بررسی اثر غلظت‌های مختلف کلرید کادمیوم بر تنش اکسیداتیو گیاه بادرنجبویه تحت شرایط درون شیشه‌ای

نوع مقاله: علمی - پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد تولیدات گیاهی، دانشکده کشاورزی، دانشگاه ملایر، ملایر، ایران

2 دانشیار، گروه زیست‌شناسی، دانشکده علوم، دانشگاه شهرکرد، شهرکرد، ایران

3 استادیار، گروه زراعت، دانشکده کشاورزی، دانشگاه ملایر، ملایر، ایران

چکیده

چکیده
بادرنجبویه(Melissa officinalis L.)گیاه دارویی متعلق به خانواده نعناعیان (Lamiaceae) است. فلزات سنگین یکی از عوامل اصلی تنش غیر‌زیستی برای موجودات زنده می‌باشند.کادمیوم یکی از فلزات سنگین غیرضروری است که با تحریک سنتز رادیکال‌های آزاد اکسیژن در گیاه منجر به ایجاد تنش اکسیداتیو می‌گردد. این پژوهش در سال‌های 1396-1395 در دانشگاه شهرکرد انجام شد. در این مطالعه اثر غلظت‌های مختلف کلرید کادمیوم(صفر، 10، 20 و 40 میکرومولار)بر شاخص‌های بیوشیمیایی گیاه بادرنجبویهموردبررسی قرار گرفت. به این منظور از ساقه‌های گیاه بادرنجبویه که در شرایط استریل بر روی محیط کشت موراشیگ و اسکوگ (1/2 MS) تکثیر یافته بود استفاده شد. ساقه گیاهچه‌های دوماههدر محیط کشت مایع (1/2 MS) حاوی غلظت‌های مختلف کلرید کادمیوم کشت گردید.پس ازگذشت یک هفته، نمونه‌برداری از آن‌ها جهت انجام آزمایش‌ها صورت گرفت. بر اساس نتایج به‌دست‌ آمده در این مطالعه وزن ترساقه‌های کشت‌شده در غلظت‌های 10 و 20 میکرومولار کلرید کادمیوم نسبت به شاهد افزایش پیدا کرد. این فلز سنگین به‌طور معنی‌داری سبب کاهش مقدار کلروفیل a، b وکارتنوئیدها شد در حالیکه میزان کلروفیل کل  تنها در غلظت 40 میکرومولار نسبت به شاهد کاهش پیدا کرد.همچنین کادمیوم به‌طور معنی‌داری سبب افزایش میزان پراکسید هیدروژن H2O2 در تمامی غلظت‌های تیمار شد. در بین آنزیم‌های آنتی اکسیدان مورد بررسی، کلرید کادمیوم موجب افزایش فعالیت آنزیم‌های کاتالاز و سوپر‌اکسیددیسموتاز شد. میزان مالون دی‌آلدئید در تمامی غلظت‌های تیمار افزایش پیدا کرد اما در غلظت 40 میکرومولار این افزایش نسبت به شاهد معنی‌دار نبود. کلریدکادمیوم موجب کاهش جذب مولیبدن و آهن شد. از طرفی باعث افزایش جذب منگنز و روی گردید. بنابراین می‌توان نتیجه‌گیری کرد که احتمالاً کلرید کادمیوم در ساقه‌های بادرنجبویه باعث تجمع ترکیباتی مثل پراکسید هیدروژن شد که به‌عنوان یک مولکول پیام‌رسان باعث القای دفاع آنتی‌اکسیدانی گردیده است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Effect of Different Concentrations of Cadmium Chloride on Oxidative Stress in Shoot Cultures of Lemon Balm

نویسندگان [English]

  • Sayyedeh Razieh Nourbakhsh Rezaei 1
  • Leila Shabani 2
  • Majid Rostami 3
  • Mohammad Abdoli 3
1 M.Sc. Graduate of Plant Production, Faculty of Agriculture, Malayer University, Malayer, Iran
2 Associate Professor, Department of Biology, Faculty of Science, Shahrekord University, Shahrekord, Iran
3 Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Malayer University, Malayer, Iran
چکیده [English]

Abstract
 
Background and Objectives
Melissa officinalis is a medicinal plant belonging to the Lamiaceae family. The essential oil of this plant is used in many fields. Although more than 100 types of chemical substances have been identified in this plant, the most important compositions of its essential oil include citral, citronella, geraniol and linalool. Heavy metals are one of the main causes of non-biotic stress for living organisms due to increased use in the field of industrial and agricultural development and its high accumulation and toxicity. Cadmium is an unnecessary heavy metal, which, due to its high mobility and low concentration, easily enters the food chain from the soil. Cadmium induces oxidative stress by stimulating the synthesis of free oxygen radicals in the plant. In this study, the effect of cadmium chloride on oxidative stress induction in lemon balm was investigated. This experiment was carried out in 2016-2017 at Shahrekord University.
 
Materials and Methods
This research was conducted in a completely randomized design with three replications in vitro condition. We investigated the effects of different concentrations of cadmium chloride (0, 10, 20 and 40μm) on the biochemical parameters of the lemon balm. In this study, sterile stems of lemon balm propagated on the medium (1/2 MS) were used. After 60 days, seedlings were removed from solid MS medium and the roots were cut and cultured in liquid medium of 1/2 MS with different concentrations of cadmium. The cultivation was carried out in Erlenmeyer flask (250 cc) and was kept in an incubator shaker device. Sampling for the experiment was conducted one week after the growth of the stems in the medium. 
 
Results
Based on the results obtained in this study, fresh weight of shoots grown in 10 and 20μM of cadmium chloride increased compared to the control. This heavy metal significantly reduced the amount of chlorophyll a, b and carotenoids, while the total chlorophyll content decreased only at 40μm concentration relative to control. Cadmium significantly increased hydrogen peroxide levels in all treatments. Also, it increased the activity of catalase and superoxide dismutase enzymes. Malondialdehyde increased in all treatment concentrations, but this increase was not significant at 40μm of cadmium chloride. Chloride cadmium reduced the absorption of molybdenum and iron, yet increased the adsorption of manganese and zinc.
 
Discussion
The results of this study revealed that the cadmium chloride in lemon balm shoots may have the potential to accumulate compounds such as hydrogen peroxide which, as a messenger molecule, produces antioxidants and can help the plant tolerate stressed conditions. It seems that two concentrations of 10 and 20 μm of cadmium chloride have no toxic effects for the stems and the plant has managed to withstand this tension with defense mechanisms.

کلیدواژه‌ها [English]

  • Antioxidant enzymes
  • Cadmium
  • heavy metal
  • Melissa Officinalis
  • Tissue culture
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