Plant Productions

Micropropagation of Polianthes tuberosa L. Through Direct Organogenesis

Mohammad Hosein Daneshvar; Mehri Havil; Amin Lotfi Jalal Abadi

Volume 45, Issue 2 , September 2022, , Pages 277-286

https://doi.org/10.22055/ppd.2022.36928.1973

Abstract

IntroductionPolianthes tuberosa L. is one of the most important ornamental bulbous plants in tropical and subtropical regions that is cultivating in some regions of Iran. Due to the ... Read More IntroductionPolianthes tuberosa L. is one of the most important ornamental bulbous plants in tropical and subtropical regions that is cultivating in some regions of Iran. Due to the transmission of fungal and bacterial diseases, propagation by bulbs is not affordable. Tissue culture is a good way to produce uniform and disease-free seedling. Therefore, in vitro cultivation of tuberose is a suitable solution to commercial reproduction. Materials and MethodsTo investigate the best method of micropropagation of Polianthes tuberosa, this study was conducted in three experiments (proliferation, root formation and adaptation). The explants used in this study were lateral buds. Ethyl alcohol (70%) and dilute laundry bleach (5.25% active chlorine), benomyl fungicide and dishwashing liquid were used to eliminate surface contamination of the explants. In the first experiment, the effect of different plant growth regulators and MS medium (MS, ½ MS, ¼ MS) on shoot formation of lateral bud was investigated. Lateral bud explants were cultured in MS medium with various plant growth regulators including KIN (0.25, 0.5, 0.1, 0.2 and 0.4 mg / L), BAP (0.25 (0.5, 0.1 and 2.0 mg / l) and then the length and number of shoots were evaluated. After forming shoots in the propagation medium, they were transferred to a new environment to form roots.The root formation experiment included tree levels of NAA concentrations, (0.5, 0.75 and 1 mg/L) in combination with 0.2 mg/L IBA and control (without growth regulator). Adaptation experiment with 5 Substrate of bed including Peat Moss, Cocopeat, Sand, Peat Moss with Cocopeat (1: 1) and Sand with Cocopeat and Peat Moss (1: 1: 1) was conducted. Results and DiscussionThe results showed that there was a significant difference between various types of plant growth regulators (PGR) and MS culture media at different concentrations and no branch was produced without the use of PGR. In shoot prolifferation experiment, the maximum number of shoots (4.8) was obtained from lateral bud explants in MS medium with 0.25 mg / l BAP in combination with 0.25 mg / l KIN. The highest length of lateral shoot shoots (3.75 cm) was observed in MS medium with 0.25 mg / l KIN in combination with 0.25 mg / l BAP.The results of root formation experiment showed that the highest number of roots was observed in MS medium containing 0.5 mg / l NAA and 0.2 mg / l IBA and the lowest number of roots in control (without plant growth regulator). Treatment of 0.5 mg / l NAA with 0.2 mg / l IBA had the highest root length (1.22 cm). The lowest root length was also observed in the control (without plant growth regulator). In the adaptation test, no significant difference was observed between treatments in survival rate. ConclusionThe results of this study showed that the shoot organogenesis is controlled by the cytokinin with MS medium in different concentrations. It should be mentioned that cytokinin had an important role in the synthesis of RNA, stimulation of the production of protein and the activity of some enzymes. Also, the results showed that the use of cytokinin alone has a beneficial effect on shoot organogenesis.

In Vitro Regeneration of Ornamental Crocus (Crocus vernus L.) by Using Plant Growth Regulators

Sara Parvin; Mohammad Hosein Daneshvar; amin lotfi jalal- abadi

Volume 42, Issue 4 , December 2019, , Pages 429-440

https://doi.org/10.22055/ppd.2019.25898.1603

Abstract

Abstract Background and Objectives Crocus flower, which belongs to Iridaceae family, is one of the most important ornamental bulbous plants. The propagation of Crocus vernus ... Read More Abstract Background and Objectives Crocus flower, which belongs to Iridaceae family, is one of the most important ornamental bulbous plants. The propagation of Crocus vernus L. by corm is not commercially affordable due to transmission of fungal and bacterial diseases as well as the long dormancy period which takes 4 to 5 months. Thus, in vitro culture of Crocus vernus is a suitable solution for commercial reproduction. Plant tissue culture technique would be a better alternative for improving quality and production. Materials and Methods In order to micropropagate ornamental saffron, the effect of different culture media on direct organogenesis experiment was conducted in factorial arrangement in a completely randomized design with 3 replications in the tissue culture lab of the Department of Horticulture at Agricultural Sciences and Natural Resources University of Khuzestan from 2015 to 2016. The explants used in this experiment were latheral and terminal buds which were evaluated in 10 hormons treatments and control in the Murashige and Skoog medium (MS). To eliminate surface contamination, the explants were first immersed under water for 30 minutes after placing the corms. Next, they were placed in high temperature Benlate fungicide solution (55° C) for 30 minutes. Then, they were put in 70% ethanol (ethyl alcohol) for 30 seconds and 15% Sodium hypochlorite solution for 6 minutes. The explants were finally washed three times with sterile distilled water under the air flow bench. The effects of plant growth regulators including BAP (0.5, 1.0, 2.0 mg/l), TDZ (0.5, 0.75, 1.0 mg/l), Kin (0.5, 1.0, 2.0 mg/l) and 0.1mg/l IBA in direct regeneration were investigated. Results The results showed that the kind of plant growth regulators (PGR) and the explant type are very important in regeneration of Crocus vernus so that we can not secure any shoots without PGR. In direct organogenesis experiment, the maximum number of multiple shoots (15.84) was obtained from apical bud explant in MS medium supplemented with 5/0 mg/l TDZ along with 5/0 mg/l BAP, 5/0 mg/l KIN and 1/0 mg/l IBA, after 10 weeks, that had a significant difference with other treatments at 1% Duncan test. We observed 2.o mg/l BAP along with 1/0 mg/l IBA had the greatest effect on the number of corms. Discussion The results showed the shoot regeneration is controlled by the ratio of cytokinin with auxin. It should be mentioned that cytokinin plays a role in the synthesis of RNA, stimulation of the production of protein, and the activity of some enzymes. The results also indicated that the use of cytokinin along with auxin has a beneficial effect on shoot regeneration.

Utilization of Indirect Regeneration to Induce Micropropagation of Fritillaria imperialis L. on in vitro Culture

Mojtaba Rahimi; Mohammad Hosein Daneshvar

Volume 39, Issue 4 , March 2017, , Pages 31-42

https://doi.org/10.22055/ppd.2017.12066

Abstract

Background and Objectives Iran is home of one of the most delightful and graceful flower of Fritillaria in the world. Its habitation is commonly expanded on the vast areas: growing ... Read More Background and Objectives Iran is home of one of the most delightful and graceful flower of Fritillaria in the world. Its habitation is commonly expanded on the vast areas: growing in beds, borders, backgrounds and rock gardens. Besides, it is also suitable for naturalizing and excellent for cut flowers. Fritillaria imperialis as a medicinal plant has pharmaceutical purpose in which possesses a health beneficial essence that used in production of mucus and cough medicine. In addition, extractions of plant tissues contain certain of properties which affect to reduce blood pressure and blood sugar. Unfortunately, the habitation of this ornamental plant is under threat and majority of its habitation already being to ruin due to human activities and urbanization. The propagation of Fritillaria through offsets (bulblets) is prolonging approach which takes approximately 3-4 years. Whereas, using tissue culture technique for flower production is required less time probably within a few months and economically has a huge advantage due to low cost and a numerous plant production in a short time. Materials and methods This research was carried out in four experiments: callus formation, bulblet production, root formation and acclimatization. In the present study, a factorial experiment was assigned as completed randomized design (CRD) with three replications. In the first experiment, leaf primordia and scale explants were cultured on MS medium supplemented with different concentrations of NAA and IBA in order to produce callus formation. In the second experiment, the calli were cultured on organogenesis medium which were contained MS medium supplement, with kin. and TDZ, after 6 and 12 weeks. In the third experiment, the bulblets were transferred into rooting media, which were contained MS medium supplemented with different concentrations of NAA (0, 0.5, 1.0 and 2.0 mg/l). In the fourth experiment, after washing the bulblet whit warm distilled water (40ᵒC) that contain 2.0 mg/l benlate fungicide, they then were planted into the pots containing sterilized perlite, cocopeat and perlite along with cocopeat. Results In the first experiment, the results demonstrated that the highest weight of callus (3.29g) was sustained in scale explants on basal MS medium which was supplemented with 0.5mg/l NAA along with 0.5mg/l IBA. In second experiment, the result indicated that the numerous of bulblets were developed on MS medium supplemented with 0.5mg/l TDZ along with 0.5mg/l kin. Which of these, the callus scale were produced on MS medium supplemented with 0.5mg/l NAA. In the third experiment, in order to retain rooting the bulblets, they were cultured on MS medium supplemented with different concentration of NAA. The result elucidated that the large quantity of roots (7.7) were obtained from leaf primordia on MS medium supplemented with 1.0mg/l NAA. In the fourth experiment, rooted bulblets were washed with warm distilled water for 5-10min, and then they were transferred into pots with perlite, cocopeat and perlite + cocopeat. Discussion The results revealed information that the rooted bulblets from scale growing on cocopeat substrate had retained the most diameter (21.64mm) as compared to other treatments. The finding results under this investigation strongly suggested that using micropropagation it is definitely a possible trend to abolish the barriers which caused to deteriorate of Fritillaria imperiallis.

Effects of Calcium Nitrate on Nitrate Reductase Activity, Amino Acids, Nitrate and Ion Accumulation of Pistacia Vera L., Badami Zarand, Under Sodium Chloride Stress

S. Nasseri Khollari; M. Heydari; S. Jafari; M.H. Daneshvar

Volume 38, Issue 4 , March 2016, , Pages 35-48

https://doi.org/10.22055/ppd.2016.11833

Abstract

Nitrogen is considered as an essential nutrient for plants and could be taken up in large amounts by plants. Nitrogen fertilizers are known to have beneficial effects when added to ... Read More

Effect of growth regulators and antioxidants in the medium on in vitro callus induction in European yew (Taxus baccata L.)

A. Abbasi kajani; K. Alami Saeid; M.H. Daneshvar; M.R. Mofid

Volume 37, Issue 4 , March 2014, , Pages 1-7

Abstract

Yew plant is major source of Taxol, highly effective anti cancer medicine, and cell culture is one way to increase its production. A factorial experiment using B5 as base medium was ... Read More

The effects of different levels of sodium chloride and mycorrhizal colonization on growth, P, K and Na uptake by saffron (Crocus sativus L.)

H. Nadian; M. Heidari; M.H. Gharineh; M.H. Daneshvar

Volume 36, Issue 2 , September 2013, , Pages 49-59

Abstract

The effect of different levels of salinity arising from sodium chloride on plant growth parameters, phosphorus, potassium and sodium uptake by saffron plant with and without mycorrhiza ... Read More

Effect of row planting design and planting distance on flowering and quality of gladiolus cut flower (Gladiolus hybrida cv. Oscar (

M.H. Daneshvar; M. Heidari

Volume 36, Issue 1 , June 2013, , Pages 129-140

Abstract

The increasing of planting density of bulbous flowers is one of the most important step for more production of ornamental plants. One of the possible methods for increasing density, ... Read More The increasing of planting density of bulbous flowers is one of the most important step for more production of ornamental plants. One of the possible methods for increasing density, is cultivation of bulb plants in multiple rows pattern. In this research, the effect of one, two, and three row planting design bed were investigated on flowering and quality of Gladiolus (Gladiolus hybrida cv.Oscar) cut flower in climatic condition of Mollasani in Khuzestan province, located in south west of Iran on 1388 through 1389.. The treatments were the cultivation of corms in on row of 30 × 20 cm (control) and two and three-bed row (20 cm on row, 30 cm between main row and/or pattern with distance of 15, 20 and 25 cm between subordinate row). The results showed that cultivation of corm in three-row bed pattern with distance of 25 cm between subordinate row and/or cultivation of two-bed and three-bed row in distance between subordinate rows of 15 and 20 cm causes significant decreases on weight of cut flower, stem diameter and height of spike. Height of cut flower was not significantly different only in treatment of two-bed row with distance of 25 cm between subordinate rows when compared to control. The number of cormels were significantly decreased In treatment of three-bed cultivation pattern with distance of 25 cm between subordinate rows, as compared to control. The number of floret, only, in cultivation pattern of two-bed and three-bed row with distance of 15 cm between rows had significantly decreased when compared to control treatment. These treatments had no significant effect on diameter of cut flower, height of spike, diameter, width and weight of corms. The results indicated that the cultivation of gladiolus in pattern of multiple rows is possible but the decrease of distance between rows, reduce the quality of cut flower. It strongly suggested that more researches require to accomplish on various distances on subordinate rows.

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Author = Mohammad Hosein Daneshvar

Micropropagation of Polianthes tuberosa L. Through Direct Organogenesis

Abstract

In Vitro Regeneration of Ornamental Crocus (Crocus vernus L.) by Using Plant Growth Regulators

Abstract

Utilization of Indirect Regeneration to Induce Micropropagation of Fritillaria imperialis L. on in vitro Culture

Abstract

Effects of Calcium Nitrate on Nitrate Reductase Activity, Amino Acids, Nitrate and Ion Accumulation of Pistacia Vera L., Badami Zarand, Under Sodium Chloride Stress

Abstract

Effect of growth regulators and antioxidants in the medium on in vitro callus induction in European yew (Taxus baccata L.)

Abstract

The effects of different levels of sodium chloride and mycorrhizal colonization on growth, P, K and Na uptake by saffron (Crocus sativus L.)

Abstract

Effect of row planting design and planting distance on flowering and quality of gladiolus cut flower (Gladiolus hybrida cv. Oscar (

Abstract