Plant Productions

Identification of Restoring Fertility and Maintainer Rice Varieties Using SSR Marker

GH. Kiani

Volume 40, Issue 1 , June 2017, , Pages 81-86

https://doi.org/10.22055/ppd.2016.12363

Abstract

Background and Objectives In classical plant breeding, restorers are identified by the cross test, of existing varieties with cytoplasmic male sterilty (CMS) lines and evaluating F1 ... Read More Background and Objectives In classical plant breeding, restorers are identified by the cross test, of existing varieties with cytoplasmic male sterilty (CMS) lines and evaluating F1 progenies in terms of fertility of pollen and spikelete. Lines showing more than 80 percent fertility of pollen and spikelete are considered as restorer lines. This study aimed at applying marker assisted selection (MAS) using linked SSR markers to restoring fertility (Rf) genes for identification of restorer and maintainer lines in hybrid seed production in rice. Materials and Methods In this study, 15 rice varieties wer planted at the research farm of Sari University of Agricultural Sciences and Natural Resources and marker assisted selection (MAS) was used for identification of two Rf genes located on chromosomes 1 and 10 of rice using SSR markers RM171, RM258 and RM3148. The PCR reaction was performed at 94 °C for 5 min; then for 35 cycles of 94 °C for 1 min; 55°C for 1 min; 72 °C for 2 min followed by 72 °C for 5 min. PCR products were resolved by electrophoresis in agarose gel stained with ethidium bromide and then photographed. Results Results of molecular analyses using microsatellite markers RM171, RM258 and RM3148 linked with Rf genes showed that rice varieties namely Hashemi and Deylamani had both Rf genes in their genomes and were considered as putative restorers and were suggested to be test crossed with male sterile lines to assess their F1 generations. Rice varieties namely Shiroudi, Tabesh, Fajr and Shafagh were identified as maintainers. Thus backcross breeding method is suitable for transferring sterile cytoplasm to them for development of new CMS lines. Discussions Identifying restorer lines by means of molecular marker technology is more cost effective and reliable than phenotypic assays especially in hybrid rice production. Restorer and maintainer lines identified in this study could be used for promotion of hybrid rice technology in the country.

The effect of chilling stress on anti-oxidative enzymes activity in two rice (Oryza sativa L.) cultivars

Z. Hasannejad; S.M. Seyyednejad; Abdol Ali Gilani; P. Hassibi

Volume 37, Issue 2 , September 2014, , Pages 27-37

Abstract

In order to investigate the chilling effect and consequently oxidative stress on anti-oxidant system of two rice cultivars a field experiment was conducted in Research Center of Agriculture ... Read More

Effect of water height on physiological and agronomical characteristics of rice cultivars in Ahvaz

A. Lotfi jala abadi; S.A. Siadat; G. Fathi; A. Ebdali; A. Gilani

Volume 36, Issue 3 , December 2013, , Pages 77-92

Abstract

In order to study the effect of plot water height on some agronomical and physiological characteristics of rice cultivars in Ahvaz, a field experiment was conducted experimental field ... Read More

Evaluation of Genes Effects and Providing Linkage Map in Iranian Rice Mapping Population of Gharib × Khazar Cross

H S; G M; A A

Volume 35, Issue 2 , September 2012, , Pages 29-52

Abstract

The genetic structure of agronomic traits has the most important role in determination of breeding methodology. Generation means analysis and QTL mapping refers to effective methods ... Read More

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Keywords = rice

Identification of Restoring Fertility and Maintainer Rice Varieties Using SSR Marker

Abstract

The effect of chilling stress on anti-oxidative enzymes activity in two rice (Oryza sativa L.) cultivars

Abstract

Effect of water height on physiological and agronomical characteristics of rice cultivars in Ahvaz

Abstract

Evaluation of Genes Effects and Providing Linkage Map in Iranian Rice Mapping Population of Gharib × Khazar Cross

Abstract