Plant Productions

The Dose Response and Mutation Induction by Gamma Ray in Vicia Faba Cv. Saraziri

Mohammad Reza Siahpoosh; Bahare Tahmasebi; Daryoosh Nabati Ahmadi; Masoud Rahimi

Volume 43, Issue 4 , December 2021, , Pages 517-526

https://doi.org/10.22055/ppd.2020.31265.1831

Abstract

ABSTRACT Introduction:Sufficient genetic variation is an essential source for having a successful breeding program. Mutation is known to be a high throughput technique to induce genetic ... Read More ABSTRACT Introduction:Sufficient genetic variation is an essential source for having a successful breeding program. Mutation is known to be a high throughput technique to induce genetic variation in plants. Irradiation is the most common method of inducing mutations in plants mutation breeding programs leading to the production of mutants with superior genotypes. Faba bean is a crop whose diversity needs to be improved for breeding programs in Iran. Unfortunately, few researches have been carried out on mutagenesis of faba bean. Materials and Methods:In the present study, the dose response and effects of gamma irradiation have been investigated by exposing the seeds of Vicia fabacv. Saraziri to doses of 25, 35, 45, 55, 100, and 120 Gray (Gy) in the Lab at the college of Agriculture, Shahid Chamran University of Ahvaz in 2013 to 2015. The implementation of the research was under the guidance and in cooperation with the Atomic Energy Organization of Iran. The experiments were assigned as a completed block design with four replications in both laboratory and the field. Results and Discussion:The results of the analysis of the variance indicated that there was no significant difference in germination trait of M0 generation in the seeds at various gamma irradiation doses whereas, germination rate, seedling length, and weight were significantly altered at varied doses (P≤0.05), and further responses of these traits decreased as the dosage increased. Moreover, increasing the irradiation doses caused a delay in flowering, pod setting, and pod ripening period in M2 generation. A linear regression between different characters and gamma doses was detected. Lethal dose, 50% (LD50) for fertility and seed setting, was detected as between 60-65 Gy based on reproductive traits. The principal component analysis revealed information that the first two components within the traits at the different doses were accounted for approximately 71% of the total variance. Using the biplot diagram of the first two components, 320 mutant plants appeared and the superior one within plants was identified as compared to control. Conclusion: This research is reported the optimum dose of gamma irradiation of 50 to 55 Gy to exert mutation induction in Vicia Faba based on the LD50 of vegetative and reproductive phases. The findings of the current work acquired several promising mutants that might be used as beneficial sources to develop new faba bean cultivars.

Investigating the Expression of Pinene Synthase and Linalool Genes in Response to Gibberellic Acid in Yarrow (Achillea millelium L.) Plant

Omid Poorraskari; Daryoosh Nabati Ahmadi; Khosro Mehdikhanlou; Leila Nejhadsadeghi

Volume 43, Issue 1 , June 2020, , Pages 13-24

https://doi.org/10.22055/ppd.2019.27042.1652

Abstract

Abstract Background and Objectives Currently, there is an immense attention towared medicinal plant and secondary motabiltes. Yarrow (Achillea millefolium L.) is a medicinal plant which ... Read More Abstract Background and Objectives Currently, there is an immense attention towared medicinal plant and secondary motabiltes. Yarrow (Achillea millefolium L.) is a medicinal plant which consists of two important compounds; including penene and linalool. Since these two compounds have the importance values, the researchers significantly focused on them. The rate of transcript expression of penene synthesis and Linalol synthesis induced at the end of 2-c-methyl-D-erythritol-4-phosphate (MEP) pathway that caused to produce Penene and Linalol. In order to evaluate the synthesis of Penene and Linalol, a research study was carried to exame the exogenous treatment (zero, 25 and 50 mg/l) of gibberellic acid (GA3) at different times (24, 48 and 74 hr.) on Yarrow at the greenhouse on 1395-1397. The results of R-PCR indicated that significant rate of the transcript expression was related to the Penene synthesis at 25 mg concentration of GA3 at the 48 hr after treatment. In the most samples as the rate of the Penene synthesis increased the rate of Linalol synthesis decreased. The evidences suggested that the exogenous treatment of GA3 induced a transduction signal in the plant. In which at the end, the plant molecular process respond to it and showed in overall that evaluation of GA3 implemented different encoding gene expression for theses compounds, and illustrated that when the Penene synthesis inceased in the treated plant the Linalol synthesis dcreased simontenously. Materials and Methods A greenhouse pot trial was conducted at the Experimental Research Station of College of Agriculture at Shahid Chamran University of Ahvaz. The plants used in this study were provided from the National Center for Genetic and Biological Resources of Iran, with access code P1000093. Two months after seed germination, the desired amounts of gibberellic acid were prepared with distilled water (25 mg/l and 50 mg/l), and then each potted plant was sprayed uniformly with 10cc of prepared solution. Sampling was carried out by collecting the leaves of treated plants at 24, 48 and 72 hours after the treatment. The phenol-chloroform method (Wang and Ghabrial, 2002) was conducted with some modifications to extract RNA of collected leaves at the two biological replications. Quantity and quality of extracted RNAs were measured using Nanodrop and electrophoresis of %1 agarose gel. Synthesis of cDNA was performed using the Takara cDNA synthetase kit according to its manufacturer's instructions. Partial sequences of pinene synthase and linalool synthase genes were obtained in an early experiment in our laboratory (data is not published). The coding sequence of the target genes in other plants within the same family aligned with our obtained sequences to identify conserved segments for each gene using CLUSTAL W program. The standard housekeeping actin for yarrow with accession number JX679606.1, which is available in the public GenBank database, used to normalize the expression of the genes of interest. The primers of all genes (pinene synthase, linalool synthase and actin) were designed from the conserved sequences by PrimerQuest Tool, and then verified with oligoanalyzer v.3.1. Relative expressions of target genes were determined using real-time PCR with SYBR green fluorescence detection. Relative differences in target gene expression were calculated using REST software. Results The acquired result of real-time PCR revealed that in pinene synthase gene, the highest amount of transcription occurred at 48 hours after the treatment with the concentration of 25 mg of gibberellic acid and, interestingly, the lowest expression of the transcript for linalool synthase gene observed at the same level of concentration and time. In most of the samples, with the increase in pinene synthase transcriptase, the amount of linalool synthase transcript decreased. Discussion Taking into account the fact that both pinene and linalool are synthesized from the common substrate geranyl diphosphate. Therefore, an increase in the expression one leads to reduction in the transcript expression of the other one in the same pathway. Our results also confirmed that the transcription level of pinene synthase gene has been increasingly altered in response to the applied concentrations of exogenous gibberellic acid, resulting in a reduction in the expression of linalool synthase gene. Evidence suggests that the application of gibberellic acid triggers a cascade of molecular events which ultimately constitutes the plant response to the elicitor.

Path Analysis to Study Morph-Physiological Traits, Yield and Traits Related to Yield of Lentil Genotypes under Rain fed Condition

Fatemeh Zahedi; D N; Mohtasham Mohammadi; Rahmat allah karimizadeh

Volume 39, Issue 2 , September 2016, , Pages 71-80

https://doi.org/10.22055/ppd.2016.12056

Abstract

Background and Objectives Statistical parameters can be used in plant research to elucidate the differences in growth behavior among traits in a plant population. Materials and Methods ... Read More

Comparison of Different Genomic RNA Extraction Methods From the Medicinal Plant Yarrow (Achillea millefolium L.)

Maryam Javdan Asl; Hamid Rajabi Memari; Daryoosh Nabati Ahmadi; Afrasiyab Rahnama

Volume 39, Issue 2 , September 2016, , Pages 105-114

https://doi.org/10.22055/ppd.2016.12050

Abstract

Background and Objectives RNA extraction is considered a crucial step in the molecular studies and the results of subsequent work such as, PCR, Real-TIME PCR and RACE PCR depend on ... Read More

Determination of plant diversity using morphological characters and path analysis in dill germplasms

Z. Fathalipoor; D. Nabati Ahmadi; H. Rajabi Memari; A. Siahpoosh; F. Sedighi Dehkordi

Volume 37, Issue 4 , March 2014, , Pages 57-67

Abstract

The most important composition character within a plant breeding program is to have type and amount of genetic diversity. In addition to using a visual approach and genetic tools to ... Read More

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Author = Daryoosh Nabati Ahmadi

The Dose Response and Mutation Induction by Gamma Ray in Vicia Faba Cv. Saraziri

Abstract

Investigating the Expression of Pinene Synthase and Linalool Genes in Response to Gibberellic Acid in Yarrow (Achillea millelium L.) Plant

Abstract

Path Analysis to Study Morph-Physiological Traits, Yield and Traits Related to Yield of Lentil Genotypes under Rain fed Condition

Abstract

Comparison of Different Genomic RNA Extraction Methods From the Medicinal Plant Yarrow (Achillea millefolium L.)

Abstract

Determination of plant diversity using morphological characters and path analysis in dill germplasms

Abstract