ریزازدیادی پایه رویشی پنتا (Prunus domestica) در دو محیط ‌کشتMS و B5

نوع مقاله: علمی - پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد بیوتکنولوژی کشاورزی، دانشکده کشاورزی و منابع طبیعی، دانشگاه گنبدکاووس‌، گنبد کاووس، ایران

2 استادیار، گروه تولیدات گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه گنبدکاووس، گنبد کاووس، ایران

3 گروه تولیدات گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه گنبد کاوس، گنبد کاوس، ایران

چکیده

چکیده
پژوهشی به‌ منظور بررسی ریزازدیادی پایه پنتا (Prunus domestica)در دو محیط ‌کشتMS  و B5 انجام گرفت. این آزمایش به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با 5 تکرار انجام شد. در این پژوهش غلظت‌های مختلف  هورمون ایندول بوتیریک اسید (صفر، 5/0، 9/0 و 5/1 میلی‌گرم در لیتر) برای ریشه زایی و نیز غلظت‌های مختلف  6-بنزیل آمینو پورین (0، 4/0، 8/0 و 2/1 میلی‌گرم در لیتر)برای شاخه زایی در دو محیط کشت مورد بررسی قرار گرفتند. صفات مورد مطالعه شامل تعداد برگ، طول ساقه، تعداد شاخه، تعداد ریشه و طول ریشه بود. نتایج تجزیه واریانس اختلاف معنی‌داری بین محیط‌های کشت، سطوح هورمون و اثر متقابل آن‌ها برای صفات مورد مطالعه نشان داد. بر این اساس بیش‌ترین طول ساقه (39/6 سانتی‌متر) و تعداد شاخه (65/13 عدد) به ترتیب در محیط ‌کشت  MSدارای 8/0 و 4/0 میلی‌گرم در لیتر BAP حاصل شد. در این آزمایش بالاترین میانگین تعداد ریشه و طول ریشه به ترتیب 41/8 عدد و 75/14 سانتی‌متر در محیطB5  و  MSبا استفاده از غلظت 9/0 و 5/1 میلی‌گرم در لیترهورمون IBA به دست آمد. نتایج نشان داد گیاهچه‌های رشد یافته در محیط ‌کشت MS دارای رشد بهتری نسبت به گیاهچه‌های رشد یافته در محیط ‌کشت B5 بودند که می‌تواند به دلیل وجود مقادیر بالای نیتروژن در محیط کشت MS باشد.از این پژوهش می‌توان نتیجه گرفت که برای شاخه‌زایی پایه پنتا، محیط کشت MS  با هورمون 4/0 میلی‌گرم در لیتر BAP و برای ریشه‌زایی محیط کشت  B5 با 9/0 میلی‌گرم در لیتر هورمون IBA مناسب می‌باشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Micro Propagation of Penta Rootstock (Prunus domestica) in the Two Culture Media (MS and B5)

نویسندگان [English]

  • Ziba Balapoor 1
  • Hossein Hosseini Moghaddam 2
  • Mehdi zarei 2
  • mehdi Mollashahi 3
1 1- M.Sc. Student of Biotechnology, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran
2 Assistant Professor, Department of Plant Production, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran
3 Dept. of plant production, Faculty of Agriculture and Natural resources, Gonbad kavous university, Gonbad Kavous, Iran
چکیده [English]

Abstract
 
Background and Objectives
Culture medium and growth regulators type can influence the growth traits and for determining the most effective medium and hormone compositions an extensive studies are needed; otherwise the culture medium may have a negative effect on the growth traits. In this research the effects of two culture media (B5 and MS) supplemented with two growth regulators (IBA and BAP) with different concentrations (0.5, 0.9, and 1.5 for IBA and 0.4, 0.8, and 1.2 mg/l for BAP) alone or in combination was evaluated. Studied traits were number of leaf per shoot, shoot length, number of regenerated shoots based on completely random plan with 5 replications.
 
Materials and Methods
Explants provided in the beginning of spring from mother plants growing in open environment. Shoot tips and axillary buds (0.3-0.4 cm long) used as the explants. After washing with tap water, explants were immersed in ethylic alcohol for few seconds (3-4 seconds) then immersed in mercuric chloride (0.005 W/V) for 5 minutes which supplemented with few drops of tween 20 and finally washed three times with sterile distilled water. Disinfected explants cultured on medium under air laminar flow bench. Growth chamber was adjusted to 16 hours light and 25 to 27ᵒC.
 
Results
The results of variance analysis demonstrated significant differences between media, hormone level and interaction between hormone concentrations for traits studied. The results showed the longest shoot (6.39 cm) and the highest shoot number (13.65) obtained in MS medium supplemented with 0.8 and 0.4 mg/l BAP respectively. In this experiment the highest root number (8.41) and root length (14.75 cm) was observed in B5 and MS medium containing 0.9 and 1.5 mg/l, IBA.
 
Discussion
Overall the most shoot proliferation of this rootstock occurred in MS medium. New shoots in MS medium showed normal and better growth comparing to shoots obtained in B5 medium, that can be one of the results of higher amount of nitrogen (ammonium nitrite) in MS medium. In B5 medium with all hormone treatments weak growth observed for this plant. In fact this medium was made for herbaceous plants and this is one of the main reasons why penta rootstock did not showed good growth symptoms on this medium.

کلیدواژه‌ها [English]

  • Adaptation
  • BAP
  • IBA
  • Micro propagation
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