phosphate source based on the experimental treatments. Overnight culture of Pseudomonas putida in nutrient broth was prepared and applied under each seed. At full bloom stage, the leaves and inflorescences of each plant were completely separated and their number counted. Extraction of leaf samples was performed using 70% methanol solvent to measure phenolic compounds and the flavon and flavonol content of the extract was read by measuring the absorbance at 425 nm. The amount of phosphorus content of plant leave was also determined. Results and DiscussionShoot dry weight, number and length of inflorescence were significantly (p<0.01) affected by the interaction effect of fertilizers and plant species. The highest shoot dry weight, number and length of inflorescence were recoded with only bacteria without the use of phosphate fertilizer treatment and followed with samples treated with bacterial + 50% chemical fertilizer. The maximum phosphorus concentration in both basilicum and sanctum leaves (4.92 and 4.82 mg g-1 dry weight, respectively) and phenols, flavonoids and flavonoid compounds were obtained in treatments containing only bacteria without using phosphate fertilizer and followed with samples treated with bacterial + 50% chemical fertilizer, as well. The highest percentage inhibition of free radicals was recorded in basilicum and sanctum (67 and 75 percent respectively), in the presence of the bacteria without using phosphorus fertilizer. Applied bacterium in this research improved the quantity and quality of basil, thus to recommend it as a biological fertilizer considering its potential needs to be examined at farm conditions. ConclusionAccording to the results, using biofertilizer in this research reduced the demand for phosphorous fertilizer and improved the quantity and quality of basil; so as a result to recommend it as a biological fertilizer considering its potential needs to be examined at farm conditions.

Effect of postharvest treatment of gamma-amino butyric acid on some biochemical and antioxidant properties of sweet cherry cv. Tak Daneyeh Mashhad

H. Hassanpour; A. Bisti; S. Nojavan

Volume 41, Issue 2 , September 2018, , Pages 67-78

https://doi.org/10.22055/ppd.2018.20300.1422

Abstract

Abstract Background and Objectives Sweet cherry fruit due to high water content and respiration rate is rotten in post-harvest period and hence has a short storage life. So, reducing ... Read More Abstract Background and Objectives Sweet cherry fruit due to high water content and respiration rate is rotten in post-harvest period and hence has a short storage life. So, reducing of respiration rate and delaying of senescence process are required for increasing of storage life. Gamma amino butyric acid (GABA) is a non-protein amino acid with low content in the cell in normal state. No study has taken into account the effect of GABA on the storage life of sweet cherry fruit and also, increasing of shelf life by natural compounds seems essential to maintain the quality. Therefore, this study was conducted with the aim of improving the post-harvest quality of sweet cherry fruit, increasing biochemical and antioxidant properties and improving fruit appearance using GABA with the purpose of replacing it with synthetic chemicals. Materials and Methods Fruits of sweet cherry cv. Tak Daneyeh Mashhad were harvested at commercial maturity from a commercial orchard in Urmia (Iran) and transported to laboratory immediately. GABA was used in three concentrations (5, 10 and 20 mM). Titration method was used for determination of Titratable acid (TA) and pH was determined by pH meter. TSS was determined by refractometer. Total antioxidant capacity was determined by ferric ions reducing antioxidant power assay (FRAP). Total phenolic content was determined by Folin ciocalteu method and total flavonoid content was determined by the aluminum chloride colorimetric method. Results The results showed that TA content remains constant with treatment of GABA during storage. GABA treatment leads to an increase in total antioxidant activity, total phenol, total flavonoid and activity of catalase and guaiacol peroxidase enzymes in the treated fruits compared to control fruit after 30 days of storage. The pH value in treated fruits compared to untreated fruits was decreased during storage. Also, GABA at 10 mM level compared with other concentrations had the highest effect on total antioxidant capacity after 30 days of storage. Discussion The increasing of organic acids in treating fruits can be probably due to increasing of antioxidant capacity by GABA treatment, hence the damage to membrane was prevented and the organic acids were used less frequently. Treatment of fruits with GABA decreased the pH during storage, which may be due to its role in reducing of respiration rate in harvested fruits. The mechanism of the effect of GABA on increasing of total phenol and flavonoid content may be due to stimulating the production of Phenylalanine ammonia lyase (PAL) which can trigger phenylpropanoid pathway and hereby, syntheses of phenolic compounds such as flavonoids compounds were occurred. Catalase is one of the key enzymes to protect the cells and cause the conversion of hydrogen peroxide to water and oxygen. Therefore, probably the GABA treatment via the increasing the antioxidant system such as catalase leads to scavenge free radicals thereby protecting plants against oxidative stress.

Evaluation the genetic diversity of 10 Milk thistle (Silybum marianum L.) ecotypes using morphological, phenological and phytochemical traits

R. Hamid; M.R. Siahpoosh; R. Mamaghani; A. Siahpoosh

Volume 37, Issue 1 , June 2014, , Pages 37-47

Abstract

Understanding milk thistle genetic diversity is essential for breeding programs of this plant. In order to investigate genetic diversity of some Iranian ecotypes, an experiment was ... Read More

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Keywords = Flavonoid

The Impact of Pseudomonas putida and Chemical Phosphorus Fertilizer on Some Vegetative, Reproductive and Biochemical Characteristics of Two Species of Basil (O. sanctum L and O. basilicum var. thyrsiflora)

Abstract

Effect of postharvest treatment of gamma-amino butyric acid on some biochemical and antioxidant properties of sweet cherry cv. Tak Daneyeh Mashhad

Abstract

Evaluation the genetic diversity of 10 Milk thistle (Silybum marianum L.) ecotypes using morphological, phenological and phytochemical traits

Abstract